Catalytic isomerization of glucose to fructose by immobilized glucose isomerase in an aqueous-ethanol medium was studied. Using Michaelis-Menten equation and Haldane relationship, the main kinetic parameters were determined: Kmf, Vmf, Kmr, Vmr. By comparing the aqueous-ethanol medium with an aqueous solution, the measured values of Kmr and Kmf were increased in the aqueous-ethanol solution, while the values for maximal velocities (Vmf and Vmr) were decreased in this medium. The results from these comparisons were discussed with reference to the rate constants in this isomerization reaction. The integrated form of the Michaelis-Menten equation was used with good approximation to predict the course of the isomerization reaction in the aqueous-ethanol solution.

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