Showing 3 results for Biocompatibility
M. Yousefpour,, A. Zareidoost , A. Amanzadeh,
Volume 30, Issue 1 (6-2011)
Abstract
The osseointegration of oral implants is related to the early interactions between osteoblastic cells and titanium surface. Chemical surface modification of titanium (Ti) implants is used to improve peri-implant bone growth, bone-to-implant contact, and adhesion strength. Thus, in this study, the surface topography, chemistry, and biocompatibility of polished titanium surface treated with mixed solution of three acids containing hydrochloric acid (HCl)- hydrofluoric acid (HF)- phosphoric acid (H3PO4) were studied under different concentration conditions. Moreover, Osteoblast cell (MG-63) was cultured on the and treated polished titanium surface. Also, in order to investigate titanium surface, SEM, AFM and EDS analyses were carried out. The results revealed that the surface of titanium treated with mixed solution containing the aforesaid acids had higher roughness, cell attachment, and proliferation than the controls
B. Karami, M. Imani, A. Seifalian, M.a.shokrgozar, S.bonakdar, A. Khavandi,
Volume 32, Issue 1 (6-2013)
Abstract
This study aims to characterize and evaluate the applicability of Polyhedral OligomericSilsesquioxane (POSS)/ Poly (carbonate-urea) Urethane (PCU) nanocomposite films as a temporary skin substitute by means of FTIR, MTT assay, cell proliferation assay and SEM studies. FTIR spectra showed all the characteristic peaks of POSS/PCU nanocomposite. The indirect cytotoxicity of membranes was investigated by MTT assay. In MTT test, L929 mouse fibroblasts were exposed to the extract of the films for 24 h. MTT results showed no sign of cell cytotoxicity for the extracts at the extraction times up to14 days. Menwhile, it was found that POSS nanocages have a stimulating effect on L929s. In cell proliferation assay, L929s were cultured on the films for 3, 7 and 14 days. The cells showed a high rate of proliferation in direct contact with the biomaterial after 7 and 14 days. Morphology and density of the cells on the nancomposite surface was investigated through SEM observations. SEM micrographs showed that the cells adhered well on the surface after 3 days of culture. Moreover, after 7 days, cell density increased so substantially that a cell layer was formed on the membranes.
E. Shirani, A. Razmjou,
Volume 36, Issue 4 (3-2018)
Abstract
The significance of producing superhydrophobic surfaces through modification of surface chemistry and structure is in preventing or delaying biofilm formation. This is done to improve biocompatibility and chemical and biological properties of the surface by creating micro-nano multilevel rough structure; and to decrease surface free energy by Fault Tolerant Control Strategy (FTCS) . Here, we produced a superhydrophobic surface through TiO2 coating and flurosilanization methods. Then, in order to evaluate the physicochemical properties of the modified surfaces, they were characterized by Scanning Electron Microscope (SEM), Fourier Transform Infrared Spectroscopy (FTIR), Contact Angle (CA), cell viability assay (using Hela and MCF-7 cancer cell lines as well as non-cancerous human fibroblast cells) by MTT, Bovine Serum Abumin (BSA) protein adsorption using Bradford and bacterial adhesion assay (Staphylococcus aureus and Staphylococcus epidermidis) using microtiter. Results showed that contact angle and surface energey of superhydrophobic modified surface increased to 150° and decreased to 5.51 mj/m2, respectively due to physicochemical modifications of the surface. In addition, the results showed a substantial reduction in protein adsorption and bacterial cell adhesion in superhydrophobic surface.
