Search published articles


Showing 2 results for Tissue Engineering.
Effect of Hydroxyapatite Nanoparticles on Properties of Keratin/Poly Caprolactone Nanofibers for Tissue Engineering
M. Mirhaj, M. Mahmoodi, A. Shybani,
Volume 36, Issue 4 (3-2018)
Abstract
In this research, keratin (Kr)/ poly caprolactone (PCL)/ hydroxyapatite (HA) scaffold was made by electrospinning method. Then, the effect of HA nanoparticles on properties of scaffold B (Kr 33%, PCL 50% and HA 17%) and scaffold A (Kr 40% and PCL 60%) were studied. The surface morphology, functional groups on the surface of samples, porosity, and specific surface area were evaluated by Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectrophotometer (FTIR), liquid displacement method, and BET test, respectively. The mean diameter of fibers in samples A and B was measured 184 nm and 108 nm, respectively. Results showed that the  specific surface area in scaffolds with HA nanoparticles was almost 2 times higher than that of the scaffold without HA. The biodegradability of scaffolds was examined in phosphate buffer solution (PBS) and the results showed an increase in the weight loss percentage of the scaffold B. The cell viability and adherence of osteosarcoma cell line (Saos-2) on the scaffold surface was observed via MTT assay and the results showed an increase in cell growth on PCL/Kr scaffolds with HA nanoparticles. Thus, scaffolds containing HA nanoparticles can be a good choice for tissue engineering applications.

Fabrication of Poly(ε-Caprolactone), Hydrophilic and β-Tricalcium Phosphate Layer- by -Layer Nanofibrous Scaffolds for Tissue Engineering
M. Sohrabi, M. Abbasi, M. M Ansar,
Volume 38, Issue 1 (6-2019)
Abstract
In this study, using biodegradable polymers, nanofiberouse scaffolds were fabricated from the layer-by-layer electrospinning method, including two layer that poly (ε-caprolactone), polyvinylpyrrolidone deposited at first layer and poly (ε-caprolactone), polyvinyl alcohol , β-tricalcium phosphate at latter. After prepration of scaffolds, scanning electron microscopy (SEM), swelling, porosity, mechanical properties and biodegradability behavior in buffered saline phosphate solution were  studied. The results confirmed the bioactivity  and suitable mechanical properties of the layer-by-layer scaffold. The swelling increase with the addition of hydrophilic polymers and reache 811 ± 214 % in the layer-by-layer scaffold, which reveald a significant difference compared to pure PCL. The MTT test on the layer-by-layer scaffold, after 3, 5 and 7 days of rats bone marrow stem cells (rMSC) culture, showed the cell viability of above 80% moreover, cells morphology on the scafold indicated the optimal compatibility of  cells on the scaffold.

Page 1 from 1     

© 2024 CC BY-NC 4.0 | Journal of Advanced Materials in Engineering (Esteghlal)

Designed & Developed by : Yektaweb