Volume 8, Issue 3 (fall 2004)
jwss 2004, 8(3): 45-54 |
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T. Mahmoodi-Ghehsareh, B. E. Sayed-Tabatabaei, C. Ghobadi, A. Mirlohi. A Comparison between Wheat and Maize Cross and Anther Culture Method for Production of Wheat Haploid Plants. jwss 2004; 8 (3) :45-54
URL: http://jstnar.iut.ac.ir/article-1-320-en.html
URL: http://jstnar.iut.ac.ir/article-1-320-en.html
Abstract: (26229 Views)
The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization and anther culture techniques were used for haploid production in six wheat genotypes. The results showed that 70.7% of regenerated plants through anther culture were albino plants and only 29.2 % were green, while the plants produced through wheat × maize method were all green. Ploidy variation was not observed in plants regenerated through wheat × maize hybridization. It was concluded that wheat × maize crosses would be an appropriate and practical method for haploid production in different wheat genotypes, which in comparison with the anther culture method has a higher efficiency.
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