Volume 11, Issue 1 (spring 2007)
jwss 2007, 11(1): 381-391 |
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L. Khodaei, H. Rahimian, R. Amiri, M. Mesbah, A. Mirzaei asl, S. k. Kazemitabar. Identification of RAPD Markers Linked to the Male Sterility Gene in Sugar Beet (Beta vulgaris L.) . jwss 2007; 11 (1) :381-391
URL: http://jstnar.iut.ac.ir/article-1-670-en.html
URL: http://jstnar.iut.ac.ir/article-1-670-en.html
Abstract: (22134 Views)
Genetic male sterility is controlled by one pair of ressesive allele (aa) in sugar beet. This trait is used in most breeding programes. The exsistance of the character in a line or population facilitates transfer of important trait to the breeding material (for example resistance to plant disease). Also, it is possible to increase genetic diversity of monogerm populations by using genetic male sterility. The time and cost of transferring of this gene will be decreased, if the character is tagged with a molecular marker. Bulked segregant analysis using 302 RAPD primers in two F2 populations (231 and 261 population) was performed for the the identification of RAPD markers linked to the genetic male sterility gene. DNA preparation from 8 male fertile and male sterile plants were separately mixed. At first, the primers were tested on bulks. The primers with polymorphic bands were tested on individual plants of the bulks. Only if the polymorphism of the primers was confirmed, they were tested on the other individual plants. Finally, 10 and 6 markers were identified in 231 and 261 populations, respectively, which their distances to male sterility gene were lower than 50 cM. AB-8-18-600r marker was the nearest marker to male sterility gene. This marker showed only 3 and 1 recombination in 231 and 261 populations, respectively. The distance of this marker and genetic male sterility locus was estimated as 5.3 cM in combined F2 populations.
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