Volume 11, Issue 41 (fall 2007)
jwss 2007, 11(41): 373-380 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
S. Esmail Khanian, A. Negati Javaremi, F. Afraz, P. Daneshyar, S. Ghanbari. Genetic Variation among Baluchi Sheep Population Using Microsatellite Markers . jwss 2007; 11 (41) :373-380
URL: http://jstnar.iut.ac.ir/article-1-752-en.html
URL: http://jstnar.iut.ac.ir/article-1-752-en.html
Abstract: (23789 Views)
In order to identify polymorphic microsatellite markers and evaluae genetic variation within Baluchi sheep population, nineteen microsatellite loci were studied. Whole Blood samples were collected from 156 sheep at north eastern animal breeding station of Iran (Abbasabad-Mashhad). DNA was extracted by salting-out procedure with some modifications. Polymerase chain reactions were successfully done except for UNC5C locus. PCR products were electrophoresed on 8% denaturing polyacrylamide gels stained according to rapid silver staining procedure. The genotype and allelic frequencies were calculated by direct counting and used for estimating of different polymorphism and genetic variation criteria. This population wasn't at Hardy-Weinberg equilibrium except for OarAE101 locus (P<0.005). Heterozygosity (gene variation) ranged from 0.1 to 0.93. BULGE5E and BM1329 loci were monomorphic. In conclusion, this investigation showed high polymorphism at the studied loci, so they could be used in future studies.
| Rights and permissions | |
 | This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
