Salinity adversely affects crops metabolism and yield. The present work was conducted to evaluate the singular and interaction influences of Arbuscular mycorrhizal (AM) fungi and brassinolide, as an active group of (brassinosteroids) BRs, on some physiological parameters of wheat plants to cope with salt stress14-day old mycorrhizal (Glomus mosseae) and non- mycorrhizal wheat (Triticum aestivum L.). Plants were foliar sprayed with 0 and 5 µM epibrassinolide 3 times once every two days. Then, each group was treated with 0 and 150 mM NaCl once every 3 days for 10 days. After salt treatment, some plants were harvested to estimate the leaf reducing sugar and glycine betaine contents. After the final growth, all wheat plants were harvested to measure some yield parameters. Synergistic influence of brassinolide and AM fungi was observed in protein and 1000-grain weight. It seemed that this was rooted in the increased accumulation of reducing sugars and glycine betaine, both helping to maintain osmotic potential in cells under high salinity in soil.