JWSS - Journal of Water and Soil Science

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Flax (Linum usitatissimum L.), an oilseed crop, is widely adapted and grown in many regions of the world. Oil from regular flaxseed is used as an industrial drying oil because of the high level of linolenic acid (>50 %). However, the oils from new mutant genotypes of flax with a very low linolenic acid concentration (<2 %) are edible. Yellow seed colour can be used as a visual marker to distinguish edible-oil genotypes of flax from those of industrial type that are usually brown-seeded. In this study, different lines of flax with two seed colours (yellow and brown) in combination with two levels of linolenic acid (high and low) were evaluated in a randomized complete block design for agronomic traits, especially seed yield and its components. The results indicated that lines with high linolenic acid concentration had significantly higher seed yield than those with low linolenic acid. However, other characteristics including those of seed yield components were not siginficantly affected by linolenic acid concentration. Seed colour had a significant effect on number of seedling/m², basal branches, capsules per plant and seed yield per plant. Although seedling emergence was lower in yellow-seeded lines, they had more basal branches, capsules per plant and seed yield per plant. Higher seed yield per plant in yellow-seeded lines can be attributed to higher number of capsules per plant as a result of lower seedling emergence and plant density. Seed yield was not significantly different between brown and yellow-seeded lines. Thus, the effect of lower plant density in yellow-seeded lines was compensated by their higher basal branches and number of capsules per plant.

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A symbiotic relationship exists between the endophytic fungi of the genus Neotyphodium and many cool-season grasses. Endophytes can alter the growth as well as morphological and physiological characteristics of the host plant, thereby influencing the persistence and survival rate of infected plants. This study was conducted to evaluate the effects of endophyte on phenotypic characteristics of Iranian tall fescue (Festuca arundinacea Schreb.) and meadow fescue (Festuca pratensis Huds.). The experiment was set up as a completely randomized design with three replications in a factorial arrangement. The first factor was the two plant accessions and the secondary factor was the endophyte-infected and noninfected plants. The results showed that most of phenotypic characteristics significantly altered as a result of endophyte infection in both tall fescue and meadow fescue plants. Results of analysis of variance showed that the influence of endophyte fungus was positive and significant on tiller number, herbage yield, dry crown weight, dry root weight per plant, and crown depth. Also between plant accessions, there were significant differences for dry herbage yield, crown weight, dry root weight per plant, crown depth, and dry matter percentage. Plant by endophyte interaction was highly significant for crown depth and dry root weight per plant. Therefore, eudophytic fungi can be used to improve phenotypic characteristics in these plants.

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To study the genetic diversity in 100 genotypes of rice, an experiment was conducted at the research farm of Rice Research Institute of Iran. The experimental design was a 10x10 simple lattice. The genotypes, mostly belonging to Isfahan Province and north of Iran, were evaluated on the basis of morphological traits and yield components. The results of analysis of variance demonstrated that the differences among genotypes were highly significant (p < 0/01) for all traits. High values of phenotypic and genotypic coefficients of variation were obtained for most traits, indicating high variability in the traits under study. Factor analysis revealed three factors which determined 90 percent of yeild variation and were named “grain number”, “plant type and structure” and “grain shape”, respectively. Cluster analysis by “Cubic Clustering Criterion” and “Pseudo Hotelling T² Test” grouped genotypes in four clusters. Analysis of variance showed that the differences among clusters were highly significant for most traits.

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To determine the role of endophytes in conferring valuable physiological characteristics on and induction inducing cold tolerance in two species of festuca, an experiment was done at Isfahan University of Technology in 2002. Endophyte-infected and non-infected clones from two genotypes of tall fescue and one meadow fescue were prepared and coded 75,83 and 60 respectively. The clones were exposed to cold treatments at 6, -2 and –10C and compared with control treatment at 20C. After three weeks of cold treatments, relative water content of leaf and crown, cell membrane stability (electrolyte leakage), percentage of membrane damage and finally proline content of leaf were measured. The presence of endophyt caused an slight increase in relative water content of leaf and crown. There was considerably higher proline in endophyte-infected plants compared with non-infected ones in both stress and non-stress conditions. Endophytic fungi had strong effects on maintenance of membrane stability and on the decrease of electrolyte leakage at all temperature levels. Among plant genotypes, 83 preformed better for some characteristics, specially in the presence of endophyte, and showed higher cold tolerance.

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In order to evaluate endophyte effect on plant earliness, compatible host-endophyte combinations including four genotypes of tall fescue and two genotypes of meadow fescue were selected and used in this study. One tiller-part of each genotype was treated using a fungicide mixture of Propiconazole and Folicur. New tillers of endophyte-infected and endophyte-free plants were planted in the field according to a complete block design with three replications. The traits including days to head emergence, days to 50% anthesis, days to maturity initiation, number of heads (as a weekly measurement), total seed weight, net seed weight and empty seed weigh were measured. Results showed that endophytes can enhance head emergence, maturity initiation and periodical seed yield. Days to 50% anthesis, and days to maturity initiation were also reduced. Head numbers in some genotypes increased up to 2 times. Increase in net seed yield may show some physiological alterations in plant such as hormonal balance alteration which may provide better seed setting in infected plants. Based on this study, we concluded that endophytic fungi can induce earliness in infected host plants.

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Seed traits are an important measure of the effects of endophytic fungi in Festuca genus. In this study, endophyte-infected (E⁺) and un-infected (E^-) clones of the same tall fescue genotypes were used to investigate the effects of endophyte on the seed production and associated traits. Endophyte infection resulted in 32.8% increase of total seeds by weight, 34.6% seeds per plant, and 30.6% panicles per plant, but other seed components were not affected by endophyte. Significant phenotypic variances were observed for traits except for 1000 seed weight. Plant genotype×fungal status interactions occurred for seed per plant. These interactions occurred largely because of variation in different plant genotypes. Genetic variance for this trait in E⁺ was greater than E^- for both years, suggesting that the endophyte can mask plant genotypic variance. The results indicated that endophyte had a positive effect on seed production but could result in overestimating of plant genetic variance for some traits. In conclusion, breeding strategies should consider presence or absence of endophyte and the possible effects on variances.