JWSS - Journal of Water and Soil Science

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In the current experiment seven catheterized broiler breeder hens were used at 60 weeks of age. Retention of ¹⁴CO₂ in the body of the hens following continuous infusion of NaH¹⁴CO₃ during each of tow-30-hr continuous infusion studies was determined. Oxidation rate plateau was established within two hours in each experiment. Recovery of ¹⁴CO₂ at the steady state in the first and second study were 91.09 and 87.55 percent respectively and were not significantly different. Mean breath ¹⁴CO₂ recovery was 89.32 percent and hence retention was 10.68 percent. Recovery of ¹⁴CO₂ in breath was not significantly affected by laying of egg or photoperiod. Recovery of ¹⁴CO₂ during the fed state was significantly lower than the fasted state. The results of this experiment indicated that, using of uncorrected data for the retained isotope into body, lead to 10.68 percent AA requirements underestimation in broiler breeder hens by indicator amino acid oxidation technique.