JWSS - Journal of Water and Soil Science

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In an etiological study on stunted, nursery grown maple seedlings, a species of root-lesion nematode (Pratylenchus vulnus) and two species of fusarium (Fusarium oxysporum and F. solani) were isolated from the infected roots. Interactions between the nematode and the two fungi in a complete randomized design with six treatments and four replications were carried out under greenhouse conditions. Nematodes were surface sterilized and reared on sterilized carrot discs. Then the maple seedlings at two-leaf stage were inoculated with a population density of 40 nematodes/100 gr soil in pots. The results indicated singnificant differences between the treatments. Seven months after inoculation, the treatments with nematode alone showed a high mortality rate of about 75% along with a severe increase in population up to about 11.2 and 1266 nematodes/gr of soil and roots, respectively. In treatments with nematode and each of the two-fungus species, the deleterious effects of fungi on nematodes were observed through decrease of nematode population. It was also shown that F. oxysporum was more effective than F. solani in nematode control.

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In order to identify the plant-parasitic nematodes of important cereals (wheat, barley, and corn) in Isfahan Province, 120 soil and root samples were collected from the rhizosphere of these crops in various locations in the region during 1999 and 2000. Nematodes were extracted from soil by sieving and centrifugal sugar-flotation method. The specimens were killed and fixed in FGA (formaldehyde, glycerin and acetic acid 4:1:1) hot solution. The extracted nematodes were mounted in glycerin, permanent slides prepared, and studied by light microscope. The results revealed the presence of three species belonging to two genera of family criconematidae as follows: Criconemella antipolitana , C. xenoplax, Hemicycliophora poranga. H. poranga was extracted from rhizosphere of corn and reported for the first time from Iran. The most frequently occurring and dominant species was C. antipolitana that was isolated from soil around the roots of wheat and barley in several areas. C. xenoplax was extracted from a wheat field with the history of peach cultivation. These species are new records for Isfahan Province.

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Plant parasitic nematodes of Dorylaimida occur only in the Longidoridae. Longidorus is one of important genera in this family, which it,s species are parasite on many plants and act as a vector for some of plant viruses. From 1999 to 2001, more than 250 soil samples were collected from several localities of Iran. Nematodes were extracted, fixed (De Grisse, 1969) and mounted on glass slides in dehydrated glycerine. Using a light microscope equipped with a drawing tube, morphological and anatomical characteristics of nematodes were studied, body parts were drawn, measurements were carried out and the species of nematodes were determined. Species were compared with the original descriptions and variations reported from other regions of the world. Differences and similarities of the species with main description and closely related species were discussed. Three species, Longidorus africanus, L. iranicus and L. pisi were identified the latter species is first record for Iran. L. africanus were mainly occurred in southern regions while L.iranicus were mostly found in northern areas.

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The effect of Rathayibacter tritici on the movement of Anguina tritici larva and nematode function as vector of ear rot bacterium was conducted in the laboratory (Agarose plates) and greenhouse conditions. The results showed that the contact of nematode larva with high concentration of bacterium or long duration of nematode-bacteria contact can decrease the movement and the efficiency of nematode function as the vector of the disease, and in some cases it resulted in the mortality of the nematode. No differences were detected in the mobility of larva in the concentrations less than 10² CFU and less than 0.5 hour of nematode-bacteria contact times and their controls (exposed to water alone). Movement of the nematodes appeared to be random under these conditions. It can be concluded that Rathayibacter tritici did not act as an attractant to Anguina tritici larva. These results suggest that the attachment of a large number of bacteria to nematode (as an essential vector of the bacterium) would induce nematode weakness and mortality. So it is possible that ear rot bacterium can parasite ear cockle nematode, or the nematode is a host for this bacterium .

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To carry out this study, total DNA was extracted from eggs and from second stage juveniles of several populations of Meloidogyne javanica and Meloidogyne incognita, using phenol / chloroform method. Following extraction, DNA was electrophoresed on 1% agarose gel to determine its quality and quantity. A specific primer pair (C₂F₃ / 1108 23 and 20 nucleotides, respectively) was used to discriminate M. javanica from M. incognita populations using polymerase chain reaction (PCR). Primer annealing sites were located in the 3′ portion of mitochondrial gene encoding cytochrome oxidase subunit II (COII) and in the 16S rRNA gene. Following PCR amplification, electrophoresis of amplified DNA showed 1.7 kb fragment in populations of both species. Digestion of 1.7 kb amplified product with HinfI restriction endonuclease resulted in the generation of two DNA fragments of 0.7 and 1.0 kb in M. javanica and three DNA fragments of 0.3, 0.4 and 1.0 kb in M. incognita. There were no differences in the digestion patterns among various populations of each species examined.