Showing 3 results for A. Lakzian
H. Dehghan-Menshadi, M. A. Bahmanyar, S. Salek Gilani, A. Lakzian,
Volume 16, Issue 60 (Summer 2012)
Abstract
Biological indicators are considered soil quality elements, due to their dependence on soil organisms. In order to investigate The effect of compost and vermicompost enriched by chemical fertilizers and manure on soil organic carbon, microbial respiration, and enzymes activity in basil plant's rhizosphere, a field experiment was conducted as a split-plot design with randomized complete blocks and three replications in 2006. The main plot involved six levels of fertilizer including: 20 and 40 tons of compost enriched, 20 and 40 tons of vermicompost enriched per hectare, chemical fertilizer and control without fertilizer and sub-plot, and period of application (two, three and four years). The results showed that application of compost and vermicompost at all levels increased soil organic carbon (OC) and soil microbial respiration, microbial biomass and urease activity compared to the controls (p<0.05), but increasing trend among the treatments was not similar. The maximum amounts of OC, soil microbial respiration and enzyme activity were observed in 40 tons of vermicompost enriched with chemical fertilizer ha-1 with four years of application. In high levels of compost application, the urease activity was decreased.
A. Madani, A. Lakzian, Gh. Haghnia, R. Khorasani ,
Volume 17, Issue 63 (Spring 2013)
Abstract
Mycorrhizal fungus colonization of roots may modify plant metal acquisition. In order to study the role of external hyphae of mycorrhizal fungi in metals transferring, the root and hyphal growth zone were separated by 30 µm nylon meshes. Plants were inoculated with G. mosseae and G. intraradices and grown in the root compartment. Six combinations of metals (400 mg of Zn, 25 mg of Cd, 400 mg of Zn + 25 mg of Cd, 50 mg P, 50 mg of P+ 400 mg of Zn + 25 mg of Cd, and no metal kg-1 soil sample) were added to the hyphal compartments. This experiment was carried out as a completely randomized design (CRD) with factorial arrangement in three replications. The results showed that dry shoot weight and phosphorus concentration in mycorrhizal treatments increased significantly compared with non mycorrhizal treatments. of the two fungal species, G. mosseae had more effect on phosphorus concentration in white clover plant. AM fungi increased the Zn concentration in shoot and root of white clover. AM fungi also significantly increased cadmium concentration in root of mycorrhizal treatments compared to non mycorrhizal plants. of the two fungal species, G. intraradices accumulated more cadmium in clover roots. ALL in all the results demonstrated that AM fungi increased phosphorus and Zn transferring to the shoots while cadmium transferring was decreased by stabilizing in the roots.
H. Rahmani, A. Lakzian, A. R. Karimi Karouyeh, A. Halajnia,
Volume 17, Issue 65 (fall 2013)
Abstract
Urease is one of the most important enzymes in nitrogen cycle. The clay particles (with high surface area) play an important role in the stability of these protein compounds (enzymes) against various environmental factors. In order to examine the interactions between urease with sepiolite and vermiculite, three in vitro experiments were conducted separately in a completely randomized design. Two experiments were carried out with two replications. Treatments included six incubation times (0, 1, 2, 5, 10 and 20 days) and five levels of temperature (10, 20, 30, 40 and 50 ˚C). The third experiment was carried out in a factorial arrangement with two replications. Factors included three levels of pH (5, 7 and 9), and six-levels of enzyme concentrations (0.05, 0.25, 1, 5, 15 and 30 units). The results showed that the activity of adsorbed enzyme was more than free enzyme during the incubation time. The optimum temperatures for activity of free and adsorbed enzymes were 30 and 50 ˚C, respectively. It was concluded that enzyme adsorption on clay surfaces increases enzyme stability against environmental changes. Also, the results showed that the highest levels of urease adsorption on sepiolite and vermiculite occurred at pH 9 and 7, respectively. Adsorption isotherms of Enzyme showed that Vermiculite adsorbed urease with higher affinity compared to Sepiolite.
