Showing 4 results for A. Mousavi
M. Jalali Javaran, H. Hashemzadeh, A. Mousavi,
Volume 8, Issue 2 (summer 2004)
Abstract
Analysis of transgenic plants is very important in gene transfer programs. In this research, the second generation (T1) of transgenic brassica napus which was transformed by antisense of Glutamine synthetase (GS) gene was studied from the view of total soluble protein content of leaf, total chlorophyll and protein patterns (SDS-PAGE) using seeds of Brassica napus .Protein concentration was determined by a calorimetric method described by Bradford method. Chlorophyll (a, b) and carotenoid contents were determined by spectrophotometry. The total soluble protein content of Brassica napus leaves increased from YG stage, reached a maximum level during MG2 and, after this, decreased with the progress of SS stage. Comparison of the total soluble protein between different treatments showed the highest level in the A2 plant and the least in the A6 plant. Comparisons with chlorophyll a and b were not significant between different treatments, but different stages showed significant differences with maximum and minimum levels obtained in the MG1 and SS, respectively. Protein patterns were also studied using SDS-PAGE method. No new band was recognized in the MG1 stage, but the density of some protein patterns was shown in YG and MG2 stages. In YG stage, differences were seen among the A5, A3, A4 and A6 with W.T on 41 KDa weighty position and also between the A1 and A2 with W.T treatment on 23/6 KDa. Considering the molecular weight GS1 subunit of glutamine synthetase (41 KDa) and the similar conditions in growth, protein extraction and macro molecular analysis in transgenic and control plants, we can tell that the difference shown in transgenic plants are probably due to the effect of the antisense of glutamine synthesis gene in this plant.
A. Nasr Esfahani, A. Mousavi Bak,
Volume 8, Issue 3 (fall 2004)
Abstract
The black dot disease of potato is a fungal disease caused by Colletotrichum coccodes (syn. C. atramentarium) that produces accervuli on infected underground plant parts which appear as black dots. The disease appears at the flowering stage and develops up to the season end. The survey on black dot disease of potato in two successive years indicated that the infection percentage is 39.86 in Feraydan (Isfahan), one of the most important potato production regions in Iran. Studies on usual cropping patterns with, potato including, wheat alfa-alfa, barley and fallow showed that in terms of disease infection, one year rotation with fallow reduced the disease severity almost to half compared with control and other cropping patterns. Biological seed and soil treatment, with Trichoderma harzianum in various spores density reduced the disease in various degrees significantly and increased the potato plant growth factors including the number of stems, foliage fresh and dry weights, and also the yields in cases of spore applications, depending upon the type and application dosage. Comparative susceptibility assessments of 24 commercial potato varieties to the black dot disease indicated that the variety Desiree was the least concerned in terms of infection, followed by Scort ,Casser, Casmos, Carlita and Moren , where as Marijke, Cosima and Monalisa were highly infected in comparison to other tested verities and the rests of varieties were scored within these two ranges.
A. Yamchi, F. Rastgar Jazii, C. Ghobadi, A. Mousavi, A. A. Karkhanehee,
Volume 8, Issue 4 (winter 2005)
Abstract
Proline as a key osmoregulating solute in plants plays an overriding role in osmotic pressure adjustment of the cell under water stress conditions. In plant, a bifunctional enzyme delta-1-pyrroline-5-carboxylate synthetase (p5cs) promotes and directs proline synthesis during drought stress conditions. The activity of this enzyme is strongly induced to increase proline concentration within the cell to prevent the impact of water deficit. In this study, the sequence coded for p5cs enzyme under the control of the cauliflower mosaic virus 35S promoter was cloned into a plasmid containing gus and nptII genes. The construct pBI121-p5cs was then transferred into Agrobacterium tumefaciens C58 (pGV3101) and used for producing transgenic tobacco(Nicotiana tabacum cv. Xanthi) plants. The amplification of a 765 bp band within p5cs gene from transferred plants and forming deep blue color in leaf tissues of the explants indicated the successful introduction of p5cs construct into nuclear genome of tobacco plants through Agrobacterium-mediated transformation. The two-month old plants growing under normal condition besides the five-day seedlings under drought stress were subjected to determination of proline concentration. Comparison of P5cs product levels between control and water tolerated plants indicated an increase of proline of about 96.91 to 1330.891 mg/g and of 204.454 to 2039.77 mg/g in plants under normal irrigation and under drought stress, conditions respectively. The significant difference between the levels of proline product in control and transgenic plants under different growing conditions demonstrated the expression of targeted gene (p5cs) in engineered tobacco plants that may pave the way to overcome the water stress problem in agronomically useful crops.
A. Mousavi, F. Khayamim, H. Khademi, H. Shariatmadari,
Volume 18, Issue 67 (Spring 2014)
Abstract
In Iran, no research has yet been performed on potassium release from feldspar in comparison with that from muscovite. The objective of this research was to compare potassium release kinetics of these minerals as influenced by organic and inorganic extractants using successive extraction method. The experiment was carried out in a completely randomized design with a factorial combination. Treatments consisted of three kinds of K-bearing minerals (Muscovite, and Yazd and Ward feldspars), three extractants including CaCl2, oxalic and citric acids of 0.01 M concentration and 6 times of extraction (1, 2, 8, 24 and 48 hours). The results indicated that the potassium release from muscovite was 6-8 times higher than that from feldspars. The type of extractant significantly affected potassium release. Potassium release by organic extractants was 2.5-3 times higher than that by CaCl2 as an inorganic extractant. Different kinetic equations showed that power function, parabolic diffusion and first order equations adequately described K release whereas Elovich equation did not. Among the three equations, power function equation was selected as the best model describing K release from the minerals. Based on the selected kinetic equations, it seems that potassium release from K-bearing minerals is controlled by diffusion process.
