JWSS - Journal of Water and Soil Science

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In order to determine the relationships among the different traits of onion and to study the direct and indirect effects of these traits on bulb and seed yield, an experiment was conducted in 1998 at the research farm of Isfahan University of Technology. Results showed that phenotypic and genotypic correlations were similar and bulb weight showed the highest and lowest coefficients of correlation with bulb diameter and number of days to emergence, respectively. Results of stepwise regression analysis showed that leaf width at 25% of its length from the neck, leaf length, and leaf dry weight were the best estimators for leaf area bulb diameter, bulb length, plant height and number of days to maturity were the most important determining characters for bulb yield variation. Number of fertilized florets was the best determinator of seed yield and bulb weight, while diameter and volume were the best describing characters for the number of meristems on the basal plate. Path-coefficient analysis revealed that bulb diameter showed the highest direct positive effect on bulb yield and the indirect effect of plant height through bulb diameter on it was of prime importance. Number of fertilized florets per plant and number of inflorescence per plant through the number of fertilized florets showed the highest direct and indirect effects on seed weight, respectively.

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This experiment was conducted to study genetic variation for electrophoretic seed protein patterns and their relations with some seed characteristics such as protein and oil percentages, chemical compositions and 100-seed weight among 270 soybean (Glycine max L. Moench) genotypes.

Among different electrophoresis procedures examined, 10% and 4.5% concentrations of acrylamide for resolving and stacking gels, respectively, 13.5 mg/ml concentration of protein buffer extraction, injection of 10 microlitre sample injection into gel hollows, 2.5 miliamper and 2-hours staining period were determined as the best combination to achieve clear bands and good separation. Based on relative mobility on gel, 30 protein bands were observed, of which only 5 varied among genotypes. In general, 8 electrophoretic patterns were recognized. Cluster analysis based on qualitative evaluation of patterns grouped the genotypes in 8 clusters and classified different bands in three groups. Simple concordance (matching) coefficients between protein bands with relative mobilities of 2.5% and 49.5% were zero, which is an indication of their independent occurrence. Probably, these bands are coded with one gene that in dominant and recessive homozygous genotypes appear as a single band at different positions on the gel. Analysis of variance revealed significant differences (P<0.05) among protein patterns for protein and oil percentages. Correlation coefficients between variable protein bands and studied traits showed a positive and significant relation (P<0.05) of bands with relative mobility of 3.5 and 49.5 with protein and phosphorous contents of the seeds, respectively. Protein patterns having band with relative mobility of 49.5 had the highest phosphorous content. Black hilum color of seeds was related to protein band with relative mobility of 52.

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The variation and path coefficient analysis of bread-making quality traits have been studied for 145 genotypes of breeding lines, cultivars and landrace varieties of wheat (Triticum aestivum L.). Bread-making quality traits of genotypes was evaluated indirectly by protein percentage, SDS and Zeleny sedimentation tests, hardness index, test weight, bread volume, grain moisture, and water absorption percentage. Hardness index, Zeleny and SDS sedimentation tests had the highest coefficient of variability (13.51%, 11.83% and 11.03%, respectively). Factor analysis for the genotypes, based on qualitative traits revealed two factors, which explain 98.23% of total variation and were named grain protein index and bread volume factors. The result of correlation analysis indicated positive and significant relationship between protein percentage, SDS sedimentation test and other bread-making quality traits. On the first and second steps of stepwise regression analysis, protein percentage was the most effective trait in explaining different qualitative trait variations. Path analysis also showed the direct and significant effects of protein percentage, Zeleny sedimentation volume, grain moisture and water absorption percentage, and bread volume on SDS sedimentation test. According to cluster analysis based on qualitative traits, the cultivars and landrace varieties, from the view poit of protein quality and quantity, had the highest potential among other groups (generally breading lines).

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Color sticky board traps are used in pest management for capture of adult insect, to estimate and predict population densities in later stages. Yellow sticky board traps showed high attraction for pistachio psylla. It is important to study these relationships for the right placement of traps. For each of three repetition in one year, four pistachio trees were selected randomly in one orchard. Eight traps were located on two heights and four cardinal directions of each tree (32 traps in each repetition). After one week the adult psylla captured were counted. One and two weeks 1ater, eggs and nymph densities were counted on five leaves in one of the eight positions of each tree on four other randomly selected trees (total 160 leaves each time). Data were subjected to analysis of variance. Coefficient of variation was determined between adults captured in each position and eggs and nymph density counts. There were significant differences for adult capture and egg density among directions, and in most cases significant correlations were observed between adults captured on each position and total egg and nymph density count on trees. It was concluded that for egg and nymphal density estimation, we can place traps on either of two heights and four directions except on lower east part, but preferably on either height in south direction of the trees . Also suggestions for egg and nymph sampling are given in the text.

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High attraction is reported for yellow sticky traps to capture pistachio psylla. In pest management, it is important to forecast from the adult population density the density of other developmental stages in order to gain an estimate of the density in damaging stages. In 1998 and 1999, yellow sticky boards with 10150.15 cm were installed in two pistachio orchards in Borkhar district of Isfahan and egg and nymphal densities were simultaneously counted on leaves. Collected data were used to determine regression relationships between two weeks’ average egg density, first and second nymphal instar densities and the sum of egg and first and second instar nymphal densities, one week after the average adult capture in two previous weeks. A low correlation was found between egg count and adult capture, but higher coefficients were obtained between other stages. It was possible to estimate first and second instar nymphal populations from the following equations:

 For high adult densities (X):

Y= 58.6+0.4762X-(7*10^-5)X²                                    R²=0.82                             commercial orchard

 Y= 27.68+0.5092X-(5*10^-5)X²                       R²=0.86                              abandoned orchard

and for low adult densities (X)

Y= 1.7162X-17.454                                     R²=0.97                              commercial orchard

 Y= 1.1117X-4.9841                                    R2=0.90                              abandoned orchard

The application of this method is recommended for the management of pistachio psylla.

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To study the genetic diversity in 100 genotypes of rice, an experiment was conducted at the research farm of Rice Research Institute of Iran. The experimental design was a 10x10 simple lattice. The genotypes, mostly belonging to Isfahan Province and north of Iran, were evaluated on the basis of morphological traits and yield components. The results of analysis of variance demonstrated that the differences among genotypes were highly significant (p < 0/01) for all traits. High values of phenotypic and genotypic coefficients of variation were obtained for most traits, indicating high variability in the traits under study. Factor analysis revealed three factors which determined 90 percent of yeild variation and were named “grain number”, “plant type and structure” and “grain shape”, respectively. Cluster analysis by “Cubic Clustering Criterion” and “Pseudo Hotelling T² Test” grouped genotypes in four clusters. Analysis of variance showed that the differences among clusters were highly significant for most traits.

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An experiment was conducted at Rasht Rice Research Institute, in 2001 to study trait relationships and to determine the direct and indirect effects of different characteristics on grain yield of rice (Orgza sativa L.). A 9x2 factorial experiment in complete randomized block design with 3 replications was used. The two factors were 9 cultivars, namely: Hashemi, Ali Kazemi, Binam, Sepeedroud, Nemat, Khazar, Taichung, Chanto, and Usen, and 2 planting patterns (15x15 cm and 30x30 cm). Significant differences were detected among cultivars, between planting patterns, and their interaction effects. The direct effect of the number of panicles/m² on grain yield was positive and significant in both planting patterns and their combined analysis. Heading date had a positive and significant direct effect on the number of panicles/m² in both planting patterns and their combined analysis. Only in 30x30 cm planting pattern, a positive correlation was found between biomass at heading date and the number of panicles/m². Grain weight per plant had a direct and positive effect on panicle weight in the 3 cases studied. Also, in all the cases studied, flag leaf area had positive direct effects on grain weight per plant. Grain filling rate and effective grain filling period increased in 30x30 cm planting pattern. Native cultivars demonstrated longer latent period. Grain filling rate and effective grain filling period showed positive direct effects on grain yield in both plantings. According to the results obtained, an increase in the number of panicles/m², rate of grain filling, and effective grain filling period would enhance grain yield. Selection for heading date, grain yield per plant, and flag leaf area would also indirectly increase grain yield.

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This study was conducted to analyze the correlation among grain yield, yield components, duration of vegetative and grain filling periods in twelve oat (Avena sativa L.) cultivars at the Research Farm of the College of Agriculture, Isfahan University of Technology, from 2001 to 2003. A randomized complete block design with three replications was used. The correlation coefficients among the grain yield/m², fertile tiller/m², grain number/panicle and the duration of grain filling period were positive and significant. Among yield components, the number of panicle/m² and the number of grain/panicle had the largest direct effects on grain yield (0.68 and 0.30, respectively). Furthermore, the duration of the grain filling period had the highest direct effect on the number of grain/panicle and the grain weight (0.82 and 0.80, respectively), which were greater than its correlations with the number of grain per panicle (0.42) and 1000-grain weight (0.35). However, its negative indirect effects through other traits reduced the correlation coefficients. The results of path analysis indicated that the number of panicle/m² and the number of grain/panicle had the largest direct effects on the grain yield. Considering the direct effects of grain filling duration on the number of grain/panicle and the 1000-grain weight, and also the fact that most of the dry matter in oat is produced in this period, it seems plausible to use these traits as a selection criterion in breeding programs for higher yields of cultivars of oat.

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Diallel analysis was used to estimate the combining ability, gene action, gene number, heritabilties and other genetic parameters of a set of wheat genotypes. For this purpose, nine parents and their 36 crosses were evaluated for 9 traits in a randomized complete block design with three replications in 1996. The analysis of variance revealed significant differences among all genotypes for all traits. Estimates of general and specific combining ability mean squares based on Griffing’s Method 2 indicated the importance of additive and non additive effects in the expression of all traits. Alvand and Roshan cultivars for grain yield per plant, Alvand for grain number per main spike and main spike weight, and Alvand and Alamoot for 1000 grain weight were the best combiners, thus use of them is beneficial for these traits. Based on the estimates of average degree of dominance and results of graphical analysis, the gene action for grain number and spikeletes per main spike were partial dominance, while for grain yield per plant, biological yield, plant height, harvest index and 1000 grain weight, overdominance gene actions were observed. Moreover, genotypic correlation coefficients of grain yield per plant with grain number per main spike, 1000 grain weight, grain weight per main spike and main spike weight were positive and significant.

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Half diallel crosses of nine Iranian wheat cultivars were used to estimate the general and specific combining ability effects and other genetic parameters related to yield and its components. Parents with F2 generations were evaluated at Research Farm, College of Agriculture, University of Shahrekord, in a randomized complete block design with 3 replications. Partitioning of genotypes mean squares to GCA and SCA mean squares based on Griffing’s method 2 and mixed model indicated high significant differences among cultivars in their GCA values for all traits. There were significant differences among crosses in their SCA values for all traits except for flag leaf length, main spike length, plant height and grain yield per plant. The high ratio of GCA to SCA mean squares implied the importance of additive gene effects in the appearance of flag leaf length , main spike length, plant height and grain yield per plant. For flag leaf width, grain number per main spike and peduncle length , the contributions of additive gene effects and for peduncle weight, the contributions of non- additive gene effects were higher. According to the estimates of average degree of dominance, the gene actions for flag leaf length, flag leaf width, plant height, grain yield per plant, main spike length, peduncle length and peduncle weight were partial dominance. The GCA effects indicated that Sefid – Aliabad cultivar was the suitable general combiner for flag leaf length , flag leaf width, plant height , grain yield per plant , main spike length and peduncle length.

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Inheritance of physiologicaly related salt tolerance traits including Na⁺ and K⁺ contents, K⁺/Na⁺ ratio of young leaves and biological yield (BY) in six basic generations (P₁, P₂, F₁, F₂, Bc₁ and Bc₂) and their reciprocal crosses derived from crosses between Kharchia × Niknejad and Shorawaki × Niknejad were studied in sand culture under high salinity treatment (EC = 22.5 dS m^-1). Generation means analysis indicated that a simple genetic model (including additive and dominance effects) is sufficient for Na⁺ and BY in Kharchia × Niknejad cross and for Na⁺, K⁺/Na⁺ ratio and BY in Shorawaki × Niknejad cross but, for other traits digenic interactions (additive × additive and dominance × dominance) were important parameters in the expression of salt tolerance of the various generations. Dominance genetic effects were predominant genetic components in most of the models. Weighted generation variances analysis suggested that dominance variance component was more important for Na⁺ and K⁺ content in both crosses. This result was confirmed by significant differences between back cross generations. Results also showed negligible dominance for K⁺/Na⁺ ratio in both crosses, multidirectional dominance for BY in Kharchia × Niknejad cross and absence of dominance gene action in Shorawaki × Niknejad. Kharchia × Niknejad cross showed, in general, more genetic variation, broad-and narrow sense heritabilities than Shorawaki × Niknejad cross, indicating the important role of primary differences between parents. These results revealed that recurrent selection followed by pedigree breeding or a selective diallel mating system may prove useful in improving salinity tolerance of wheat plants. The involvement of dominance × dominance interactions for some traits indicates that it is necessary to postpone selection for salt tolerance of wheat to advanced generations, when sufficient epistatic interactions have become fixed.