M. Sepehri, N. Saleh Rastin, H. Asadi Rahmani, H. Alikhani,
Volume 10, Issue 1 (spring 2006)
Abstract
Heavy metals have deleterious the effects on nodulation and N2 fixation of Rhizobium- Legume symbiosis, due to their inhibitory effects on the growth and activity of both symbionts. This research has been undertaken to evaluate the effect of Cd tolerance of native rhizobial strains on diminution of the Cd detrimental effects on Sinorhizobium meliloti-alfalfa symbiosis. For this purpose, a greenhouse experiment was conducted based on Randomized Complete Block Design. The treatments in this experiment included: plants inoculation with 6 bacterial strains (sensitive, partially tolerant and tolerant to Cd), 5 levels of Cadmium (0, 2, 5, 10, 20 mg/kg soil) and non-inoculated control. In different levels of Cd, the effects of bacterial inoculation on root nodule number and total amount of nitrogen in plant shoot were compared. The results indicated that soil pollution by Cd even at 2 mg/kg had significant effect on symbiotic properties of rhizobial strains, and according to Cd tolerance of various strains, the mentioned effect was different. Decreasing effect of Cd concentration on root nodules and nitrogen concentration in plants that were inoculated with sensitive strains in comparison with plants inoculated with tolerant strains was 68.31% and 40.8%, respectively. In this research, R95m was introduced as the best strain because of its ability for nodulation and nitrogen fixation.
A. Hemmati, H. Asadi Rahmani,
Volume 22, Issue 4 (Winter 2019)
Abstract
In order to study the effects of rhizobium bacteria and arbuscular mycorrhizal fungi on water use efficiency and the grain yield of bean, under drought stress conditions, two-year year field experiments were conducted during 2015 and 2016 growing seasons. The experimental design was a split plot arranged in an RCBD with 4 replications. Three irrigations including S1= 95-100% AW (Normal irrigation), S2= 75-80% AW(Moderate drought stress) and
S3= 55-60% AW (severe drought stress) were assigned to the main plots and six bio fertilizer treatments including T1=177 rhizobium bacteria strain, T2=160 rhizobium bacteria strain used for seed inoculation, T3= mycorrhizal arboscular fungi used for soil inoculation, T4= T1+T2, T5= T1+T2+T3 and T6= control (no seed and soil inoculation) were randomized to the subplots. Based on the combined analysis of variances for two years, there were significant differences (p˂0.05) in the grain yield, yield components and water use efficiency between the irrigation stress and bio fertilizer treatments. The highest grain yield (2371 kg ha-1) and water use efficiency (522 g m-3) were obtained in the S2T2 treatment. In this treatment, 160 and 177 rhizobium bacteria (T2) in moderate drought stress (S2) were used. These results suggested that inoculation with the rhizobium of seed bean in arid and semi-arid areas could improve yield, water use efficiency and resistance to drought stress by increasing the growth in the root and shoot of the plant.
