Showing 3 results for B. E. Sayed-Tabatabaei
T. Mahmoodi-Ghehsareh, B. E. Sayed-Tabatabaei, C. Ghobadi, A. Mirlohi,
Volume 8, Issue 3 (fall 2004)
Abstract
The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization and anther culture techniques were used for haploid production in six wheat genotypes. The results showed that 70.7% of regenerated plants through anther culture were albino plants and only 29.2 % were green, while the plants produced through wheat × maize method were all green. Ploidy variation was not observed in plants regenerated through wheat × maize hybridization. It was concluded that wheat × maize crosses would be an appropriate and practical method for haploid production in different wheat genotypes, which in comparison with the anther culture method has a higher efficiency.
B. E. Sayed-Tabatabaei,
Volume 8, Issue 4 (winter 2005)
Abstract
Formation of the two- and six-rowed types in barley is predominantly controlled by alleles at a single locus (vrs1) which is located in long arm of chromosome 2H. This gene is a key character on the study of barley domestication and yield. Near-isogenic lines (NILs) of barley were produced from crosses between Kanto Nakate Gold (tow-rowed) and Azumamugi (six-rowed). The selected lines were used for screening of AFLP polymorphic bands which are linked to vrs1 locus. After screening of a total of 1792 primer combination, five polymorphic bands were identified. A construction of high resolution map around the vrs1 locus was made using recombinant inbred lines. These markers can be used for a map-based cloning of the genes at the vrs1 locus.
M. Talebi Bedaf, B. E. Sayed-Tabatabaei, K. Razmjoo, B. Shiran,
Volume 10, Issue 2 (summer 2006)
Abstract
Identification of grass species seems difficult due to the morphological similarities. However, selecting desirable parental genotypes of the crosses based on the genetic distances is considered as the most critical step in a breeding program. The aim of this study was to characterize grass species using AFLP techniques. Five species with five cultivars from each were selected and studied using AFLP reactions performed by PstI and MseI restriction enzymes. The obtained data was analyzed using NT SYS-pc Ver. 2.02 software and Jaccard’s method. Ten primer combinations amplified 1170 bands, all of which were polymorphic between cultivars and species. The maximum band (168) and the minimum number of band (81) were produced by P-AAG & M-CAG and P-ACT & M-CGC, respectively. The results also distinguished 5 species in 40% of genetic distances. Some of the markers were special to some special species that can be used in the identification of that species. Additionally, the results showed that AFLP techniques robust and efficient tools for the identification of genetic relationships of different genotypes within species. High levels of bands and polymorphism make AFLP one of the most powerful markers in the determination and classification of species and different cultivars of grass.
