Sh. Dokhani, R. Beheshti,
Volume 7, Issue 4 (winter 2004)
Abstract
The changes of main sugars and organic acids of Red delicious and Golden delicious apples from Semirom region in Isfahan Province were determined by high performance liquid chromatography (HPLC). The apples were harvested, graded and packed in three kinds of wood, car-board and perforated polyethylene film packages and stored for eight months at 0±0.5oC and 90 to 95% relative humidity for further analysis. Sucarose, glucose and fructose, as well as citric and L-malic acids were detected and quantified in all packed samples. The obtained data were analyzed with factorial experiment in completely randomized design and mean were compared by Duncan,s muliple range test. The results showed that fructose was dominant in both cultivars in all packages compared to other sugars. Golden delicious apples contained more sucrose and less glucose than Red delicious. All sugars decreased in packed apple samples during the storage period. The dominant organic acid of both cultivars was L-malic. Citric acid increased to its maximum level in Red or Golden delicious after one or two months of storage, respectively, and then decreased gradually by the end of storage period. In general, apples kept in perforated polyethylene films exhibited the best physiochemical properties during eight months of cold storage.
A. Sadeghi, F. Shahidi, S.a. Mortazavi, M. N. Mahalati, S.h.r. Beheshti,
Volume 12, Issue 43 (spring 2008)
Abstract
The aim of this study was to use Alpha-amylase termamyl 2-x for maltodextrin production from corn starch and evaluate its industrial uses. Based on the results obtained in laboratory, this process was accomplished in pilot plant scale. The process included preparation of starch suspension, pH adjustment, addition of enzyme, heating under stirring, continuous control of DE and Brix, enzyme inactivation in adequate DE, separation of soluble sections by centrifuge and finally spray-drying of the maltodextrin liquid. In this investigation, the amount of DE at dry matter was calculated under three enzyme concentrations (0.2, 0.25 and 0.3 ml of Alpha-amylase termamyl 2-x per Kg of starch) and in three different hydrolysis temperatures (60, 65 and 70 ºC) at constant pH (6). A completely randomized design with factorial arrangement and 5 replications was conducted. To study the relationship between DE and different parameters, multiple linear regression was used. Finally, for approximation of DE (based on enzyme concentrations, temperatures and hydrolysis times) a regression model was used. The results from different enzyme dosages at the same temperature and time of hydrolysis differed significantly (p≤ 0.05). Meanwhile, the best enzyme concentration and hydrolysis temperature for maltodextrin (high DE) production after 300 minutes were 0.25 ml of enzyme per kg of starch and 70 ºC, respectively. In these conditions, the least residual starch concentration and residual Alpha-amylase activity were observed.
