JWSS - Journal of Water and Soil Science

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The urease enzyme plays an important role in the efficient use of urea fertilizer and some environmental risk assessment. Urease activities in 20 different soil samples of arid to semi-arid regions of Isfahan Province were determined and their correlations with some soil physical, chemical and biological characteristics were studied. Urease activities range from 5.3 to 79.2 µg NH4⁺ g^-1 soil 2hr^-1.

Results indicated that soil organic carbon was significantly correlated with urease activity (r=0.899***). None of sand, silt and clay percentages were significantly correlated with urease activity. Total nitrogen was significantly correlated with urease activity (r=0.797***). Electrical conductivity of saturated paste extracts were also negatively correlated (r=-0.499*) but sodium adsorption ratio (SAR), pH, equivalent calcium carbonate and cation exchange capacity failed to be correlated significantly with urease activity. No significant correlations were found between urease activity and total bacteria (on nutrient agar) or total fungi (on potato dextrose agar), but the bacteria that could colonize urea-agar media were significantly correlated with urease activity (r=0.47*). Multiple stepwise regression analysis showed that organic carbon accounted for most of the variation in urease activity.