Showing 3 results for Esna-Ashari
A. Deljou, O. Karami, M. Esna-Ashari,
Volume 11, Issue 1 (spring 2007)
Abstract
In vitro regeneration of four cultivars of carnation namely ‘Nelson’, ‘Impulse’, ‘Sagres’ and ‘Spitit’ through somatic embryogenesis was studied. MS culture medium was supplemented with 30 gl-1 sucrose, 2 mgl-1 2,4-D and 0.2 mgl-1 BA and used for embryogenic callus formation. Somatic embryos were formed when embryogenic callus was transferred to MS medium without growth regulators containing 30 g/l-1 sucrose alone or supplemented with different concentrations of mannitol (15, 30, 60, 90, 120 and 150 gl-1). No somatic embryo was formed on culture media containing mannitol without sucrose. Number of somatic embryos produced from embryogenic calli significantly increased by adding mannitol to the culture media. Normal embryos formed on culture media containing high concentrations of mannitol (60, 90, 120 and 150 gl-1) developed normally. About 95% of somatic embryos transferred to the1/2 MS culture medium containing 30 gl-1 sucrose, germinated into plantlets. Plantlets also continued their growth under greenhouse conditions.
M. R. Zokaee Khosroshahi , M. Esna-Ashari,
Volume 12, Issue 45 (fall 2008)
Abstract
Effect of different exogenous putrescine concentrations on post-harvest life of strawberry, apricot, peach and sweet cherry fruit was separately studied using completely randomized designs in three replicates. Fruits were immerged in putrescine solutions (0.3, 0.5, 1 and 2 mM for 5 minutes in strawberries, and 0.5, 1, 2, 3 and 4 mM for 10 minutes in the other fruits) and also in distilled water as control. They were then transferred into the fridge. Post-harvest life of all fruits was increased by the use of putrescine. Ethylene production and water loss of the fruits were reduced by this compound. Putrescine also prevented softening of fruit's tissue during their storage period. Titratable acidity was reduced in untreated fruits while their pH was increased. These trends were also observed with the application of putrescine in all fruits, but at a much slower rate compared with the controls. Soluble solids content of sweet cherry fruits was increased by the use of putrescine while it was reduced in the other three fruits.
A. Mahmoodi Pour, M. Esna-Ashari, O. Karami, M. Hesari,
Volume 12, Issue 45 (fall 2008)
Abstract
Resveratrol (5,3,4'- trihyroxystilbene) is a valuable polyphenolic compound which prevents cancer, heart and vascular diseases in human. In this research, the compound was extracted from leaf and fruit tissues of two Iranian grape cultivars (Rajabie Sefide Shiraz and Keshmeshie Ghermez) using a general extraction method with some modification, and analyzed quantitatively by High Performance Liquid Chromatography (HPLC). Resveratrol content in leaf and fruit of Rajabie Sefide Shiraz cultivar was higher than Keshmeshie Ghermez and its amount in the fruits of both cultivars was observed to be greater than their leaves. The accumulation of Resveratrol at the early stage of fruit growth (4 weeks after full bloom) was higher than the subsequent stages of fruit growth and development (8 weeks after full bloom and ripened fruit) in both cultivars. This compound gradually decreased from the beginning of fruit set to the complete stage of growth and development and reached the lowest level when the fruits were ripe. Leaves and fruits of both cultivars were treated with methyl jasmonate (1 mM), then the rate of resveratrol was measured after treatments. It was observed that the amount of this compound was significantly increased in treated samples when compared with the control however, the difference diminished later. The fruits of both cultivars responded better to the treatments 4 weeks after full bloom.
