Showing 3 results for G. Emtiazi
I. Nahvi, M. Vaez, G. Emtiazi,
Volume 4, Issue 3 (fall 2000)
Abstract
Carotenoids are one of the most important and widely distributed pigments in nature and have essential biological functions in organisms. They also impart distinctive and attractive coloration to the breeding animals. Over the past two decades, application of microbial carotenoids has attracted the attention of researchers due to their natural and economic advantages. However, carotenoid producing yeasts with lactose assimilating capacity have rarely been studied and there are few references on the use of lactose as a carbon substrate, such as whey, for the biosynthesis of yeast carotenoids. In this study, a red carotenoid-producing yeast, Rhodotorula acheniorum, was isolated from the sap of birch trees at Masseh Chal in the Taleghan village. The yeast thus isolated was identified through microscopic, macroscopic and biochemical tests.
The yeast has both the abilities of lactose assimilation and carotenoid production. The optimal conditions of culture were determined and maximum biomass and carotenoid production were 9.9 g/lit, 290 µg/g, respectively. Chemical analyses of extracted carotenoids have shown that the major portion of the yeast consists of β-carotene, torulene and torularhodin. With regard to the fact that whey is one of the by-products of cheese industries and that its proper disposal has long been a major problem, the most desirable way of handling this waste is to utilize it as a substrate in the production of useful products such as carotenoids.
A. A. Safari Sinejani, G. Emtiazi, H. Shariatmadari,
Volume 6, Issue 3 (fall 2002)
Abstract
Soil organic matter and clay minerals adsorb and immobilize extracellular enzymes of microorganisms and increase soil enzymes stability. This study aims to clarify the relative importance of soil organic matter and clay minerals on the cellulolytic activities of soils. Fluca prepared cellulase was immobilized on some agricultural residues and clay minerals, avicel and a sample of soil. Immobilized exoglucanase and endoglucanase were assayed at different times. Activities of the immobilized enzymes were strikingly dependent on the kind of sorbent. After 20 days of storing in refrigerator at 4oC, the deactivation of immobilized enzymes on the organic substances (as avicel) was very low but deactivation of immobilized enzymes on the soil and clay minerals was relatively high. On the other hand, the activities of immobilized enzymes on the agricultural residues and avicel were significantly higher than the soil and clay minerals. So it may be concluded that a large part of cellulolytic activity of soil is related to immobilized enzymes on agricultural residues. Coating of the clay minerals, soil and avicel with 4 mmol of Al (OH) x per mg of them significantly increased their immobilization capacity and activities of immobilized enzymes. Activities of immobilized exoglucanase and endoglucanase on the Ca- homoionized soil and clay minerals were significantly higher than the K- homoionized soil and clay minerals. However, these effects may be related to the specific effects of cations on the method of enzyme assay or enzymes activities. These homoionizing-cations effects on the activity of immobilized enzymes on avicel were not significant.
R. Amoo-Aghaie, A. Mostajeran, G. Emtiazi,
Volume 7, Issue 2 (summer 2003)
Abstract
Azospirillum brasilense is one of the N2-fixing microorganisms which, in symbiosis with the roots of cereals and other graminae, enhances growth and development of plants. In this research, wheat seeds (Triticum aestivum) of three cultivars (Ghods, Roshan and Omid) were inoculated with Azospirillum strains (Sp7 and Dol). Inoculation increased growth parameters and yield of wheat cultivars, the effect being directly dependent on the strain-cultivar combination. Strain Sp7 induced the greatest grain yield, 1000-seed weight, number of grains per spike, root and shoot dry weight in Cv. Roshan, whereas strain Dol stimulated the best effect on these growth parameters in Cv. Ghods. The response of Cv. Omid was lower as compared to the other cultivars. Thus, it may be concluded that compatible strains are necessary for increasing yield and enhancing growth and development of wheat cultivars. These experiments also illustrated similar results regarding the effect of strains on N content of seeds. The observation of nitrogenase activity of Azospirillum strains in the In vitro experiments and the significant increases in N content in some inoculated cultivars support the hypothesis that biological nitrogen fixation by Azospirillum could be responsible for the observed beneficial effects on growth parameters. The comparison of nitrogenase activity of two strains showed that acetylene reduction rate for strain Dol was 1.5 times more than that for strain Sp7. Also strain Dol had more pronounced effects on growth parameters, yield and N content of grains than did strain Sp7. Strain Dol is a local strain whereas strain Sp7 is a Brazilian isolate thus, it may be concluded that the local isolates should be preferred to the foreign bacteria, as they are better suited to traditional cultivars, environment and soil conditions of the region.
