JWSS - Journal of Water and Soil Science

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The general characteristics of salt-affected soils and soil structure degradation process are partially known, but the effects of saline and sodic conditions on mechanical properties of soils are not well recognized. In this study, the effects of electrical conductivity (EC) and sodium adsorption ratio (SAR) on tensile strength of soils with different organic carbon contents were assessed under laboratory conditions. The soil samples were collected from Dasht-E-Naz, at Sari region in the North of Iran. The samples had the same clay mineral (Illitic) and the main difference between them was the organic carbon content, subjected to different cropping systems. The tensile strength was determined on soil samples which had been treated by solutions having defined EC (0.5 and 4 dS/m) and SAR (0, 5, 15). The tensile strength was positively related to organic carbon content, but negatively to SAR. With increasing SAR, tensile strength decreased, and at a given SAR, the treatments with higher EC showed higher tensile strength. The analysis of variance showed significant differences (at 0.01) between soil samples (four levels), soil sampling depth (two levels), EC (two levels), and SAR (three levels) for all variables under investigation. For soil factor, the order of averages were: Virgin soil > Permanent pasture (Festuca) > Intensive cropping > Permanent pasture (Agropyron).

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The fire blight with the bacterial causal agent, Erwinia amylovora (Burrill) winslow etal. is one of the most important diseases of the pome fruits that causes the economical losses to quince, pear and apple productions, respectively, in some parts of country. To determine the infection severity of the 43 raturally infected pear cultivars in collection of karaj horticulture research division and also for studing the reaction of these cultivars against disease, the evaluation was performed by the USDA standard system for these cultivars in collection. In this study, although the most of cultivars had been infected on the natural conditions, but disease severity was significantly different between them. By the USDA system, pear cultivars were divided in the 2 classes. Percentage of pear cultivars in very susceptible and moderately susceptible classes were 81.4 and 18.6 respectively. Also for dividing the pear cultivars, beside of USDA method, SPSS software and the cluster analysis were also by the UPGMA method and cultivars were divided into 3 clusters, but it seems that the classification of cluster analysis did not conform with USDA system. Correlation of I.V.S in the artificial tests and the disease severity by natural infection was very significant (r= -0.83).

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This study was conducted in a sugar beet field at Collage of Agriculture Experimental Station, Ferdowsi University of Mashhad, Iran. In order to describe the pattern of spatial variations and density of Chenopodium album, Solanum nigrum, Amaranthus sp., Portulaca oleracea, Echinochla crus-galli, and Convulvulus arvense as the main prevalent annual and perennial weeds of sugar beet fields, geostatistic methods were used. Samples were taken by systematic method from the corners of (7m × 7m) grids, using (0.5m × 0.5m) quadrates in three stages (before application of herbicides, after herbicide treatment, and before harvesting sugar beets). The integrity of spatial variation of variables was determined by using variogram functions and distribution maps of species. The variograms indicated that variations of all variables did not happen by chance. The maximum and minimum ranges of variation were observed in Solanum nigrum (by 142.7m) and Portulaca oleracea (by 1.5m), respectively. Both maximum and minimum ranges of variations were related to pre herbicide application. The highest and the lowest spatial correlations were related to Amaranthus sp. (in the third sampling treatment) and Solanum nigrum (in the first stage of sampling), respectively. The spatial distribution maps confirmed the patchiness distribution of the weeds. The patch of weed was constructed from a dense point at the center, gradually tapering toward the edges. The patches were skewed across the rows and irrigation channels. The structure of patches altered during the growing season. Any information on the distribution of weeds in the fields can be useful to improve decision makings in relation to applying the herbicides, selecting the herbicide type or applying the amount of herbicide. Also it can be useful to better design of weed control programs.

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The present experiment was aimed to evaluate the freezing tolerance of two cold tolerant (MCC426 and MCC252) and a cold susceptible (MCC505) chickpea genotypes. The study was carried out in a split-plot factorial design with three replications. Factorial arrangement of genotype and acclimation (acclimation and non acclimation) were imposed as main plot and temperatures (0, -4, -8, -12, 16, -20ºC) as subplot. The effect of freezing temperature (FT) on plant survival was significantly different among genotypes (p<0.05). According to the average effects of acclimation and FT, the plant survival in MCC426 and MCC252 was 40% and 31% respectively more than in MCC505. Lethal temperature for 50% response (LT50) and temperature resulting in 50% lower dry matter (DMT50) in MCC426 were –10.8ºC and –8.4ºC, respectively and were lower than the other genotypes. Acclimation increased the freezing tolerance such that MCC426 tolerated up to –12ºC without any mortality, however, at this temperature, plant mortality rates in MCC252 and MCC505 were 25.7% and 67.7%, respectively. Plant regrowth was affected by the intensity of FT, such that plant dry weight (PDW) and stem height (SH) in –12ºC decreased about 63% and 50%, respectively, compared with non - frozen control plants. The most freezing damage was observed in MCC505, -12ºC treatment caused 90% decreases in PDW and SH, but at this temperature, PDW and SH in MCC425 decreased 55% and 49% and in MCC252, the reduction was about 60%and 54%, respectively. It seems that the use of controlled experiments would contribute to the evaluation of freezing tolerance and screening programs in chickpea germplasm for the estimation of LT50 and DMT50 .

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The effect of Rathayibacter tritici on the movement of Anguina tritici larva and nematode function as vector of ear rot bacterium was conducted in the laboratory (Agarose plates) and greenhouse conditions. The results showed that the contact of nematode larva with high concentration of bacterium or long duration of nematode-bacteria contact can decrease the movement and the efficiency of nematode function as the vector of the disease, and in some cases it resulted in the mortality of the nematode. No differences were detected in the mobility of larva in the concentrations less than 10² CFU and less than 0.5 hour of nematode-bacteria contact times and their controls (exposed to water alone). Movement of the nematodes appeared to be random under these conditions. It can be concluded that Rathayibacter tritici did not act as an attractant to Anguina tritici larva. These results suggest that the attachment of a large number of bacteria to nematode (as an essential vector of the bacterium) would induce nematode weakness and mortality. So it is possible that ear rot bacterium can parasite ear cockle nematode, or the nematode is a host for this bacterium .

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Genetic male sterility is controlled by one pair of ressesive allele (aa) in sugar beet. This trait is used in most breeding programes. The exsistance of the character in a line or population facilitates transfer of important trait to the breeding material (for example resistance to plant disease). Also, it is possible to increase genetic diversity of monogerm populations by using genetic male sterility. The time and cost of transferring of this gene will be decreased, if the character is tagged with a molecular marker. Bulked segregant analysis using 302 RAPD primers in two F2 populations (231 and 261 population) was performed for the the identification of RAPD markers linked to the genetic male sterility gene. DNA preparation from 8 male fertile and male sterile plants were separately mixed. At first, the primers were tested on bulks. The primers with polymorphic bands were tested on individual plants of the bulks. Only if the polymorphism of the primers was confirmed, they were tested on the other individual plants. Finally, 10 and 6 markers were identified in 231 and 261 populations, respectively, which their distances to male sterility gene were lower than 50 cM. AB-8-18-600r marker was the nearest marker to male sterility gene. This marker showed only 3 and 1 recombination in 231 and 261 populations, respectively. The distance of this marker and genetic male sterility locus was estimated as 5.3 cM in combined F₂ populations.

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A 4x4 factorial experiment with a completely randomized design with three replications was conducted separately on two major pistachio varieties, Ohady and Kaleghoochy, to investigate the effects of shaking amplitude and frequency on pistachio nut and cluster detachment. Four levels of shaking frequencies used for Ohady were 5, 7.5, 10 and 12.5 Hz, while those of Kaleghoochy were 7.5, 10, 12.5 and 15 Hz. Four levels of shaking amplitude for Ohady were 20, 40, 60 and 80 mm, while those of the Kaleghoochy were 40, 60, 80 and 100 mm. Analysis of variance and mean comparison showed that the effects of shaking amplitude and frequency on pistachio nut and cluster detachment were significant on both varieties. It was found that in Ohady, at frequency of 12.5 Hz and amplitude of 80 mm, the maximum effective nut detachment occurred. But considering the need to limit both yield loss and limb damage to 5% and minimizing the power requirement of the limb shaker, applying shaking amplitude of 60 mm and frequency of 10 Hz, which detaches 95% of the nuts, is considered as the most suitable combination of shaking amplitude and frequency. Also, at amplitude of 80 mm and frequency of 12.5 Hz, the highest percentage of cluster detachment occurred. Here again, by considering limb damage and power requirement, applying shaking frequency of 12.5 Hz at 60 mm amplitude with 69% cluster detachment was selected as the most suitable combination. In Kaleghoochi, at amplitude of 100 mm and frequency of 15 Hz, nut detachment was 100%, while the need to limit limb damage and power consumption, the combination of 60 mm amplitude and 15 Hz frequency with 97.2% nut detachment was selected as the most suitable one. For cluster harvesting of Kaleghoochy, shaking amplitudes of 60 and 40 mm and frequency of 12.5 Hz with cluster detachment of 75 and 69 percent, respectively were selected as the most suitable ones. The average ratio of tensile force required for removing pistachio nut to its weight (F/W), and the ratio of tensile force required for removing pistachio cluster to its weight (V/W), which could be considered as an index of ease of detachment, were measured 6.2 and 37 for Ohady and 9 and 37.8 for Kaleghoochy, respectively. Finally, we may conclude that by applying the suitable combination of shaking amplitude and frequency, shake harvesting of pistachio could be practiced successfully.

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In order to detect genetic variation of native fowls in Mazandaran native Fowls breeding station, blood samples were collected from 100 male and female of birds (1:11). The DNA of the blood samples was extracted according to an optimized salting out protocol. The extracted DNA was amplified through polymerase chain reaction (PCR). Of the twenty random primers (10 mer) were used in this study, fourteen yielded satisfactory PCR. The total 63 polymorphic and 77 monomorphic bands were detected for the 14 primers. The number of bands displayed for each primer ranged from 4 to 16 with 200-2100 base pairs. The highest and lowest percentages of polymorphism band were observed for primer 9 (72%) and primer 14 (16%) respectively. The band sharing frequency was calculated for each primer, which ranged from 79 to 96. The genetic similarity within population and genetic variation were estimated as 89 and 11 percentage respectively. In conclusion, the existence of high level of polymorphism after ten generation of selection may indicate the accuracy of genetic evaluation program, suitable selection strategies and also large enough effective population size in this breeding flock.

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In this study, a completely randomized experiment was designed with four irrigation treatments and three replicates. The irrigation programs were raw wastewater, treated wastewater, a combination of 50% raw wastewater and 50% potable water and a combination of 50% treated wastewater and 50% potable water. The experiments were run within a greenhouse. The lysimeters were built up on September 2009 and they were filled with two layers of soil. The upper (0-30 cm in depth) and lower (30-70 cm in depth) layers were sandy loam and sandy clay loam, respectively. A total of eight watering programs with an interval of elevens-day were applied. After each irrigation program, intake wastewater and drainage water of each Lysimeter was sampled in order to analyse the transport of heavy metals (Cu, Zn, Fe and Mn, Ni, Cd and Pb). Results showed that the effect of water quality was significant on percentage of transport of heavy metals. The lowest transport percentage of heavy metals belonged to raw wastewater treatment. Also, the highest percentage of transport of Cu, Zn, Fe, Ni and Pb belonged to the combination of 50% raw wastewater and 50% potable water. In most cases, we observed that the transport percentage of these elements increased by continuing the irrigation