JWSS - Journal of Water and Soil Science

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Twenty-two surface soil samples (0-20 cm) from Fars province were extracted by five extractants (i.e., DTPA, 1.5 M NH₄H2PO₄, 0.1 NH₃PO₄, 0.05 MNa2EDTA, and 1% Na₂EDTA) and used in a 7-week greenhouse experiment which was a 22 × 3 factorial with 22 soils (clay 16-63% pH 7.8-8.2 electrical conductivity 0.32-1.78 dS m^-1 calcium carbonate equivalent 28-63% organic matter 1.1-4.9% cation exchange capacity 9-27 cmol kg^-1 and DTPA-extractable Mn 4-24 mgkg^-1), 3 levels of applied Mn (0, 10, 20 mg Mn kg^-1 as manganese sulfate), 3 replications, and soybean [Glycine max (L.) Merr., cv. Williams]. Application of Mn significantly increased top dry weight by 4 to 104%, averaging to about 12% over the control. But the increase in Mn concentration was not significant. Total Mn uptake also significantly increased but the apparent recovery of the applied Mn was very low (i.e., 0.05%). Soil pH, calcium carbonate equivalent, and clay content were found to be the properties influencing the extractability of Mn. The DTPA-extractable Mn, alone (with a critical level of 13 mg Mn kg^-1) or along with some soil properties, was the only form of Mn that could reasonably predict the plant responses.

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Different Extractants have been proposed to assess available P in different soils. Olsen method is widely used to determine available P in calcareous soils of Iran. However, it has not been compared adequately with other extractants. The main objective of this experiment was to compare this procedure with some other methods. Thirty nine calcareous soil samples from Fars Province with different physico-chemical characteristics were examined for available P by nine extractants. Furthermore, the effects of four P levels (0, 50, 100, 200 µgPg^-1 soil) on growth, P concentration and P uptake by corn were studied in a greenhouse experiment using 20 soil samples. The results indicated that the amount of extractable P decreased in the order, anion-exchange resin > Colwell > Morgan > Olsen > Bray P₁ (1:50) > Soltanpour & Schawb > EDTA > Bray P₁ (1:7) > Water. The amounts of P extracted by all methods, except that by Bray method, showed significant correlation. However, the correlation coefficients between resin, Olsen, Colwell and water were higher than the others. Regression analysis showed that P extracted by all methods, except Bray, was affected by organic matter and native Zn. Moreover, P removed by Bray method was decreased with an increase in calcium carbonate equivalent. Correlation between top dry weight, relative growth and P uptake and P extracted by resin, Colwell, Olsen and water were highly significant. Although the correlation coefficients between growth parameters and P extracted by Morgan and Soltanpour & Schawb were significant, they were of lower magnitude. Plant phosphorus concentration showed significant correlation with P extracted by Olsen and Colwell methods. In general, resin, Olsen and Colwell methods seem to be appropriate extractants for available P in the present study. However, to select the appropriate extractants, more research is needed.

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A study was conducted to determine irrigation scheduling of pinto bean and to evaluate the effect of its planting date. A split-plot with complete randomized block design with four replications was used at Isfahan University of Technology Research Station in Shervedan-Falavarjan, The main plots were three irrigation regimes of T1 to T3 (irrigation after 50±3, 70±3 and 90±3 mm evaporation from class A pan, respectively) and the sub-plots were two planting dates of May 28 and June 28.

The results showed that delayed planting from May to June caused earlier flowering, physiological maturity and reduction of 29.6% in grain yield. Grain yield in T1 to T3 treatments were 3585.1, 3510.5 and 1925.8 kg/ha, respectively. The difference between grain yields of T1 and T2 with T3 treatments were significant at 1% probability level. Biological yields at May 28 and June 28 planting dates were 8257.1 and 5535 kg/ha, respectively, which were significantly different. There was no significant difference between biological yields of T1 and T2 treatments. Number of pods per square meter was the most important component of grain yield and 85% of grain yield difference was due to this part. Harvest index was affected by irrigation treatment and planting date. Mean harvest indices for T1 to T3 treatments were 45.5, 46.1 and 37%, respectively, and 42.8% and 44.9% for the two planting dates. Water use efficiencies for grain yield in T1 to T3 treatments were 0.557, 0.556 and 0.329 kg/m3, respectively. Generally, treatment T2 was shown to be the best irrigation regime.

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As apparent Zn recovery in mineral soils (saturated and unsaturated) is nill, the precise assessment of processes responsible for Zn retention in these soils is of great importance. A laboratory study was conducted to characterize Zn adsorption in eight lowland calcareous soils. The fit of sorption data was evaluated by Freundlich and Langmuir isotherms. In this study, 2-g soil samples were equilibrated for 24 hours with 40 mL 0.0lM CaCl₂ solution containing 5 to 500 mg Zn L^-1. The amount of Zn adsorbed was calculated based on the difference between the initial and equilibrium Zn concentrations.

Zinc adsorption data were fitted to a linear form of Freundlich equation. However the Langmuir isotherm was divided into two distinct linear portions, representing two different types of adsorption sites. The Langmuir K₁ was higher and adsorption maxima (b₁) was lower in part I (corresponding to lower Zn concentration) than in part II (corresponding to higher Zn concentration). Thus, it seems that in parts I and II, sites are more important for their high adsorption energy and adsorption capacity, respectively. Langmuir adsorption maxima (b₂) was positively correlated with clay content, CCE and P concentration and negatively correlated with CEC.

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This experiment was carried out to study the effects of supplemental Cu (0, 100 and 200 mg/kg) and vitamin C (0, 400 and 800 mg/kg) on cholesterol and other blood components in broiler chicks. A total of 360-day-old chicks in a completely randomized block design in a factorial arrangement were used. At 56 days of age one male and one female from each pen were selected, slaughtered and blood samples were collected at slaughtering time for further analysis. After separation of serum, cholesterol, fat components, Cu and vitamin C contents of serum were measured using the relevant methods.

 Copper, vitamin C, cholesterol, LDL and HDL of serum were significantly (P<0.05) affected by vitamin C and Cu supplementation. Hematocrit and mortality were not significantly affected.

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Nitrogen fertilizer application for corn (Zea mays L.) based on available N of soil can decrease the need to N fertilizer and, consequently, reduce the risk of environmental pollution, especially that of groundwater and drinking water. The objectives of this study were i) determining soil N0₃-N critical level at 4 to 6 leaf stage, ii) to study soil nitrate distribution, iii) determining N rate required for maximum corn grain yield, iv) measuring soil residual nitrate nitrogen after harvesting, and v) using hand chlorophyll meter to evaluate N status of corn. The experiment was conducted under field conditions in Bajgah and Kooshkak research stations (Fars Province) in 1996 using a split plot randomized complete block design with four replications. Main plots were control, 60, 120, and 180 kg N ha^-1 as urea. At 4 to 6 leaf stage each main plot was divided into two sub-plots and N was applied at 60 kg N ha^-1 rate to one sub-plot and the other sub-plot was left as control.

 Results showed that critical soil N0₃-N level at 4 to 6 leaf stage in 0-30 cm depth for 90 percent relative yield in Bajgah was 12-14 and for Kooshkak was 8-10 mg kg^-1 soil. The highest coefficient of determination between corn grain yield and soil N0₃-N in 0-30 cm depth for Bajgah was in furrow sample and for Kooshkak was in furrow and shoulder composite sample. In both sites, maximum corn grain yield, with 15.5% moisture content, was about 14 mg ha^-1 that was obtained through the application of 120 kg N ha^-1 as preplant plus 60 kg N ha^-1 as sidedress which is about 1/2 of the application rate used by local farmers. The highest residual soil N0₃-N after harvesting was 24 and 18 and the lowest was 2.6 and 3 mg kg^-1 soil for Bajgah and Kooshkak soils, respectively. When maximum corn grain yield was obtained, chlorophyll meter reading at the middle of leaf blade at dough stage, was 49 for both sites. It seems that N fertilizer application rate by local farmers is excessive.

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Drought can occur at such times when variables such as rainfall depth, run-off, soil moisture, etc. show a deficiency, or variables such as temperature show an increase, or when ground water level shows a decrease in comparison with the average level. Therefore, drought can be evaluated with respect to agricultural, meteorological, or hydrological variability. In this research, considering the meteorological aspects, the method by Herbst et al., later modified by Mohan and Rangacharia, was applied in drought evaluation in Fars Province, Iran. Monthly rainfall measurements over a period of 21 years for 51 stations obtained from Fars Regional Water Board, were used in the analysis. Maps showing lines of iso-duration and iso-intensity lines were developed for the province. Based on the results, northeast, southeast, south, and southwestern parts of the province have the highest potential for being affected by drought events.

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Nitrogen is usually the most limiting nutrient for crop production. Manganese deficiency in some calcareous soils of Iran has been reported. The objective of this study was to evaluate the effect of N and Mn on the growth and chemical composition of spinach (Spinacia oleracea L.) under greenhouse conditions. The experiment was conducted in a soil from Chitgar series (Fine-loamy, carbonatic, thermic, Calcixerollic Xerochrepts). Treatments consisted of factorial arrangement of five N rates (0, 50, 100, 200 and 400 mg kg^-1 as NH₄NO₃) and three Mn rates (0, 15 and 30 mg kg^-1 as MnSO₄) in a completely randomized design with four replications. Plants were allowed to grow for 60 days and a hand-held SPAD-502 chlorophyll meter was used to evaluate leaf chlorophyll status at harvest.

 Results showed that N and Mn application increased shoot dry weight, N and Mn concentrations and total uptake, Mn:Fe ratios and chlorophyll readings, significantly. Nitrogen increased concentrations and total uptake of Zn, Cu and total uptake of Fe in plants, but decreased Fe concentration. Addition of Mn decreased Fe and Zn concentrations in spinach but increased total uptake of Fe and Cu. When maximum dry matter was obtained, the chlorophyll meter reading was about 40. A similar study should be carried out under field conditions before the N and Mn fertilizer recommendations for spinach can be made.

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Micronutrients availability including that of Zn is critical for optimum growth of plants. Zinc availability in calcareous soils of Iran is relatively low due to the presence of a large amount of CaCO₃ and high pH levels. Overapplication of phosphorus to soils may also cause P-induced Zn deficiency. The objective of this experiment was to evaluate the effect of phosphorus and zinc application on the growth and chemical composition of corn (Zea mays L.) under greenhouse conditions. Treatments consisted of a factorial combination of 5 levels of P (0, 25, 50, 100 and 200 µg P/g soil as KH₂PO₄) and 3 levels of Zn (0, 5 and 10 µg Zn/g soil as ZnSO₄. 7H₂O) in a completely randomized design with 3 replications. Plants were allowed to grow for 60 days and then cut at the soil surface.

 Results showed that P and Zn applications increased top dry weight. Applied P increased P concentration and total uptake in plants, but decreased Zn concentration and had no effect on Zn uptake. Zinc application decreased P concentration of com but increased Zn uptake and concentration. The P: Zn ratio in plants increased with P application but decreased with Zn addition. Application of P and Zn increased Fe concentration in plant but decreased Mn concentration and had no effect on Cu concentration. Prior to making any fertilizer recommendations, more research is required to precisely evaluate the response of com to P and Zn applications under field conditions.

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Phosphorus (P) and iron (Fe) are essential nutrients for plants. Iron availability is low in calcareous soils of Iran due to the excessive amounts of CaCO₃ and high pH. Overfertilization of P fertilizers may also decrease Fe availability. The objective of this study was to evaluate the effect of P and Fe on the growth and chemical composition of corn (Zea mays L.) under greenhouse conditions. Treatments consisted of a factorial arrangement of P rates (0, 40, 80, 120 and 160 mg kg-1 as KH₂PO₄) and Fe rates (0, 2.5, 5 and 10 mg kg^-1 as Fe EDDHA) in a completely randomized design with four replications. Plants were grown for 8 weeks in a loamy soil, calssified as Chitgar series (fine-loamy, carbonatic, thermic, Typic Calcixerepts). Results showd that P application up to 80 mg kg^-1 increased corn top dry matter. Corn P concentration and total uptake increased by P application but decreased by Fe application. Application of Fe up to 5 mg kg^-1 increased dry matter but decreased it at higher rates. Concentration and total uptake of Fe increased by Fe application but decreased by P application. Zinc and copper concentrations decresed significantly when P was added. Manganese concentration increased at 40 mg P kg^-1 but decreased at higher rates. Iron application decreased zinc and manganese concentrations but had no effect on copper.

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Iron (Fe) availability is low in calcareous soils of Iran due to high pH levels and presence of excessive amounts of CaCO₃. Overfertilization by phosphorus (P) fertilizers may also decrease Fe availability. The objective of this study was to evaluate the effects of P, Fe and their interactions on the growth and chemical composition of soybean [Glycine max (L.) Merrill] under greenhouse conditions. Treatments consisted of a factorial arrangement of P rates (0, 40, 80, 120 and 160 mg kg^-1 as KH₂PO₄) and Fe rates (0, 2.5, 5 and 10 mg kg^-1 as FeEDDHA) in a completely randomized design with four replications. Plants were grown for 8 weeks in a loamy soil, classified as Chitgar series (fine-loamy, carbonatic, thermic, Typic Calcixerepts). Results showed that P application up to 80 and Fe at 2.5 mg kg^-1 increased shoot dry matter. Phosphorus concentration, total uptake and P:Fe ratio in soybean increased by P application but decreased by Fe application. Application of Fe up to 2.5 mg kg^-1 increased dry matter but decreased it at higher rates. Concentration and total uptake of Fe increased by Fe application but decreased by P application. Interaction of P and Fe had no effect on shoot dry matter. Zinc (Zn) and copper (Cu) concentrations decreased significantly when P was added and manganese (Mn) concentration increased up to 40 mg P kg^-1 but decreased at higher rates. Iron application had no effect on soybean Zn and Cu concentrations but decreased Mn concentration at all rates. Prior to any fertilizer recommendations, it is necessary to study the effects of P, Fe and their interactions on soybean under field conditions.

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Clay minerals have considerable effects on physico-chemical properties of soils. Using different techniques, mineralogy of silicate clays and their formation were investigated in selected soils from Chaharmahal and Bakhtiari Province. The study area is about 1300 ha and its soil moisture and temperature regimes are xeric and mesic, respectively. Geomorphologically, the area consists of different landforms including pediments, outwash, hills and piedmont plain. After field and laboratory studies, five representative pedons were selected in previously determined mapping units and samples were taken for the analyses required. Clay particles from two surrounding rock formations and selected soil samples were separated and examined by electron microscopy (SEM and TEM), X-ray diffraction and infrared techniques. Illite, smectite, chlorite, palygorskite and kaolinite were present in all the soils. Considering the fact that palygorskite was not observed in nearby rock formations, this mineral is probably formed by pedogenic processes. Smectite, on the other hand, has been inherited from parent materials and also transformed from palygorskite. Other minerals had been probably inherited from parent materials. Infrared spectra confirm the X-ray diffraction and submicroscopic results.

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Investigation of paleosols plays a great role in paleoecological and paleoclimatological studies. They are also important in soil survey and planning, as they exhibit characteristics different from younger soils. Paleosols are those soils which formed under conditions different from present ones, and are either buried within sedimentary sequences or those which lie on persisting surfaces. Although such soils are widespread in central Iran and Zagros Zone, they have not been studied adequately. Paleosols are identified by different parameters such as morphological, physical, chemical, mineralogical, and micromorphological characteristics. In this study, morphological, physical, and chemical properties of three paleosols from Isfahan and Chaharmahal & Bakhtiary provinces were investigated. The profiles were on different landforms including alluvial fan, dissected old plain, and old lagoonal deposits. Soil profile in Segzi site, on old lagoonal deposits, had a very dark and thick layer at a depth of 45-60 cm containing some macrofossil shells. This shows that this area was covered by brackish water during the early Holocene. In Sepahanshahr profile, presence of strong clay coating and high concentrations of CaCO₃ indicates a wetter environment in the past than the present conditions with a precipitation of only 100 mm. In Emam-Gheis profile, a buried paleosol was identified with strong clay coating and free CaCO₃ horizons that shows more humid conditions. Evidences obtained from the three paleosols studied indicate that effective moisture in central Iran and Zagros regions during Late Pleistocene had been higher than its present levels.

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Twenty-five surface samples of calcareous soils of Fars Province were used to study the distribution of different nitrogen (N) forms to determine the relationship between the N forms and soil charcteristics, and to obtain regression equations for prediction of N forms from soil characteristics. The forms determined were: soil total nitrogen NO₃-N by phenol disulfunic acid NO₃-N extractable by 2 M KCl NH₄-N extractable by 2 M KCl, 1 N sulfuric acid, and 0.25 N sodium hydroxide oxidative released N by acid permanganate and alkaline permanganate and NH₄-N extractable by 2 M KCl at 100 ^oC. The highest amount of N was that released by alkaline permanganate which constituted 4.47% of soil total N and the lowest form was exchangeable NH₄⁺ which amounted to only 0.6% of total N. Water soluble and exchangeable forms accounted for less than 2% of total N. Highly significant correlations were found between total N and acid permanganate-N (r=0.931) and total N and alkaline permanganate-N (r=0.850). Highly significant regression equations were obtained for prediction of soil total N, acid permanganate-N, and alkaline permanganate-N from soil organic matter (OM), which is an indication of a close relationship of these N forms with OM.

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A factorial experiment with two levels of Zn (0 and 1.5 mg Zn kg^-1), five salinity levels of irrigation water (0, 60, 120 and 180 mM NaC1, and 120 mM NaNO₃) in three replications was conducted. Wheat (Triticum aestivum cv. Roshan) was seeded in pots. After plant harvesting, zinc and cadmium concentrations were determined in the shoot. Activities of metal species in the soil solution were predicted using the computer program MINTEQA2. Treating the soil with NaC1-salinized water increased total concentration of cadmium (Cd_T) as well as Cd²⁺, CdC1⁰₂, and CdCl⁺ species whereas, NaNO₃ treatment had no significant effect on Cd_T. Shoot Cd concentrations were positively related to Cd_T and soil solution Cl^- but negatively related to Zn_T. Application of Zn-fertilizer decreased Cd and increased Zn concentrations in shoot, significantly. The results of this experiment showed that Cl^- has an effective role in increasing mobility of soil Cd and its uptake by plant.

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An experiment was carried out to determine the effect of substituting alfalfa hay (Medicago sativa) with Gundelia tournefortii hay (a range forage in Fars Province) in the ration of fattening lambs. Alfalfa hay was substituted with Gundelia tournefortii harvested at the final stages of flowering at various ratios (0, 25, 50, 75, and 100% dry matter basis) in the ration of fattening lambs. The rations (iso-nitrogenous and iso-energetic) were pelleted and each ration was fed for 75 days to 12 Shirazi Grey ram lambs with an average age of 136 days. The lambs were slaughtered and their feedlot performance and carcass characteristics were measured. Substituting alfalfa hay with Gundelia tournefortii hay did not significantly affect the mean daily weight gain, feed consumption, feed conversion ratio, dressing percentage, internal fat weight, carcass meat content, or meat DM and CP contents (p>0.05). However, backfat depth was significantly different among various rations (p<0.01). At present costs, the inclusion of Gundelia tournefortii in the ration of fattening lambs seemed to be cost-effective the cost of one kg DM of ration was 1266, 1155, 1050, 924, and 787 Rials for rations 1 to 5, respectively.

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The physiological characteristics of winter wheat(cv. Shiraz) were evaluated in a 2-yrs field study by using a spilit plot design with four replications, at the experimental farm of Shiraz University, College of Agriculture located at Badjgah. Main plots consisted of three sowing dates (November 6^th, December 6^th and January 5^th) and four planting densities (150, 250, 350 and 450 plants/m²) were assigned as sub plots. The results indicated that delay in sowing was associated with a significant reduction in the grain yield. So that the grain yield in sowing date of January 5^th was significantly less than it in sowing dates of November 6^th and December 6^th. Moreover with delay in sowing date the developmental rate of wheat was enhanced and plants reached maturity more rapidly. The trend of the leaf area index changes and the dry weight of the plants were under the effect of experimental treatments, leaf area index was found to be greater at the higher densities and early planting date. Number of stems per m² were decreased with delay in the sowing date, although at the higher densities, the number of stems per m² was greater, despite severe tiller death observed at these densities. The study of trend of dry weight changes during the season indicated that biomass was greater at higher densities and earlier sowings, which resulted in earlier ground cover and higher amount of radiation interception. In conclusion, the results of the present investigation revealed that to achieve a reasonable grain yield, the 6^th of December and 250 plants per m² were the best sowing date and planting density for wheat (cv. Shiraz) under similar agro climatic conditions with the present investigation.

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The Effect of various NaCl levels on the shoot content of trace elements, in the seedlings of five citrus species namely: Bakraei (Citrus reteculata X C. limetta), Volkamer lemon(C. volkameriana), Sour orange(C. aurantium), Sweet lime (C. limetta) and Mexican lime(C. aurantifolia), were studied in a glasshouse, by a randomized complete block design with factorial arrangement and four replications. One-year old seedlings of each species were grown in the pots, containing native soil (pH=8.2) and irrigated with water supplemented with 0(control), 20, 40 and 60 mM NaCl. At the end of experiment, the amount of Fe, Zn, Mn, Cu, Cl and B in shoots were determined. The amount of trace elements varied among the species even in the control plants (no salt). Salinity had different effects on the shoot content of trace elements. Salinity reduced Fe content in the shoot of all species except in Bakraii and sweet lime and increased the amount of Zn in the shoot of all species except in Bakraii. The Mn content was reduced in the all species but increased in sour orange. Salinity reduced the amount of Cu in the shoot of volkamerina but had no effect in the other species. The amount of Cl increased in the shoot of all species with salinity. Boron content in the shoot of all species except in sour orange, increased with low salinity level but decreased with increased salinity levels. However in sour orange, salinity in the all levels decreased the amount of B in the shoot of seedlings.

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Fecundity and sex ratio are the important factors in the quality control of parasitoid, Trichogramma. In this research, the effect of the host egg deprivation on these factors in Trichogramma brassicae Bezdenko was evaluated. The experiment was conducted with four treatments, each in five replications in a completely randomized design. Twenty mated females (24 h old) were transferred into the glass tubes (1.5×10 cm) separately and were fed by honey 20% daily. In one treatment, parasitoids were provided with the 150 host eggs (Sitotroga cerealella) in first day, but in other treatments the 150 host eggs were offered after 2, 4 and 6 days. In order to obtain fecundity, black eggs were counted after 3 or 4 days. After emergence, adult parasitoids were recognized as male and female by their antennae and sex ratio was obtained. Results indicated that as parasitoids, Trichogramma sp., were deprived from the host eggs longer, more decrease was observed in fecundity and female ratio. Therefore, parasitoid quality was decreased by the host egg deprivation.

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Paleosols include soils formed under climatic condition different from the present. Although such soils are widespread in central Iran region, adequate investigations of them are yet to be carried out. Micromorphology is one of the most important tools in plaeoclimatological studies. This investigation was carried out to study microscopic features of two paleosols from Isfahan province to reconstruct the paleoclimatic condition during the Quaternary. The results of this study indicate that strong clay coatings are presented in Sepahanshahr paleosol, indicating moisture regime in the past. This paleosol is polygenetic due to calcite and gypsum accumulation during drier periods compared to clay illuviation condition. Micromorphological features in Segzi paleosol indicate that this area has experienced a swampy environment during the younger Dryas. The overall results from this study indicate that climatic oscillation evidences during Quaternary have been preserved in paleosols from Isfahan region.