L. Khodaei, H. Rahimian, R. Amiri, M. Mesbah, A. Mirzaei Asl, S. K. Kazemitabar,
Volume 11, Issue 1 (spring 2007)
Abstract
Genetic male sterility is controlled by one pair of ressesive allele (aa) in sugar beet. This trait is used in most breeding programes. The exsistance of the character in a line or population facilitates transfer of important trait to the breeding material (for example resistance to plant disease). Also, it is possible to increase genetic diversity of monogerm populations by using genetic male sterility. The time and cost of transferring of this gene will be decreased, if the character is tagged with a molecular marker. Bulked segregant analysis using 302 RAPD primers in two F2 populations (231 and 261 population) was performed for the the identification of RAPD markers linked to the genetic male sterility gene. DNA preparation from 8 male fertile and male sterile plants were separately mixed. At first, the primers were tested on bulks. The primers with polymorphic bands were tested on individual plants of the bulks. Only if the polymorphism of the primers was confirmed, they were tested on the other individual plants. Finally, 10 and 6 markers were identified in 231 and 261 populations, respectively, which their distances to male sterility gene were lower than 50 cM. AB-8-18-600r marker was the nearest marker to male sterility gene. This marker showed only 3 and 1 recombination in 231 and 261 populations, respectively. The distance of this marker and genetic male sterility locus was estimated as 5.3 cM in combined F2 populations.
M. Valizadeh, A. Safarnejad, G.h. Nematzadeh, S.k. Kazemitabar,
Volume 11, Issue 42 (winter 2008)
Abstract
Parsi Zira, Bunium persicum (Boiss.) B. Fedtsch., which is called Mountainous or Black Zira, is one of the most important medicinal plants with high economic importance. Generally, there is a little information about in vitro culture of Bunium persicum. Fragmented embryo was used as an explant in Bunium persicum regeneration. In this method, a great callus induction and regeneration only on the same medium without any subculture occurred because of being young and having better interaction with medium, leading to reduction of tissue culture time, infection and chemical consumption. In this research, B5 media containing different concentrations of plant growth regulators, NAA and 2,4-D only or together with Kin, were used. The experiment was conducted using completely randomized design with 30 treatments and 10 replications per treatment. The highest callus number was obtained from the treatment containing 0.1 mg/l 2,4-D and 2 mg/l Kin or 1 mg/l NAA and 2 mg/l Kin. Regeneration occurred in some treatments without Kinetin, showing that Kinetin is not essential for Bunium persicum regeneration. The treatment containing 0.1 mg/l NAA and 4 mg/l Kin was the best one for regeneration. The best treatment for somatic embryogenesis was 2 mg/l 2,4-D.
