Showing 2 results for M. Golabadi
M. Golabadi, A. Arzani,
Volume 6, Issue 3 (fall 2002)
Abstract
To study grain quality traits and their relationships with high molecular weight (HMW) and low moleculor weight (LMW) glutenin subunits, 104 durum wheat genotypes were used. Six grain quality characteristics comprising wet and dry gluten content, test weight, grain hardiness, protein content and SDS sedimentation volume were studied. HMW and LMW glutenin subunits were evaluated using SDS-polyacrylamid gel electrophoresis (SDS-PAGE) in 33 genotypes. Statistical analysis including correlation coefficients, factor analysis, cluster analysis of genotypes based on qualitative traits, analysis of variance in qualitative traits based on HMW, LMW, and combination of subunits as well as all of subunits, and canonical correlation analysis between glutenin subunits and qualitative traits were used.
Factor analysis of the genotypes detected 2 factors, which explained 65 percent of the total variation among the data. These were named quantitative protein and qualitative protein. Based on cluster analysis, the genotypes were classified into four clusters. The genotypes in groups 2 and 4 were beneficial in terms of protein quantity and quality. In evaluating glutenin subunits, 7 HMW subunits and 2 LMW subunits were detected. Subunit null of Glu-Al was observed. Subunits 7+8, 6+8, 13+16 and 20 at Glu-Bl were observed however, neither of them had significant differences in qualitative traits, but 6+8 and 7+8 had a higher SDS sedimentation rate than 20. LMW-1 and LMW-2 had significant differences in protein content and SDS sedimentation LMW-1 had higher protein content whereas LMW-2 had higher SDS sedimentation. Combibation of subunit LMW-1 / HMW 7+8 had the highest protein content and LMW-2/HMW 7+8 had the highest SDS sedimentation. Results of canonical correlation showed that presence of LMW-2 and HMW 7+8, and absence of LMW-1 and HMW 20 in durum wheats caused an increase in SDS sedimentation and a reduction in protein and gluten content.
M. Golabadi, A. Arzani,
Volume 7, Issue 1 (spring 2003)
Abstract
Genetic variation of 300 genotypes of durum wheat comprising CIMMYT/ICARDA and Iranian germplasm was evaluated in 1999-2000 at Researh Farm of College of Agriculture, Isfahan University of Technology located at Lavark, NaJaf-Abad. Days to heading, days to maturity, plant height, spike length, grain weight per spike, number of grain per spike, number of spikes per m2, 1000-grain weight, test weight, grain yield, biological yield, and harvest index were recorded. Coefficients of correlation, step-wise regression, factor analysis and cluster analysis of characters and genotypes were conducted using the studied traits. Results indicated that considerable genetic variations exist for the traits and, in particular for grain yield, harvest index, number of spikes per unit area and number of grain per spike. Grain yield had a positive and significant correlation with days to heading, days to maturity, number of grain per spike and grain weight per spike. Factor analysis for the genotypes detected 6 factors, which explained 76.7 percent of the total variation among data. These factors basically involved potential of assimilate distribution, different aspects of plant storage, source-sink relationships, plant height and tillering potential. Cluster analysis was similar to factor analysis in grouping the characters. According to cluster analysis, the genotypes were calssified into 6 clusters with significant differences among all groups. Mean comparisons of traits in these groups showed that genotypes of groups 5 and 6 were superior in grain yield and harvest index and are beneficial to the local durum breeding objectives.
