Showing 3 results for Mahdikhani
E. Mahdikhani Moghadam, A. Kheiri, M. Mohammadi,
Volume 10, Issue 4 (winter 2007)
Abstract
To carry out this study, total DNA was extracted from eggs and from second stage juveniles of several populations of Meloidogyne javanica and Meloidogyne incognita, using phenol / chloroform method. Following extraction, DNA was electrophoresed on 1% agarose gel to determine its quality and quantity. A specific primer pair (C2F3 / 1108 23 and 20 nucleotides, respectively) was used to discriminate M. javanica from M. incognita populations using polymerase chain reaction (PCR). Primer annealing sites were located in the 3′ portion of mitochondrial gene encoding cytochrome oxidase subunit II (COII) and in the 16S rRNA gene. Following PCR amplification, electrophoresis of amplified DNA showed 1.7 kb fragment in populations of both species. Digestion of 1.7 kb amplified product with HinfI restriction endonuclease resulted in the generation of two DNA fragments of 0.7 and 1.0 kb in M. javanica and three DNA fragments of 0.3, 0.4 and 1.0 kb in M. incognita. There were no differences in the digestion patterns among various populations of each species examined.
H Askarian, B Sharifnabi, M Olia, E Mahdikhani, A Akhavan,
Volume 13, Issue 47 (4-2009)
Abstract
Root knot nematodes (Meloidogyne spp.) cause yield loss in all countries, of which, M. javanica, is the most widespread species in Iran. In order to identify M. javanica, 100 infected root and soil samples of root knot nematode were collected from different regions of Kerman province. After purification of populations and identification of M. javanica based on morphological and morphometerical characters of females and second stage juveniles (J2), total DNA was extracted from eggs, J2 and female adults. Specific 670 and 1600 bp bands were amplified in all M. javanica populations using species-specific primer pairs including OPARjav / OPAFjav and Mjavf / Mjavr These specific bands could not be amplified in other species such as M. incognita and M. arenaria. It seems that, application of these species specific primers in comparison with morphological characters would be more applicable, leading to easier identification of M. javanica.
E Mahdikhani Moghadam, H Rouhani, M Flahi Rastegar,
Volume 13, Issue 48 (7-2009)
Abstract
Sugar beet cyst forming nematode (Heterodera schachtii) is one of the most important pathogens of the sugar beet in Iran. For biological control of Heterodera schachtii, 10 isolates of Trichoderma related to two species T. harzianum and T. virens were examined in laboratory and green house on eggs and cysts for two years. Results obtained from the laboratory assay showed that isolates of Trichoderma parasitized 60% eggs on average. Among them two isolates T. harzianum Bi and T. virens VM1with % 76.18 and %72.55 parasitism, respectively, showed more efficiency compared with the control. In green house, experiments were carried out in autoclaved and non autoclaved soils (field soils) separately with 12 treatments and 3 replications including non infested control (using Ragbi nematicide in field soils experiment), and infested control treated with isolates of Trichoderma using Randomized Complete Design. Then analysis of variance for the bio-control potential of isolates, final population of nematode, fresh and dry root weight, fresh and dry leaves weight inoculated with isolates of Trichoderma was carried out. The results revealed a significant difference (P<0.05) between treatments and control according to Duncan,s Multiple Range Test. T. harzianum Bi and T. virens VM1 decreased population of nematodes, and increased yield in autoclaved and field soils. In autoclaved soils, the two isolates (T. harzianum Bi and T. virens VM1) decreased population of nematodes by %76.68 and %72.65, respectively compared with the control. The Ragbi nematicide, T. harzianum Bi and T. virens VM1 decreased population of nematodes by %81.65, %75.15 and %72.85, respectively compared with the control in field soils experiments.
