Sayed Shahram Mirodjagh, Ahmad Arzani,
Volume 2, Issue 3 (fall 1998)
Abstract
Immature embryo culture response of 28 durum wheat (Triticum turgidum var. durum) cultivars was studied using MS medium in Tissue Culture Laboratory of College of Agriculture, Isfahan University of Technology. Donor plants were grown in a recirculating hydroponic system and pots in a greenhouse. Regenerated immature embryos were recorded at 2, 4, 8 and 16 days after inoculation. Assessment of durum wheat cultivars for immature embryo culture was conducted by percentage and rate of regenerated embryo, using a split plot design arranged in completely randomized design with 5 replications. The analysis of variances showed that there were highly significant differences among cultivars for plant regeneration capacity. The highest potential and rate of embryo regeneration (two days after inoculation) were observed in “Shahivandi” which is a native cultivar of west Iran. “Awl1/Sbl4” with a mean of 48.5%, “Hagla” and “Ofen/Kil” with a mean of 80% have the poorest and the best mean of regeneration during the recording period, respectively. The high regeneration potential is important in view of reducing the time constrain and somaclonal variation in a breeding program.
Sayed Shahram Mirodjagh, Ahmad Arzani,
Volume 3, Issue 1 (spring 1999)
Abstract
In vitro salt tolerance of 28 cultivars of durum wheat with native and exotic origins was investigated, using MS medium containing sodium chloride. The resulting calli were subjected to 8 salinity levels including 0, 0.3, 0.6, 0.9, 1.2, 1.5 and 1.8 percent NaCl (w/v). Donor plants of explant (immature embryo) were grown in a recirculating hydroponics and pots in a greenhouse as well as in the field. Assessment of calli was conducted after 0, 8 and 16 days after subculture of calli into the NaCl-contained medium. Callus growth rate, relative callus growth rate and percentages of callus necrosis were measured, using a 8 × 28 factorial experiment layout in a completely randomized design with 3 observations per treatment and 3 replications. Results of analysis of variances showed that among the criteria, relative growth rate of callus was the most reliable, while callus growth rate as not being quantitatively measured, was less devoted. Results of this study revealed that 'PI 40100' and 'Dipper-6' were superior genotypes for in vitro salt tolerance. The high relative callus growth rate and less callus necrosis of these cvs. may indicate their superior genetic potential for in vitro salinity tolerance.
