M. Jamshid Moghaddam, S. S. Pourdad,
Volume 10, Issue 2 (summer 2006)
Abstract
To evaluate seed germination and seedling growth of safflower in moisture stress, germination of 15 genotypes at four water potential levels (ψ: 0, -0.4, -0.8 and –1.2 Mpa) was tested by Polyethylene glycol (PEG6000). Maximum germination (Gmax), germination rate index (GRI), time length to reach 50% germination (T50), rootlet length (RL) and shootlet length (SL) were investigated. Effects of water potential levels and genotypes were significant for all the characteristics studied. Minimum water potential for germination was estimated between –1.17 and –1.59 Mpa in Goshkhani and LRV-51-51 genotypes, respectively. Different responses to moisture stress were found for rootlet length. In lower potential levels, seedlings had longer, weaker and thiner rootlets in comparison to the zero water potential (control). An increase in stress to –1.2 Mpa led to a severe reduction in rootlet elongation. All stress levels decreased GRI and SL, while potentials lower than –0.4 Mpa decreased Gmax. With a decrease in water potential from 0 to –0.8 Mpa, the T50stage was delayed three-fold compared to the control. Exotic genotypes had longer T50 stage than Iranian genotypes. The highest GRI was related to three Iranian genotypes LRV-51-51, Varamin 295, and Isfahan local, and the lowest was related to CW-74 exotic genotype. Some of genotypes were evaluated for seed yield, oil yield and seed oil percentage under rainfed condition in 2004-05. The highest oil yield average belonged to two exotic genotypes PI- 537598 and Lesaf (326.4 and 313.9 Kg/ha, respectively) and the least was related to Zarqhan 279 Iranian genotype (133.2 Kg/ha). Negative and significant correlation was observed between seed oil percentage and GRI in the 0, -0.8 Mpa potential levels. According to the results, the existing genetic variation for germination parameters can be used for genetic improvement of seed early vigor in safflower.
S.s. Pourdad, K. Alizadeh, R. Azizinegad, A. Shariati, M. Eskandari, M. Khiavi, E. Nabatee,
Volume 12, Issue 45 (fall 2008)
Abstract
Safflower (Carthamus tinctoius L.) is an Iranian native crop that is adapted to different environmental conditions of this country. Sixteen safflower varieties/lines were spring-planted in 6 research stations including Sararood (Kermanshah), Maragheh, Ghamlo (Kordestan), Khodabandeh (Zanjan), Shirvan (North Khorasan)and Khohdasht (Lorestan) with and without drought stress conditions each in a RCBD with 3 replications. Some drought resistante indices including Mean of Productivity (MP), Geometric Mean of Productivity (GMP), Tolerance (TOL), Stress Tolerance Index (STI), Stress Susceptibility Index (SSI) for seed yield and genotypes Cell Membrane Stability (CMS) were calculated. Results showed that STI was the most appropriate index to identify drough resistant genotypes. Estimation of STI from mean of all stations revealed that Gila, CW-4440 and PI-537598 with high STI showed high seed yield in both stress and non-stress conditions. Caculation of STI standard deviation for these genotypes showed that Gila had less STI stability over the locations than other two genotypes so, CW-4440 and PI-537598 are more stable in drought stress resistance. Analysis of variance for cell membrane stability (CMS) of genotypes showed the significant differences in 1% level of probability between genotypes. S-541 had the highest and Kino-76 had the lowest CMS. There were significant and strong correlations between STI, MP and GMP with CMS namely, genotypes with more stable membrane having more drought resistance in field condition. So, cell membrance stability can be a useful and fast method to screen germplasm and identify drought resistant genotypes. Cluster analysis based on STI,MP,GMP, CMS and seed yield in both stress and non-stress conditions divided genotypes into 3 groupes. Results of clustering also identified S-541, Gila, CW-4440 and PI-537598 as 4 superior genotypes and confirmed the results of other methods.
