A. Nekouei, M. Rahimmalek,
Volume 11, Issue 41 (fall 2007)
Abstract
Flora identification of each region plays an important role in the maintenance of natural resources of each country and it is a prerequisite to supplementary phylogenetics and genetic diversity studies. During February 1994 to August 1995, in a two-week interval, vineyards in two districts of Isfahan province, namely Zarinshahr and Tiran & Karvan were visited and weed samples were collected in flowering stages. All collected specimens were identified using specimens present in Herbarium of colleges of agriculture and natural resources of Isfahan University of Technology and other available references. The analysis revealed that in Tiran and Karvan, 84 species belonged to 71 genera and 26 families and in Zarinshahr 62 species belonged to 51 genera and 23 families. Species dominance in both districts belonged to Asteraceae and Gramineae families. Analysis of plant life forms using Raunkiaar method showed that Therophytes with 79.5% and Geophytes with 8.3% were the most frequent life forms in both Zarinshahr and Tiran & Karvan regions.
M. Rahimmalek, B.e. Sayed Tabatabaei, S.a. Mohammadi,
Volume 12, Issue 43 (spring 2008)
Abstract
Genetic maps with high genome coverage are becoming increasingly useful in both basic and applied genetic researches. In the last decades, the advent of DNA markers has brought about a magnificent revolution in the production of genetic map, especially in wheat. In the present study, AFLP markers were used to saturate linkage map of 107 doubled haploid individuals produced through Fukuho _Komugi × Oligo – Culm crosses received from Japan International Research Center of Agricultural Science (JIRCAS). The framework of genetic map was used as base map for next analysis. AFLP analysis was performed with MseI / PstI as digestive enzymes. The average percentage of polymorphism with AFLP markers was around 16.6%. Data analysis was performed by computer program known as Mapmaker / EXP, Ver. 3.3. In this program, the maximum distance criterion was 50 cM and the minimum LOD equated 3. The drawing of chromosome schema for the linkage groups was performed by Draw map, Ver 1.1. In this analysis, 115 AFLP markers were divided into 10 groups in addition, some of the markers remained unlinked. The supplementary data analysis along with specific SSR markers identified the chromosome loci of the markers. Ultimately, 71.1% of the markers were assigned to genome A, 16.5% to genome B and only 3% to genome D. The AFLP markers filled 11 gaps in 7 chromosomes (2A, 3A, 7A, 2B, 3B, 5B and 7B). The low coverage of genome D was due to the limited polymorphism and its conservation in different populations. Among the chromosomes, maximum number of markers (60) was assigned to the chromosome 7A. The distribution of the markers on this chromosome was not uniform. Such a distribution was related to the grouping AFLP markers within heterochromatin region, particularly around the centromere.
