JWSS - Journal of Water and Soil Science

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Pathogenicity and virulence of entomogenous fungus, Verticillium lecanii, was studied on the pea aphid, Acyrthosiphon pisum. Vertalec, a commercial product of V. lecanii, was evaluated under controlled conditions. Second instar nymphs were inoculated with conidial suspensions at concentrations ranging from 10⁴ to 10⁸ conidia/ml, and sterile distilled water (containing wetting agent) as control, then caged on cutted alfalfa stems. Each concentration was replicated three times with 30 aphids treated per replicate. Temperature, relative humidity and photoperiod were maintained at 23±1 °C, %97±3, and 16:8 (L:D), respectively. Aphids were monitored daily over 12 days for mortality and nymph production. All newborn nymphs and dead aphids were removed daily.

Vertalec significantly increased aphid mortality (mean mortality because of mycosis increased form %45.55±6.93 at 10⁴ conidia/ml to %95.55±4.45 at 10⁸ conidia/ml). The LC₅₀ value for pathogen was 5.l4×l0⁴ conidia/ml. LT₅₀ values for 10⁵, 10⁶, 10⁷ and 10⁸ conidia/ml were 10, 8, 6.5 and 5 days, respectively. At 10⁴ conidia/ml LT₅₀ value was not determined at experimental period. R_o values significantly decreased with increasing conidial concentration (mean R₀ altered from 28.l5±5.38 in control to 5.15±1.81 at 10⁸ conidia/ml). Results indicated that vertalec can be an effective agent against pea aphids. Further studies are recommended for its evaluation under natural conditions.

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This manuscript shows the findings of a research that investigated the effects of trainings on the members and the employees of the poultry cooperatives in Tehran Province on the performance of these cooperatives. To analyze the data, 2, correlation coefficient, one way-analysis of variance, and Duncan Test were used. The results of the study showed a positive and statistically significant relationship between training of members and performance of cooperatives. The more the trainings were related to type of occupation or responsibility, the larger were the effects on the cooperative performance. Cooperatives with more specialized top managers, offered more training to their members. Compared to cooperatives with more training, the members had less participation in increasing their investment.

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In order to investigate the androgenic response of chickpea cultivars, two Iranian Chickpea cultivars, Pirooz and Karaj 12-60-31, were used in this study. After 7 to 10 days of cold pretreatment of flower buds, anther containing uninucleate stage of microspores were placed aseptically on MS medium supplemented with various combinations of growth regulators of 2,4-D (1,2 and 3 mg/l) and kinetin (0.1, 0.2 and 0.5 mg/l). Callus regeneration achieved using MS and modified Blayd’s media with various hormones and different sucrose concentrations. The results indicated that the callus initiation was significantly affected by 2,4-D and kinetin concentrations, and that increasing these hormones reduced callus induction. The best response obtained on media with the lowest concentration levels of 2,4-D and kinetin (1 and 0.2 mg/l, respectively). A highly significant genotypic effect and a genotype  2,4-D interaction were detected, which proved that Pirooz response was the best. Callus differentiation and organogensis occurred in MS medium supplmented with NAA, BA and 3% sucrose. Mature embryos also obtained in modified Blayd’s plus 0.5 mg/l kinetin and 10% sucrose. Cytological studies revealed the presence of haploid cells with chromosome variation in the anther derived callus. Therefore, optimizing the hormone levels of different basal media with a particular sucrose cocentration may improve haploid regeneration in chickpea. It seems a further study should be carried out to characterize calli from induction to regeneration and to determine the effect of cold pretreatment, the results of which could be used to improve anther culture response of chickpea.

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Soil organic matter and clay minerals adsorb and immobilize extracellular enzymes of microorganisms and increase soil enzymes stability. This study aims to clarify the relative importance of soil organic matter and clay minerals on the cellulolytic activities of soils. Fluca prepared cellulase was immobilized on some agricultural residues and clay minerals, avicel and a sample of soil. Immobilized exoglucanase and endoglucanase were assayed at different times. Activities of the immobilized enzymes were strikingly dependent on the kind of sorbent. After 20 days of storing in refrigerator at 4^oC, the deactivation of immobilized enzymes on the organic substances (as avicel) was very low but deactivation of immobilized enzymes on the soil and clay minerals was relatively high. On the other hand, the activities of immobilized enzymes on the agricultural residues and avicel were significantly higher than the soil and clay minerals. So it may be concluded that a large part of cellulolytic activity of soil is related to immobilized enzymes on agricultural residues. Coating of the clay minerals, soil and avicel with 4 mmol of Al (OH) x per mg of them significantly increased their immobilization capacity and activities of immobilized enzymes. Activities of immobilized exoglucanase and endoglucanase on the Ca- homoionized soil and clay minerals were significantly higher than the K- homoionized soil and clay minerals. However, these effects may be related to the specific effects of cations on the method of enzyme assay or enzymes activities. These homoionizing-cations effects on the activity of immobilized enzymes on avicel were not significant.

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In this study, susceptibility of the first three larval stages of Spodoptera exigua to Bacillus thuringiensis was investigated. To determine the LC₅₀ values for each larval instar, 6 different logarithmic concentrations of Bacillus thuringiensis were used. A control was also included in each of the bioassays. The value of LC₅₀ for 1st instar larvae, that had been treated one to four hours after hatching, was 311.617 ppm. For the 2^nd and third instars, the larvae values of LC₅₀ were 1356.95 and 2708.27 ppm, respectively. It was clear that larval susceptibility to Bacillus thuringiensis decreased with age. In order to enhance the Bacillus thuringiensis efficiency to control Spodoptera exigua under field conditions, Bacillus thuringiensis should be applied at egg hatch peaks.

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The effect of Bacillus thuringiensis var. kurstaki on different larval instars of Colorado potato beetle, Leptinotarsa decemlineata (Say) and the role of two plant materials, namely, caffeine and aqueous neem extract, in the enhancement of it’s efficiency was investigated under laboratory conditions. The experiments were conducted at 25±4 ^oC, 65±5 %(RH) and photoperiod of 16:8 (L:D).The larvae were provided from the colony established and maintained on host plant. The different larval instars were distinguished by measuring head capsule width.To evaluate susceptibility of different instar groups of larvae to B.thuringiensis, LC₅₀ values on (1-4) instar groups were determined. LC₅₀ values for 1st, 2nd, 3rd and 4th instar groups were, 183.86, 377.03 ,1297 and 3096 ppm, respectively. The synergistic effect of caffeine and aqueous neem extract with B.thuringiensis was investigated separately on 3rd larvae instar. A completely randomized design with 6 treatments and 4 replications was used. The results revealed that there was a striking synergistic effect of caffeine and neem on B.thuringiensis.The larval mortality percentage for mixture 618ppm of B.thuringiensis and 4000ppm of caffeine was 80% however, this was 10% and 20% for caffeine B. thuringiensis respectively when these compounds were used alone.The initiation of larval mortalitry in all mixtures was earlier than other treatments.Also the larval mortality for mixture B. thuringiensis with 35000 ppm of aqueous neem extract was 77.5% after 144 hours however, it was 22.5 % and 25 % for B.t. and neem alone, respectively. The mean weight of larvae treated with all synergists was significantly less than the control cohort, (p<0.05).

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A 2-year vineyard survey failed to the provide evidence that Uncinula necator (Schw.) Burr. survived winter as mycelium in dormant infected buds in Khorassan province. Ascospores of U. necator, were collected by a volumetric spore trap operating constantly in a vineyard for 55 days after the bud burst. The first powdery mildew colonies were consistently found on the leaves of the shoots (7.30 cm long) growing on the vine. Cleistothecia were found on all plant parts infected during the previous growing season. More than 35-45 % of the cleistothecia borne on the leaves and stems died during winter. Most of the ascospore discharge occurred between the bud burst and the blooming time. Ascospores were periodically released from cleistothecia on the leaves kept in vineyard from October to May, while the ascospores germinating on the glass slides germinated from October to January and then germination slowed down to zero in early March and the water content potential of ascospore cytoplasm decreased constantly during this period as well. The mass required to fracture the ascocarp wall during maturation was measured to be approximately 5g in autumn, 3g in winter and 2.5 g in early spring. The most rapid decrease in the strength of the ascocarp wall occurred during a 4-week period before dehiscence in the field. The effect of the pathogenic property ascospores on healthy leaves demonstrated their role as a primary inoculum source. Cleistothecia appear to be the principal means of overwintering of U. necator in Khorassan province vineyards.

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The brown spot disease is one of the seed-borne diseases of rice, found in the all stages of its growth from nursery to farm. It causes qualitative and quantitative damage on rice. Therefore, some studies carried out on the different aspects of this disease and the reaction of some cultivars of rice against the pathogenic fungous agent of the diseases. To do so, 120 isolates, isolated from plant samples were collected from the 91 paddy fields in Guilan. To isolate the pathogenic fungous agent of the disease, the collected samples were cultured on PDA and filter paper. According to the results, the present isolation related to Bipolaris with the 3 species : Bipolaris oryzae (Ito & Kurib.) Drechsler ex Dastur Bipolaris victoriae Nelson Bipolaris sp. The total isolataes were consisted of 15% Bipolaris oryzae, 75% Bipolaris -victoriae and 10% Bipolaris sp. The study of pathogenicity of isolation in these three species was applied to Khazar rice in desicator. The symptoms created by these three species were different from necrotic spots to leaf death. The results indicated that not only the symptoms but also the virulence in these three species were different. The virulence in B. oryzae was more than B. sp and this was more than B. victoriae. To study the reaction of some cultivars of rices to the pathogenic fungous agent, 8 cultivars of rice: “ Bejar, Khazar, Sepeedroud, Domsephid, Hassan saraee, Binam, Neda, Nemat” respectively were used in the two stages of the plant growth, to leaf in the seedling stage and heading. The results indicated that in seedling stage there was no significant difference between these cultivars with respect to the amount of infection. And in heading stage, according to “Dunken” test (5%) they divided into the 3 groups: “Neda, Sepeedrud, Khazar and Binam” with the lowest amount of infection were in the first group, “Bejar, Domsephid and Hassan saraee” classified in the second group and “Nemat” was in the third one. But there was no significant difference between them according to the variance analysis table.

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Drought stress is one of the most important environmental stresses in reduce of growth and plants production. Determination of resistance mechanisms to environmental stress in plant improvement is very important. In order to , experiment with osmotic potentials of PEG (zero (control), -4, -8 & -12 bar) and alfalfa genotypes was done. The selected alfalfa genotypes namely, Yazdi(tolerant), Nikshahri(moderate) and Ranger(sensitive) were grown in hydroponic culture. After 4 weeks, they were harvested in order to determination of proline accumulation and Ca⁺², K⁺ and Na⁺ concentration. The data showed with increasing of drought stress, proline accumulation were increased, but its rate was different among genotypes and organelles. Concentrations of K⁺, Na⁺ and Ca²⁺ increased with increasing of osmotic stress and there was significant different between genotypes. The K⁺/Na⁺ ratio in the shoots and roots of plant was decreased, when drought stress increased. Morphological and biochemical data showed Yazdi genotype was more tolerant to drought stress in compare with studied genotypes.

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The increased potential for soil erosion and compaction due to continuous row crop production and intensive tillage is causing some concern and has led to the consideration of reduced tillage techniques as part of the solution. The objective of this study was to investigate the short-term (one-year) influences of different management practices on the physical properties of a sandy loam soil under corn crop. Treatments were the combinations of three tillage systems (no-till, NT chisel plow, CP and moldboard plow, MP) and three composted cattle manure rates [0, 30 and 60 ton (dry weight) ha^-1]. The experiment was carried out in a split-plot design. Three replicates of the treatments were applied in a randomized block design. Saturated hydraulic conductivity (K_s), total porosity (TP), macro-porosity (Macro-P), micro-porosity (Micro-P) of soil and mean weight diameter (MWD) of aggregates, were measured to a depth of 22.5 cm when 100 percent of the tassels appeared. Tillage and manure combination had significant effects on Log[ K_s], TP, Macro-P and Micro-P. The MP system increased pore space and continuity due to complete inversion and loosening, and as a result K_s, TP, Macro-P and Micro-P were higher than NT system. Higher Macro-P observed for CP might have caused higher K_s versus MP. Reduced tillage systems increased MWD and the increment of manure caused an increase in MWD over all tillage treatments. The results indicate short-term positive effects of manure application on soil pore size characteristics and aggregate stability under moldboard and chisel plowings in the region.

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  Parsi Zira, Bunium persicum (Boiss.) B. Fedtsch., which is called Mountainous or Black Zira, is one of the most important medicinal plants with high economic importance. Generally, there is a little information about in vitro culture of Bunium persicum. Fragmented embryo was used as an explant in Bunium persicum regeneration. In this method, a great callus induction and regeneration only on the same medium without any subculture occurred because of being young and having better interaction with medium, leading to reduction of tissue culture time, infection and chemical consumption. In this research, B5 media containing different concentrations of plant growth regulators, NAA and 2,4-D only or together with Kin, were used. The experiment was conducted using completely randomized design with 30 treatments and 10 replications per treatment. The highest callus number was obtained from the treatment containing 0.1 mg/l 2,4-D and 2 mg/l Kin or 1 mg/l NAA and 2 mg/l Kin. Regeneration occurred in some treatments without Kinetin, showing that Kinetin is not essential for Bunium persicum regeneration. The treatment containing 0.1 mg/l NAA and 4 mg/l Kin was the best one for regeneration. The best treatment for somatic embryogenesis was 2 mg/l 2,4-D.

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In order to diagnose form species of Bipolaris involving root and crown rot of wheat plants and determine their distribution, frequency and disease severity, 410 wheat fields (W. Fs.) in different regions of Kermanshah province were sampled at seedling, tillering and heading stages during three crop years (1997– 2000). Also 90 W. Fs. (30 per year) in different regions of the province were chosen randomly to estimate percentage of infected wheat plants. For the isolation of the fungi from tissues of root, subcrown internodes, crown and foot, the tissues were surface sterilized and placed on culture media. The results showed that there were some root and crown rots in the most regions of the province and the percentage of diseased plants was 0-24%, whose average was 3.2%. Forty three isolates belonging to form genus Bipolaris were isolated including form species B. sorokinina, B. cynodontis and B. spicifera. All three form species were pathogenic under greenhouse conditions. Disease severity and frequency of B. sorokinina were more than the others. Disease severity of B. cynodontis and B. spicifera was the same and their differences compared with the control were significant.

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Transport and filtering of pathogenic bacteria through porous media and groundwater resources are important and، therefore the effect of various factors on bacteria transport and filtering has been given a great attention nowadays. In this study، effects of calcium sulfate and carbonate on Psedomonas fluorescens filtration and filtering parameters were investigated in saturated sand columns under steady-state flow. The calcium carbonate levels included 0، 5، 10 and 20 %w/w and calcium sulfate levels consisted of 0، 5 and 10 % w/w which were thoroughly mixed with sand (0.15-0.25 mm). The experiment was considered factorial in completely randomized design with three replicates. The treated sands were poured into pyrex cylinders with length of 20 cm and diameter of 7 cm. Then، steady-state saturated flow with constant flux was applied to the columns. When the steady-state flow was established، the bacteria suspension with concentration of 10⁶ CFU cm^-3 (C0) was injected as step input into the columns. The leaching then was continued up to 5 times of pore volume (PV). The effluent concentration of the bacteria (C) was measured at 0.25 PV intervals. Then، the sand columns were divided into 0-5، 5-10، 10-15 and 15-20 cm layers in order to measure the filtered bacteria in each layer. The results showed that the effects of calcium carbonate on retaining of the bacteria in the 5-10 and 10-15 cm layers were significant at 1% level. It was significant at 5% for the 15-20 cm layer. The effect of calcium sulfate was also significant at 5% for the 10-15 cm and 15-20 cm layers. The interactive effects of treatments on bacteria adsorption was significant for the 5-10، 10-15 and 15-20 cm layers. The retained concentration profile and the filtration coefficient were significantly affected by the treatments، showing higher bacteria adsorption at lower depths and predominance of physical filtering. The results showed the high filtering capacity of carbonate and sulfate minerals which could ultimately reduce bacteria transport in saturated porous media towards groundwater resources.

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In order to estimate the loss of rice caused by white tip nematode, (Aphelenchoides besseyi), to Alikazemi cv., two series of experiments were conducted in greenhouse and microplot conditions at Rice Research Institute, Rasht (Guilan province) during 2005-2006. The microplots sizes were 0.5×2 meter. Rice seedlings were inoculated by plastic tube method with 0, 100, 300, 500, 700 and 900 nematodes per plant. The experiments were arranged in greenhouse and microplot conditions as a completely randomized design and completely randomized block design with four replications, respectively. The factors under investigation consisted of symptoms (number of plants and Leaves infected), yield and population density of nematodes. The variance analysis of data showed significant differences among treatments in all parameters. The minimum infestation level leading to symptoms and yield loss in microplot condition (500 nematodes) was greater than greenhouse (300 nematodes). Regression analysis of data revealed that the yield reduction was mainly explained by population of nematodes in greenhouse and microplot (R2= 92.56, R2 = 91.55). Also, there was a positive correlation at 5% level of probability between disease incidence and nematode population (R2= 84.42, R2 = 75.27). The resulting equations from regression can be used to estimate crop loss from population of nematodes.

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Determination of suitable species is the most important factor in success of forestation in unfavorable conditions. One of the least costly and the shortest ways for introduction of adaptable species in an area is recognition of the plants that grow naturally. The objective in this study was to find out the effects of protection and water spreading operations on the quantitative improvement of Greek Juniper seedlings. In this study, the areas of water spreading station (Research station of Poshtkoh Water spreading) and an area in western section of water spreading station (an area as control) along the Poshtkoh watershed were chosen. The research was performed on counting of Greek Juniper (Juniper excelsa) in each of two areas. The ratio estimation method was used in a randomized systematic design in strips with the width of 50 meters and 200 meters apart for registration of qualitative parameters of Greek Juniper (Juniper excelsa). The total surface of areas was 600 ha and inventory with intensity of 16 percent has been done. This study showed that in spite of the less number of Greek juniper trees per ha in the station (0/104) in relation to the number of Greek juniper trees per hectare out of the station (0/666), the number of the Greek juniper seedlings in the station was eight times (8.34) more than the number of the Greek juniper seedlings out of the station. As to the effect of protection and water spreading operations on natural growth and increase of number of Greek Juniper seedlings in the station, forestation using this species in this area is recommended.

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In order to study the correlation of agronomic, morphologic and physiologic traits and their effects on grain yield of rice genotypes in two environments (favorable irrigation and water stress), 49 genotypes were evaluated using a completely randomized block design with 3 replications in two experimental conditions. All practices and conditions were the same for the two experiments with the exception of irrigation, where under stress conditions no irrigation was applied at tillering stage. Comparison of means showed significant differences between genotypes in each environment. Also, differences between yield and yield components of each genotype under two conditions were significant. The results of phenotypic correlations showed that the highest positive and significant correlation with grain yield belonged to number of panicle per plant (0.95) in irrigation conditions and to number of filled grains per panicle (0.92) in water stress conditions. Stepwise regression analysis for grain yield introduced number of panicle per plant, relative water content (RWC), flag leaf length and number of spikelet per panicle, respectively, as effective traits in grain yield in irrigation conditions, however, in stress conditions, number of filled grain per panicle, number of panicle per plant and relative water content were effective traits in yield. The results of path analysis showed that the number of panicle per plant had the highest positive and direct effect on grain yield in the two environments. Factor analysis introduced four factors in the two conditions named yield and crop production, phenologic, harvest index and plant shape and appearance quality of grains factors. Therefore, to select high yield and drought tolerant genotypes, we need to consider number of filled grain per panicle, number of panicle per plant and relative water content. In addition, traits such as panicle length, number of spikelet per panicle, flag leaf length and width that showed significant correlations with grain yield in stress conditions should also be considered important and second to the above mentioned traits.

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In this study, the transport of nalidixic acid-resistant Escherichia coli (E. coli NAR) through two soils of sandy loam and clay loam was investigated. Saturated and unsaturated flow conditions were applied at two temperatures of 5 and 20ºC. Leaching was done using large repaired soil columns which had been subjected to physical weathering. A 20-cm diameter disk infiltrometer was set up to establish the steady-state flow conditions. Effluent was sampled at three depths of 15, 30 and 45 cm of soil columns. Saturated flow condition, temperature of 20 ºC and clay loam soil resulted in increasing the bacteria concentration in the leachate. Filtration coefficient and relative adsorption indices in sandy loam soil (average flow conditions, temperature and depth) were greater than those of clay loam soil with the respective values of 33% and 23%. These results may be related to the instability of soil structure and abundance of micropores in the sandy loam columns. In other words, the bacteria were physically blocked and entrapped in the fine pores of sandy loam soil. Effluent bacteria concentration decreased by depth of soil column, indicating the effect of soil on bacterial filtration as a natural filter. Leaching with cold water led to decrement of flow rate and consequently increment of bacterial filtration in the two soils of clay loam and sandy loam (average flow conditions, temperature and depth) with the respective values of 100% and 68%.

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The effects of soil intrinsic properties on soil structural stability were evaluated. Soil samples (33 series) with wide ranges of properties and structural stability were collected from Hamadan province. Two structural stability indices were used: mean weight diameter (MWD) using Yoder method and De Leenheer-De Boodt index (DDI). Wetting pre-treatments (fast wetting to saturation and slow wetting to a matric suction of 30 kPa) were applied before wetting. Linear and multiple regression relations of MWD and DDI with the soil intrinsic properties (organic matter, clay, fine clay, silt, sand, calcium carbonate, EC and pH) were assessed. Results showed that organic matter had the highest impact on the two mentioned indices. Following organic matter, clay, fine clay and calcium carbonate were ranked respectively one after another. Fast wetting caused a higher aggregate break-down, due to its destructive energy, air entrapment, and non-uniform swelling of the soil whereas slow wetting exhibited better differentiation of soils with low structural stability. The findings of this research demonstrated high agreement (R2>75%) between the MWD and DDI, recommended both to be used for evaluating of the aggregate stability in Hamedan province

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In agriculture, cow manures are used to enhance soil fertility and productivity. Escherichia coli is the most common fecal coliform in cow manure and considered as an index for microbial contamination of groundwater resources. The objective of this study was to investigate the transport of Escherichia coli (released from cow manure) through the field soil. Lysimeters (with internal diameter of 20.5 and height of 50 cm) were inserted into an in situ clay loam soil. Unsaturated soil water flow was controlled at an inlet matric potential of –5 cm using a tension infiltrometer. When the steady-state flow was established, air-dried fresh cow manure was applied on the lysimeters at a rate of 10 Mg ha-1 (dry basis) and the soil-manure leaching started. Soil solution was sampled at 1, 2, 4, 6, 12 and 24 h after leaching initiation using plastic samplers installed at depths of 20 and 40 cm. Concentrations of Escherichia coli in the soil solution (C) and the influent (C0) were measured using the plate count method. Impacts of soil depth, sampling time, and their interaction on C and C/C0 were significant (P<0.01). In all leaching times, relative adsorption index (SR) was lower when both soil layers were considered and the filtration increased with soil depth. When the concentration was corrected for the second layer (i.e. 20–40 cm), the SR values in this layer were considerable and greater than those in the first layer at 4 and 6 h. The influence of surface layer was substantial in bacterial filtration however, the preferential flows especially in the initial leaching times resulted in bacterial movement towards the second layer. Temperature drop reduced bacteria release from the manure, increased viscosity of the flowing water, and consequently diminished significantly the bacteria concentration in the soil solution at 24 h. Overall, it was found that similar to surface layer, subsurface layer might have great role in bacterial filtration due to its higher clay and carbonate contents

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In this study, a completely randomized experiment was designed with four irrigation treatments and three replicates. The irrigation programs were raw wastewater, treated wastewater, a combination of 50% raw wastewater and 50% potable water and a combination of 50% treated wastewater and 50% potable water. The experiments were run within a greenhouse. The lysimeters were built up on September 2009 and they were filled with two layers of soil. The upper (0-30 cm in depth) and lower (30-70 cm in depth) layers were sandy loam and sandy clay loam, respectively. A total of eight watering programs with an interval of elevens-day were applied. After each irrigation program, intake wastewater and drainage water of each Lysimeter was sampled in order to analyse the transport of heavy metals (Cu, Zn, Fe and Mn, Ni, Cd and Pb). Results showed that the effect of water quality was significant on percentage of transport of heavy metals. The lowest transport percentage of heavy metals belonged to raw wastewater treatment. Also, the highest percentage of transport of Cu, Zn, Fe, Ni and Pb belonged to the combination of 50% raw wastewater and 50% potable water. In most cases, we observed that the transport percentage of these elements increased by continuing the irrigation