Showing 5 results for Sayed-Tabatabaei
T. Mahmoodi-Ghehsareh, B. E. Sayed-Tabatabaei, C. Ghobadi, A. Mirlohi,
Volume 8, Issue 3 (fall 2004)
Abstract
The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization and anther culture techniques were used for haploid production in six wheat genotypes. The results showed that 70.7% of regenerated plants through anther culture were albino plants and only 29.2 % were green, while the plants produced through wheat × maize method were all green. Ploidy variation was not observed in plants regenerated through wheat × maize hybridization. It was concluded that wheat × maize crosses would be an appropriate and practical method for haploid production in different wheat genotypes, which in comparison with the anther culture method has a higher efficiency.
B. E. Sayed-Tabatabaei,
Volume 8, Issue 4 (winter 2005)
Abstract
Formation of the two- and six-rowed types in barley is predominantly controlled by alleles at a single locus (vrs1) which is located in long arm of chromosome 2H. This gene is a key character on the study of barley domestication and yield. Near-isogenic lines (NILs) of barley were produced from crosses between Kanto Nakate Gold (tow-rowed) and Azumamugi (six-rowed). The selected lines were used for screening of AFLP polymorphic bands which are linked to vrs1 locus. After screening of a total of 1792 primer combination, five polymorphic bands were identified. A construction of high resolution map around the vrs1 locus was made using recombinant inbred lines. These markers can be used for a map-based cloning of the genes at the vrs1 locus.
M. Talebi Bedaf, B. E. Sayed-Tabatabaei, K. Razmjoo, B. Shiran,
Volume 10, Issue 2 (summer 2006)
Abstract
Identification of grass species seems difficult due to the morphological similarities. However, selecting desirable parental genotypes of the crosses based on the genetic distances is considered as the most critical step in a breeding program. The aim of this study was to characterize grass species using AFLP techniques. Five species with five cultivars from each were selected and studied using AFLP reactions performed by PstI and MseI restriction enzymes. The obtained data was analyzed using NT SYS-pc Ver. 2.02 software and Jaccard’s method. Ten primer combinations amplified 1170 bands, all of which were polymorphic between cultivars and species. The maximum band (168) and the minimum number of band (81) were produced by P-AAG & M-CAG and P-ACT & M-CGC, respectively. The results also distinguished 5 species in 40% of genetic distances. Some of the markers were special to some special species that can be used in the identification of that species. Additionally, the results showed that AFLP techniques robust and efficient tools for the identification of genetic relationships of different genotypes within species. High levels of bands and polymorphism make AFLP one of the most powerful markers in the determination and classification of species and different cultivars of grass.
B. Heidar, G.h. Saeidi, B.e. Sayed-Tabatabaei,
Volume 11, Issue 42 (winter 2008)
Abstract
In this study, factor analysis was conducted to determine the factors which contributed to the variation of quantitative traits and path analysis was performed to find the direct and indirect effects of yield components on grain yield in bread wheat. A doubled haploid population of 157 lines of wheat (Triticum aestivum L.) was evaluated for agronomic and morphological traits, using a randomized complete block design with three replications in 2003 and 2004. The results of factor analysis based on maximum likelihood indicated five factors explaining 80.4% and 73.9% of total variation in 2003 and 2004, respectively. The first factor in 2003 had 30.5% contribution to the total variation, strongly influenced by the traits of pollination date, heading date, flag leaf length and days to maturity. This factor also indicated the negative relationship among the yield components and the importance of relationship between grain yield and some morphological traits. The first factor in 2004 was more affected by grain weight/spike, grains/spike and 1000-grain weight, thus it was named as grain yield factor. The second and third factors in 2003 were considered as plant height and grain yield and in 2004 as maturity and plant height, respectively. The results of path analysis showed that grains/spike had the most direct and positive effects on grain yield in 2003 (1.33) and 2004 (0.87). Because of the negative and high indirect effects of grains/spike via fertile spikes/m2 and 1000-grains weight on grain yield, the correlation coefficient between grain yield and grains/spike was very low. There was not much difference between the phenotypic and genetic direct effects of spike/m2 on grain yield, indicating that their relationship was less affected by environmental conditions. In general, the results showed that grains/spike and spikes/m2 can be more efficient compared to 1000- grains weight for increasing grain yield and can be used as selection indices in breeding programs. Also, according to the results of factor analysis, selection based on the fourth factor including biological yield, spike/m2 and grain yield as selection index can be effective to improve grain yield in breeding programs.
C. Ghobadi, M. Khosh-Khui, B.e. Sayed-Tabatabaei,
Volume 12, Issue 45 (fall 2008)
Abstract
Grapevine (Vitis vinifera L.) is a clonally propagated major fruit crop. In grapevine, identification of genotypes with amplographical features is often based on mature plant characteristics that may be affected by environmental conditions. This approach lacks objectivity and reliability. Recently, molecular markers have proved to be supplementary techniques to analyze genetic diversity and examine genetic relationships existing between cultivars in a range of horticultural crops. In this study, twenty genotypes from grapevine (V.vinifera species) grown in Isfahan province were characterized by RAPD technique to understand the extent of diversity and relatedness. Fifty random primers were used for the RAPD study. Of those, twenty four informative primers which generated reproducible polymorphic bands were used for grouping the genotypes. PCR products of the genotypes’genome revealed a total of 315 bands, out of which 282 were found to be polymorphic. Average number of 13 bands was obtained per primer and the amplification produced ranged in size from 300 bp to 3000 bp. The dendrogram constructed using UPGMA cluster analysis differentiated the genotypes into two major clusters, nineteen in one group and Madar-o-Bache genotype has been placed in a separate one, indicating its high genetic diversity compared to the rest of the genotypes. Intra-clustering within cluster A grouped the genotypes in four sub-clusters as expected from their genetic background. The results of the study revealed that the RAPD technique is a relevant technique to determine genetic diversity, genomic analysis and to examine genetic relationship in grapevines.
