Showing 3 results for Sharifnabi
Bahram Sharifnabi, Asghar Nekoei,
Volume 1, Issue 2 (fall 1997)
Abstract
In order to study fungi associated with seeds of sainfoin, several seed samples of sainfoin were collected from Isfahan, Ardabil, Zanjan and eastern Azerbaijan provinces, Iran. Seed lots, only half of which were surface sterilized, were allowed to grow on PDA, SMA, MA, sterilized wet paper and drenched sands. The dishes containing seeds were incubated at 25°C with alternate cycles of 12-hour fluorescent light. After seven days, fungi growing from the seeds were transferred to the selective media and subjected to morphological studies. Single spore or hyphal tips from these transfers were the basis for all identification procedures. The most prevalent fungi associated with sainfoin seed were Alternaria, Aspergillus, Penicillium and Rhizopus, although Ulocladium, Cladosporium, Fusarium, Mucor, Nigrospora, Stemphylium, Trichothecium and Botrytis were also isolated in low incidence from these seed lots. The isolates of Uromyces and Oidiopsis were also obtained when the seed samples were examined by washing method.
B. Sharifnabi, G. Saeidi,
Volume 8, Issue 3 (fall 2004)
Abstract
Safflower (Carthamus tinctorius L.) is one of the multi-purpose oilseed crops which has a high adaptation to different conditions such as resistance to drought and it is suited to be grown in arid and semi-arid regions such as Isfahan province. Root rot disease is an important soil-borne disease of safflower in Isfahan, which can be caused by different pathogens. The objective of this study was to determine the causal agent of safflower root rot and to evaluate different genotypes for tolerance to the disease. Different species of Fusarium were isolated from sample collections. Laboratory and greenhouse inoculations indicated that F. solani was the only pathogenic species. In this experiment, 60 genotypes of safflower including breeding lines selected from various Iranian local populations and foreign cultivars were evaluated for reaction to the disease in a randomized complete block design with three replications in greenhouse. Artificial inoculation via injection of spore suspension of F. solani (106 spores/ml) was conducted on 8-week plants and then development of necrosis and death percentage were recorded. The results showed that there were significant differences among the genotypes in terms of reaction to the disease. The most resistant and susceptible genotypes were breeding lines of IUTE14310 and IUTC121 with mean necrosis of 9.67 and 28.33 mm, and death percentage of 32 and 74, respectively. Based on the means of necrosis and death percentage, the genotypes were significantly classified in 5 distinct groups including resistant (7 genotypes), moderately resistant (19 genotypes), tolerant (29 genotypes), moderately susceptible (3 genotypes), and susceptible (2 genotypes). The commercial foreign cultivars of AC Sunset, AC Sterling belonged to tolerant and moderately susceptible groups, respectively. However, Saffire was classified as a tolerant genotype. The local landrace of Kooseh which is widely grown in Isfahan province was classified as susceptible genotype. Phenotypic and genetic coefficients of variation (23.85 and 18.32 %, respectively) and a relatively high broad-sense heritability (59%) for necrosis and also the phenotypic and genetic coefficients of variation (25 and 21 %, respectively) and a high broad-sense heritability (73%) for death plants indicated that there was sufficient genetic variation for resistance and selection can be effective for producing resistant genotypes to Fusarium root rot disease.
H Askarian, B Sharifnabi, M Olia, E Mahdikhani, A Akhavan,
Volume 13, Issue 47 (4-2009)
Abstract
Root knot nematodes (Meloidogyne spp.) cause yield loss in all countries, of which, M. javanica, is the most widespread species in Iran. In order to identify M. javanica, 100 infected root and soil samples of root knot nematode were collected from different regions of Kerman province. After purification of populations and identification of M. javanica based on morphological and morphometerical characters of females and second stage juveniles (J2), total DNA was extracted from eggs, J2 and female adults. Specific 670 and 1600 bp bands were amplified in all M. javanica populations using species-specific primer pairs including OPARjav / OPAFjav and Mjavf / Mjavr These specific bands could not be amplified in other species such as M. incognita and M. arenaria. It seems that, application of these species specific primers in comparison with morphological characters would be more applicable, leading to easier identification of M. javanica.
