JWSS - Journal of Water and Soil Science

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Iron chlorosis is an important disorder of plane trees (Plantanus orintalis L.) in green space of Isfahan city. Before using reclamation methods for correcting Fe chlorosis, it is necessary to find a suitable index for evaluating plant Fe status. Therefore, activity of glutathione peroxidase (GP) enzyme and concentration of carotenoids and chlorophyll a and b pigments, as physiological indices of active Fe, were compared to total Fe concentration in leaves to evaluate Fe status in plane trees. For this purpose, severity of chlorosis of plane trees at different regions of Isfahan city was recorded. Then, 73 trees with different severity of chlorosis symptoms were selected and their leaves were sampled on July 2009 and 2010. Concentration of Fe, chlorophyll a and b and carotenoids in leaves as well as activity of GP enzyme were measured. Results showed large variation in leaf Fe concentration between young and old leaves and also between leaves with different chlorosis severity symptoms. No correlation was found between leaf Fe chlorophyll concentration and severity of chlorosis symptoms. Also, concentrations of chlorophyll a in young and old leaves with very severe chlorosis symptoms were 44 and 37% smaller than that in young and old leaves with slight chlorosis symptoms, respectively. Concentration of chlorophyll b in young and old leaves with very severe chlorosis symptoms were 40 and 37% smaller than that in young and old leaves with slight chlorosis symptoms, respectively. Concentration of carotenoids varied among young and old leaves with different severity of chlorosis symptoms. Activity of GP in young leaves was also reduced by intensifying severity of chlorosis symptoms. According to the result of the present study, activity of GP enzyme and leaf concentration of carotenoids and chlorophyll a, as suitable physiological indices of active Fe, had close correlation with severity of Fe chlorosis in plane trees. Also, deficiency of active Fe in leaves is a probable reason of chlorosis symptoms in plane trees.

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In order to assess the status of the iron (Fe) chlorosis of plane trees in green space of Isfahan city, the chlorosis intensity of 73 trees in different regions was recorded as (1): low (less than 5% of leaves), (2): moderate (less than 50% of leaves), (3): severe (more than 50% of leaves), and (4): very severe (more than 95% of leaves). The whole leaf (with petiole) samples were taken from the top and bottom of each tree, separately, during 2008 and 2009. Then selected chemical properties of the leaves were analyzed.  The results showed that in more than 95 and 71% of the young leaves, Fe concentration was less than the critical deficiency level (202 mg/kg). Concentration of Fe in the older leaves of all plane trees even those with slight symptoms of chlorosis was less than the critical level (382 mg/kg). No significant relationship was found between the leaf zinc concentrations and intensity of iron chlorosis. In more than 92 (in the first year) and 87% (in the second year) of the plane trees, Zn concentration of young leaves and in all trees, Zn concentration of old leaves were less than the critical deficiency concentration (23 mg/Kg). The comparison between concentrations of Cu in the leaves with its critical deficiency level showed that more than 90% of young and old leaves accumulated toxic levels of Cu in both sampling years. In regard with the sufficient range of P, about 79% of the trees in the first year and 53% in the second year accumulated excess levels of this nutrient in their young leaves. This condition was slightly modified for the older leaves so that excessive concentration of phosphorus in mature leaves was not observed in the first year and in the second year, only about 29% of the samples had concentrations higher than critical levels.