JWSS - Journal of Water and Soil Science

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Agricultural colleges have three main objectives: training, research, and offering public services. Transfer and dissemination of agricultural knowledge and technology is the best example of universities’ services. In this regard, agricultural extension has the important role of linking agricultural colleges with agricultural industry. The linkage between extension and agricultural colleges is effective in promoting the two organizations and ultimately enhancing the development of agriculture. Therefore, the significance of this linkage has been emphasized by many program planners as well as researchers.

This descriptive research was conducted to analyze the perception of the faculty members at Agricultural College of Shiraz University and the extension agents and specialists of Fars Province. A theoretical framework was developed based on a “communication model”, and forty-seven factors effective in establishing and promoting the linkage between research and extension were recognized. The results revealed that the most important factor in research-extension linkage is “the degree of contact and knowledge of local agricultural affairs by researchers”. The paper concludes by providing recommendations that could be used to strengthen the interaction between research and extension institutions.

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A total of forty-three isolates of Ascochyta rabiei obtained from infected seeds and plants of chickpea from different areas of Kermanshah, Lorestan, Hamadan, Kordestan, and East and West Azarbaijan provinces were studied during the period 1996-1999. A rapid and simple method was developed for pathogenicity test in CDA (chickpea seed meal dextrose agar) medium. CDA medium was inoculated with different isolates and incubated at 21±1 ^oC for 9 days. The disease severity was scored as 0-100% at 24-hour intervals up to five days. Virulence forms were designated according to spectrum of disease reactions of each isolate. Thirteen isolates (IK04, IK08, IK10, IK13, IK14, IK17, IK19, IK21, IL01, IL10, IO00, IE04, IE06) were distinguished as highly virulent (HV) and 7 isolates (IK03, IK06, IK15, IE01, IE08, IL04, IL06) as weakly virulent (WV). Polygalacturonase activity of these isolates from 6 days old inoculated PZ (pectin zymogram) medium were assayed. Comparison of the results of pathogenicity test and PG activity of 43 isolates demonstrated that HV isolates show predominant enzyme activity while the WV isolates show enzyme activity.

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Improvement of feed efficiency in dairy farming economically has a great importance. In this study, the genetic parameters of net energy efficiency and its relationships with milk yield, 3.2% fat corrected milk, body weight, gross income and income over feed costs were investigated, by the 2589 monthly records collected from the 723 lactating cows in the 3 herds. The different requirements of energy were estimated, by the National Research Council (NRC) models. A general linear model was employed for determining significant factors affecting each trait. The genetic parameters were estimated by a multivariate analysis with the derivative free approach of restricted maximum likelihood algorithm. The animal models contained the fixed effects of herd-year-season, parity number and stage of lactation, the regression coefficients of each trait on the dietary levels of rumen undegradable protein and metabolizable energy, and the random effects of animal additive genetic, permanent environment and residuals. The heritability of milk yield, 3.2 percent fat corrected milk, body weight, net energy efficiency, gross income and income over feed cost, were estimates 0.31, 0.32, 0.30, 0.34, 0.24 and 0.29 respectively. The results of this study indicated that the direct selection for net energy efficiency might genetically improve the feed efficiency. It also seems that the selection for fat corrected milk may be effective for the indirect improvement of feed efficiency and economic performance of dairy cows.

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In order to determine the most important quantitative and qualitative characteristics of pomegranate fruit and its components for categorizing the genotypes, a study was carried out using 24 genotypes. In this study 28 quantitative and qualitative characteristics of pomegranate fruit and seed were evaluated. Analysis of variance showed that all of the characteristics in examined genotypes were significant, showing high variability in characteristics. Results of simple correlation analysis showed the existence of significant, positive and negative correlations among some important characteristics. Also, factor analysis showed that most of fruit juice, aril and seed characteristics constituted the main factors. Effective characteristics categorized in seven factors accounted for 89% of total variance. For each factor, eigen value more than 0.7 was considered significant. Cluster analysis was performed using these seven factors and genotypes, in the distance of 9, were divided into 5 main clusters. These groups were mainly distinct in fruit flavor and soft seededness. Furthermore, by using three main factors, genotypes positions determined in 3-plot analysis, which distinguished sweeter genotypes from sweet-sour and sour genotypes.

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Turfgrasses are the most important cover plants in the world. Quality evaluation of the turfgrasses is usually done by experienced evaluators using color texture, density and uniformity. The results obtained by different evaluators may be different, leading to researcher’s concern. Therefore, some quantitative methods have been used for increasing the aquracy and stability in results. In this study, three color evaluating methods including, spectrophotometery (chlorophyll content), chlorophyllmeter SPAD-502, and evaluator person, as well as leaf texture by evaluator person compared with leaf width were used for 75 populations of Cynodon dactylon L. and Tifdwarf cultivar. The results showed that there were significant differences between populations for the color and leaf texture. There were no significant corelations between measuring color by using specterophotometery, chlorophyllmeter-502, and evaluator. Use of SPAD-502 instrument for measuring leaf color of bermudagrass is not recommended due to small leaf width of this species. However, there was significant corelation between visual evaluation and leaf width in measuring leaf texture. Therefore, when no experienced evaluator is present, leaf width maybe used for measuring leaf texture.

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Relationship between grain filling rate , grain filling duration, yield components and other physiological traits of rice was investigated in 93 genotypes of rice at Rice Research Institute of Iran (Rasht, Iran) during the growing season of 2001-2002. The experiment was performed without any statistical design. Panicles of main stem were tagged and taken at 3- day intervals during the grain filling period. A cubic polynomial model was used to fit the grain dry weight data (mean of R²= 0.98 for all genotypes) and the grain filling rate and duration estimated for the genotypes. Traits related to sink capacity such as grain weight, grain size and grain number per panicle correlated with grain filling rate but source -capacity- related traits except chlorophyll content of flag leaf and secondary leaf did not correlate with grain filling rate. Stepwise regression showed that final grain weight, grain filling duration and flag leaf angle contributed to grain filling rate, however, path analysis showed that only final grain weight and grain filling duration had the greatest effect on grain filling rate. The results of stepwise regression irrespective of final grain weight showed that chlorophyll content of flag leaf, grain width, grain filling duration and grain length contributed to grain filling rate. Path analysis results showed that the final grain weight had the greatest direct positive effect on grain filling rate, while grain filling duration had negative effect on grain filling rate.

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Ascochyta blight caused by Ascochyta rabiei is one of the major diseases of chickpea (Cicer arientinum) in Iran. Many phytopathogenic microorganisms, incuding A .rabiei, attack their host plant by secreting pectic enzymes including polygalacturonase (PG) which causes modification of cell-wall structure, increasing accessibility of cell-wall components for degradation by other enzymes. Polygalacturonase is the major factor in the initiation of Ascochyta blight disease, therefore in this study, the enzyme was purified from a virulent isolate of A .rabiei (IK06). Fungi were cultured in PZ medium culture media were harvested and after dialysis used for purification. Purification was achieved by Carboxy Methyl Sepharose Fast Flow ion exchange column equilibrated to pH= 5.5. Zero to one molar NaCl gradient was used for elution of the proteins from the column. Determination of protein content and enzyme activity of each fraction showed that PG was eluted from the column in 0.3 to 0.4 M salt. The purity of the protein and the MW of the enzyme were determined using SDS-PAGE technique. The MW was found to be around 27 KDa. The activity of the purified protein was also evaluated using polyacrylamide gel containing pectin as substrate (zymogram gel). Optimum pH for the purified enzyme was 7.5.

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Evaluating agricultural land management practices requires a thorough knowledge of soil spatial variability and understanding their relationships. This study was conducted at a traditionally operated wheat field in Sorkhankalateh district, located about 25 km northeast of Gorgan, in Golestan province, Iran. Soil samples of the 0-30 cm depth were collected right after planting at the end of autumn 2004 , 100 × 180m plot in a nested grid pattern (n=101). A 1 m² plot of wheat was harvested at each of 101 sites previously sampled at the end of spring. Statistical results showed that frequency distribution of all data was normal. The highest and lowest CV was related to grain yield (20.40%) and pH (0.59%) respectively. Variogram analysis showed that all parameters had spatial structure and the range values showed considerable variability among the measured parameters. The ranges of spatial dependence showed a variation from 23.99m for total N up to 93.92m for K. Among the parameters, total N and ESP had stronger spatial dependence while P had the lower spatial dependence. Interpolated maps of Kriging demonstrated that crop and soil properties did not have a random pattern but had a spatial distribution. The spatial distribution of total N was similar to organic matter and also there was similarity between spatial distribution of harvest index and available P. The results demonstrated that, the spatial distribution and spatial dependence level of soil properties can be different even within similarly managed farms. Variography and Kriging can be useful tools for designing soil sampling strategies, characterizing management zones and variable application rates of inputs in the precision agriculture.

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Plant pathogenic microorganisms produce a variety of enzymes capable of degrading different polysaccharides of the plant cell walls. Pathogens use these enzymes to penetrate and colonize host cells. Polygalacturonases are thought to be the first cell wall-degrading enzymes secreted by pathogens when they grow on plant cell walls. Oligogalacturonic acids with the polymerization degrees of 10 to 13 are intermediate products of pectin degradation by the action of polygalacturonases and are known to activate plant defense responses. PG- inhibiting proteins (PGIPs) present in the cell wall of many plants increase the stability of oligogalacturonic acids in the tissues by modulating fungal PG activities. These glycoproteins of the plant cell extracellular matrix retard the advancement of fungal hyphae, reduce tissue maceration, and prevent colonization of pathogen. In this study, Phaseolus vulgaris PGIPs were extracted from hypocotyle of Derakhshan and Naz bean cultivars. PvPGIPs were purified by afinity chromatography and analyzed by SDS-PAGE. Three major bands in the range of 47-55 kDa were detected. Average yield of The affinity-purified PGIPs was 1.68 mg per 100 gram of fresh bean hypocotyle. The inhibitory effect of PGIP was assayed on the PG activities of highly virulent isolates of Fusarium oxysporum (F15) and Ascochyta rabiei (IK04). The inhibitory activity of crude PGIP from Naz and Derakhshan cultivars on polygalacturonase activity of F. oxysporum was 18 and 28 units, respectively. These inhibitory activities increased to 40 units after purification. The inhibitory effect of crude PGIPs from both these two cultivars on PG activity of A. rabiei was 9 units, while purified PGIPs inhibited this PG activity to 18 and 29 units, respectively.

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In this experiment the response of persimmon fruit, cv. Karaj, to astringency removal by ethanol and the effects of this treatment on some important fruit characteristics were investigated. Fruits were harvested at three different dates and at each date based on the previous results, and then modified treatments were applied. The best ethanol astringency removal treatment in this study was 10 ml of 38% ethanol per kg fruit for 48 hours. The period after ethanol treatments had no effect on the reduction of soluble tannin concentration, although it resulted in the reduction of flesh firmness and increased soluble pectin. Ethanol treatments and also temperature conditions reduced flesh firmness and increased ground color of fruits however, the effect of ethanol treatments was more than temperature conditions. Temperature conditions increased ethylene production, thus reducing the flesh firmness and increasing the ground color index of fruits. Ethanol treatments did not increase the ethylene production, and it seems that its effects on some characteristics are due to ethanol itself or factors other than ethylene.

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A population of offspring from a cross between ‘Golden Smoothee’ × ‘Shafi Abadi’ apple which was developed by two methods of controlled pollination (with and without covering after controlled pollination) was used to investigate the inheritance of microsatellite alleles and the necessity of covering in controlled pollination of apple. DNA was extracted from 60 seedlings (30 from each method) as well as corresponding parents and the probable source of unwanted pollen. Four microsatellite loci (CH03d12, CH03d07, CH04a12 and CH03c07) which were polymorphic among parents were selected and their florescent primers were prepared. DNA amplification was carried out using different colored florescent primers, and alleles size were determined using ABI377 automatic gene sequencer and Gene Scan Software version 2.0 according to internal standards. Results showed that all seedlings shared one allele at each locus with each parent indicating their hybrid and true to type nature. There were no differences between two methods of controlled pollination in terms of unwanted pollination and there were no off type seedling originating from unwanted pollen source. Allele distribution among the progenies showed their co-dominant mode of inheritance, and no significant difference with Mendelian co-dominant ratio (1:1:1:1) was observed using chi square (x2) test. These results showed that there was no need for covering after controlled pollination of apple at least for less sensitive cases such as cultivar breeding which takes lots of time and cost for controlled pollination of many flowers in a limited time span. Results also showed the importance and potential of microsatellite loci in detecting parent-offspring relationship prior to inheritance study of characters and alleles, bulk segregation analysis, gene and linkage map and historical reconstruction of fruit tree pedigree.

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The management and application of organic wastes in agricultural lands decreases environmental risks and increases utilization of these matters. The objective of this research was to investigate and compare the effect of polyacryl factory sewage sludge (PSS), municipal compost (MC) and cow manure (CM) on soil properties and on yield and growth of maize (single cross 704, Zea mays L.). The treatments were control (without any organic waste) and application of PSS, MC, and CM at two rates (15 and 45 t ha-1). This research was done in the research farm of Isfahan University of Technology (Lavark-Najafabad). The experimental design was a randomized, complete block with three replications. Treatments were incorporated into the soil before the maize planting (on 23 June 2008) and soil sampling was performed after 132 days (Simultaneously with maize harvesting). Application of the organic wastes resulted in significant increase soil organic matter (SOM), total nitrogen (TN) and saturated hydraulic conductivity (Ks) and significantly decreased bulk density. However, saturated hydraulic conductivity was decreased by application of PSS. Application of 15 and 45 t ha-1 of PSS reduced Ks more than 14% and 42%, respectively, compared with control however, the reduction was significant only in the plots which received 45 t ha-1 of PSS compared with control. The results of plant yield and growth showed that leaf area index (LAI), plant length, biological yield, 1000 seed weight and seed yield increase due to the application of organic wastes.

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Modernization of irrigation canals as an operation improvement tool is essential to promote the performance of canal networks and indeed requires control systems. Proportional integral derivative (PID) algorithms have more applications than the other controllers in different places of the world, but tuning these controllers for different hydraulic conditions of canals is considered as a major problem for designing control algorithms. Since the bottom slope is one of the effective factors in the water flow dynamic behavior, in this research, the distant downstream Proportional Integral Derivative feedback control with decouplers was designed with a change in longitudinal slope in a reference canal and its performance was investigated. The canal characteristics were used to tune this controller and the system identification as a new method was applied for determining canal characteristics. SOBEK hydrodynamic model modulated with MATLAB software was used to design and run the control algorithms, and slope influence on water flow behavior, tuning controller, and coefficients of controller were investigated with different values of slope. Then, controller performance for hypothetical period of operation in various scenarios was evaluated with computation performance indices. The results showed less resonance behavior of water flow and less potential of controller in steep slope

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Drought is an extended period of low precipitation which resulted in injuries to consumers of water and reducing their performance, especially in agriculture. Different indices have already been proposed for evaluating drought, based on one of the varieties of meteorological, hydrological and agricultural droughts, but no indices has been identified yet, encompassing all factors. This study has been carried out to assess existing indices for drought monitoring and proposing an integrated index including main factors of drought and is applied to the Zayandehrood river basin as study area, because of its sensitive situation in the central Iran plateau. An integrated index includes various drought factors such as meteorological, hydrological, agricultural, socio - economical and environmental factors. In designing of this integrated index, a combination of static and dynamic layers has been used. Static layers include land use, slope and soil type. Dynamic layers include precipitation, evaporation, temperature, surface water storage, groundwater levels position, and environmental needs. All these layers are analyzed in GIS software and drought zoning maps is prepared. Results showed that based on values of integrated index, water year 1371-72 was a wet year and water year 1378-79 was the most critical, in terms of drought.