JWSS - Journal of Water and Soil Science

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In a pot experiment, the effect of soybeans, mung beans and beans on the corn and sunflowers in a cadmium contaminated soil was studied in a completely randomized design with three replications. The treatments consisted of intercropping of corn and mung bean corn and beans, corn and soybean, sunflower and mung bean, sunflower and beans, sunflower and soybean monoculture of corn, and monoculture of sunflower. There were significant effects of cocropping of companion crops on shoot dry weigh, the concentration of cadmium and zinc and the chlorophyll content of corn and sunflower. In this study the shoot weight of corn grown with bean and soybean and that of sunflower grown with bean plants was decreased as compared to the related monocultures. The concentration of cadmium was increased in the corn grown with soybean or mungbean and in sunflower grown with soybean. Co- cropping with bean plants had no significant effects on the concentration of cadmium in corn or sunflower. Among companion crops, the concentration of cadmium was higher in bean plants compared to those of soybean and mungbean plants. The chlorophyll content in the leaves of corn plants grown with mungbean was increased with an increase in cadmium concentration. However, this trend was not observed in other treatments. The results of this study show that co- cropping with either soybean or mungbean may increase cadmium uptake by corn and sunflower which in turn may increase the risk of inflowing of toxin cadmium into the food chain. However, further study needs to re-evaluate the effect of these companion crops on the distribution of cadmium in the edible parts of corn and sunflower.

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Phytoremediation is one of the best methods for cleaning heavy metal contaminated soils. In this method, tolerant plants with high absorption potential are used to clean up the pollutant. The purpose of this greenhouse study was to find the most suitable plants with the highest Zn translocation factor and shoot concentration. Soil samples were collected from zinc and lead Bama mine in Isfahan province, Iran. To compare the hytoextraction power, Heliantus annus.L., Thlaspi caerulescens, Trifolium pretense L. and Amaranthus retroflexus were planted in 8 kilogeram pots. The experiment was conducted in completely randomized design with three replications. After seventy days, plants root and shoot were harvested and Zn concentration was measured. The result showed that Thlaspi caerulescens had significantly higher root and shoot Zn concentration (3125 and 4041 mg kg-1, respectively). The highest bioaccumulation factor and translocation factor was observed in Thlaspi caerulescens. Based on this research, Thlaspi caerulescens was one of the suitable Zn hyper accumulators for cleaning up the Zn pollution.

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Organic fertilizers affect soil chemical and physical properties, particularly chemical forms of zinc in soil solid phase and thereby improve soil Zn availability. The present field study was aimed to evaluate the effects of organic and chemical fertilizer (zinc sulfate) on different zinc fractions in soil solid phase of rhizosphere in two successive years in Rudasht Research Field, Isfahan. Treatments consisted of sewage sludge (5 and 10 t ha-1), cow manure (5 and 10 t ha-1), and ZnSO4 (40 Kg ha-1). The control had no added Zn. Three weeks after applying fertilizer treatments, Back Cross genotype of wheat was cultivated in each plot. Our results showed that the organic fertilizers increased Zn concentration in exchangeable fraction (EXCH-Zn), the organically bound Zn form (ORG-Zn), and Zn bound to iron and manganese oxides (FeMnOX-Zn). However, the changes in Zn fractions were dependent on the fertilizer type. Positive and significant correlation between EXCH-Zn, ORG-Zn, and FeMnOX-Zn, and the total Zn uptake by wheat indicated that these pools of Zn in solid phase are labile pools with a significant role in supplying Zn for plants.

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Management of organic and inorganic treatments may have positive or negative effects on soil quality, plant growth and human nutrition. The objectives of this study were to determine the effects of organic and inorganic zinc fertilizer application on soil quality indicators and wheat yield. This research was conducted at Agricultural Research Station Roudasht, Isfahan, Iran. Sewage sludge and cow manure (5 and 10 t/ha), ash rubber (1 t/ha), powder rubber (200 kg/ha), ZnSO4 (40 kg/ha) were applied and wheat was cultivated. Soil samples were collected at tilling and harvest stages. After taking samples and measurements of the soil parameters, we determined the critical limits for each category and class rating for the each soil parameters, and the soil quality index was calculated. The results showed sewage sludge and rubber ash were significantly effective in increasing soil bioavailable Zn compared to other treatments. Application of sewage sludge and cow manure at 10 ton/ha improved soil quality. The expanded soil quality index can help better understand the effect of fertilizers on soil. A positive and significant relationship between soil quality indicators and Zn uptake and wheat yields was also observed. Our results indicate that addition of 10 t/ha sewage sludge as fertilizer can significantly improve soil quality, supplying the necessary amount of Zn for wheat growth.

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In this work, the effect of raw and modified bentonite and zeolite with trivalent iron on the stabilization of water-soluble and adsorbed arsenic in a calcareous soil was studied. Raw and modified bentonite and zeolite were added to the soil in different weights in a completely randomized block design with three replications and kept to field capacity soil moisture content of 80% for 8 weeks. The concentrations of water-soluble and adsorbed arsenic, water-soluble and absorbed phosphorus in soil and soil pH were measured. Treatments significantly affected the mobility of arsenic and phosphorus in soil. Raw zeolite and bentonite in different levels increased arsenic mobility (about 107 to 325 % and 259 to 350% respectively). Despite the change in surface properties of zeolites modified with iron, this treatment at different levels increased arsenic mobility in soils by about 124 to 246%. Bentonite modified with iron had the greatest effect on reducing arsenic mobility in soil (about 91%). Phosphate mobility was similar to arsenic in different treatments.

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Zinc deficiency is the most widespread micronutrient disorder in the production of wheat (Triticum aestivum L.) and other cereal crops. An experiment was conducted in greenhouse, in 2013, using the sterile sand-perlite (2:1 v/v), to study the effects of two beneficial microorganisms on growth and nutritional status of wheat (Nicknejad cultivar). The study was arranged as factorial in a completely randomized design with three replications. The experimental factors consisted of Piriformospora indica (E0: Uninoculated E1: Inoculated), Pseudomonas putida (E0: Uninoculated E1: Inoculated) and Zinc (Zn0: 0 Zn1: 2µM ZnSO4 ). The results showed that inoculation by P. putida increased shoot dry weight at both levels of zinc, but this increase was observed for root dry weight only without zinc application. The iron concentration of shoot was decreased as a result of inoculation by P. putida at both levels of zinc. However, P. indica inoculation increased iron concentration in zinc application, but had no significant effect without zinc application. At both levels of zinc, the highest P, Zn, chlorophyll a and b concentrations were achieved by inoculation with P. indica. Inoculation by P. putida reduced P concentration at both levels of zinc but it reduced Zn, chlorophyll a and b concentrations only with zinc application. The results of this research showed that despite negative effect of P. putida on nutrient uptake, inoculation by P. putida and/or P. indica plays an important role in the promotion of wheat growth in zinc deficiency conditions.

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Arbuscular mycorrhizal fungi (AMF) are widespread endosymbionts in terrestrial ecosystems and their exudates have important effects on soil properties. A greenhouse experiment was conducted with six AMF treatments including four exotic species inoculums (Funneliformis mosseae ,Claroideoglomus claroideum  and Rhizophagus irregularis and a mixed isolate of three species), one mixed native AMF species treatment and a sterilized soil (control) with four salinity levels (1, 5, 10 and 15 dS m^-1). AMF increased the soil (EEG) and total (TG) extractable glomalin, and also the hot water (HWC) and diluted acid (DAC) extractable carbohydrates compared to control treatment in all salinity levels. The native AMF species had the greatest effects on EEG, TG, HWC and DAC at 10 and 15 dS m^-1. Soil EEG and TG concentrations were higher in the mixed exotic AMF treatment than in each AMF species. The greatest glomalin concentration was related to F. mosseae at 1, 5 and 15 dS m^-1 but at 10 dS m^-1 the greatest glomalin concentration was related to C. claroideum. The greatest carbohydrate concentration was related to F. mosseae at 1 and 5 dS m^-1 but at 15 dS m^-1 significant differences were observed among the three AMF species. Our results showed that there is an interaction between salinity and different AMF species, and a combination of them determines the function of AMF.

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This study was conducted to determine some mineral content concentrations in soils and plants of three elevation classes (1500, 2200 and 3000m) and two phenological stages of flowering and seedling in north facing slopes of Sabalan rangelands. Soil samples from the depth of 20cm and plant samples using 1×1m plots with 10 replications were collected. After sample preparation, the concentrations of minerals such as calcium, phosphorous, sodium, potassium, ion, copper, zinc and magnesium were determined using spectrophotometer and flame photometer. Data was analyzed by SAS9.1 software using a Completely Randomized Design with a Generalized Linear Model procedure. Results showed that elevation had a significant effect on Ca, Fe, Cu, Zn and Mn of soil and P, Na, K, Mg and Mn of plants in the study areas (P&le0.05). Growing stages had a significant effect on all elements of plants except Ca (P&le0.05). Moreover, results showed that in three elevation classes the high demand minerals' concentrations were higher at the starting seedling stage in comparison with the flowering stage. In contrast, the low demand minerals' concentrations in three elevation sites were higher in the flowering stage in comparison with seedling stage. Interaction effect of elevation and growing stage was also significant in relation to all elements except Ca (P&le0.05).

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In order to assess the status of the iron (Fe) chlorosis of plane trees in green space of Isfahan city, the chlorosis intensity of 73 trees in different regions was recorded as (1): low (less than 5% of leaves), (2): moderate (less than 50% of leaves), (3): severe (more than 50% of leaves), and (4): very severe (more than 95% of leaves). The whole leaf (with petiole) samples were taken from the top and bottom of each tree, separately, during 2008 and 2009. Then selected chemical properties of the leaves were analyzed.  The results showed that in more than 95 and 71% of the young leaves, Fe concentration was less than the critical deficiency level (202 mg/kg). Concentration of Fe in the older leaves of all plane trees even those with slight symptoms of chlorosis was less than the critical level (382 mg/kg). No significant relationship was found between the leaf zinc concentrations and intensity of iron chlorosis. In more than 92 (in the first year) and 87% (in the second year) of the plane trees, Zn concentration of young leaves and in all trees, Zn concentration of old leaves were less than the critical deficiency concentration (23 mg/Kg). The comparison between concentrations of Cu in the leaves with its critical deficiency level showed that more than 90% of young and old leaves accumulated toxic levels of Cu in both sampling years. In regard with the sufficient range of P, about 79% of the trees in the first year and 53% in the second year accumulated excess levels of this nutrient in their young leaves. This condition was slightly modified for the older leaves so that excessive concentration of phosphorus in mature leaves was not observed in the first year and in the second year, only about 29% of the samples had concentrations higher than critical levels. 

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