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H. Ghadiri, M. Majidian,
Volume 7, Issue 2 (7-2003)
Abstract

In order to investigate the effects of different nitrogen fertilizer levels and water stress during milky and dough stages on grain yield, yield components and water use efficiency of corn hybrid SC 704 (late maturing, non prolific and dent type), a field study was conducted. The factorial design of the study comprised of a randomized complete block with four replications. Four levels of nitrogen fertilizer (0, 92, 184 and 276 kg/ha nitrogen) along with three levels of irrigation (water stress imposed at milky stage, dough stage and a season-long optimum irrigation) were used as treatments. Results showed that water stress during milky and dough stages significantly decreased grain yield and thousand kernel weight. Also, effect of nitrogen fertilizer on grain yield, kernel number per ear, kernel weight per ear and thousand kernel weight was significant. Maximum grain yield was produced with 276 kg/ha nitrogen, although no significant differences were found among 92, 184 and 276 kg/ha nitrogen levels. Regarding water use efficiency during water stress, maximum efficiency was observed at milky stage but, as water stress declined with optimum irrigation, water use efficiency decreased.
A. Mojiri, A. Arzani,
Volume 7, Issue 2 (7-2003)
Abstract

In order to study the effects of different levels of nitrogen fertilizer and plant density on grain yield and its components in sunflower, an experiment was conducted using 'Record' cultivar at the Research Farm of College of Agriculture, Isfahan University of Technology in 1996. Four levels of nitrogen (0, 75, 150 and 225 kg/ha) and four plant densities (65000, 75000, 85000 and 95000 plants/ha) were used in a split plot arranged in a randomized complete block design with three replications. Developmental stages, plant height, stem diameter, head diameter, number of head per m2, grain yield, biological yield, harvest index, 1000-grain weight, number of grains per head, grain oil percentage, oil yield and grain protein content were measured. The results indicated that N fertilizer caused an extension of the growth period and means of days to physiological maturity. It also increased plant height, stem diameter and head diameter. While increasing plant density had an incremental effect on plant height, it negatively affected stem diameter and head diameter. N fertilizer up to 150 kg/ha increased the grain yield and biological yield, whereas higher levels of N fertilizer decreased both. Plant density of 85000 plants per hectare was observed as a suitable plant density, whereas the higher plant density had a negative effect on grain yield. N fertilizer via increasing the number of grains per head, and plant density via increasing the number of heads per unit area and also decreasing the number of grains per head influenced the grain yield. One-thousand grain weight was not affected by neither N fertilizer nor plant density. Considering the superiority of 150 kg/ha of N fertilizer and plant density of 85000 plants/ha for grain yield and oil yield, it appears that they could be recommended for producing desirable yield in the regions similar to the study region.
R. Amoo-Aghaie, A. Mostajeran, G. Emtiazi,
Volume 7, Issue 2 (7-2003)
Abstract

Azospirillum brasilense is one of the N2-fixing microorganisms which, in symbiosis with the roots of cereals and other graminae, enhances growth and development of plants. In this research, wheat seeds (Triticum aestivum) of three cultivars (Ghods, Roshan and Omid) were inoculated with Azospirillum strains (Sp7 and Dol). Inoculation increased growth parameters and yield of wheat cultivars, the effect being directly dependent on the strain-cultivar combination. Strain Sp7 induced the greatest grain yield, 1000-seed weight, number of grains per spike, root and shoot dry weight in Cv. Roshan, whereas strain Dol stimulated the best effect on these growth parameters in Cv. Ghods. The response of Cv. Omid was lower as compared to the other cultivars. Thus, it may be concluded that compatible strains are necessary for increasing yield and enhancing growth and development of wheat cultivars. These experiments also illustrated similar results regarding the effect of strains on N content of seeds. The observation of nitrogenase activity of Azospirillum strains in the In vitro experiments and the significant increases in N content in some inoculated cultivars support the hypothesis that biological nitrogen fixation by Azospirillum could be responsible for the observed beneficial effects on growth parameters. The comparison of nitrogenase activity of two strains showed that acetylene reduction rate for strain Dol was 1.5 times more than that for strain Sp7. Also strain Dol had more pronounced effects on growth parameters, yield and N content of grains than did strain Sp7. Strain Dol is a local strain whereas strain Sp7 is a Brazilian isolate thus, it may be concluded that the local isolates should be preferred to the foreign bacteria, as they are better suited to traditional cultivars, environment and soil conditions of the region.
T. Ghoorchi, S. Rahimi, M. Rezaeian, G. R. Ghorbani,
Volume 7, Issue 2 (7-2003)
Abstract

An experiment was carried out to estimate the potential activity of rumen anaerobic fungi in the degradation of dry matter and fiber of feeds. Samples of wheat bran, bagasse, cotton seed, alfalfa and corn silage were used as the substrates to culture rumen fungi which were isolated from a fistulated Shal sheep. Loss percentages of dry matter (DML), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent (ADL), cellulose, and hemicellulose of samples were measured after 0, 3, 6 and 9 days of incubation. Dry matter and NDF loss of substrates varied from 10.6 % to 29.4% and 11.7% to 48.7% after 9 days of fungi growth. The highest and lowest DML and NDF were related to alfalfa and bagasses, respectively. The highest values for the ADF loss (39%), hemicellulose loss (65.6%) and cellulose loss (55.6%) were measured from alfalfa. The results indicated that rumen anaerobic fungi have the ability of degrading dry matter and fiber from different types of feed.
M. Toghiani, A. Samie, A. Gheisari,
Volume 7, Issue 2 (7-2003)
Abstract

This experiment was conducted to investigate the effects of intensity of restricted early feed and increased nutrient density after restriction period on performance and compensatory growth in broilers. Two hundred and eighty-eight broiler (Lohman) chicks in a completely randomized design in a 2*3 factorial arrangement of treatments were employed. Birds were fully fed from 7 to 13d or 100% of adlibitum intake on a daily basis. The diets were diluted at 25% and 50% levels with rice hulls. After the restriction period, birds were fed with a diet, formulated to meet all their nutrient needs, according to the NRC (1994) or 10% of protein, methionine and lysine above NRC in the realimentation diet from 13 to 21d. Body weight, feed intake, daily gain and feed conversion were determined weekly and selected carcass characteristics (liver, intestine and abdominal fat pad) were measured at 49 days of age. Feed restriction significantly (P<0.01) decreased body weight gain and feed conversion during the restriction period. Birds were able to fully recover body weight depression up to 49d. The intensity of restriction had no effect on body weight, feed intake, daily gain and feed efficiency at 49d (p>0.05). Increased nutrient density had no effect on growth rate, feed intake and feed efficiency for fully-fed birds and restricted birds at 49d. The results from this experiment showed that broiler chicks may be expected to gain weight similar to chicks with fully feeding even after restricted early feed if fed with NRC diets. The effect may be due to compensatory growth.
A. Gheisari, R. Bahadoran, S. S. Tadayonfar,
Volume 7, Issue 2 (7-2003)
Abstract

In order to study the nutritive value of grades 1 and 2 wheat feed screening and macaroni wastes as energy resources, 360 21-day-old broiler chicks were divided into 36 groups of 10. In this experiment, each experimental levels of feedstuff was used at 0 (control group), 15, 30 and 45%. In addition, their apparent metabolizable energy (AMEn) was determined by Sibbald’s method. Daily feed intake of chicks fed by diets containing macaroni wastes and grade 1 wheat feed screening for 21 to 56 days of age was significantly lower than that in the control group (P<0.05). In contrast, body weight, daily weight gain and feed conversion had no significant differences during this period. However, in the total experimental period, the best feed conversion belonged to chicks fed with various levels of macaroni wastes and grade 1 wheat feed screening. Apparent metabolizable energy (AMEn) of macaroni wastes and wheat feed screening were 3700, 3270, and 2870 kcal/kg, respectively. According to the results of this experiment, it is possible to use macaroni wastes and grade 1 wheat feed screening at 45% and 30% levels in the diet of broiler chicks, respectively, without any undesirable effects on their performance.
Sh. Dokhani, J. Keramat, S. Roofigari Haghighat,
Volume 7, Issue 2 (7-2003)
Abstract

Three potato cultivars (Solanum tuberosum L.), spring Marfona, autumn Cosima and Marfona, obtained from Isfahan Province, were stored under different conditions: 4oC, darkness 12oC, fluorescent light room temperature, darkness and room temperature, daylight. Relative humidity of the above stores was 85 to 90%. Analyses were carried out to determine total glycoalkaloids, α-solanine, specific gravity and total solids, during 30 days for the spring cultivar and 90 days for the autumn cultivars in stored tubers. α -Solanine content of each cultivar was determined by HPLC, in mg per 100 g dry weight, at the beginning, the midinterval, and the end of storage periods. Also, amounts of α-solanine in autumn cultivars, which showed the highest levels after storage for sixty days, were determined before and after heat processing for frying and cooking. Data were analyzed statistically by completely randomized design with Duncan’s multiple range test. The results revealed that the amount of α-solanine of the spring cultivar considerably increased under 12oC and flourescent light as compared with the other storage conditions. The amounts of α-solanine considerably increased during the storage of autumn cultivars which were stored at 12oC under flourescent light compared with those stored at room temperature and daylight, room temperature and darkness, and at 4oC and darkness, respectively. The amount of α-solanine decreased due to spring conditions during the storage period for cultivars stored at room temperature and darkness. In spite of the decreasing effect of peeling, before frying and after cooking, frying and cooking processes had no effect on the amount of α-solanine in the samples. Therefore, according to the above results, the most appropriate storage conditions to prevent increasing effect of α-solanine in potato tubers was 4oC in darkness.
Z. Rafiei Karahroudi, B. Hatami,
Volume 7, Issue 2 (7-2003)
Abstract

Green lacewing, Chrysoperla carnea (Steph.), is one of the important natural enemies of cotton aphid, Aphis gossypii Glover. In this study, two experiments were conducted on cucumber plant in netted cages (70*70*40 cm) in a greenhouse. In the first experiment, Chryso-bags (special net bags with 360 holes per 2.5 cm2 and 8 cm in width and 12 cm in length) were used. Seven treatments of release ratios included 1:1, 1:5, 1:10, 1:15 and 1:20 (predator: prey) and two control treatments with and without empty bags were compared. The experiment was conducted once in spring and once in autumn. In the second experiment, better release ratios were compared with two methods, Chryso-bag and sawdust as a carrier of eggs. Release ratios were 1:1 and 1:5 (predator: prey). Control treatments included only empty bag and sawdust, respectively. Results of the first experiment showed that the ratios of 1:1 and 1:5 were significantly different in spring, whereas they were not in autumn. The difference was probably due to the difference in temperature. Interaction between treatments and seasons showed that the ratios of 1:1 and 1:5 were not significantly different. In the second experiment, the release ratios of 1:1 and 1:5 in sawdust with each other and with 1:1 in Chryso-bags were not significantly different, while the ratio of 1:5 in Chryso-bag compared with 1:1 in both methods were significantly different. However, leaf necrosis was observed in the sawdust treatment.
N. Loveimi, M. Almassi,
Volume 7, Issue 2 (7-2003)
Abstract

This study aims to evaluate mechanization quality and quantity for improvement purposes. Northern Ahwaz including five districts with a total area of 286,800 hectares was selected for evaluation. The current potentials for scientific mechanization and suitable production methods in the study area were identified within the framework of a field study. In addition to a holistic study of the area and the qualitative and quantitative factors, a plain with an area of 1,100 hectares was selected for a more concentrated and specific analysis of the results. Some of the factors uniquely investigated in the study area were not able to be studied in other parts of the region. The evaluation suggests that the level of mechanization in the region is 1.1 horse power/ha, which, despite its general desirability, is low for many farming technologies in the region such that most farmers use tractors for many farming activities like plowing and furrowing. In other words, the quality of mechanization in the region (worker skills, machinery management, support services, etc.) in terms of the evaluated parameters such as lower rates of tractor efficiency (40%), steep fall in reaping wheat by combine harvesters (9.4%), and a failure in farmers’ machinery training is undesirably low, far from the mechanization level in the region. The results indicate that to increase machinery efficiency, in addition to training of farmers, efforts must be addressed at providing support services. To promote the suitable technology and the level of mechanization, appropriate technologies must be identified and the required machinery and equipment should be supplied under the supervision of research and extension centers. However, regarding the low level of innovation and also low acceptance of new technologies by local farmers, it is suggested that in addition to training and extension services, typical farms must be used and mechanization service companies should be encouraged and supported in the region to supply the technologies and machinery.
S. A. M. Mirmohammady Maibody, A. Amini, J. Khajeddin,
Volume 7, Issue 2 (7-2003)
Abstract

In this study, the potential use of halophyte grasses, Aleuropus littoralis and A. lagopoides, on soil desalinization and lowering soil salinity was investigated under glasshouse conditions. The seeds of two species were collected from Rodasht area in Isfahan and grown with four salinity treatments obtained from different types of collected soils and replicated three times. Electrical conductivities of the four soil treatments were 12.4, 29.5, 43, and 69 dS/m. The results of mean comparison showed that shoot and root dry weight, sodium contents of dry weight and Na/K ratio were significantly different in both salinized species. Both species effectively reduced soil electrical conductivity by 23 to 42.5%. This was mainly due to ion absorbtion, and consequently, from Na+, Cl-, Mg2+, Ca2+ ionic reduction. In general, considerable amounts of ionic absorbtion and total soluble salt secretion through their salt glands appeared to have the most pronounced effects on decreasing soil salinity. Regarding 50% salt excretion by these species, growing these grasses could be a possible way to decrease soil salinity by grazing or harvesting salt crusted foliage from the site.
S. Dehghanian, M. Ghorbani,
Volume 7, Issue 3 (10-2003)
Abstract

In this study, efficiency of apple producers in Khorasan Province was determined by a cross sectional data of 212 apple producers. Mean technical, allocative, and economic efficiencies were estimated to be around 31, 28, and 9 percent, respectively. A high potential was also detected for increasing these efficiencies. Apple producers’ age and education had positive effects and risk aversion had a negative effect on technical efficiency. Waste reduction, optimal use of inputs, introduction of technical-extension services, and apple insurance are suggested to increase efficiencies.
S. Ayoobi, A. Jalalian, M. Karimian Eghbal,
Volume 7, Issue 3 (10-2003)
Abstract

Investigation of paleosols plays a great role in paleoecological and paleoclimatological studies. They are also important in soil survey and planning, as they exhibit characteristics different from younger soils. Paleosols are those soils which formed under conditions different from present ones, and are either buried within sedimentary sequences or those which lie on persisting surfaces. Although such soils are widespread in central Iran and Zagros Zone, they have not been studied adequately. Paleosols are identified by different parameters such as morphological, physical, chemical, mineralogical, and micromorphological characteristics. In this study, morphological, physical, and chemical properties of three paleosols from Isfahan and Chaharmahal & Bakhtiary provinces were investigated. The profiles were on different landforms including alluvial fan, dissected old plain, and old lagoonal deposits. Soil profile in Segzi site, on old lagoonal deposits, had a very dark and thick layer at a depth of 45-60 cm containing some macrofossil shells. This shows that this area was covered by brackish water during the early Holocene. In Sepahanshahr profile, presence of strong clay coating and high concentrations of CaCO3 indicates a wetter environment in the past than the present conditions with a precipitation of only 100 mm. In Emam-Gheis profile, a buried paleosol was identified with strong clay coating and free CaCO3 horizons that shows more humid conditions. Evidences obtained from the three paleosols studied indicate that effective moisture in central Iran and Zagros regions during Late Pleistocene had been higher than its present levels.
M. H. Farpour, M. K. Eghbal, H. Khademi,
Volume 7, Issue 3 (10-2003)
Abstract

Gypsiferous Aridisols are of great importance and extent in arid and semi-arid environments. There is a close relationship between soil genesis and landscape positions. This study aimed to determine the genesis and classification of gypsiferous soils and to investigate the relationship between micro-and macro-morphology of gypsum crystals and geomorphic positions in Rafsanjan area. The study area is located in Nough, 30 km north of Rafsanjan with a mean annual precipitation of 60 mm. Seven representative pedons were selected on different geomorphic positions. Physico-chemical, micromorphological, XRD, and SEM observations were performed on soil samples. Rock pediment geomorphic surfaces, that are in fact peripheries of old closed water bodies in central Iran, are the source of gypsum in the area. Large gypsum pendants and microforms of lenticular, vermiform, platy, and interlocked gypsum plates are found in rock pediment. The amount of gypsum and the size of pendants decrease moving down the slope. Lenticular and interlocked gypsum plates are found in a transition of pediment and playa. Puffy ground is observed on the saline surface of playa. Lenticular and vermiform gypsum crystals increase down the slope, but alabastrine gypsum is the most dominant form of gypsum in the playa surface. Large amounts of soft gypsum nodules are found on the rock pediment surface (western slope). In addition, spindle form of gypsum and palycrete bundles are observed in this position. Wind action played a significant role in the genesis and development of soil in rock pediment. A close relationship was found between morphology of gypsum crystals and geomorphic positions.
S. Vaseghi, M. Afyuni, H. Shariatmadari, M. Mobli,
Volume 7, Issue 3 (10-2003)
Abstract

Excessive application of sewage sludge leads to the accumulation of potentially toxic elements in soils. The objective of this greenhouse study was to examine the DTPA–extractability of Fe, Zn, Cu, Mn, Pb, Cd, Ni, and Co in relation to soil pH and to investigate the concentrations of these metals in corn (Zea mayz). The study was conducted using a factorial experiment in a completely randomized design with three replications. Sewage sludge was applied at 0, 50, 100, and 200 t/ha on four soils [Langaroud (pH= 4.8), Lahijan (pH= 5.7), Rasht (pH= 6.8), and Isfahan (pH= 7.9)]. Application of sewage sludge significantly increased DTPA-extractable Fe, Zn, Cu, Pb, Cd, and Ni in all soils. The increasing effect was in accordance with sludge level. Langaroud soil had the highest and Isfahan soil the lowest levels of DTPA-extractable metals. Application of sewage sludge increased plant growth and metals in the foliage of corn plants. The metal concentration in corn tissues also increased with decreasing soil pH. Overall, the application of sewage sludge as an organic material, particularly in acid soils, may increase availability of heavy metals, which may, in turn, result in soil pollution. Therefore, addition of sewage sludge to soils should be managed on the basis of the changes in soil heavy metal concentration.
A. A. Gheisari, O. Saraeian, M. Toghiani, D. Asadian,
Volume 7, Issue 3 (10-2003)
Abstract

This experiment was conducted to study the effects of using cotton-seed meal on the performance and carcass yield of broiler chickens at different ages. The experiment was performed in a completely randomized design with four treatments and five replicates for each treatment. The experimental treatments included the levels of 0, 3.5, 7, and 10.5% of cotton-seed meal in diets fed to chickens at 7-21 days of age. Except for the control group, these levels for grower (21-42 d of age) was increased to 7, 14, and 21% and for finisher (42-56 d of age) to 14, 21, and 21%, respectively. For the purposes of this study, 260 day-old chicks were reared in litter until 7 days of age. On day 7, chicks were divided into 20 pens with 13 chicks in each pen. NRC (1994) recommendation was used in balancing experimental diets. The chickens were weighed weekly throughout the experimental period (7-56 d). The main observations were body gain, feed intake and feed conversion ratio. Physical analysis of carcass was done at the end of the experimental period and percentages of carcass, abdominal fat, liver, pancreas and digestive tract to live body weight were calculated. The use of cotton-seed meal and the increase of the levels through different ages had no significant effects on body weight, daily gain, feed intake, feed conversion ratio, and carcass components. Increasing the level of cotton-seed meal in the diet at 7-21 days of age had significant effects on feed intake (P<0.05) and increased it. The results of this experiment indicate that we can use cotton-seed meal at levels of 7,14, and 21% of diets, respectively, in starter, grower and finisher stages without having any adverse effects on the performance and carcass yield of broiler chickens.
S. Maghsoud Lou, A. Golian, F. Eftekhar Shahroudi, M. Nassiri Mahallati, H. Kermanshahi,
Volume 7, Issue 3 (10-2003)
Abstract

An experiment with a 33 factorial arrangement in a completely randomized design with 450 day-old broilers was conducted to study the effect of energy level and time of change from starter to finisher diets on performance and economic aspects of broilers. Broiler chickens were fed at three levels of dietary energy (2800, 3000, and 3200 KcalME/Kg) from 1-42 days of age. Starter and finisher diets were changed at 16, 21, and 26 days of age and then finisher diets were fed up to 42 days of age. The effect of diet energy and changing time from starter to finisher diets on body weight and feed conversion at 26 days of age were significant (P<0.01). By increasing energy level and changing time from starter to finisher diets, at this age, body weight increased and feed conversion ratio decreased. Energy conversion decreased by decreasing the levels of energy content of diet and increasing time of change from starter to finisher diets (P<0.01). At 42 days of age, the effects of diet energy and changing time from starter to finisher diets did not show any significant effects on feed and energy conversion in contrast, body weight at this age increased significantly as a result of increasing time of change from starter to finisher diets (P<0.05). From 1-42 days of age, the interaction between diet energy and changing time from starter to finisher diets on feed and energy conversion was significant (P<0.05). The results from this study suggest that increasing diet energy and changing time from starter to finisher diets for producing broilers with less than 2 kg will improve their general performance.
A. Kheiri, A. Borhani, S. M. Okhovvat, H. Eshtiaghi,
Volume 7, Issue 3 (10-2003)
Abstract

In an etiological study on stunted, nursery grown maple seedlings, a species of root-lesion nematode (Pratylenchus vulnus) and two species of fusarium (Fusarium oxysporum and F. solani) were isolated from the infected roots. Interactions between the nematode and the two fungi in a complete randomized design with six treatments and four replications were carried out under greenhouse conditions. Nematodes were surface sterilized and reared on sterilized carrot discs. Then the maple seedlings at two-leaf stage were inoculated with a population density of 40 nematodes/100 gr soil in pots. The results indicated singnificant differences between the treatments. Seven months after inoculation, the treatments with nematode alone showed a high mortality rate of about 75% along with a severe increase in population up to about 11.2 and 1266 nematodes/gr of soil and roots, respectively. In treatments with nematode and each of the two-fungus species, the deleterious effects of fungi on nematodes were observed through decrease of nematode population. It was also shown that F. oxysporum was more effective than F. solani in nematode control.
P. Norouzi, D. Cai, M. A. Malboobi, B. Yazdi Samadi,
Volume 7, Issue 3 (10-2003)
Abstract

OF2 and VAP genes, probably involved in signal transduction of sugarbeet nematode resistance, have already been cloned in bacterial vector by AFLP molecular marker and a two-hybrid system, respectively. To examine their capability to introduce resistance in sugarbeet, the genes were transferred to plant expression vectors. For this reason, OF2 gene after isolation was inserted within T-DNA of pAM194 binary vector, downstream of CaMV35S constitutive promoter and also inserted within T-DNA of modified pBin121 binary vector, downstream of HS1pro-1 gene inducing promoter (responsible for nematode resistance). VAP gene after isolation was inserted within T-DNA of pAM194 plasmid, downstream of CaMV35S constitutive promoter. Thus, three new constructs were made in which genes of signal transduction pathway were expressed to give beet cyst nematode resistance. These plasmids were separately transferred to Agrobacterium rhizogenes, strain AR15834. In the next step, petiole explants of sugarbeet were inoculated with the bacterial cells. Transformation-derived hairy roots were analyzed by GUS staining and/or PCR and were then inoculated with nematode larvae. Primary results showed partial resistance against nematode larvae in some hairy roots. As a result, this resistance can be related to OF2 and VAP genes effect.
A. Ghazanfari-Moghaddam,
Volume 7, Issue 3 (10-2003)
Abstract

In order to increase the role of machine vision in agricultural research in Iran, especially for measuring physical attributes of seeds, a machine vision system was developed using a computer, a capture card, a video camera and a light box. All equipment was purchased from domestic markets. Computer programs were developed for hardware setup and for image processing applications. The programs perfomed tasks such as image acquisition and display, color conversion, image segmentation, object counting, and measurement of some physical attributes of the objects by analysing their images. The system was used to measure some physical attributes of pistachio nuts. The machine vision measurements were statistically compared with the measurements obtained by the conventional manual methods. The results indicated that there was generally no significant difference between the two methods. However, the time consumed by the machine vision method was far less than the time taken by manual methods. The experimental results also showed that there were many sources of error and limiting factors in using machine vision for measuring physical attributes of seeds.
O. Farhadian, M. R. Ahmadi,
Volume 7, Issue 3 (10-2003)
Abstract

Oval fertilization and survival rates at incubation stage form the most important issues of concern in rainbow trout hatcheries in Iran. Hatchery managers tend to use the best method in fertilizing the ova in order to obtain the highest survival rates. A survey of common fertilization methods may help to determine the best one. In this study, the ova from two groups of 3-5 year-old and younger than 3 years broodstocks were fertilized employing dry and semi-dry methods in normal water and dry and semi-dry methods in fertilization solution. The experiment was carried out in a completely randomized design as a 422 factorial arrangement and with two replicates. The analysis of final results indicates that the highest survival rates of eggs and larvae were obtained in semi-dry fertilization. These rates significantly increased when fertilization solution was used (P<0.05). Moreover, the survival rates of eggs and larvae of 3-5 year-old broodstocks were higher than those of younger than 3 years. On the other hand, type and methods of fertilization showed no significant differences with degree-day (sum of heat) at developmental stage but the age of broodstocks made significant differences with degree-day from fertilization to the first hatching stage (P<0.05).

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