Showing 1096 results for Ro
J. Panahandeh, S. Massiha,
Volume 8, Issue 2 (7-2004)
Abstract
The commercial potato is an autotetraploid species. Certain allotetraploid species such as S. acaule despite theire identical ploidy level are not crossable with commercial potato due to their different endosperm balance numbers. The aim of this experiment was to investigate the possibility of germplasm transferring from allotetraploid species to cultivated potato by the use of diploid species and 2n gametes. For this purpose, one clone of S. phureja was crossed with S.acaule. The resulting F1 seeds were planted the next year. For evaluation of male fertility and unreduced gametes, the pollen samples of F1 hybrids stained with acetocarmin glycerol were assessed. For chromosome counting the tips of stolons were fixed and after staining with aceto – iron- hematoxilin were squashed. For future crossing some hybrids were grafted on tomato stocks. Cytologycal observation revealed that hybrids were triplod. Male fertility of hybrids as expected for odd ploidy was low, but approximately all of them had a few large stainable pollen (putative 2n). crossing of the hybrids as male parent with S. tuberosum was not successful, but reciprocal cross was successful and 72 seeds were obtained from 380 pollination . considering EBN, it is exected that the resulting seeds originate from fertilization of unreduced gametes of triploid and n gamates of S. tuberosum to be pentaploid or near pentaploid. The applications of these hybrid in potato breeding via ploidy manipulation have been discussed.
N. Mehrdad, M. Alikhani, G. R. Ghorbani,
Volume 8, Issue 2 (7-2004)
Abstract
In a completely randomized design with a factorial arrangement (3× 5× 3), three fistulated sheep with an average body weight of 47.5 ± 2.5 kg were used to determine the effect of cutting and growing stages (no bloom , early bloom and full bloom) on chemical composition and In situ degradability of alfalfa (Medicago sativa). Nylon bags (50 µm pore size) were filled with 3.5 g of each sample and suspended in the rumen before morning feeding and incubated for seven different times (0, 2, 4, 8, 16, 24 and 48 h). The results showed that alfalfa at no bloom stage had the highest crude protein and the lowest crude fiber content. The third – cut alfalfa had the highest degradability of dry matter and organic matter. Extent of degradability depended on crude fiber and solubility. During growth stage, the protein, soluble material and degradability levels decreased while crude fiber, neutral detergent insoluble crude protein (NDICP), and acid detergent insoluble crude protein (ADICP) increased. The amount of ADICP was an estimate of unavailable crude protein in the rumen. Although the rate of organic matter degradation was maximum in full bloom, no significant difference was observed between the three stages of growth. Effective degradability (ED) of crude protein was significantly different among stages the growth (p<0.05). Effective dry matter degradability (EDDM) with a passage rate of 6%/h was significantly higher in the third cutting of alfalfa. The results of this study showed that first cutting and full bloom alfalfa had the lowest and fifth cutting and no bloom had the highest CP levels. The effect of cutting and growing stages on degradability parameters was variable and did not allow any firm conclusions to be made.
Sh. Darvishi, H. Lamea, F. Akbari Nakhjavani, F. Darvish,
Volume 8, Issue 2 (7-2004)
Abstract
The effects of growth of two strains of lactic acid bacteria on microbial flora of ground beef after packaging in air permeable packs were studied under workshop conditions. The strains isolated from dairy products were added to ground beef at a density of 104 CFU/g after identification and antibiosis test against indicator bacteria (Escherichia coli and Staphylococcus aureus). The two strains were Lactobacillus casei subsp. casei 102, and Lactococcus lactis subsp. lactis var. diacetylactis 202. Inoculated and control samples were stored for up to five days under aerobic conditions in cold storage facilities with a temperature fluctuation between of –1°C to 5°C). Enumeration of different bacterial groups (total aerobic microorganisms, lactic acid bacteria, Pseudomonas spp., coliforms, Staphylococcus aureus, fungi) and detection of Escherichia coli were carried-out during storage period. The number of lactic acid bacteria in samples inoculated with lactobacillus casei subsp. casei 102 (treatment 1) increased during storage period, whereas total number of aerobic microorganisms, Pseudomonas spp., coliforms, and yeasts reduced. In samples inoculated with Lactococcus lactis subsp. lactis var. diacetylactis 202 (treatment 2), the number of yeasts decreased considerablely. As a result, Lactobacillus casei subsp. casei 102 can be used as a suitable antagonist of spoilage and pathogenic bacteria in ground beef under aerobic Conditions in cold storage facilities with a temperature variation between –1°C to 5°C.
E. Gharizadeh, B. Hatami, H. Seyedoleslami,
Volume 8, Issue 2 (7-2004)
Abstract
The coccinellid Cryptolaemus montrouzieri Muls. is an oligophagous predator that is used to control mealy bugs in the orchards and greenhouses. Citrus soft scale, Pulvinaria aurantii, as an active pest on citrus plants in North of Iran is also attacked by this predator. This research was conducted to study biological characteristics of C. montrouzieri on citrus soft scale and mealy bug, Planococcus citri. Initially, Cryptolaemus eggs were collected from colonies of two preys and reared in laboratory (14 L: 10 D, 25±1˚C, and RH 70 ±5%). The incubation period of eggs, 1st, 2nd, 3rd and, 4th larval instars, and total larval, prepupal, and pupal stages were studied on two preys. The developmental stages on two preys were not significantly different, except for the 4th larval instar at 1% and total larval stages at 5%. The sex ratio of male to female on two preys were not significantly different at 1% and was 1:1. Except for pre-oviposition period, other parameters including female longevity, average number of eggs laid per day, average total number of eggs laid per female and percentage of hatching were significantly different at 1% on the two preys. The average feeding of total larval period on eggs of mealy bug and citrus soft scale were 5715 and 7694, respectively that, the difference was significant at 1%. The results showed that some of the biological characteristics of Cryptolaemus on two preys were significantly different. Since the predator feeding on the eggs of citrus soft scale was higher than the mealy bug, it seems that this beneficial insect could be used for biological control of the citrus soft scale.
E. Ebrahimi, J. Pourreza, S. V. Panamariov, A Kamali, A. Hosaini,
Volume 8, Issue 2 (7-2004)
Abstract
A 3×4 factorial experiment was conducted for 45 days to determine the effects of diets containing different levels of protein and fat on growth of fingerlings Beluga (Huso huso L.1758). Twelve different diets were formulated with 3 levels of protein (45,50 and 55) and 4 levels of oil including fish oil and sunflower oil (0.5 ,4 , 8 and 12). 1260 Beluga fingerlings with an initial body weight (IBW) of 2.2 gram, after adaptation to the experimental conditions, were selected and stocked randomly into 36 fiberglass tanks (100 water volume) at 35 fish per tank, and fed on experimental diets for 45 days. Two ways analysis of variance showed that the percentage of body weight increase (%BWI), specific growth rate (SGR),protein efficiency ratio (PER), food concumpsion ratio, and productive protein value (PPV) content were significantly (P<0.05) affected by different protein and fat levels. Fishes fed on diets No.3 (containing 45% protein and 14 % lipid) and No.8 (containing 50% protein and 17 % lipid) showed the highest increase in body weight percentage, the best specific growth rate, protein efficiency ratio, and lowest food concumption ratio. Besides, the highest productive protein value was observed in diet No.2 (45% protein and 10.5% lipid) and diet No.3 (45% protein and 14 % lipid).Comparison of chemical composition of examined fish corps indicated a significant difference in their protein and lipid contents (P<0.05). However, the composition of the diet, revealed no significant changes on the dry matter and ash content of the body. Based on the findings of the present study 45-50% dietary crude protein is recommended for the optimum growth of Beluga fingerling. Moreover, the rate of needed lipid for optimum growth of fingerling range is from 14 to17 percents.
M. Farshadfar, E. Farshadfar,
Volume 8, Issue 2 (7-2004)
Abstract
Agropyron is one of the most resistant plants to biotic and abiotic stresses. It plays an important role in forage yield in rangelands. Genetic variability based on different markers is the important step in crop improvement. In order to evaluate the genetic variation of different Agropyron species based on morphological and chemical traits, this experiment was carried out in the Agriculture and Natural Resources Research Center of Kermanshah. The tiller numbers, spike length, spikelet numbers, length of flag leaf, width of flag leaf, plant height, peduncle length, ash percentage, organic matter, fiber, dry matter, fat and crude protein were registered. Statistical analysis of data was done by SPSS software. According to cluster analysis the tetraploid genotypes were classified into 5 clusters. Based on principal components analysis the length of flag leaf, spike length and plant height, and among the chemical traits of the ash percentage, organic matter, and crude protein had the highest portions of variance. The genetic parameters such as PCV, GCV, ECV, Hb and Ga for length of flag leaf were 0.274, 0.169, 0.215, 0.382, 3.05 and for spike length were 30.96, 21.64,22.139, 0.48, 5.786 and for plant height were 0.16, 0.084, 0.136,0.276, 6.054, respectively.
H. R. Fooladmand, A. R. Sepaskhah, J. Niazi,
Volume 8, Issue 3 (10-2004)
Abstract
To obtain soil-moisture characteristic curve experimentally is time-consuming and usually subject to considerable errors. So, many investigators have tried to predict soil-moisture characteristic curve by different models. One of these models predicts soil moisture characteristic curve based on soil particle size distribution and bulk density. In this model, soil particle size distribution curve is divided into a number of segments, each with a specific particle radius and cumulative particle mass greater than that of the radius. Using these data, soil-moisture characteristic curve was estimated. In this model, a scale factor, α, is used which may be considered as a constant, or obtained by logistic or linear procedures. The average values of α for clay, silty clay, sandy loam, two loam soils, and two silty clay loam soils were 1.159, 1.229, 1.494, 1.391, 1.393, 1.253 and 1.254, respectively. For most conditions, soil particle size distribution curve is not available, but only the percentages of clay, silt, and sand could be obtained using soil textural data, which is not enough to draw a precise soil particle size distribution curve. In this situation, a precise soil particle size distribution curve must be initially developed on the basis of which the soil moisture characteristic curve can be predicted. In this study, using soil textural data of seven different soils, soil moisture characteristic curve of each was estimated. In these estimations, logistic and linear methods were used to obtain the α value. Then, the results were compared with those of measured soil moisture characteristic curve. For estimation of soil particle size distribution curve, two extreme values for soil particle radius, 125 and 999 m, were used. The results indicated that using particle radius of 999 µm is more appropriate. On the other hand, it was found that for clay, silty clay, and sitly clay loam texture, it is more appropriate to employ a linear equation to determine for estimating soil-moisture characteristic curve while the logistic equation can be more appropriately used for loam and sand loam textures.
J. Abedi Koupai, A. Bakhtiarifar,
Volume 8, Issue 3 (10-2004)
Abstract
Water crisis is an important issue in arid and semi-arid regions like Iran. The situation has been getting more worse over recent years drought. Hence, there is a growing need to utilize low quality water where freshwater is scarce. In this regard, urban wastewater is a promising source but there are concerns about the environmental and health aspects of using such water. The main problem regarding the performance of trickle irrigation system utilizing wastewater effluents is clogging of the emitters. In this study, field trials at pilot scale were carried out to investigate the hydraulic properties of emitters using two types of water including treated wastewater and groundwater. The trickle irrigation system consisted of three 16 mm lateral tubes placed at 300 mm spacing in the main tube and each lateral tube had 16 emitters at 300-mm spacing. The designed discharge of the emitters was 4 L/h. The hydraulic properties of the different commercial emitters (micro flapper, long path, turbo plus and Eurokey) were assessed at different time periods.
The results indicated that the Eurokey and long path emitters had the maximum and minimum emission uniformity, respectively, when applying wastewater. using groundwater, the Eurokey and turbo plus emitters had the maximum and minimum emission uniformity, respectively, during the initial phase of the study. However, the Eurokey and long path emitters had the maximum and minimum uniformity values, respectively, at the end of the study. The turbo plus emitters are regulated and, as a result, showed no evidence of sensitivity to pressure variations hence, they can be used on uneven lands and where the trickle irrigation system has long lateral tubes.
T. Mahmoodi-Ghehsareh, B. E. Sayed-Tabatabaei, C. Ghobadi, A. Mirlohi,
Volume 8, Issue 3 (10-2004)
Abstract
The significance of haploid plants as genetic and plant breeding tools has been recognized for a long time. Haploid production techniques including anther culture, isolated microspore culture and intergeneric hybridization between wheat × Hordeum bulbosum and wheat × maize have been used to produce homozygous lines which accelerate breeding programs. In this study, wheat × maize hybridization and anther culture techniques were used for haploid production in six wheat genotypes. The results showed that 70.7% of regenerated plants through anther culture were albino plants and only 29.2 % were green, while the plants produced through wheat × maize method were all green. Ploidy variation was not observed in plants regenerated through wheat × maize hybridization. It was concluded that wheat × maize crosses would be an appropriate and practical method for haploid production in different wheat genotypes, which in comparison with the anther culture method has a higher efficiency.
A. Jafari Malekabadi, M. Afyuni, S. F. Mousavi, A. Khosravi,
Volume 8, Issue 3 (10-2004)
Abstract
In recent decades, the use of nitrogen fertilizers has increased irrespective of their effects on soil properties, agricultural products and, particularly, on environmental pollution. Nitrate easily leaches from soils into groundwater. The objective of this study was to determine temporal and spatial nitrate concentrations in groundwater in agricultural, industrial and urban regions in some parts of Isfahan Province. Water samples were collected monthly from 75 agricultural, industrial, and urban wells of Isfahan, Najaf-abad, Shahreza, Natanz and Kashan during January-May 2001. The results indicated that NO3-N concentrations in most of the regions studied were higher than the standard level (10 mg/l) and nitrate pollution must be reckoned among the most serious problems of sustainable agriculture and exploitation of groundwater resources. Average NO3-N concentration in different wells ranged from 1.03 to 50.78 mg/l (4.64 to 228.5 mg/l as nitrate). The average NO3-N concentration in groundwater of Najaf-abad, Shahreza, Isfahan and Natanz-Kashan was 17.56, 14.6, 16.04, and 8.24 mg/l and 95.5, 100, 84 and 33.3 % of total wells in these regions had nitrate concentrations above the standard level, respectively. Maximum NO3-N concentration was detected in the agricultural region south of Najaf-abad (64.6 mg/l). Nitrate pollution in most of the sampling areas was mainly linked to agricultural activities. The average NO3-N concentration in groundwater of all agricultural, industrial, and urban regions, except for urban regions of Natanz and Kashan, were above the standard level. Generally, nitrate concentration level in groundwater increased with time and was maximum in March and April.
M.r. Dehghani, M.j. Zamiri, E. Rowghani, Z. Banihashemi,
Volume 8, Issue 3 (10-2004)
Abstract
The aim of this research was to study the effect of treatment with oyster mushroom (Pleurotus sajor-caju) on digestibility of Glycyrrhiza glabra L. pulp. Pleurotus sajor-caju was inoculated on sterilized wheat grains. Two weeks after growth at 25 °C it was added to the sterilized Glycyrrhiza glabra L. pulp in nylon bags. After two weeks (at 25 °C), mycelia grew on the pulp and were used for treatment. Digestibility coefficients were measured in 12 Ghezel rams. Dry matter, crude protein (CP) and nitrogen free extract (NFE) increased, but ash content, cell wall (NDF) and lignin (ADL) decreased significantly in fungal-treated as compared with non-treated pulps. Crude fiber (CF) and ADF contents were not significantly affected by the treatment. There was a tendency for crude fat (EE ether extract) to decrease by fungal treatment (p=0.08). Mean dry matter intake and digestibility coefficient of dry matter were higher in sheep which were fed the fungal-treated pulp compared with the control group. Digestibility coefficients of CP, CF, organic matter (OM), EE, NFE, NDF, ADF and ADL were significantly greater in fungal-treated pulp than in control group. Treatment with P. sajor-caju increased the nutritive value and digestibility of Glycyrrhiza glabra L. pulp (P<0.05), but culture of this fungus was not possible on non-sterilized pulp, which hinders its field application.
J. Pourreza, M.a. Edriss, H. Khosravinia, A. Aghaee,
Volume 8, Issue 3 (10-2004)
Abstract
In a completely randomized design, the effects of six different additive compounds including limestone, hydrochloric acid, zeolite, aluminum sulfate, pad ammoniac, and fatty acids on nitrogen, pH, temperature, moisture, water soluble phosphorus, and microbial count of litter of broiler chicken were investigated. Each compound was added at a level of one kg/m2 of litter. Six–hundred-and-sixteen (616) day- old broiler chickens (both sexes) were divided into 28 groups, 22 chicks per group. Four replications were allocated to each treatment from 1 to 56 days. All groups were fed with the same diet. Litter moisture was not affected by the treatment. Experimental treatments significantly (p<0.05) influenced the litter pH on day 21. The lowest pH was obtained with aluminum sulfate and the highest pH was obtained with limestone. At the end of the experimental period, all treatments had nearly the same pH levels. Litter temperature was not affected by the treatments . Experimental treatments had a significant (p<0.05) effect on litter nitrogen and bacterial count. Correlation between litter pH and nitrogen was significant and negative (r = -0.95, p<0.05). Effect of treatments on soluble P was significant and the lowest soluble P belonged to aluminum sulfate and pad ammoniac. Correlation between soluble P and pH was positive (r = 0.48, p<0.05). The results showed that some chemical compounds can be used to reduce litter pH, nitrogen, and soluble P and their effectiveness diminishes as chicks grow older.
M. R. Akbari, H. Kermanshahi, G. A. Kalidari,
Volume 8, Issue 3 (10-2004)
Abstract
This experiment was conducted to evaluate the effects of adding acetic acid to drinking water on the performance and ileal microorganisms of broiler chickens. In a completely randomized design with 5 treatments and 5 replicates, 300 day-old commercial mail broiler chicks (ROSS) were divided into 25 groups, 12 chicks per group. Each of the five levels of acetic acid added to drinking water (0.0, 0.1, 0.2, 0.3, and 0.4%) were given to five replicates for a period of 21 days. All groups were fed a practical corn-soybean based diet. During the experiment, chickens were not fed any antibiotics or coccidiostats. The diet formulation for all groups was the same. At 14 and 28 days of age, one chicken of each replicate was weighed and killed by cervical dislocation. Ileal contents were collected and used for microbial evaluation. At 49 days of age, one bird from each pen was killed to weigh gastrointestinal tract, liver, pancreas and abdominal fat. The number of total aerobes and colliforms per gram of ileal contents were enumerated on the appropriate bacteriological media. There was no significant difference in feed intake, weight gain, feed to gain ratio, live body weight, gastrointestinal tract, abdominal fat, liver and pancreas weights (p>0.05). Also, the differences among treatments for total aerobe and coliform counts were not significant (p>0.05). Under the conditions of this study, addition of acetic acid as an organic acid into drinking water at the levels used could not affect the performance and ileal microbial counts of chickens.
M. Babashahi, Gh. Ghorbani, H. R. Rahmani,
Volume 8, Issue 3 (10-2004)
Abstract
The purpose of this study was to evaluate nutritional status in Isfahan dairy herds and the relationship between milk urea nitrogen (MUN) and fertility in the first postpartum insemination. Blood and milk samples were collected from 170 and 290 Holstein cows from 9 dairy herds, respectively. Feed samples were also collected to quantify the relationship between MUN and protein and energy content of the ration. When simple linear regression was used, a close relationship was found to exist between blood and milk urea nitrogen. Regression equations indicated crude protein percentage and protein to energy ratio in the diet only explained 7 and 10 percent of milk urea nitrogen variations, respectively. The low correlation coefficient is probably due to incorrect nutritional management and variation in diet composition in different feeding days. Logistic regression was used to determine the relationship between MUN and fertility. A negative curvilinear relationship existed between MUN and rate of pregnancy at first insemination. Since cows with MUN concentrations of 16.5 to 19 mg/dl had a better chance for pregnancy than those having either below or above these values, it seems likely that pregnancy chances for those cows having this range of MUN concentration are 1.94 and 1.63 times more than cows with less than 14 mg/dl and between 14 - 16 mg/dl, respectively.
The results of this study indicated that although MUN is a useful tool for nutritional management of dairy cows, it is not suitable for monitoring reproductive performance.
A. Nasr Esfahani, A. Mousavi Bak,
Volume 8, Issue 3 (10-2004)
Abstract
The black dot disease of potato is a fungal disease caused by Colletotrichum coccodes (syn. C. atramentarium) that produces accervuli on infected underground plant parts which appear as black dots. The disease appears at the flowering stage and develops up to the season end. The survey on black dot disease of potato in two successive years indicated that the infection percentage is 39.86 in Feraydan (Isfahan), one of the most important potato production regions in Iran. Studies on usual cropping patterns with, potato including, wheat alfa-alfa, barley and fallow showed that in terms of disease infection, one year rotation with fallow reduced the disease severity almost to half compared with control and other cropping patterns. Biological seed and soil treatment, with Trichoderma harzianum in various spores density reduced the disease in various degrees significantly and increased the potato plant growth factors including the number of stems, foliage fresh and dry weights, and also the yields in cases of spore applications, depending upon the type and application dosage. Comparative susceptibility assessments of 24 commercial potato varieties to the black dot disease indicated that the variety Desiree was the least concerned in terms of infection, followed by Scort ,Casser, Casmos, Carlita and Moren , where as Marijke, Cosima and Monalisa were highly infected in comparison to other tested verities and the rests of varieties were scored within these two ranges.
M. Khalili Mahani, H. Seyedoleslami, B. Hatami,
Volume 8, Issue 3 (10-2004)
Abstract
Elm leaf beetle, Xanthogaleruca luteola (Müller), life and fertility tables were investigated under laboratory conditions (25±2°C, 70±5%R.H. and 16L: 8D), on Ulmus carpinifolia, U.c.var.umbraculifera, U. glabra var. pendula and Celtis caucasica, in spring and summer. Since the experiments were conducted under controlled conditions, feeding on hosts with different nutritional qualities was considered to be the only cause of mortality. The aim of this study was to determine the susceptible hosts to the pest. The initial population for each life table was 100 first larval instar on 20 cm foliage which continued to the end of adult longevity. For larval and adult feeding, the foliage was replaced regularly. Larval and adult mortality and eggs number per female were recorded on a daily basis. Results showed that the net reproductive rate (R0) and intrinsic rate of increase (rm) were higher on U. carpinifolia than the other hosts in both seasons. The net reproductive rate was less than one on Celtis caucasica in spring and on U. c. var. umbraculifera in summer, which represented the negative population growth on these hosts. Therefore, U. carpinifolia was the most susceptible host to Elm leaf beetle, the other susceptible hosts being U. glabra var. pendula and U. c. var. umbraculifera, in a descending order and Celtis caucasica was the least susceptible one. .
B. Sharifnabi, G. Saeidi,
Volume 8, Issue 3 (10-2004)
Abstract
Safflower (Carthamus tinctorius L.) is one of the multi-purpose oilseed crops which has a high adaptation to different conditions such as resistance to drought and it is suited to be grown in arid and semi-arid regions such as Isfahan province. Root rot disease is an important soil-borne disease of safflower in Isfahan, which can be caused by different pathogens. The objective of this study was to determine the causal agent of safflower root rot and to evaluate different genotypes for tolerance to the disease. Different species of Fusarium were isolated from sample collections. Laboratory and greenhouse inoculations indicated that F. solani was the only pathogenic species. In this experiment, 60 genotypes of safflower including breeding lines selected from various Iranian local populations and foreign cultivars were evaluated for reaction to the disease in a randomized complete block design with three replications in greenhouse. Artificial inoculation via injection of spore suspension of F. solani (106 spores/ml) was conducted on 8-week plants and then development of necrosis and death percentage were recorded. The results showed that there were significant differences among the genotypes in terms of reaction to the disease. The most resistant and susceptible genotypes were breeding lines of IUTE14310 and IUTC121 with mean necrosis of 9.67 and 28.33 mm, and death percentage of 32 and 74, respectively. Based on the means of necrosis and death percentage, the genotypes were significantly classified in 5 distinct groups including resistant (7 genotypes), moderately resistant (19 genotypes), tolerant (29 genotypes), moderately susceptible (3 genotypes), and susceptible (2 genotypes). The commercial foreign cultivars of AC Sunset, AC Sterling belonged to tolerant and moderately susceptible groups, respectively. However, Saffire was classified as a tolerant genotype. The local landrace of Kooseh which is widely grown in Isfahan province was classified as susceptible genotype. Phenotypic and genetic coefficients of variation (23.85 and 18.32 %, respectively) and a relatively high broad-sense heritability (59%) for necrosis and also the phenotypic and genetic coefficients of variation (25 and 21 %, respectively) and a high broad-sense heritability (73%) for death plants indicated that there was sufficient genetic variation for resistance and selection can be effective for producing resistant genotypes to Fusarium root rot disease.
Z. Banihashemi, A. Sartipi,
Volume 8, Issue 3 (10-2004)
Abstract
Distribution of Phytophthora species associated with stone fruits decline in Fars was investigated. Of 36 isolates of Phytophthora recovered, 23 were identified as P. cactorum (mostly from infected crown) from almond, apricot, and peach and 13 isolates of P. nicorianae from crown and basal stem of almond and apricot from different environmental conditions. The reactions of crown and root of 6- month–old local almond cultivars, i. e., Mamaee, Moheb-Ali, Talkheh e- Najafabad (Isfahan Province), Talkheh Sadeh, and Sang Talkheh Riz from Neiriz (Fars Province), peach cultivar bitter pit and apricot cultivar Hallander from Isfahan to a virulent isolate of P. cactorum were evaluated under greenhouse conditions. The inoculum of the pathogen was obtained by growing on vermiculate – hemp seed extract for 4-6 weeks and positioned around either crown or root system. Plant height, root and total weight of plants, lesion size, plant mortality, and percentage colinization of the pathogen on root and stem base were determined. The results indicated that almond cv. Mamaee was the most susceptible and almond cv. Talkheh-e-Najfabad, peach cv. bitter pit and apricot cv. Hallander were the most resistant rootstocks to P.cactorum. Comparative reactions of root and crown of various rootstocks to P. cactorum showed that although inoculation site (root or crown) in most cultivars was not significant to most characters measured, the interaction of cultivars × site of inoculation became significant for certain characters such as plant height, lesion size, root and total plant weight and mortality, indicating different reactions by root and crown to P. cactorum in some cultivars.
S. M. J. Nazemosadat, A. R. Ghasemi,
Volume 8, Issue 4 (1-2005)
Abstract
The influence of the Sea Surface Temperatures (SSTs) on the seasonal precipitation over northern and southwestern parts of Iran was investigated. The warm, cold and base phases of the SSTs were defined and the median of precipitation during each of these phases (Rw, Rc and Rb, respectively) was determined. The magnitude of Rw/Rb, Rc/Rb and Rc/Rw were used as criteria for the assessment of the effects of the alternation of SST phases on seasonal precipitation. The results indicate that in association with cold SST phase, winter rainfall is above median over western and central parts of the coastal region, central and southern parts of Fars Province and all the stations studied in Khozestan Province. On the other hand, the prevalence of warm SST phase has caused about 20% decrease in winter precipitation over the Caspian Sea coastal area and northern parts of both Fars and Khozestan provinces. In association with warm SST phase in winter, precipitation during the following spring was found to be above normal for all the stations studied in the coastal region of the Caspian Sea. The highest sensitivity levels were found in Bandar- Anzali and Astara for which spring precipitation has increased by 80% due to the dominance of warm winter phase. However, the occurrence of boreal cold SST events causes shortage of precipitation in the eastern parts of the coastal areas along the Caspian Sea. A Possible Physical mechanisem justifying the influence of the Caspian Sea SST on the Precipitation variability was introduced. According to this mechanisem, temporal and spatial variability of the Siberian High is forced by the fluctuations in these SSTs.
A. Yamchi, F. Rastgar Jazii, C. Ghobadi, A. Mousavi, A. A. Karkhanehee,
Volume 8, Issue 4 (1-2005)
Abstract
Proline as a key osmoregulating solute in plants plays an overriding role in osmotic pressure adjustment of the cell under water stress conditions. In plant, a bifunctional enzyme delta-1-pyrroline-5-carboxylate synthetase (p5cs) promotes and directs proline synthesis during drought stress conditions. The activity of this enzyme is strongly induced to increase proline concentration within the cell to prevent the impact of water deficit. In this study, the sequence coded for p5cs enzyme under the control of the cauliflower mosaic virus 35S promoter was cloned into a plasmid containing gus and nptII genes. The construct pBI121-p5cs was then transferred into Agrobacterium tumefaciens C58 (pGV3101) and used for producing transgenic tobacco(Nicotiana tabacum cv. Xanthi) plants. The amplification of a 765 bp band within p5cs gene from transferred plants and forming deep blue color in leaf tissues of the explants indicated the successful introduction of p5cs construct into nuclear genome of tobacco plants through Agrobacterium-mediated transformation. The two-month old plants growing under normal condition besides the five-day seedlings under drought stress were subjected to determination of proline concentration. Comparison of P5cs product levels between control and water tolerated plants indicated an increase of proline of about 96.91 to 1330.891 mg/g and of 204.454 to 2039.77 mg/g in plants under normal irrigation and under drought stress, conditions respectively. The significant difference between the levels of proline product in control and transgenic plants under different growing conditions demonstrated the expression of targeted gene (p5cs) in engineered tobacco plants that may pave the way to overcome the water stress problem in agronomically useful crops.
