JWSS - Journal of Water and Soil Science

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Formation of the two- and six-rowed types in barley is predominantly controlled by alleles at a single locus (vrs1) which is located in long arm of chromosome 2H. This gene is a key character on the study of barley domestication and yield. Near-isogenic lines (NILs) of barley were produced from crosses between Kanto Nakate Gold (tow-rowed) and Azumamugi (six-rowed). The selected lines were used for screening of AFLP polymorphic bands which are linked to vrs1 locus. After screening of a total of 1792 primer combination, five polymorphic bands were identified. A construction of high resolution map around the vrs1 locus was made using recombinant inbred lines. These markers can be used for a map-based cloning of the genes at the vrs1 locus.

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In order to investigate variations of spore population, root colonization and also to determine mycorrhizal symbiosis in the root and rhizosphere of Pistachio trees (Pistacia vera) in natural forests, two study stations in Kalat (Chachaeh) and Sarakhs (Shorlogh) regions were selected. Sampling from soil and root of the trees were taken from under the canopy and from a depth of 30 cm. On a monthly basis. The roots were stained and the colonization rate and the variations of spore population were measured. Some soil characteristics including pH, moisture, organic material percentage and available phosphorus were determined, The correlation coefficients between the measured factors were calculated. The results indicated that vesicular – arbuscular mycorrhiza (VAM) was the only symbiotic mycorriza of pistachio trees. Average amounts of root colonization were 13% and 11% in Chahchaheh and Shorlogh stations, respectively. Also, average numbers of spores per 1 gram of dry soil in the above stations were 12 and 10, respectively. The correlation between the variation of spore population and colonization levels was positive but the correlation between spore population and soil moisture, organic material, available phosphorus and pH was negative.

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Two hundred Arian broiler chicks were used to investigate the effect of contents of dietary glucosinolates on performance of broiler chicks during starter (0-21 days of age) and grower periods (22-42 days of age) in a completely randomized design. The dietary glucosinolates contents in the starter period were 0 (S₁), 7.5 (S₂), 15 (S₃), 22.5 (S₄), 30 (S₅) µmol/g and in the grower period they were 0 (G₁), 5.7 (G₂), 11.4 (G₃), 17.1 (G₄), 22.8 (G₅) µmol/g. Feed intake during the grower period was affected (P<0.05) by different levels of glucosinolates in the diets and the lowest feed intake was observed in the G₅ treatment. The lowest body weight gains were observed in the S₅ and G₅ treatments during the starter and grower periods, respectively (P<0.05). The feed conversion ratio (FCR) in the S₅ treatment for the starter and the G₅ treatment for the grower period were significantly different (P<0.05) from other treatments. The correlation between the body weight gain and level of dietary glucosinolates or glucosinolate consumption was high during the starter (r = -95.3% and -94.7%, respectively) and relatively high (r = -69.5% and -72.4%, respectively) during the grower period. This experiment showed that the amount of glucosinolates in the diets of broiler chicks for the periods of starter and grower feeding system should not be more than 11.36 and 8.11µmol/g diet, respectively. Also during the starter and grower feeding periods, the highest level of rapeseed meal could be used in the broiler diets are 13 and 8 percent, respectively.

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In order to determine the effects of fetal growth rate on plasma concentrations of glucose, triglyceride, total protein, and urea in ewes, this experiment was conducted at Animal Research Station of Tehran University Agricultural College. Sixteen pregnant Varamini ewes were allocated to 4 groups (n=4). During the breeding season, ewes of group 1 were mated by Varamini ram, animals in groups 2, 3 and 4 were mated by Moghani, Afshari and Shall rams, respectively. Because of the different genetic capacities of sires, fetal growth rate was expected to be different in various groups. Ewes in all groups were fed the same ration, so it was expected that differences in fetal growth rates would affect the mobilization of maternal fat and protein storage and blood levels of their metabolites. Blood samples were collected from jugular veins of ewes during the last 8 weeks of pregnancy and first week after lambing. Plasma levels of glucose, cholestrol, and triglycerid were not different among 4 groups. Total plasma protein in ewes carrying heavier fetuses was lower than in ewes with lighter fetuses (p<0.05). Conversely, plasma urea concentrations in ewes with heavier fetuses were higher than in ewes with lighter fetuses.

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In order to investigate the effect of phytase and copper supplementaion on growth and carcass composition of common carp (Cyprinus carpio L.), in a completely randomized design with 3 ×2 factorial arrangement with three replicates the experimental diets including three levels of phytase 0, 500 and 1000 (FYT) kg^-1 and two levels of copper 0 and 15 mg/kg were tested. Diets contained 0.72 percent total phosphorus. One hundred and forty four carps with a mean weight of 207±23 g were selected and allocated in eighteen cages (8 fish per cage). The experiment was carried out for 8 weeks and 2 weeks for acclimatization. Phytase supplementation caused no significant differences in weight gain (WG), feed conversion ratio (FCR), carcass index (CI), protein efficiency ratio (PER), liver, carcass and blood composition but increased apparent phosphorous digestibility (APD) significantly (P<0.04). Copper supplementation also caused no significant differences in WG, FCR, CI, PER, APD, liver copper, carcass composition, cholesterol (Ch), triglycride (TG), phosphorus of serum but decreased liver fat significantly (P<0.004) and increased high density lipoprotein (HDL) of serum (P<0.02). Interactions between phytase and copper on WG (P<0.01), FCR (P<0.04), PER (P<0.03), liver copper (P<0.02) and TG of serum (P<0.001) were significant. This experiment suggests the presence of antagonistic effect between phytase and supplemental copper. Phytase enzyme improved some parameters of growth and carcass composition of common carp without supplemental copper but addition of copper to the diets with phytase enzyme impaired the improved performance of carp.

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The egg parasitoids are the major natural enemies of sunn pest and application of selective insecticides is one of the strategies for conservation of natural enemies. The toxicity of five different insecticides used in cereal fields to the parasitized eggs was investigated under laboratory conditions. The insecticide solutions were made based on the recommended field rates. 3-, 5- and 8- day -old parasitized eggs were dipped into insecticide solutions. Among the treated developmental stages (except for the fenirothion), 3- and 8- day- old parasitized eggs showed the highest and the lowest emergence percentages, respectively. Deltamethrin had maximum effect on emergence percentage whereas phosalone had the least hazardous effects. There was a significant difference in parasitism percentage between 3- and 8- day- old parasitized eggs in each of the 4 treatments, viz trichlorphon, fenitrothion, deltamethrin and esfenvalerate. Adults emerging from 3- day- old eggs parasitised less eggs than those emerged from 8- day- old eggs in fenitrothion, and trichlorphon treatments. All tested insecticides were highly toxic to the adult parasitoid, causing 100% mortality when the adults were exposed to sprayed areas at recommended rates.

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Representatives of fluorescent pseudomonad strains were assessed for their antagonistic activity against the blackleg of potato pathogen, Pectobacterium carotovorum subsp. atrosepticum. Tested strains belonged mainly to Pseudomonas fluorescens Biovars III, IV and V and they could produce inhibition zone on PDA medium against P. c. subsp. atrosepticum ranging from 1.5-5.5cm. Six selected strains were used in two concentrations 10⁷-10⁹ and 10¹⁰-10¹² colony forming units to control potato blackleg disease caused by P. c. subsp. atrosepticum under greenhouse conditions. Results showed that all strains applied could reduce the disease ranging from 46 to 71 percent. There was no significant difference among strains and their two concentrations under greenhouse conditions. Most of the strains applied increased potato fresh weight and all of them produced antibiotic and siderophore compounds in vitro.

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A research was conducted in Fars province Agricultural Research Center in Zarghan area from 1999 to 2002 to determine the water requirement and crop coefficient of wheat, applying lysimeter. The results indicated that the water requirements of wheat were 720, 712 and 674 mm in the years of 1999-2000, 2000-2001 and 2001-2002, respectively. Using Penman-Monteith method for estimating reference crop potential evapotranspiration, the crop coefficients for wheat at a four-stage crop growth were 0.37, 0.64, 1.10 and 0.51, respectively. Due to the inaccessibility of the whole weather data, we tried to figure out a solution to determine wheat water requirement to schedule irrigation planning for future. In this respect, we made use of a ten-day class A pan mean evaporation and crop coefficient.

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This research was conducted to measure the total factor productivity (TFP) of the rural small industries in Isfahan province comprising six types of industries: food, textile, metal, non-metal mineral, chemical, and cellulose. Among the 166 rural small industries sponsored by Jihad-Agriculture Organization of Isfahan in 2001(1380), 60 plants were selected. Cross-section information collected from the 60 plants by a questionnaire in that year confirmed the body of data for this study. The distribution of the types of the 60 selected plants followed the distribution of the types in the plant population. The analytical part of this research consisted of two sections. In the first section, the TFP of the rural small industries was established by applying Cobb-Douglas production function in which the value of the plant production was the dependent variable and the investment costs, total hours of labor, costs of raw materials, and the costs of energy and water were the independent variables. Factors affecting TFP were analyzed in the second section. The estimated coefficients of the first section and the actual data of the variables, were used to calculate the TFP for each plant. Then, another regression model was worked out in which the calculated TFPs were the dependent variable. The independent variables included the mean of the number of the employees' children, the percentage of the production employees with at least primary school level of education, annual number of the off days, dummy variable for the members of the cooperative association, and a few other variables. The results of the first section showed that the TFP of food industries was higher than that of other types of industries and there was no significant difference between the TFP of textile, metal, non metal mineral, chemical and cellulose industries. The results of the analysis for determining the factors affecting the TFP, showed that the existence of a research section in the plant, the insurance of the employees and the mean of the number of employees' children had a positive effect on the TFP. But, the level of the education of the production workers, the area of the plant buildings, and the number of the days off in plant had negative effects.

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This study was conducted to determine the problems of marketing channels of damask roses and to seek appropriate solutions to enhance marketing efficiency. The results of the study revealed that traditional and industrial rose production lacked the quality demanded by the market. The efficiency index was % 92.9 in traditional and %55 in industrial production. In addition, with regard to the marketing parameters for each type of production, the share of the factors was calculated. So we can conclude that the reducing units have the most important roles in this process. According to the study, traditional units with %47.2 had a greater share compared with the industrial units (%44.5). The results have also shown that production retailer wholesalers and middlemen shares were in the lower ranks respectively. Marketing cost coefficient results showed that %70 of the retailer selling price for 1 kg of the product was related to the marketing costs. The costs for industrial units with high and low capacity were %67.7 and %65.4, respectively.

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In this research the effect of ethinyl estradiol on somatic embryogenesis of Medicago sativa was investigated. Seeds were grown in vitro on MS medium containing 2,4-D, Kin and NAA for callus induction. Plant regeneration media with 14 different combinations of auxin, cytokinin and ethinyl estradiol were provided. Three to four-week old celli and stem or hypocotyl segments of Medicago sativa were transferred to regeneration media. Results showed that ethinyl estradiol (l mg/L) induced shoot, root and somatic embryo on calli. When the stem and hypocotyl segments were cultured on medium M12, only shoots and roots were produced with no somatic embryo. Finally, it was observed that ethinyl stradiol and auxin together could induce somatic embryogenesis and plant regeneration, while these hormones had no effect separately.

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Of all types of xenobiotics, pesticides such as herbicides play a significant role in soil and water pollution due to their widely usage all over the world. This study addresses the ability of organic amendments to enhance atrazine and metamitron degradation in two herbicide contaminated soils with contrasting textures under laboratory conditions. Soil samples were collected from surface soils with textures of sandy loam and silty clay, from northeastern part of Iran. Initial concentration of herbicides was 50 mgkg^-1 soil. Contaminated soil samples were treated by manure, compost and vermicompost at the rates of %0.5 and %2 (w/w). Residual concentrations of atrazine and metamitron were determined by HPLC at the end of incubation periods of 20,40 and 60 d. Residual concentrations of atrazine were 93, 77.8 and 72.4 % of the initial concentration after 20, 40 and 60d incubation, respectively. Residual metamitron concentrations were clearly lower than atrazine. After 20,40 and 60 d., the remaining concentrations of metamitron were 5.8, 2 and 1.2 %, respectively. Organic amendments at the rates of .5 and 2 % showed similar effects on the enhancement of herbicides degradation in soils. However, no significant effect was observed between types of organic amendments. Degradation was clearly affected by soil textures. Residual concentrations of herbicides were higher in sandy loam than in silty clay soil.

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To determine yield stability and to evaluate genotype interaction with environment interaction, 18 genotype of barley (Hordeum vulgare L.) and a control group were evaluated in a randomized complete block design with 4 replications in 3 successive years (1997-2000) at 10 research stations. Simple and combined analysis of variance revealed significant genetic differences between yield genotypes for grain yield. The results of combined analysis of variance indicated that genotypic and genotype were significant through interaction with environment. Therefore, different stability parameters including, environmental variance (S²_i), environmental coefficient of variation (C.V_i), mean of variance of interaction (θ_i), interaction variance (θ_i), equivalence ( W²_i), stability variance (σ²_i), linear regression coefficient (b_i, β_i), mean of squares of deviation from regression (S² _di) and years within location MS for a genotype, averaging over all locations (MS_y/l) were determined. Based on all the stability parameters, genotype 18 was known as the most stable one and genotypes 17 and 11 ranked lower. Genotype 5 with the highest yield was known to be the most adaptable one at fertile environments and is recommended for these locations. In addition, genotype 9 with good yield and low yield variance (1.58) and regression coefficient of less than 1 is suggested for unfertile locations.

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Potato (Solanum tuberosum L.) has relatively weak root system and requires high nitrogen fertilizer which is costly and may pose environmental pollution. This study was conducted to compare root morphological characteristics and nitrogen uptake of some potato cultivars growing in Iran. A greenhouse experiment using a completely randomized design with 3 replications and 8 potato cultivars including: Arinda, Agria, Premiere, Diamant, Concord, Marfona, Marodana and Nevita was conducted at Isfahan University of Technology, Isfahan. Virus-free seed tubers were planted and grown up to flowering stage. The plants were then harvested and their root length (RL), root average diameter (RAD) and root surface area (RSA) were determined using a Delta-T Scan image analysis system and Windias software. Root length density (RLD), root length and shoot weight ratio (R/S) were also calculated. Dry mater production and nitrogen accumulation (total nitrogen uptake) (PNA) were also determined. Soil inorganic nitrogen was measured before planting and after harvest. All measured parameters, except RAD, were significantly (p<0.05) affected by cultivar. Large differences observed between cultivars for all traits indicated genetic diversity among the studied potato cultivars. The highest and the lowest values of RL, RLD, RSA, R/S, RDW and PNA were found in Marfona and Nevita respectively. Based on cluster analysis, cultivars were divided into four different groups. Under the condition of this study, Marfona was superior whereas Arinda, Nevita, Marodana and Agria were inferior. Due to the high differences in root morphological characteristics and nitrogen uptake among potato cultivars, plant breeders may produce cultivars with larger root volumes and high nitrogen uptake.

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Data from the birth weight (BW), the average daily gain from birth to weaning (ADG) and the weaning weight (WW) of 1182, 1099 and 1099 lambs were respectively collected and applied to estimate the maternal effects on heritabilities as well as on the determination of the environmental factors. The data were collected from Shahrbabak Sheep Breeding Research Station within five year, from 1993 to 1998. The effects of the year of birth, the age of the dam and its sex on all the traits were significant. The type of birth had no effect on BW but it was significant for the other two traits. Estimates of (co)variance components and genetic parameters were obtained by restricted maximum likelihood, using single and two-trait animal models. Based on the most appropriate fitted model, direct heritability of BW, ADG and WW were estimated at 0.10± 0.06, 0.21 ±0.08 and 0.22± 0.09, respectively. The maternal heritability for the three traits was also estimated at 0.27± 0.04, 0.15 ±0.05 and 0.19 ±0.05, respectively. Direct genetic and phenotypic correlations between BW and ADG BW and WW ADG and WW were estimated at 0.85 and 0.41 0.82 and 0.48 and 0.99 and 0.99, respectively. Ignoring maternal effects in animal model caused overestimation of direct heritability. Thus maternal effects are significant sources of variation for early growth traits and their ignorance in the animal model causes inaccurate genetic evaluation of the lambs.

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Vegetable crops are exposed to repeated spraying against the pests and diseases during the growing sessions. Crops such as greenhouse cucumber are usually harvested a short time after spraying, bearing a high level of pesticides with them. This fact justifies a research on this problem. This research was carried out to determine the effects of metalaxyl (a fungicide commonly used in greenhouse) residue in cucumbers grown under traditional and modern greenhouse conditions. Cucumber plants were sprayed with two different doses: 1/1 000 and 2/1000 metalaxyl in modern and traditional greenhouses. Samples were collected 1, 2, 4, 7, 10, 14, 21 and 28 days after spraying with metalaxyl. The fungicide residue was extracted, cleaned up by TLC and the final solution was analyzed by HPLC. Data were analyzed statistically by t-test (paired observations) and Duncans multiple range test. The results revealed that the amount of metalaxyl residues increased in the first four days following spraying. This amount of the residue was 10 times higher than MRL (0.5 mg/kg), while a lower level of MRL (0.5 mg/kg) was detected 21 days after the application. Results also showed that a lower dose of application left a higher residual level than the higher one. On the other hand, no significant differences (p<0.05) were found between the amounts of residues in peeled and unpeeled cucumbers. Thus, fruit peeling does not reduce the fungicide residue in cucumber. Also, there were no significant differences (p<0.05) between the results obtained from modern and traditional greenhouses. Therefore, cucumber must either be harvested before or at least 14 days after spraying with metalaxyl.

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One of the main criteria for salt tolerance is cell membrane stability under stress. Reactive oxygen species (ROS), activity of lipoxygenase (Lox) and lipid peroxidation are considered to be destructive to cell membrane under salt stress. In this study, the effects of 0, 75 and 150 mM NaCI, over a period of 14 days on the activity of lipoxygenease, the amount of hydrogen peroxide (H₂O₂) and malon dialdehyde (MDA. as a lipid peroxidation) in the leaves of Pistacia vera L. (cv. 'Qazvini' and wild 'Sarakhs' pistachio) and 'Mastic' (P. mutica F. & M.) were studied. The results indicated that by increasing salinity Lox activity increased in the leaves of all the three rootstocks. This activity reached a climax on the 7^th day and then decreased on the 14^th day. Among the three species, Mastic reached the highest amount of the Lox activity on the 14^th day with the lowest amount of Lox reduction. The amounts of H₂O₂ in the leaves of all the three rootstocks increased, on the 14^th day the highest amount of hydrogen peroxide was found in 'Mastic' and 'Sarakhs' after treating them with 150 mM NaCl. The amounts of MDA were also reached the highest level in all three rootstocks on the 7^th and 14^th days. The results also indicated the possibility of the use of lipid peroxidation index and Lox activity for selecting salt tolerant Pistacia rootstocks. More studies are needed for understanding the biochemical changes and enzyme activities in Pistacia rootstocks under salt stress.

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Pedotransfer functions are the predictive models of a certain soil property from other easily, routinely, or cheaply measured properties. The common approach for fitting the pedotransfer functions is the use of the conventional statistical regression method. Such an approach is heavily based on the crisp obervations and also the crisp relations among variables. In the modeling natural systems, like soil, we are dealing with imprecise observations and the vague relations among the variables. Therefore, we need an appropriate algorithm for modeling such a fuzzy structures. In the present study, the fuzzy regression approach was used in order to fit some chemical and physical pedotransfer functions. The optimum regression models with the fuzzy coefficients were obtained for modeling pedotransfer functions. Sensivity analysis was carried out by using the credibility level. The results indicated that the fuzzy regression might be considered, as a suitable alternative or a complement to the statistical regression, whenever a relationship between variables is imprecise and generally when dealing with the errors due to a vaguness in regression models.

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In this study, the an AMMI analysis (additive the main effects and the multiplicative interaction effects) was used to evaluate the yield stability of nine oats genotypes (4 Canadian cultivars and 5 Turkish breeding lines) and Makoi barley (as ckeck) in the 6 environments (3 dates of planting of Oct.12, Oct.31 and Nov.21 and 3 sowing densities of 300, 375 and 450 seeds/m²). The experiment was conducted at the Research Farm of Isfahan University of Technology in 2001. For each environments, a randomized complete block design with the 3 replications was used. According to the results obtained, the main effects of genotypes, environment and genotype X environment interaction effects were statistically significant (p<0.0 1) and 70% of sum of squares was explained by the first two interaction principal components (IPCI). Biplots of genotypic and environmental components of the first and second interaction principal components and the mean yields of the genotypes and environments, pattern analysis of the two significant and also stability statistics of AMMI model (EV2 & SIPC2) revealed that Boyer and Line No. 28 were the most stable genotypes. Boyer had the lowest IPC1 and Pacer and Lines No.2 and 17 were the least stable genotypes with specific adaptation to sowing density of 300 seed/m². Lines No.32 and 28 indicated the specific adaptions to first planting date (Oct.12) Makoi and Pacer had the specific adaptation to second and third planting date and the second sowing density.

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An experiment was conducted to study the effects of substitution of the different levels (0.0, 10, 20, 30 and 40%) of oat grains for the corn with and without enzyme supplementation (β -Glucanase. 0.0 and 1%) on the performance of the broiler chickens. Four hundred and eighty (480) Arian broiler chicks were employed in a randomized complete block design with 10 treatments and 3 replications (16 chickens for each replicate) from 1-56 days of age. The chicks fed with 10 percent of oats in the diets, supplemented with enzyme, had significantly higher BW than other treatments. While with 40 % oat in diet body weight were significantly decreased. The feed conversion increased with increasing of the oats levels and the enzyme supplementation resulted in lower feed conversion. With increasing oats in the diet, the percentage of carcass weight decreased, but the pancreas weight increased significantly. Conversely, the enzyme supplementation resulted an increase in carcass weight and reduction in the pancreas weight. The percentage of the tibial ash, calcium and phosphorus were unsignificaulty decreased with the increasing of the oat but enzyme lementation resulted an increase in tibial ash, calcium and phosphorus. In the areas where the corn production is limited, including 30 percent of the oats supplemented with enzyme in the diet of the broiler chicks can be beneficial.