Showing 28 results for Control
Z. Rafiei Karahroudi, B. Hatami,
Volume 7, Issue 2 (7-2003)
Abstract
Green lacewing, Chrysoperla carnea (Steph.), is one of the important natural enemies of cotton aphid, Aphis gossypii Glover. In this study, two experiments were conducted on cucumber plant in netted cages (70*70*40 cm) in a greenhouse. In the first experiment, Chryso-bags (special net bags with 360 holes per 2.5 cm2 and 8 cm in width and 12 cm in length) were used. Seven treatments of release ratios included 1:1, 1:5, 1:10, 1:15 and 1:20 (predator: prey) and two control treatments with and without empty bags were compared. The experiment was conducted once in spring and once in autumn. In the second experiment, better release ratios were compared with two methods, Chryso-bag and sawdust as a carrier of eggs. Release ratios were 1:1 and 1:5 (predator: prey). Control treatments included only empty bag and sawdust, respectively. Results of the first experiment showed that the ratios of 1:1 and 1:5 were significantly different in spring, whereas they were not in autumn. The difference was probably due to the difference in temperature. Interaction between treatments and seasons showed that the ratios of 1:1 and 1:5 were not significantly different. In the second experiment, the release ratios of 1:1 and 1:5 in sawdust with each other and with 1:1 in Chryso-bags were not significantly different, while the ratio of 1:5 in Chryso-bag compared with 1:1 in both methods were significantly different. However, leaf necrosis was observed in the sawdust treatment.
F. Padasht Dehkaei, Sh. Mansouri Jajaei H. Rouhani,
Volume 8, Issue 1 (4-2004)
Abstract
One hundred and two microorganisms were isolated from 85 paddy soil samples of different locations in Guilan Province. Antagonistic effects of the microorganisms were tested against Gibberella fujikuroi on PDA medium. Twenty one microorganism isolates were selected for seed treatment in blotter test. Seven isolates that were satisfactorily effective in reducing pathogen colonies formed on seeds and seedlings were tested in controlling disease in a greenhouse experiment with split plot design. The antagonists were Bacillus subtilis, B. circulans, Bacillus sp., Trichoderma harzianum, T. virens (2 isolates), and F.23 (unidentified). Results showed that all antagonists effectively reduced the disease in sterilized soil compared with control but overall effectiveness of B. subtilis, T. harzianum, and T. virens was higher than others and lower than the fungicide treatment (thiophant methyl thiram W.P. 80% , 2g/L). It was found that when seeds were treated with antagonists prior to inoculation with the pathogen, the effectiveness of antagonists was significantly better than when they were applied after seed infection with the pathogen.
A. Nasr Esfahani, A. Mousavi Bak,
Volume 8, Issue 3 (10-2004)
Abstract
The black dot disease of potato is a fungal disease caused by Colletotrichum coccodes (syn. C. atramentarium) that produces accervuli on infected underground plant parts which appear as black dots. The disease appears at the flowering stage and develops up to the season end. The survey on black dot disease of potato in two successive years indicated that the infection percentage is 39.86 in Feraydan (Isfahan), one of the most important potato production regions in Iran. Studies on usual cropping patterns with, potato including, wheat alfa-alfa, barley and fallow showed that in terms of disease infection, one year rotation with fallow reduced the disease severity almost to half compared with control and other cropping patterns. Biological seed and soil treatment, with Trichoderma harzianum in various spores density reduced the disease in various degrees significantly and increased the potato plant growth factors including the number of stems, foliage fresh and dry weights, and also the yields in cases of spore applications, depending upon the type and application dosage. Comparative susceptibility assessments of 24 commercial potato varieties to the black dot disease indicated that the variety Desiree was the least concerned in terms of infection, followed by Scort ,Casser, Casmos, Carlita and Moren , where as Marijke, Cosima and Monalisa were highly infected in comparison to other tested verities and the rests of varieties were scored within these two ranges.
F. Shahryari, G. Khodakaramian, A. Heydari,
Volume 8, Issue 4 (1-2005)
Abstract
Representatives of fluorescent pseudomonad strains were assessed for their antagonistic activity against the blackleg of potato pathogen, Pectobacterium carotovorum subsp. atrosepticum. Tested strains belonged mainly to Pseudomonas fluorescens Biovars III, IV and V and they could produce inhibition zone on PDA medium against P. c. subsp. atrosepticum ranging from 1.5-5.5cm. Six selected strains were used in two concentrations 107-109 and 1010-1012 colony forming units to control potato blackleg disease caused by P. c. subsp. atrosepticum under greenhouse conditions. Results showed that all strains applied could reduce the disease ranging from 46 to 71 percent. There was no significant difference among strains and their two concentrations under greenhouse conditions. Most of the strains applied increased potato fresh weight and all of them produced antibiotic and siderophore compounds in vitro.
A. Karimi Malati, B. Hatami, H. Seyedoleslami, L. Salehi,
Volume 9, Issue 4 (1-2006)
Abstract
Fecundity and sex ratio are the important factors in the quality control of parasitoid, Trichogramma. In this research, the effect of the host egg deprivation on these factors in Trichogramma brassicae Bezdenko was evaluated. The experiment was conducted with four treatments, each in five replications in a completely randomized design. Twenty mated females (24 h old) were transferred into the glass tubes (1.5×10 cm) separately and were fed by honey 20% daily. In one treatment, parasitoids were provided with the 150 host eggs (Sitotroga cerealella) in first day, but in other treatments the 150 host eggs were offered after 2, 4 and 6 days. In order to obtain fecundity, black eggs were counted after 3 or 4 days. After emergence, adult parasitoids were recognized as male and female by their antennae and sex ratio was obtained. Results indicated that as parasitoids, Trichogramma sp., were deprived from the host eggs longer, more decrease was observed in fecundity and female ratio. Therefore, parasitoid quality was decreased by the host egg deprivation.
E. Hosseini, J. Jamalian,
Volume 10, Issue 3 (10-2006)
Abstract
Consumption of foods with low glycemic index (G.I.) by diabetic patients helps maintain their blood sugar at acceptable levels. Ordinary jam has a high G.I. The purpose of the present investigation was to prepare a dietetic jam using nutritive and non-nutritive sweeteners. The investigation was carried out in four steps. First, glycyrrhizin was extracted from licorice extract powder, its ammonium form was prepared, and its purity was assay by HPLC. Secondly, based on a completely randomized design various types of jam were prepared using different combinations of sorbitol, fructose and ammonium glycyrrhizin. Thirdly, the prepared jams and their reference controls were evaluated for color, flavor, texture and overall acceptability by a selected taste-panel and were also subjected to physical analysis using Hunter Lab colorimeter and Bostwick consistometer. Two jam preparations were finally selected on the basis of their overall acceptability. Finally, glycemic indices of the selected jams were determined using NIDDM volunteers. The results showed that ammonium-glycyrrhizin enhances the overall acceptability of the selected product (P<0.05). As G.I. values for the selected jams were low (23.5 and 23.9), their consumption by diabetic patients and their production on a commercial scale are recommended.
J. Shakarami, E. Bazgir, M. Feizian,
Volume 10, Issue 3 (10-2006)
Abstract
To search for reproducible and environment-friendly antifungal agents, essential oils of five plant species including Myrtus communis, Mentha aquatica, Vitex agnus-castus, Thymus daenensis and Artemisia aucheri were studied for their inhibition effects on the mycelial growth of plant pathogenic fungi including Fusarium oxysporum, Pythium ultimum, Rhizoctonia solani and Gaeumannomyces graminis. The experiment was conducted using a completely randomized design of factorial experiment including three essential oil concentrations and three replications at 25±2 ºC on PDA. Results revealed that R. solani with 48.83 and P.ultimum with 89.62% mean of mycelial growth inhibition were the most resistant and the most susceptible fungi to the essential oils, respectively. The essential oil of M. aquatica and T. daenensis with 93.70 and 92.74% mean of mycelial growth inhibition showed the most inhibition effect and V. agnus-castus with 33.88% was the least mycelial growth inhibitor. The essential oil of M. aquatica and T. daenensis at 20 µl/petri plate (9 cm. in diameter) completely inhibited the mycelial growth of the tested fungi. Considering the high antifungal effect, and low human and environmental hazard of essential oils, it seems that the essential oils may be used for the control of plant pathogenic fungi, or at least they may be adopted as a model for the synthesis of new antifungal agents.
M. Sabohi, Gh. Soltani, M. Zibaie,
Volume 11, Issue 1 (4-2007)
Abstract
The objective of this study was to evaluate the strategies for groundwater resources management, including competition groundwater pumping, optimal control in groundwater pumping, groundwater use and tax policy, water users' participation in groundwater pumping control, and cooperation among government and water user associations in Narimany plain located in Khorasan Province. Irrigation water demand of sugar beet growers was estimated for empirical assessment of the strategies. The results indicated that among the strategies, groundwater use and tax policy may lead growers to sustainable use of groundwater resources. Furthermore, the results showed a desirable strategy is affected by cultural, social, and economic characteristics of growers and general prevalent conditions of society. Moreover, each strategy calls for its obligations, for instance, it is necessary to have an efficient taxation system for the proposed strategy. Hence, by internalizing the external cost of groundwater overdraft (that is taxing water users) government can force them to consider the adverse effects of their decisions on other producers.
E. Karimi, H. Rouhani, D. Zafari1, Gh. Khodakaramian, M. Taghinasab,
Volume 11, Issue 41 (10-2007)
Abstract
In order to study the biological control of carnation vascular wilt disease caused by Fusarium oxysporum f.sp. dianthi, 141 bacterial strains were isolated from carnation rhizosphere, and their antagonistic activity was evaluated against fungal pathogen in dual culture method. Among the tested strains, 16 strains showed antagonistic activity seven of them with more activity were selected for further investigation. Based on phenotypic features, strains E31 and E57 were identified as Bacillus cereus E76, E93, E102 and E121 as Bacillus subtilis and E130 as Pseudomonas fluorescens bv. III. All bacterial strains inhibited mycelial growth of F. o. f. sp. dianthi by production of non-volatile and volatile metabolites under laboratory condition. Microscopical analysis showed that all strains caused deformation of pathogen mycelium, and metabolites of these strains reduced conidia production rate and as well as the ability of conidia germination. In the in vivo tests, in sterilized and nonsterilized soils, the effect of bacterial strains was studied on disease severity, percentage of healthy plants and the growth rate of plants using soil inoculating and root-dipping into bacteria-methyl cellulose mixture methods. The E57 and E121 strains, in both methods, and E130 in root-dipping method showed highest effect on decreasing of disease severity and increasing of healthy plants percentage. Strains E57, E121 and E130 significantly increased total dry weight of carnation. Maximum dry weight was obtained by E57 and E130 in soil inoculating and root –dipping methods respectively.
Sh.a. Sarani, A. Sharifi Tehrani, M. Ahmadzadeh, M. Javan Nikkhah,
Volume 11, Issue 42 (1-2008)
Abstract
Two hundred fifty seven bacterial isolates were collected from colza root and rhizosphere in Golestan, Mazandaran, Guilan and Tehran provinces. Antagonistic effect of bacterial isolates on Rhizoctonia solani, the causal agent of colza damping off, was studied using dual culture method. The results showed that, 60 isolates had the ability to inhibit the growth of R. solani on PDA medium. On the basis of the biochemical, physiological and morphological tests, isolates P1, P2 and P3 were identified as Pseudomonas fluorescens. Biocontrol mechanism studies showed that isolates produced antibiotics and volatile metabolites prevented the mycelial growth of the fungus. The isolates produced some of antimicrobial metabolites including hydrogen cyanide, protease and siderophore. Isolate P3 was more effective to inhibit the growth of the fungus in-vitro. The effect of isolates on disease reduction in comparison with control was significantly different. None of the isolates were able to prevent disease occurrence completely. Isolates applied as soil treatment had a significantly higher disease control as compared to seed treatment method. Isolate P3 had considerable effect on reduction disease in the greenhouse conditions. All isolates were capable of colonizing canola roots and so increased canola growth in free-causal agent conditions.
M. R. Bakhtiari, M. Loghavi,
Volume 12, Issue 44 (7-2008)
Abstract
In the conventional methods of herbicide and fertilizer application, a substantial amount of toxic chemicals is sprayed or broadcast into the furrows, which is later washed away by irrigation water and may contaminate ground water, human and animal environment. If herbicide and fertilizer application could be limited to the most needed parts of the field rather than overall broadcasting, the mentioned problems could be alleviated and the application efficiency could also be increased by reducing the consumption rate. In an attempt to achieve this goal, a combination cultivator capable of simultaneously performing cultivation practices such as weed control (mechanical + chemical), fertilizer application and pest control was developed and evaluated. This machine was able to perform band spraying (fungicide, insecticide and herbicide) and mechanical cultivation (within-the-row weed killing, furrow reforming and hilling) simultaneously and properly. The combination cultivator was able to reduce herbicide consumption up to 66%.
V Zabihollahi, F Maighany, M Baghestany, M Mirhady,
Volume 13, Issue 47 (4-2009)
Abstract
To study the goosegrass (Eleusine indica (L.) Gaertn.) in tall fescue (Festuca arundinacea Schreb.), an experiment in 15-year old turf was conducted during 2006 in Tehran in Sheikh Fazlollah highway using randomized complete block design with 4 replications in 1*1 m2 plots. Treatments were diclofop methyl at 2.5 and 3 Lha-1, fenoxaprop-p-ethyl at 0.8 and 1 Lha-1, clodinafop propargyl at 0.6 and 0.8 Lha-1, tralkoxydim at 1 and 1.2 Lha-1, sulfosulfuron at 27 and 35 gha-1 and untreated control. All treatments were repeated 3 times during the growing period of goosegrass. The results showed that after the last spraying, diclofop methyl at 2.5 and 3 Lha-1, fenoxaprop-p-ethyl at 0.8 and 1 Lha-1, clodinafop propargyl at 0.6 and 0.8 Lha-1 decreased goosegrass's biomass up to 81, 83.64, 81.26, 78.58, 80.27 and 81.26 percent, respectively and goosegrass's density up to 82.5, 83.13, 79.38, 79.38, 78.75 and 80.63 percent, respectively, without significant differences. Treatment after 2 times of spraying controlled goosegrass more than 80 percent. Tralkoxydim and sulfosulfuron decreased goosegrass' biomas and density about 70 and 60 percent, respectively. One week after the last spraying, diclofop methyl, fenoxaprop-p-ethyl and clodinafop propargyl treatments did not decrease tall fescue's biomass without significant differences with untreated control. Turf biomass was decreased by tralkoxydim at 1 and 1.2 Lha-1 and sulfosulfuron at 27 and 35 gha-1, by 37.54, 36.79, 40.48 and 48.55 percent, respectively. Herbicide treatments made visual rating by 49.38, 48.75, 48.75 and 50.63 percent, respectively. Overall, diclofop methyl at 2.5 Lha-1, fenoxaprop-p-ethyl at 0.8 Lha-1 and clodinafop propargyl at 0.6 Lha-1 can be recommended for goosegrass control in tall fescue because of insignificant differences between both their doses, bio-environmental problems, costs, and no damage to tall fescue.
A Gharghni, A Talei, Z Zamani, M Fatahi Moghadam, H Haj Najari, S Gardiner,
Volume 13, Issue 48 (7-2009)
Abstract
A population of offspring from a cross between ‘Golden Smoothee’ × ‘Shafi Abadi’ apple which was developed by two methods of controlled pollination (with and without covering after controlled pollination) was used to investigate the inheritance of microsatellite alleles and the necessity of covering in controlled pollination of apple. DNA was extracted from 60 seedlings (30 from each method) as well as corresponding parents and the probable source of unwanted pollen. Four microsatellite loci (CH03d12, CH03d07, CH04a12 and CH03c07) which were polymorphic among parents were selected and their florescent primers were prepared. DNA amplification was carried out using different colored florescent primers, and alleles size were determined using ABI377 automatic gene sequencer and Gene Scan Software version 2.0 according to internal standards. Results showed that all seedlings shared one allele at each locus with each parent indicating their hybrid and true to type nature. There were no differences between two methods of controlled pollination in terms of unwanted pollination and there were no off type seedling originating from unwanted pollen source. Allele distribution among the progenies showed their co-dominant mode of inheritance, and no significant difference with Mendelian co-dominant ratio (1:1:1:1) was observed using chi square (x2) test. These results showed that there was no need for covering after controlled pollination of apple at least for less sensitive cases such as cultivar breeding which takes lots of time and cost for controlled pollination of many flowers in a limited time span. Results also showed the importance and potential of microsatellite loci in detecting parent-offspring relationship prior to inheritance study of characters and alleles, bulk segregation analysis, gene and linkage map and historical reconstruction of fruit tree pedigree.
E Mahdikhani Moghadam, H Rouhani, M Flahi Rastegar,
Volume 13, Issue 48 (7-2009)
Abstract
Sugar beet cyst forming nematode (Heterodera schachtii) is one of the most important pathogens of the sugar beet in Iran. For biological control of Heterodera schachtii, 10 isolates of Trichoderma related to two species T. harzianum and T. virens were examined in laboratory and green house on eggs and cysts for two years. Results obtained from the laboratory assay showed that isolates of Trichoderma parasitized 60% eggs on average. Among them two isolates T. harzianum Bi and T. virens VM1with % 76.18 and %72.55 parasitism, respectively, showed more efficiency compared with the control. In green house, experiments were carried out in autoclaved and non autoclaved soils (field soils) separately with 12 treatments and 3 replications including non infested control (using Ragbi nematicide in field soils experiment), and infested control treated with isolates of Trichoderma using Randomized Complete Design. Then analysis of variance for the bio-control potential of isolates, final population of nematode, fresh and dry root weight, fresh and dry leaves weight inoculated with isolates of Trichoderma was carried out. The results revealed a significant difference (P<0.05) between treatments and control according to Duncan,s Multiple Range Test. T. harzianum Bi and T. virens VM1 decreased population of nematodes, and increased yield in autoclaved and field soils. In autoclaved soils, the two isolates (T. harzianum Bi and T. virens VM1) decreased population of nematodes by %76.68 and %72.65, respectively compared with the control. The Ragbi nematicide, T. harzianum Bi and T. virens VM1 decreased population of nematodes by %81.65, %75.15 and %72.85, respectively compared with the control in field soils experiments.
M. J. Monem , S. P. Ahmadpanah1 ,
Volume 16, Issue 61 (10-2012)
Abstract
There are different automatic downstream control algorithms developed to increase the flexibility of the irrigation system. CARDD control logic is one of the heuristic and distance automatic downstream control systems. In this research, mathematical model of the CARDD control logic coordinated with ICSS hydrodynamic model was developed and the CARDD control logic was tested and evaluated under different situations. In order to evaluate the performance of this control algorithm, one of the canals suggested by ASCE (canal number 2) was used. The CARDD control algorithm was tested under the operational scenarios suggested by ASCE in which the control algorithm was evaluated by intense and gradual flow changes in a newly constructed (tuned) canal. Performance indicators were calculated and analysed. In the case of gradual flow changes, the maximum diversion of the water depth from the target was about 5% and was always in permitted range. In intense flow changes, the maximum diversion of the water depth from the target was about 8% and it was recovered in about 1 hour, which is a considerable time. The charts showing the variation of water depth at each turnout and the calculated performance indicators showed the satisfactory performance of CARDD control algorithm in gradual flow changes. In intense flow changes, although the maximum variation of the water depth was limited, the system response time was relatively long.
Sh. Zamani, A. Parvaresh Rizi, S. Isapour,
Volume 17, Issue 66 (2-2014)
Abstract
Modernization of irrigation canals as an operation improvement tool is essential to promote the performance of canal networks and indeed requires control systems. Proportional integral derivative (PID) algorithms have more applications than the other controllers in different places of the world, but tuning these controllers for different hydraulic conditions of canals is considered as a major problem for designing control algorithms. Since the bottom slope is one of the effective factors in the water flow dynamic behavior, in this research, the distant downstream Proportional Integral Derivative feedback control with decouplers was designed with a change in longitudinal slope in a reference canal and its performance was investigated. The canal characteristics were used to tune this controller and the system identification as a new method was applied for determining canal characteristics. SOBEK hydrodynamic model modulated with MATLAB software was used to design and run the control algorithms, and slope influence on water flow behavior, tuning controller, and coefficients of controller were investigated with different values of slope. Then, controller performance for hypothetical period of operation in various scenarios was evaluated with computation performance indices. The results showed less resonance behavior of water flow and less potential of controller in steep slope
A. Abdolahi Arjenaki, A. A. Fadaei Tehrani,
Volume 19, Issue 71 (6-2015)
Abstract
Plant parasitic nematodes, especially root knot nematodes, cause damage to most of agricultural products, and many efforts have been done to control them. In recent years, application of industrial waste and wastewater sludge as organic fertilizers in agriculture has been increased. To investigate the effects of sewage sludge on root knot nematode pathogenicity in tomato, different weights of sewage sludge (0, 4, 8, 15 and 25% of sludge in the soil) in soil were added as a completely randomized design with six replications. Analysis variance and mean comparison of growth indices of plants showed significant effects of treatments. For example, means of stem length of plants increased in soil with more amount of sewage sludge. This relationship was also observed in other indices and nutrients elements. So using sewage increased nitrogen, phosphorous, calcium and magnesium in tomato shoots inoculated with nematodes while the change of potassium was very small. Application of sewage sludge decreased the number of galls, egg-masses and eggs in egg-mass of root knot nematode.
Z. Khosravani, S. J. Khajeddin, M. Mohebbi, A. R. Soffianian, A. H. Parsamehr,
Volume 19, Issue 72 (8-2015)
Abstract
Segzi, located in the east of Isfahan, is one of the most important centers of desertification crisis in Isfahan province. Human overtaking, land deformation and the presence of huge artificial topography in flat plain has created a very unpleasant landscape in the area. In this study, satellite images Cartosat-1 were used for mapping land degradation. By using DGPS, 9 points with appropriate distributions related to road junctions were selected. These points after Interior and exterior orientation determined as control points in Cartosat-1 pair images. To improve compliance, process of points development and production of 31 tie points was done. These points was coordinated in triangulation process and introduced as check points. Desirable RMSe, 0.3 pixel is obtained. Then DEM based on 40 points was prepared with 15×15m pixel size. The DEM, in GIS software was classified to 9 elavation classes by Natural Breaks method. The file of classified raster DEM convert to vector andcut and fill appeared as polygon that by encoding them, excavation map is produced in GIS with Kappa 0.95 and 0.97 overall accuracy. The Results of this study show that Cartosat-1 satellite images have ability for study of degraded lands and anthropogenic holes. The topographic changes caused the loss of natural vegetation and desertification in this area has developed.
H. Saghi,
Volume 21, Issue 1 (6-2017)
Abstract
The water supply networks have always been of significance to researchers as a hydraulic system of transferring and distributing water. The pressure gradient is the main reason of water transfer in networks, and in case of non-standard pressure increase, the undesirable phenomenon of leakage occurs in the network. Leakage in urban water distribution networks causes water waste and enormous financial losses. Therefore, there exists the need to manage and minimize the amount of leakage. In this study, a water distribution network is modeled using the potentialities of hydraulic analysis model, the EPANET 2.0, and, by presenting a new model, the leakage location is recognized. In order to do this, we, firstly, entered all the network parameters into the software. Then, the network was analyzed supposing the non-occurrence of leakage and the amounts of nodal pressures were measured. Moreover, the nodal pressures were estimated by creating a hypothetical leakage in one of the network nodes and analyzing the network. Finally, the position of leakage was determined by defining the leakage index and comparing it in various nodes. The results show that the suggested method is efficiently capable of predicting the leakage position in the network so it can safely replace other methods, especially destructive methods used in recognizing the leakage position in the network.
A. Moghtaderi, E. Valizadegan,
Volume 25, Issue 2 (9-2021)
Abstract
In this study, the hydraulic characteristics of the flap gates are installed at the end of the trapezoidal channels were investigated by laboratory study. In the physical model, three trapezoidal channels and four gates with different weights were used (12 gates for three channels). Based on several experiments, equations for estimating of flow rate for each side slope (each channel) were developed using dimensional analysis. In this research, five forms of discharge-stage relationship were used to obtain relationships for each side slop to estimate flow rate, separately. The results show that none of the discharge-stage relationships forms as a single relationship for all flap gates in a certain channel are suitable for estimating discharge. But the first, second, and third forms of the discharge-stage relationship are very suitable for estimating the flow rate in a certain channel for a certain flap gate. In other words, for each gate with a specific weight, a separate (unique) relationship is obtained to estimate the flow rate in the form of the first to the third discharge-stage relationship in a certain channel. To estimate of flow rate by using the first form of the discharge-stage relationship, the maximum values of statistical parameters of RMSE, ME, and MARE, among all three channels, are 0.0001 m3/s, 0.00022 m3/s, and 0.22 percent, respectively. The values of all the above statistical parameters for the first, second, and third forms of the discharge-stage relationship are presented in the article.
