JWSS - Journal of Water and Soil Science

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The effects of irrigation, dry farming and drought treatments on the activities of antioxidant enzymes including superoxide dismutase, ascorbate peroxidase, catalase and peroxidase in barley leaves at different generative growth stages under field conditions were investigated. Three senescence parameters including chlorophyll, total soluble protein and rubisco large subunit protein loss, were also studied in order to compare our results to those reported by other researchers. The results showed that leaf relative water content (RWC), chlorophyll and total soluble protein and rubisco large subunit protein content declined with leaf age and the effect of water deficit. The activity of superoxide dismutase declined with the progress of the leaf age on all treatments but ascorbate peroxidase activity declined with leaf age only in irrigated (control) plants. There were no significant differences among developmental stages in catalase activity in control plants, while catalase activity declined in the water dry farming and drought stress conditions. Peroxidase activity increased with the progress of senescence for all of treatments in such conditions. Water deficit stress triggered increases in antioxidant enzymes activities. Results showed that among all studied enzymes, peroxidase has a key role in increasing resistance to oxidative stress on both the senescence stages and drought stress condition in Hordeum vulgare.

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In order to examine the effects of increasing concentrations of beet pulp (lipogenic nutrient) substituted for barley grain (glycogenic nutrient) on productive performance, body condition score loss, and blood metabolites, 18 Holstein cows were used in a randomized complete block design. The cows were blocked based on parity. The cows were 171 ± 16 d in pregnancy, 2.76 ± 1.14 parity, and 289 ± 35 d in milk at the beginning of the experiment. The cows' body condition scores were 4.12 ± 0.35 at the beginning of the trial. The cows were assigned randomly to three dietary treatments containing 1) 23.47% barley (0% beet pulp), 2) 14.87% barley (8.6% beet pulp), or 3) 6.27% barley (17.2% beet pulp). Substituting beet pulp for barley grain didn't affect FCM 3.5%, milk protein, lactose, total solid, and SNF percentage, but milk fat percentage (4.37, 4.91, and 5.18, P < 0.003) and milk energy (0.76, 0.82, and 0.84 Mcal/kg, P < 0.02) increased as beet pulp was replaced with barley, respectively. A tendency was detected for a more negative body condition score change (0.13, -0.09, and -0.12, P < 0.13) and back fat thickness (2.5, -0.4, and -1.6 mm, P < 0.13) with added beet pulp. Plasma glucose (65.83, 58, and 57.16 mg/dl, P < 0.01) and cholesterol (157.33, 122.4, and 120.8 mg/dl, P < 0.03) decreased as beet pulp was substituted for barley grain, respectively. No difference was found in the plasma content of insulin and NEFA between treatments. Considering these results, it appears that with inclusion of beet pulp in fat cows diet during late lactation may slightly reduce body condition score safely and inhibit concomitant low production with diet diluting.

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In order to map the genomic regions affecting barley forage quantity and quality, two experiments were conducted with 72 doubled haploid lines and their two parents (‘Steptoe’ and ‘Morex’), at the Research Farms of the Faculty of Crop and Animal Sciences, University College of Agriculture and Natural Resources, University of Tehran and Agriculture and Natural Resources Research Station of Sistan, in 2007. The experiments were arranged in a randomized complete block design with two replications. Each plot consisted of six rows that were 3m in length and spaced 25cm apart. QTL analysis was conducted by Composite interval mapping (CIM) method separately for each trait in each location. The main effect of genotype was high significant for all the studied traits. Transgressive segregation in both directions (positive and negative) was observed for all the studied traits. There was a negative relationship between forage qualityrelated with quantity-related traits. Thirty-three QTLs controlling different studied traits were identified. Phenotypic variance explained by these QTLs varies from 7.07 to 39.04%. Highest LOD scores were obtained for the leaf to stem ratio on chromosome 2H. QTLs of forage quality (total digestible nutrient, dry organic matter digestibility, leaf to stem ratio, seed to forage ratio and number of tiller per plant) and quantity (plant height, forage wet and dry matter) indexes were found on chromosomes 1H, 2H, 3H, 4H, 5H, 6H and 7H. Most of mapped QTLs appear to be fairly stable between locations and can become candidates for marker-assisted selection.

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Multi-environment trial data are required to obtain stability performance parameters as selection tools for effective cultivar evaluation. The interrelationship among several stability parameters and their associations with mean yield, along with the repeatability of these parameters in consecutive years was the objective of this study. Barley yield data of 18 cultivars, proprietary of Dryland Agricultural Research Institute, evaluated in 12 locations over 2005-2007 in three locations of Iran was used for the combined analysis of variance in three datasets. I: Across locations in a single evaluation year (dataset A), II: Across locations in each of two single evaluation year (dataset B), III: Across all locations in three years (dataset C). For each dataset, cultivar phenotypic variance and other statistics were appropriately partitioned in its components. The interrelationship among the parameters and their associations with mean yield based on Spearman rank correlation was studied in each of the three single evaluation years (dataset A). Rank correlation coefficients were also used as estimates of the repeatability of these stability parameters across two year combinations (dataset B). The parameters 2 σi Shukla and 2 Wi were consistently highly correlated with each other but not with mean yield in all single and pair evaluation years. The parameters bi , 2 Si and CV were consistently highly correlated with each other but not with mean yield in all single and pair evaluation years. Result showed that Shukla variance, Wricke ecovalence, SIPC1 and ASV AMMI stability statistics have high repeatability. Thus it is suggested that more stability statistics especially nonparametric methods be used for determination of repeatability methods.

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Since malting process of barley is greatly affected by the seed endosperm structure, getting information about its anatomical characteristics along with physico-chemical properties would be very important. The aim of this study was to measure length, width, thickness, kernel density, bulk density, porosity, total nitrogen, reducing sugar, diastatic activity, pH and color changes during malting process. Results showed that width, thickness, reducing sugar and diastatic activity of the samples increased over the malting time, whereas kernel density, bulk density and total nitrogen decreased (P<0/05). Scanning electron microscopic examination of barley endosperm revealed a significant relationship between grain total nitrogen and degree of endosperm modification. Because of lower nitrogen content in Sahra malt, more digestion of cell walls and protein matrix of endosperm walls were observed.

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Chlorine and sulfate toxicity in water and soils are the main factors limiting growth and yield of most plants. Tolerance and sensitive indexes related to Cl and SO4 in irrigation water and effect of nitrogen fertilizer on barley were evaluated in a completely randomized design with three replications, under greenhouse conditions. Quantity salinity tolerance and susceptibility indices such as Mean Productivity (MP), Tolerance Index (TOL), Geometric Mean Productivity (GMP), Stress Susceptibility Index (SSI), Reduction Yield Ratio (Yr) and Stress Tolerance Index (STI) on the basis of plant yield with stress (Ys) and without stress (Yp) conditions were determined. Results showed that STI had a positive and highest significant correlation with grain and straw yields, compared to other indexes. Study of Standard Beta contents in grain and straw with STI index showed that the impact of Cl had a greater effect on reduction of salinity tolerance than SO4. Also Cl/SO4 ratios of 1:3 and 1:2 for grain and 1:2 and 1:1 for plant straw brought about highest tolerance to salinity, compared to non stress conditions. The scatter plot also confirmed such findings.

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Application of compost and compost leachate as organic fertilizers can improve plant growth, nutrients uptake and increase phytoavailability of nutrients in soil. A factorial experiment (4×4×2) was conducted in a completely randomized design to evaluate the effect of compost and compost leachate on growth and chemical composition of barley and bioavailability of some nutrients in calcareous clay loam soil and sandy soil under greenhouse conditions. Treatments consisted of four levels of compost (0, 15, 30, and 60 g kg-1), four levels of compost leachate (0, 10, 20, and 40 g kg-1), and two culture mediums (clay loam soil and sandy soil) with three replicates. Results indicated that application of compost and compost leachate significantly increased barley shoot dry matter, the number of tillers, spikes and grain yield in clay loam soil and sandy soil. The maximum barley shoots dry matter and numbers of tillers in both culture mediums were obtained at the highest level of compost or compost leachate, and the maximum number of spikes and grain yield were obtained at the lowest level of compost or compost leachate. Application of compost in clay loam soil and sandy soil increased shoot and root concentrations of nitrogen (N), phosphorus (P), Iron (Fe), manganese (Mn), zinc (Zn) and copper (Cu). However, addition of compost leachate only increased shoot concentrations of Fe and P. Increasing compost levels increased post harvest concentrations of NO3- N, P, Fe, Mn, Zn, Cu and also electrical conductivity and organic matter content of clay loam soil and sandy soil. Addition of compost leachate significantly increased concentrations of Fe, Zn, Cu and also electrical conductivity and organic matter content of both culture mediums. In both culture mediums and both compost and compost leachate treatments, post harvest concentrations of micronutrients were above critical levels. Therefore, addition of these nutrients is not required for the next cultivation. Generally, compost and compost leachate are appropriate organic fertilizers for barley production however, high salinity level in compost leachate, the rate and frequency of leachate application, particularly in sand, needs intensive control and monitoring. Prior to any fertilizer recommendation, the results of this study need to be verified under field conditions, as well.

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By modifying plants at genetical, physiological and ecological levels, entophytic fungi as the most important soil microorganisms have a pronounced growth-promoting activity and also increase plant resistance to biotic and abiotic stresses. This research was undertaken to evaluate the potential of P. indica to increase barley (Hordeumvulgare L.) resistance to lead (pb). Therefore, a greenhouse experiment with two fungus treatments (non-inoculated and P. indica inoculated) and five levels of pb (0, 25, 50, 100 and 500 mg/kg) with three replications was conducted based on a factorial design. Measurement of shoot and root dry weight showed that the growth of P. indica-colonized plants at all levels of pb treatments was higher (P < 0.05) than that of the corresponding controls. Also, chlorophyll concentration of inoculated plants with P. indica was superior to non-inoculated plants. In addition, the results showed that in contrast to the plant shoot, lead concentration in the root of P. indica-colonized plants was higher than the non-inoculated controls.

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There are new technologies such as geographic information systems (GIS) that can be effective in the optimization of irrigation water. Therefore, utilizing these resources in a desirable, effective and efficient manner to ensure sustainable development is one of the most important issues in the today's world. The aim of this study was to determine and evaluate the potential water use efficiency (WUEp) index in the plain scale by using net water requirement data, area under cultivation and yields, and to determine the comparative advantage of wheat and barley plants in Tehran, Alborz, Qom, Qazvin and Zanjan provinces by employing the geographic information system (GIS). The yields of wheat and barley products by weighted average in ArcGIS software were converted to yields in the plains scale. To calculate the potential and actual water use efficiency, we used the OPTIWAT software in order to calculate the net irrigation water and the current irrigation efficiency plains; also, the yields of wheat and barley products in the plains scale were used. Zoning water use efficiency indexes was performed by using the ArcGIS software and Kriging method. Mapping results showed a comparative advantage of crops in the west and northwest of Tehran province, south and southwest of Alborz province, southwest, north- west and center of Qazvin province, center Qom province and also, northern and southern regions of the Zanjan province, in comparison to other areas. Average potential and actual water use efficiency in the whole study area of the provinces for wheat were 1.43 and 0.58 kg m^-3, respectively, while these were 1.62 and 0.65 kg m^-3 for barley, respectively.