JWSS - Journal of Water and Soil Science

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Paleosols include soils formed under climatic condition different from the present. Although such soils are widespread in central Iran region, adequate investigations of them are yet to be carried out. Micromorphology is one of the most important tools in plaeoclimatological studies. This investigation was carried out to study microscopic features of two paleosols from Isfahan province to reconstruct the paleoclimatic condition during the Quaternary. The results of this study indicate that strong clay coatings are presented in Sepahanshahr paleosol, indicating moisture regime in the past. This paleosol is polygenetic due to calcite and gypsum accumulation during drier periods compared to clay illuviation condition. Micromorphological features in Segzi paleosol indicate that this area has experienced a swampy environment during the younger Dryas. The overall results from this study indicate that climatic oscillation evidences during Quaternary have been preserved in paleosols from Isfahan region.

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Iran is a major apple producing country. A number of industrial apple products can be made from apple. In some developed countries about 25% of apples are processed into apple sauce. Apple sauce hasn’t so far been produced by Iranian food industry, but if a formulation suitable for the taste of Iranian consumers is prepared, it can commercially succeed as a consumer product. At the first step of this study, six apple sauce formulations were made from Golden Delicious apples. Then the sensory properties of these apple sauces were evaluated by partially experienced panelists. The best formulation was chosen by a ranking test. Also, this selected apple sauce formulation was evaluated by ordinary consumers in some popular restaurants. The majority of these consumers accepted the taste and flavor of this apple sauce. The chemical, physical and microbial properties of the apple sauce formulations were determined and compared. In the final step, samples of the chosen apple sauce were stored at 4^oC±1 or 25^oC±2 for 10 months and their chemical, physical and microbial properties were determined bimonthly. The results indicated that the selected apple sauce could be stored at ambient temperature for over 10 months, without undesirable quality changes.

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Endophytic fungi, Neotyphodium spp. symbiotically infect host grasses, Fescue arundinacea Schreb. and F. pratensis Huds. and Mealybug, Phenococcus solani Ferris attacks some forage plants. To evaluate the role of these fungi to control this pest, four genotypes of F. arundinacea and two genotypes of F. pratensis were used. Plant tillers were split into two sections, one section of which was freed from endophyte using a fungicide mixture. The mixture contained 2 grams of active ingredients of Propiconazol and 1 ml of Folicur per liter of water. New tillers from endophyte-infected and endophyte-free plants were transferred into the field. The number of mealybugs was measured after the first visible sign of infestation on roots. To count mealybug, one plant hill of each plot was randomly selected. Hay yield of each plot was measured by clipping the plants 5 cm above ground level. Correlation of hay yield and other growth characteristics with the number of mealybugs on roots as an index for mealybug damage on infested plants was determined. The results indicated that endophyte-free plants were highly infested with mealybug, P. solani compared with endophyte-infected plants that were completely free of P. solani, correlation coefficients showed that there was significant negative correlation between forage yield and mealybug numbers, indicating mealybug damage on infested plants. It seems that endophytic fungi are effective biocontrol factors for some root feeding pest such as mealybug, P. solani.

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Stabilizng sand dunes has been one of the main challenges in the arid regions. So far, different kinds of mulches have been used extensively for sand dune stabilization. This study was carried out to determine the optimum composition, concentration and thickness of clay mulch for sand dune stabilization. For this purpose two soil samples from clay flats of a playa with different amount of salinity from Ardestan area were used to make clay mulches. A sand dune sample was selected as bed for applying the mulch. To select the right ingredient and treatments, clay samples were mixed with sand and different amount of water, and sprayed on sand dune bed. In addition, wheat straw was added to some mixture to test its effect on stability of the mulch. Treatments with lowest crack and highest penetration of mulch in sand bed were selected for the experiment in this study. Mulch treatments contained (1): 250g sand dune + 250g clay + 25g straw (2) 250g clay + 25g straw (3) 250g sand + 250g clay (4) 250g clay (5) 125g sand + 125g clay and (6) 125g clay. All treatments were mixed with 500ml water. The experimental design was a CRD with a 6(mulch) * 2(thickness)* 2 EC factorial method with 3 replications. The results showed that clay mulch were resistant to wind erosion, but erosion took place when they had been bombarded with sandblast. The mulches with straw showed the highest resistance to erosion as compared to other treatments. With increasing the number of mulch layers, resistant to erosion also increased. The added stability of mulch was due to the increase in mulch thickness and also increases in clay and silt content. The overall result of this study shows that the mulch with two layers and higher mixture of clay and sands was the best treatment for the stabilization of sand dunes.

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Consumption of foods with low glycemic index (G.I.) by diabetic patients helps maintain their blood sugar at acceptable levels. Ordinary jam has a high G.I. The purpose of the present investigation was to prepare a dietetic jam using nutritive and non-nutritive sweeteners. The investigation was carried out in four steps. First, glycyrrhizin was extracted from licorice extract powder, its ammonium form was prepared, and its purity was assay by HPLC. Secondly, based on a completely randomized design various types of jam were prepared using different combinations of sorbitol, fructose and ammonium glycyrrhizin. Thirdly, the prepared jams and their reference controls were evaluated for color, flavor, texture and overall acceptability by a selected taste-panel and were also subjected to physical analysis using Hunter Lab colorimeter and Bostwick consistometer. Two jam preparations were finally selected on the basis of their overall acceptability. Finally, glycemic indices of the selected jams were determined using NIDDM volunteers. The results showed that ammonium-glycyrrhizin enhances the overall acceptability of the selected product (P<0.05). As G.I. values for the selected jams were low (23.5 and 23.9), their consumption by diabetic patients and their production on a commercial scale are recommended.

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In Iran, the development of cultivated areas becomes gradually impossible due to ever-increasing population growth and urban area development. Therefore, it is very important to use the existing cultivated lands more efficiently. Land suitability evaluation makes the sustainable use of the lands feasible. The objective of this study was qualitative, quantitative and economic assessment of land suitability in Talandahst area for rainfed wheat, barley and chickpea. Talandasht plain with a surface area of 4500 ha is located southwest of Kermanshah city. The climate is semi-arid with cold winter and moderate summer. The successive stages of this research included soil survey in the field, soil analysis, qualitative and quantitative and economic evaluations of land suitability. In qualitative evaluation of land, climatic, topographic and soil suitability classes were determined according to the degree of the matching. Limitation and parametric methods were used in qualitative evaluation. Quantitative and economic evaluations made based on the observed yield and gross benefit, respectively. Based on qualitative evaluation, the studied area is marginally suitable for rainfed farming of wheat, barley and chickpea. This is due to water deficiency occurring during some stages of the growing cycle. The solution for this problem is supplementary irrigation. In addition to climate limitation, there are also topographic and soil restriction for the growth of the studied crops. On the basis of observed yield, the land units are moderately to highly suitable for rainfed wheat and barley production. Among the three named crops, the most and least profitable ones are chickpea and barley, respectively, and wheat ranks between them.

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In order to evaluate endophyte effect on plant earliness, compatible host-endophyte combinations including four genotypes of tall fescue and two genotypes of meadow fescue were selected and used in this study. One tiller-part of each genotype was treated using a fungicide mixture of Propiconazole and Folicur. New tillers of endophyte-infected and endophyte-free plants were planted in the field according to a complete block design with three replications. The traits including days to head emergence, days to 50% anthesis, days to maturity initiation, number of heads (as a weekly measurement), total seed weight, net seed weight and empty seed weigh were measured. Results showed that endophytes can enhance head emergence, maturity initiation and periodical seed yield. Days to 50% anthesis, and days to maturity initiation were also reduced. Head numbers in some genotypes increased up to 2 times. Increase in net seed yield may show some physiological alterations in plant such as hormonal balance alteration which may provide better seed setting in infected plants. Based on this study, we concluded that endophytic fungi can induce earliness in infected host plants.

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In order to assess and determine amino acids profile and their availability in different sorghum grain (SG) varieties, three SG varieties including low tannin (0.09% , LTS), medium tannin (0.19%, MTS) and high tannin (0.37%, HTS) were grown at the same location. The grains were analysed for their proximate analyses, tannin and amino acids. True amino acid availability (TAAA) was obtained by Sibbald’s method using ceacectomized single comb leghorn cockerels. The results showed that methionine and cystine were the first and the second limiting amino acids, respectively in LTS and MTS. But in HTS, lysine (0.166%) was the first and methionine (0.176%) was the second limiting amino acid. Methionine content was 0.114% , 0.182% and 0.176% for LTS, MTS and HTS, respectively. Although, crude protein was lower in HTS (10%) than the other SG, but histidine, methionine and isoleucine were higher for HTS in comparison to LTS and MTS. TAAA decreased when tannin content increased and all amino acids had a lower availability in HTS than LTS and MTS (P<0.05). Methionine in HTS and LTS and glutamic acid in MTS had the highest availability. The availability of proline was most affected by tannin, and its availability was 91.55% for LTS, 84.82% for MTS and 22.82% for HTS (P<0.05).

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Improper use of natural resources, especially soil, causes its degradation and severe soil erosion. Water erosion is an important factor causing soil degradation. Land use change of pasture would result in severe soil erosion mainly due to the reduction of vegetation cover and also surface soil disturbance. The objectives of this study were to estimate the amount of sediment, runoff and nutrient loss in four different land uses including a pasture with good vegetation cover (> 20%), a pasture with poor vegetation cover (< 10%), a currently being used dryland farm and a degraded dryland farm which is not used. Soil samples were taken from the depth of 0–10 cm in a completely randomized design with four replications. A rainfall simulator was run for two hours to estimate the amount of sediment, runoff and nutrient loss. Organic matter, total N, available P and distribution of particles size in soil and sediment were measured. The results showed that a very high degradation has occurred in the area mostly due to water erosion created as a result of overgrazing in pasture, susceptibility of geological formations and more importantly, the change of land use pasture to inefficient dryland farming. Maximum and minimum runoff was observed in the abandoned dry landfarm and pasture with good vegetation cover, respectively. Maximum sediment content was observed in dryland farm. Sediment content in dryland farm, abandoned dry landfarm and pasture with poor vegetation cover were 54.5, 21 and 10.4 times more than that in the pasture with good vegetation cover, respectively. Enrichment ratio (ER) of soil particles in sediment was highest for fine silt (2-5µm), followed by clay. A minimum of ER was obtained for sand fraction. Percentages of organic matter, total N and available P in sediment were higher in the first hour as compared to the second one. This is mainly due to the fact that fine particles are removed at the beginnings of the rainfall event. Total removal of these chemical factors was highest in dryland, intermediate in pasture with poor vegetation cover and abandoned dryland and lowest in pasture with good vegetation cover. In general, cultivation and disturbance of the pasture in the area land have caused a great decrease in soil quality and made the surface very sensitive to erosion.

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Mayonnaise sauce is a nutritious food product with high caloric content, which is usually incorporated into other products as an ingredient. The product should be produced under hygienic conditions to avoid its physical and chemical deterioration along with microbial spoilage, which mostly happens due to the lack of thermal treatment. Mayonnaise is often stored for long periods at room temperature in large plastic containers before consumption. To carry out the investigation, samples of the sauce in large plastic containers and glass jars were obtained directly from a commercial producer. After preliminary quality examinations, they were allocated into 3 lots and stored at 5, 25 or 40^oC for up to 6 months. They were then subjected to physical, chemical, microbiological and organoleptic tests at monthly intervals. The results showed no significant microbial growth even when kept at higher temperatures. Significant chemical changes (autoxidation), however, occurred in both containers at temperatures close to 40^oC. Organoleptic examinations revealed significant changes, especially in color and emulsion stability, which were more evidenced for those stored at higher temperature. It was concluded that the storage of mayonnaise sauce in large plastic containers at higher temperatures should be avoided mostly due to deterioration through physical, chemical and organoleptic changes of the sauce.

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Fescues (Festuca spp) are widely distributed in the temperate regions and are used for turf, forage and soil conservation. Though seed traits are important measures of the effects of endophytic fungi on their host, little information is available in this respect for Festuca. In this study, endophyte-infected (E⁺) and endophyte-free (E^-) clones of the six fescue genotypes were used to investigate the effects of endophyte on seed production. The relationships between seed traits plus direct and indirect effects of components on seed yield were also studied. Endophyte infection resulted in 38.1% to 249% more seed yields in some genotypes, though some were not affected by this symbiotic relationship. Correlations between traits and also results of stepwise regression were influenced by the presence of the symbiotic fungi. Path analysis showed that endophytic fungi changed direct and indirect effects of components on seed yield. In endophyte containing clones, panicle fertility had the most direct effects and number of panicle per plant and seed per panicle the most indirect effects on seed yield. In E- clones, panicle fertility had the highest indirect effects and other components had direct effects on seed yield. The results suggested that breeding strategies for increasing seed yield in fescue should consider the presence or absence of endophyte in germplasm.

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To investigate the degree of forest degradation and the effect of land use change on selected soil quality attributes in loess-derived landforms, samples were taken from different land uses including forest, rangeland, degradated rangeland and farmland in Pasang watershed located in the Galikesh area of Golestan province (37°16'N, 55°30'E). The annual average temperature and mean precipitation of study area were 15°C and 730 mm respectively. Organic matter, pH, EC, CaCO₃ and nutrients (N, P, K) as chemical indicators, hydraulic conductivity, bulk density and porosity as physical indicators and soil respiration as biological indicator were measured. The results showed that the amount of organic matter decreased three percent when it was turned from forest to farmland, and increased two percent from farmland to rangeland. The amount of CaCO₃ in surface layer of deforested area was more than in the forest soils. The amount of soil N in forest and soil P and K in rangeland were higher than in other land uses. Bulk density and porosity in forest and MWD in rangeland were higher than in other land uses because of the decrease in organic matter due to farming activities. Soil respiration in forest was highest as compared to in other land uses. Difference of enzymes activities (L-asparaginase and Dehydrogenase) compared to microbial respiration indicates that enzymes activity is related to specific biological processes while soil microbial respiration basically depends on the general activity of soil microbial population. It could be concluded that amount of organic matter, soil N, bulk density, porosity, MWD, soil respiration and enzymes activities are suitable indicators for soil quality evaluation in this area.

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  Soil erosion is one of the most threatening issues for crop production and environmental qualities, especially for soil and water resources. Appropriate knowledge about total soil loss and runoff is valuable in order to perform soil and water conservation practices in watersheds. EUROSEM, "a single event, dynamic and distributed model," was developed to simulate soil loss, sediment transportation and deposition by rill and interrill processes. This study was conducted to evaluate EUROSEM model in order to simulate soil loss and runoff in Sulijan sub-basin, which covered 20 ha, from Charmah-Bakhtari province. The sub-basin was divided in to 19 homogeneous elements using topographic, land use, plant cover, slope and channel properties throughout it. Soil, plant cover, land surface and climate characteristics were measured and evaluated by field observations and laboratory measurements. Actual soil loss and runoff for studied events were determined by direct measurement in the field. After sensitivity analysis, calibration and validation steps were carried out to simulate runoff and soil loss. The results of sensitivity analysis showed that the EUROSEM model for predicting runoff was more sensitive to hydraulic conductivity, capillary drive and initial soil moisture. On the other hand the model for predicting soil loss was more sensitive to Manning's coefficient and soil cohesion. The results showed that the EUROSEM model was able to simulate well the total runoff, peak of runoff discharge, total soil loss and time for the peak of soil loss discharge. But that could not simulate well the peak of soil loss discharge and time for the peak of runoff. Although it seems that EUROSEM is able to predict soil loss and runoff partially well in individual events, it is necessary to evaluate the efficiency of the models for different basins with varieties of soil, plant cover and climatic properties.

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  Extension of cultivation areas becomes gradually impossible due to ever-increasing population growth and urban area development in Iran. Therefore, it is very important to use the existing cultivated lands more efficiently. Land suitability evaluation makes the sustainable use of the lands feasible. The objective of this study was qualitative, quantitative and economic suitability evaluation of irrigated croplands for wheat, maize and sesame in Mehran plain, Ilam Province. Soil survey in the field, laboratory analysis of the soil samples, qualitative, quantitative and economic evaluation were different successive stages of this research. In qualitative evaluation, climatic, topographic and soil suitability classes were determined according to the degree of the matching with plant requirements, by parametric (square root) method. Quantitative and economic evaluations were done based on observed, potential and marginal yield analysis. Results of the qualitative land evaluation showed that most of the land units were classified moderately suitable for given crops because of soil limitations. Qualitatively, most of the land units were classified in the same classes as, or in lower classes than quantitative suitability classes for wheat and maize production, due to high management level at the farms. Whereas quantitative classes of sesame were determined lower than qualitative classes induced by low management level for this crop. Economic land suitability classification showed that the wheat production was the most economic land utilization type. Results of the economic assessment suggested that the cultivation of wheat in rotation with sesame would produce the most income for different units and could be increased in future using improvement in management level in the study area for sesame cultivation.

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Chitosan, a deacetylated derivative of chitin, is found in crustacean, arthropod, shell fish and shrimp shell as well as cell walls of some fungi . The objective of this research was to investigate the antimicrobial property of chitosan and possibility of its use as a natural preservative in mayonnaise. In this study, chitosan was produced by chemical method from shrimp shell. The antimicrobial activities of chitosan against Salmonella enteritidis and Lactobacillus plantarum were explored by calculation of the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in media supplemented with 0.1, 0.2 , 0.3, ..., 0.9 , 1 mg/ml chitosan adjusted to pH 5 or 6 . The MIC and MBC of chitosan differed with pH and bacterial type, ranging from 0.4 to 1 mg/ml. Antibacterial activity of chitosan was inversely affected by pH, with higher activity at lower pH value. The sauce containing 0.1, 0.2 or 0.3% chitosan was inoculated with 10⁶ CFU/gr S. enteritidis or L. plantarum and stored at 5°C and 25°C for 8 days. The inhibitory effect of chitosan concentration was increased from 0.1 to 0.3%. This investigation demonstrated that 0.2% chitosan can be used in mayonnaise as a new preservative.

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Determination of landscape evolution is useful to well understand the physical environment and it also enables us to conduct the soil related studies. The objective of this study was to establish the historic evolution of Zayandeh-rud Valley from late Tertiary to Quaternary. To achieve this objective, the spatial structure of a representative area of Zayandeh-rud Valley in three dimensions was examined. Responsible geologic and geomorphic processes of landscape formation were determined and inherited records and evidences of changes in soil development were investigated. The inherited foot marks and different analyses proved that following the Zayandeh-rud River formation, from Miocene to present time, the following processes and events have occurred during valley formation pathway: 1- formation of old gypsiferous gravelly alluviums, 2- lagoon formation, the change in the river pathway, 3- playa formation, 4- river terrace deposition and 5- starting of wind erosion.

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Despite the existence of highly silty soils in southern Mashhad, there is no information about the aeolian and /or in situ formation of these soils. The main objective of this study was to determine the source of silt generation in this area. Granitic hilly lands in southern Mashhad have been covered by silty deposits. Based on the soil origin, four profiles including a residual soil covered by a silty layer, a residual soil with low amount of silt, a highly silty soil and an alluvial soil as well as a deep profile containing a succession of silty and alluvial materials were studied. Cumulative particle size distribution curve (CPSDC), depth distribution curves of silt/sand ratio (Si/S), Folk inclusive graphic standard deviation, Folk inclusive graphic skewness (SKI) and sand grain morphology analyzed by scanning electron microscopy (SEM) were determined and used to identify the source of the silty materials. Based on the results obtained, silty (L), residual-silty (R-L), residual (R), alluvial (A) and alluvial-silty (A-L) horizons were identified. CPSDC of L horizons is sigmoidal in shape and is easily distinguishable from that of the other horizons. In contrast, the CPSDC of alluvial and residual horizons is spherical in shape. CPSDCs for R-L and A-L horizons are neither sigmoidal nor spherical, but something in between. The L horizons have the highest SKI (very skewed to fine particles) and the lowest Folk inclusive graphic standard deviation (very badly sorted). In this regard, alluvial and residual horizons are intermediate. Because of the silt addition to R-L and A-L horizons, these horizons have a nearly zero SKI (symmetrical) and the highest Folk inclusive graphic standard deviation (very badly sorted). Particle size distribution histograms of R-L and A-L horizons are bimodal, a mode for sand and a mode for silt, suggesting two different sources. Depth distribution of Si/S, SKI and Folk inclusive graphic standard deviation of highly silty and other horizonz show a drastic change between L horizons and the other horizons-an indication of lithologic discontinuity and difference in origin. In conclusion, despite the possible contribution of granitic parent rocks to silt generation in the area, loess deposits recognized appear to have mostly been transported by aeolian movement.

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During recent years, several genotypes have been selected from Isfahan land race Kouseh. The performance of safflower selected genotypes was studied in the field conditions, at Agriculture Research Station, Isfahan University of Technology during 2003. The experiment was carried out using a split plot arrangement according to a randomized complete block design with three replications. Planting dates (March 11, April 20, May 22 and June 23) were considered as the main plots and subplots consisted of 20 genotypes of safflower including 19 selections from Kouseh and Arak-2811 genotype. Days from planting to emergence and emergence to stem elongation were reduced as planting was delayed until the fourth planting date. Days from stem elongation to head visible was reduced with delay from the first to the third planting date, and then increased. Days from head visible to 50% flowering was reduced with delay from the first date to the third planting date, but increased in the fourth planting date. Days from 50 percent flowering to physiological maturity was increased with delay in planting. Days from planting to emergence, emergence to stem elongation, stem elongation to head visible and 50 percent flowering to physiological maturity were not influenced by genotypes. Genotype DP7 had the highest and genotype C111 and genotype DP25 had the lowest days from head visible to 50% flowering. The interaction between planting date and genotype in regard to days from stem elongation to head visible was also significant. Seed weight per plant was reduced with delay in planting date. Genotype ISF66 had the highest and genotype Arak-2811 had lowest seed weight per plant. The number of days from emergence to head visible in ISF66, DP5, C128 and Arak-2811 was affected by maximum temperature (Tmax), in genotype DP7 by Tmax², in genotypes DP17, DP1 and C41100 by Tmin² and in genotypes DP9, DP25, ISF28, ISF22, and C111 was affected by day length. It seems that development period from emergence to head visible was affected by temperature in the most genotypes. On March 11 planting date, genotypes had the maximum response to temperature and day length and the minimum response was observed in the fourth planting date. The genotype ISF66 had the highest seed yield on March 11 planting date. The result of this study showed that safflower should be planted in late March under condition similar to this experiment for maximum yield production.

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Safflower having oil with high unsaturated fatty acids is a very valuable plant. However the sensitivity of safflower to some pests as safflower fly (Acanthiophilus helianthi), particularly in drought stress conditions has limited its production. In order to evaluate the effect of drought stress on population density and damage of important safflower pests, especially safflower fly, an experiment was conducted in the field using 5 irrigation treatments including 50, 70, 90, 110 and 130 mm evaporation from class A pan in a randomized complete block design with three replications. The local safflower variety used in this study was Isfahan land variety, Kooseh. In each irrigation regime, the half of one plot was locally sprayed by Chlorpyrifos in 2ml per liter of water to be compared with non-sprayed part. During 8 weeks of sampling, population of aphids, Uroleucon carthami, leafhopper, Empoasca decipiens and safflower fly, A. helianthi and also percentage of damaged heads by safflower fly were measured. The results showed that in non-sprayed conditions, the highest safflower fly (2.38 flies per net) and aphid population (165.57 aphids per plant) was observed at 130 mm evaporation regime. Drought stress also decreased (30.23 %) safflower seed yield. Severe drought stress together with non-spaying may increase safflower pests population particularly safflower fly and reduce seed yield. However low level of drought stress (70 mm evaporation from class A pan) may decrease relative number of insects and save irrigation water. Drought stress also decrease the population of leafhopper. In this study, 70 mm evaporation regime was the optimum irrigation treatment regarding lower aphid and fly damage (15.86% damaged heads in non-sprayed condition) and higher safflower seed yield (1687.5 kg/ha in sprayed condition).

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In this research, the logging and wood extraction damages caused by wood and paper companies was investigated. The average volume per hectare of compartments 17 and 28 of Waston watershed and Compartment 7 of Alandan watershed was more than 150 cubic meters. So, these compartments were selected as the suitable research sites. The systematic randomized sampling method with 1000 m2 circular plots was used to assess the damages to residual stands and 100 m2 circular plots was used to assess the damages to regeneration. Sampling in skid trail was done with one hundred percent inventory method with a width of 12 meter. Results of this study indicated that 3.2 percent of regeneration was damaged in felling and bucking operations and 4.8 percent of regeneration was also damaged after winching and skidding operations. Most of these damages occurred for thicket stage. The felling and bucking operations damaged 13.6 percent of the residual stand. The amount of damage to the stand after skidding including the whole logging damages equals 15.5 percent. Unnecessary damages to stand and regeneration could have been avoided by selecting the best harvesting method, skidding practices, adequate incentives/disincentives and appropriate supervision.