JWSS - Journal of Water and Soil Science

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Due to physiography and weak structure, the pasture soils in Boroojen are potentially degradable. Converting pastures to agricultural land accelerates the degradation processes. A study was conducted in 1999 to show the effects of almost 20 years of farming on originally pasture land on soil physical properties, fertility, and tilth index of pastures in Boroojen region in Chahar Mahal and Bakhtiari Province (central Zagrous). Soil texture, clay content, bulk density, organic matter, saturation moisture percent, cone index, plasticity index, mean weight diameter and aggregate size and distribution, nitrogen, phosphorus, and potassium were measured.

After 20 years of cultivation, bulk density increased about 20% while organic matter decreased by 30%. Cone index was lower in the undisturbed pasture but nitrogen and phosphorus contents were higher compared to the disturbed pasture. The undisturbed pasture contained more larger (> 1 mm) aggregates, while the disturbed pasture had more smaller aggregates. Sustainable use of natural resources will lead to their long term workability, while negligence of conservational practices including appropriate farming management practices will result in the destruction of these resources.

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A Complete Randomized Block design was used to study the total protein efficiency (TPE) in Iranian Kilka and Peruvian Anchovy fish meals. Six isoenergetic (3200 kcal ME/kg) and isonitrogenous (18.5% CP) diets based on corn with six samples of fish meal (No. 1, 5, 6 steam processed, 2, 4 flame processed Kilka fish meal and No. 3 Peruvian Anchovy fish meal) were formulated and fed to Lohman male chicks from 7 to 42 days of age. Feed consumption and weight gain were measured weekly. Total protein efficiency and feed conversion (FC) were calculated and statistically analyzed.

The highest TPE values in fish meals obtained over the period of 7-28 days and significantly differed (P< 5%) among Kilka and Peruvian fish meals. With the exception of sample No. 5, the highest values of TPE obtained in the case of steam processed and the lowest obtained the case of flame processed Kilka fish meals. The TPE value of Peruvian fish meal was approximately equal to the average of the two types of processed Kilka fish meals. The orthogonal and nonorthogonal comparison of TPE and feed conversion (FC) in Kilka fish meals, regardless of the type of processing, with the same values in Peruvian Anchovy fish meal did not show any significant differences (P> 5%). With the exception of sample No. 5, the highest final weight of chicks obtained with steam processed Kilka fish meal. In general, TPE values in Kilka fish meals were variable when compared to Peruvian fish meal and TPE of steam processed Kilka fish meal was better.

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The emitter discharge is affected by parameters such as pressure, irrigation water temperature, manufacturer’s coefficient of variation, and emitter clogging. In order to study the effects of irrigation water temperature on emitter discharge and to determine the discharge-pressure variations and manufacturer’s coefficient of variations, an experimental trickle irrigation system was designed and studied in the greenhouse, College of Agriculture, Isfahan University of Technology. Since the sensitivity of different emitters to water temperature variations is variable, four different, widely used, types of Iranian emitters including in-line long-path emitter, gun emitter, pressure compensating emitter, and double-chamber tube were used. Each emitter was evaluated under 14 different irrigation water temperature treatments ranging from 11 to 43.5°C.

 The results showed that in general as water temperature increases, the emitter discharge increases linearly in all types, except for the pressure compensating emitter in which the discharge decreases linearly. The emitter uniformity, absolute emission uniformity and Christiansen uniformity coefficient parameters were affected by emitter discharge variations. For each type of emitter under study, the changes in manufacturer’s coefficient of variations due to irrigation water temperature were different. The manufacturer’s coefficients of variations at a water temperature of about 20°C for double-chamber tube, in-line long-path emitter, gun emitter and pressure compensating emitter were equal to 5, 7, 13 and 22 percent, respectively. Moving from gun emitter to the double-chamber tube and finally to the in-line long-path emitter, the sensitivity to irrigation water temperature decreased. Sensitivity to pressure variations decreased in the order of gun emitter, double-chamber tube, in-line long-path emitter, and finally pressure compensating emitter.

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Side channel spillways have a common usage in conveyance and distribution networks, high dams, water and wastewater treatment plants, and surface drainage networks. A side channel carries spatially varied flow with increasing discharge and their water surface profiles is a main feature in the design process. Usually, the bottom width of the channel is flared in the flow direction and an end sill is also installed at the downstream end to provide a control section and to generate an even water surface profile. In this study, the impact of installing an end sill on the flow characteristics in a non-prismatic side channel is presented. Six distinct longitudinal profiles were clearly observed in each run and the difference between the mid points of the maximum and the minimum profiles of each run was used to evaluate the sill effects on the water surface profile and the energy dissipation. The results indicated that the maximum and the minimum differences are, respectively, equal to critical depth and half of it generated at the channel downstream end. Also, based on an envelope of the data, a method was proposed to determine the maximum potential impact of an end sill that might have on the flow depth, which could also be considered as a guideline in the design process.

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For the study of field wind erosion and the design and evaluation of wind erosion control techniques, detailed observations of soil particle transport and vertical destribution of eroded soil particles are needed. The objectives of this study were: 1) To describe one device for soil transport particle measurement, i. e. the BSNE sediment catcher and 2) To assess vertical distribution of wind–eroded sediment with height in eastern Isfahan. The BSNE sediment catcher is a wind erosion sampler that traps eroded material at seven heights of 0.24, 0.60, 1.08, 2.00, 3.00, and 4.00 m above the soil surface. Each trap consists of a steel container with an inlet and outlet, mounted on a wind vane that rotates about a central pole. Before using the sampler in the field, it was tested and calibrated in the wind tunnel. The results showed that the average trapping efficiency with speeds ranging from 5.2 to 7.2 m sec^-1 for 4 different wind–eroded sediments was 0.44 to 0.68. However the trapping efficiency depended on wind speed, particle size distribution, particle density and type of sediment. The sampler had the lowest efficiency for particles < 44 μm. A BSNE sediment catcher was installed in Babaii Air Base. After a sampling period, the sediment in each trap was collected and weighed. The trapped materials were a mixture of saltation and suspension particles. Vertical distribution of wind–eroded sediment showed that the amount of soil collected decreased with increased height and the percentage of fine particles (<63μm) increased with height. The amount of trapped materials for each cm2 frontal intake with increased height were 12.00, 3.42, 1.44, 1.56, 0.75, 0.21, and 0.39 g cm-2, respectively, for the one sampling period.

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A total of forty-three isolates of Ascochyta rabiei obtained from infected seeds and plants of chickpea from different areas of Kermanshah, Lorestan, Hamadan, Kordestan, and East and West Azarbaijan provinces were studied during the period 1996-1999. A rapid and simple method was developed for pathogenicity test in CDA (chickpea seed meal dextrose agar) medium. CDA medium was inoculated with different isolates and incubated at 21±1 ^oC for 9 days. The disease severity was scored as 0-100% at 24-hour intervals up to five days. Virulence forms were designated according to spectrum of disease reactions of each isolate. Thirteen isolates (IK04, IK08, IK10, IK13, IK14, IK17, IK19, IK21, IL01, IL10, IO00, IE04, IE06) were distinguished as highly virulent (HV) and 7 isolates (IK03, IK06, IK15, IE01, IE08, IL04, IL06) as weakly virulent (WV). Polygalacturonase activity of these isolates from 6 days old inoculated PZ (pectin zymogram) medium were assayed. Comparison of the results of pathogenicity test and PG activity of 43 isolates demonstrated that HV isolates show predominant enzyme activity while the WV isolates show enzyme activity.

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Planting density, through its impact on the level of available environmental factors may have significant impacts on grain yield in safflower (Carthamus tinctorius L.). In order to investigate the impacts of plant density on grain yield, yield components and growth characteristics of safflower, a randomized complete block design field experiment with four replicates was conducted in spring-summer, 2000, at Lavark Research Farm (Lat. 32⁰ 32^, N and Long. 51⁰ 23^, E), College of Agriculture, Isfahan University of Technology, Isfahan, Iran. Arak-2811 and Kouseh safflower genotypes were seeded at 16.6, 22.2, 33.3 and 50 plants/m². For establishing these plant densities, plants were seeded in 12, 9, 6 and 4 cm distances, respectively, on ten 8-m-long rows spaced 50 cm apart in each plot. Plant density had no impact on plant growth stages with the exception of button formation. Genotype had significant effects on days to seedling emergence, button formation, and 50% flowering. While both number of days and accumulated growing degree-days for all growth stages decreased with an increase in plant density, Arak-2811 was earlier than Kouseh for most growth stages. Number of branches and heads per plant, number of heads per branch, number of seeds per head and harvest index showed significant decreases with increasing plant density. The decrease in the mentioned grain yield attributes was mainly negated by greater number of plants per m², leading to no significant variation in grain yield between plant densities of the two genotypes. Arak-2811 produced a significantly greater number of heads per branch and 1000-grain weight however, these differences did not lead to any greater grain yield compared to Kousehdue, mainly due to the greater number of branches in the latter. Neither of the leaf area index, leaf area duration, and crop growth rate varied significantly with plant density and between two genotypes, suggesting no difference in dry matter production capabilities of the two genotypes under environmental conditions of the present study.

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In this study, susceptibility of the first three larval stages of Spodoptera exigua to Bacillus thuringiensis was investigated. To determine the LC₅₀ values for each larval instar, 6 different logarithmic concentrations of Bacillus thuringiensis were used. A control was also included in each of the bioassays. The value of LC₅₀ for 1st instar larvae, that had been treated one to four hours after hatching, was 311.617 ppm. For the 2^nd and third instars, the larvae values of LC₅₀ were 1356.95 and 2708.27 ppm, respectively. It was clear that larval susceptibility to Bacillus thuringiensis decreased with age. In order to enhance the Bacillus thuringiensis efficiency to control Spodoptera exigua under field conditions, Bacillus thuringiensis should be applied at egg hatch peaks.

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Greenhouse whitefly, Trialeurodes vaporariorum West. is one of the most important pests of the field crops and ornamental plants in greenhouses. Pest control is based on using chemical insecticides and biological control agents. In this study, the efficiency of the green lacewing, Chrysoperla carnea ( Steph.) with release ratios of 1:1, 1:5, 1:10, 1:15, and 1:20 (predator:prey) using eggs in chrysobag and the effect of three insecticides including Confidor (35% Sc), Permethrin (25% Ec) and Dimilin (25% Wp) at concentrations of 0.3 ml/l, 0.3 ml/l and 0.15 g/l in water, respectively, were investigated. In one experiment, the eggs of the green lacewing were released on tomato plants that were infested with 150 different nymphal instars of the pest. Control treatment included chrysobag whithout any egg. After 11 days, nymphal mortality was calculated. In insecticidal experiments, in control treatment, only water was sprayed. Both experiments were conducted in a randomized complete block design with four replications in a greenhouse with 25±5 °C, 50-60% RH and 14:10 LD period light conditions. The means of data were compared using Duncan Multiple Range Test. The results showed that the maximum nymphal mortality of 52.42% was due to the release ratio of 1:1 (predator:prey) however, this was not sufficient and did not control the pest population. The results of the chemical control also revealed that one application of Confidor caused the highest mortality about 88% that was more effective in reducing pest population compared with the release of predator eggs. Neither method had absolute control. In general, results indicated that to be more effective the two methods need to be applied more than once, and they must be combined into one or with other control methods.

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Watermelon (Citrullus Lanatus) cv. Charleston Gray is one of the most important cultivars grown in Iran. It has some good quantitative and qualitative characteristics but unfortunately is sensitive to Blossom End rot. To solve this problem, this experiment was conducted in a factorial manner in randomized complete block design with four replications in Research Station and Laboratories of Department of Horticulture, College of Agriculture, the University of Tehran, during 1997 & 1998. In this experiment, black polyethylene mulch was used and calcium nitrate was foliar sprayed at concentrations of 0, 4, and 6 g/L. Results indicated that mulch could increase yield by 85% over two years due to weed growth suppression and conserving soil moisture for a longer time. Foliage fresh weight, number and average weight of fruits per plant and precocity were also significantly affected by black polyethylene mulch. Furthermore, mulch reduced the number and weight of fruits affected by Blossom End rot by about 13% and 12.5% (average of two years), respectively. Calcium nitrate addition during both years had no significant effect on the measured fruit characteristics nor on Blossom End rot.

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Russian Wheat Aphid (RWA) is a serious pest of wheat, barely and other small grains in cereal-producing regions of the world. The use of resistant cultivars is an effective management strategy to control this pest. In this study, 15 lines and 13 cultivars of durum and bread wheats along with two resistant and susceptible controls were used to evaluate their reaction to RWA under greenhouse conditions, using completely randomized design with four replications. Three seedlings at each plot were considered as an experimental unit. Five aphid nymphs (4-5 ages) were placed on each plant at 1-2 leaf stage. Leaf rolling and leaf chlorosis percentages were measured 21 days after infesting and used to screen the genotypes. Despite the relatively high linear correlation between two resistance components, leaf rolling and leaf chlorosis, estimated as 80%, analysis of variance indicated significant differences among genotypes. Eight durum lines DW9, DW14, DW4, DW2, DW7, DW8, DW12 and DW3 and one bread wheat cultivar (Azadi) were significantly more resistant than the resistant control (Halt). One durum line (DW13) and two bread wheat cultivars (Shiroodi and M-75-7) had higher leaf rolling and leaf chlorosis than the susceptible control (Sholeh). So they were selected as the most susceptible to the biotype of D. noxia. under study. Orthogonal comparison showed that durum lines were more resistant than bread wheat cultivars. These results were confirmed by Cluster Analysis based on leaf rolling and leaf chlorosis.

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Despite its economic importance, not much information exists on growth and productivity of durum wheat in Iran and Isfahan. In order to evaluate growth, grain yield, and attributes in three durum wheat genotypes, Osta/Gata, Dipper-6, and PI40100, with five planting densities of 200, 275, 350, 425 and 500 seeds/m², a four-replicate RCBD field study with a spilt-plot layout was carried out in winter 2000 to spring 2001 at Lavark Research Farm, College of Agriculture, Isfahan University of Technology. Genotypes were the main-plots and planting densities, the sub-plots. Each plot contained six rows, spaced 25cm apart, each 6m in length. While Osta/Gata produced a greater spikes/m², 1000-grain weight and grain yield, PI40100 indicated a greater height, leaf area index (LAI) and biological yield, and Dipper-6 produced a larger number of grains per spike and harvest index, compared to the other two genotypes. Increasing plant density led to an increase in LAI, spikes/m², grain yield, biological yield and harvest index but a decrease in grains/spike and 1000-grain weight. Grain yield was positively correlated with spikes/m², grains/spike and 1000-grain weight, despite negative correlations with LAI and plant height. It may be concluded that with environmental conditions such as those encountered in Isfahan in 2000-2001, Osta/Gata can be planted at approximately 425 seeds/m², if an acceptable grain yield is to be achieved.

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To study the genetic diversity in 100 genotypes of rice, an experiment was conducted at the research farm of Rice Research Institute of Iran. The experimental design was a 10x10 simple lattice. The genotypes, mostly belonging to Isfahan Province and north of Iran, were evaluated on the basis of morphological traits and yield components. The results of analysis of variance demonstrated that the differences among genotypes were highly significant (p < 0/01) for all traits. High values of phenotypic and genotypic coefficients of variation were obtained for most traits, indicating high variability in the traits under study. Factor analysis revealed three factors which determined 90 percent of yeild variation and were named “grain number”, “plant type and structure” and “grain shape”, respectively. Cluster analysis by “Cubic Clustering Criterion” and “Pseudo Hotelling T² Test” grouped genotypes in four clusters. Analysis of variance showed that the differences among clusters were highly significant for most traits.

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The host-stage preferences of alfalfa black aphid, Aphis craccivora, by parasitoid wasp, Lysiphlebus fabarum, were studied under two trials under growth chamber conditions (55±5 %, R. H., L:D 14:10 H. and Tem. 20 ±2^° C ). In the first trial, all stages of aphid in equal ratios, and in the second trial, variable ratios of 5:15, 15:15 and 15:5 of 3rd and 4th instar nymphs were used for parasitisation. The objective was to identify the most preferred stage of aphid for parasitism by Lysiphlebus fabarum. The results showed that 3^rd instar nymphs of alfalfa black aphid were the most preferred stage with 38.75% of parasitism. Whereas 4^th instar nymphs and adults had 23.75% and 21.25% parasitism, respectively. The first instar nymphs were not parasitized at all. In the second trial with variable ratios of 3^rd and 4^th instars of alfalfa black aphid, parasitism of 3rd instar in ratios 25, 50 and 75 percent of total population were 35.9, 55.74 and 79.6%, respectively, while those for 4^th instar nymphs were 20.36, 44.26 and 64.09%, respectively. So there was a positive preference for 3^rd instar and a negative preference for 4th instar nymphs.

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Clay minerals deserve special attention as they play a crucial role in many soils. The clay mineralogy of five gypsiferous soils from different landforms in eastern Isfahan was investigated using X-ray diffraction (XRD). In addition, soil aggregates and wind-deposited sediments were examined by scanning electron microscope (SEM) and analyzed by energy dispersive X-ray analyzer (EDX). The results indicate the presence of palygorskite, mica, kaolinite, chlorite, and quartz with a trace amount of vermiculite and randomly interstratified layers in all soils. Smectite occurs in soils of both the piedmont plain and old river terrace, but not in the alluvial fan soils. Mica, chlorite, quartz, and kaolinite were probably inherited from the parent material. Palygorskite seems to increase with depth in the alluvial fan, whereas, in the old terrace soils, this clay mineral decreases with depth. Palygorskite present in alluvial fan soil appears to have been formed authigenically when the basin was covered with shallow hyper-saline lagoons toward the end of the Tertiary. Palygorskite in the old terrace seems to be mostly detrital and an eolian origin of palygorskite is likely because a large amount of palygorskite is present in upper soil horizons. A higher proportion of smectite in deep soils of the old terrace, as compared with palygorskite, suggests the possibility of authigenic formation of smectite from palygorskite. .

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Systemic properties of pyroquilon, an anti-blast systemic chemical, used in controlling rice blast disease caused by Pyricularica grisea, was investigated through in vivo tests. Rice plants were treated with pyroquilon as granules in soil. Leaf and stem tissues were collected at different times after treatment and pyroquilon was extracted from tissues and detected by gas chromatography. Treated rice plants were also exposed to disease infection in blast nursery at different times and disease incidence was evaluated one week later. Pyroquilon was detected in leaf tissue one day after treatment (DAT), reached maximum during 4-7 DAT, and then reduced gradually to a nondetectable level after 28 DAT. In stem tissue, change in pyroquilon content was similar to but its quantity was less than that of leaf tissue. On inoculated plants, disease was controlled up to 60% at 2 DAT and completely controlled during 4-28 DAT. The results indicated that pyroquilon could be taken up and systemically translocated in rice plants after applying as granules in soil. Extent of disease control was a good function of pyroquilon content in leaf tissue (R²=0.93) and 2 ppm of compound in leaf could provide 90% control of the disease. In contrast, disease control was not a good function of pyroquilon content in stem tissue (R²=0.30).

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In order to identify the plant-parasitic nematodes of important cereals (wheat, barley, and corn) in Isfahan Province, 120 soil and root samples were collected from the rhizosphere of these crops in various locations in the region during 1999 and 2000. Nematodes were extracted from soil by sieving and centrifugal sugar-flotation method. The specimens were killed and fixed in FGA (formaldehyde, glycerin and acetic acid 4:1:1) hot solution. The extracted nematodes were mounted in glycerin, permanent slides prepared, and studied by light microscope. The results revealed the presence of three species belonging to two genera of family criconematidae as follows: Criconemella antipolitana , C. xenoplax, Hemicycliophora poranga. H. poranga was extracted from rhizosphere of corn and reported for the first time from Iran. The most frequently occurring and dominant species was C. antipolitana that was isolated from soil around the roots of wheat and barley in several areas. C. xenoplax was extracted from a wheat field with the history of peach cultivation. These species are new records for Isfahan Province.

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A great number of studies have been conducted on the role of Azotobacter - a free living N₂-fixer - in agricultural soils, but our information is lacking on the importance of Azotobacter in pasture soils. This research was conducted to study the relationship between ecological and edaphical factors with the population of the bacteria in soil. Soil samples were taken from two sites in Azarbaidjan (Mishou-dagh and Khadjeh), having different ecology, soils and vegetation types. We investigated also the effect of inoculation of rhizosphere with soil-isolated A. chroococcum on the growth of wheat (Triticum aestivum L. cv. Omid) during 56 days of growth in a pot experiment. The treatments included: 1) inoculation with A. chroococcum (+A), 2) N fertilizer at a rate of 35 mg/Kg as (NH₄)₂SO₄ (+N), 3) +A+N and 4) –A–N as control with four replications. Results showed that the population of Azotobacter chroococcum correlated positively with organic carbon and negatively with soil pH. In soils covered with vegetation the population of bacteria was higher than that in uncovered soils and the highest population of bacteria was found in soil samples collected from the rhizosphere of grass species and the lowest in that of legume species. In inoculation experiment, shoot and, particularly root growth, increased in the order of +N, +A and +A+N in comparison with control (–A–N) plants. These treatments also stimulate uptake and transport of K into shoot. Interestingly, the effect of +A treatment was significantly higher than that of +N and +A+N treatments. The same results were obtained for nitrogen. A higher uptake and particularly transport of K into shoot was attributed to low molecular weight organic molecules produced in inoculated roots and transport K into shoot. Results showed that inoculation with Azotobacter not only enhanced growth and chlorophyll content, but also stimulated uptake and translocation of ions into shoot. The stimulatory impact of inoculation is most likely a general effect that could also be important in micronutrients translocation into shoot.

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Analysis of transgenic plants is very important in gene transfer programs. In this research, the second generation (T₁) of transgenic brassica napus which was transformed by antisense of Glutamine synthetase (GS) gene was studied from the view of total soluble protein content of leaf, total chlorophyll and protein patterns (SDS-PAGE) using seeds of Brassica napus .Protein concentration was determined by a calorimetric method described by Bradford method. Chlorophyll (a, b) and carotenoid contents were determined by spectrophotometry. The total soluble protein content of Brassica napus leaves increased from YG stage, reached a maximum level during MG₂ and, after this, decreased with the progress of SS stage. Comparison of the total soluble protein between different treatments showed the highest level in the A₂ plant and the least in the A₆ plant. Comparisons with chlorophyll a and b were not significant between different treatments, but different stages showed significant differences with maximum and minimum levels obtained in the MG₁ and SS, respectively. Protein patterns were also studied using SDS-PAGE method. No new band was recognized in the MG₁ stage, but the density of some protein patterns was shown in YG and MG₂ stages. In YG stage, differences were seen among the A₅, A₃, A₄ and A₆ with W.T on 41 KDa weighty position and also between the A₁ and A₂ with W.T treatment on 23/6 KDa. Considering the molecular weight GS₁ subunit of glutamine synthetase (41 KDa) and the similar conditions in growth, protein extraction and macro molecular analysis in transgenic and control plants, we can tell that the difference shown in transgenic plants are probably due to the effect of the antisense of glutamine synthesis gene in this plant.

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The coccinellid Cryptolaemus montrouzieri Muls. is an oligophagous predator that is used to control mealy bugs in the orchards and greenhouses. Citrus soft scale, Pulvinaria aurantii, as an active pest on citrus plants in North of Iran is also attacked by this predator. This research was conducted to study biological characteristics of C. montrouzieri on citrus soft scale and mealy bug, Planococcus citri. Initially, Cryptolaemus eggs were collected from colonies of two preys and reared in laboratory (14 L: 10 D, 25±1˚C, and RH 70 ±5%). The incubation period of eggs, 1^st, 2^nd, 3^rd and, 4^th larval instars, and total larval, prepupal, and pupal stages were studied on two preys. The developmental stages on two preys were not significantly different, except for the 4^th larval instar at 1% and total larval stages at 5%. The sex ratio of male to female on two preys were not significantly different at 1% and was 1:1. Except for pre-oviposition period, other parameters including female longevity, average number of eggs laid per day, average total number of eggs laid per female and percentage of hatching were significantly different at 1% on the two preys. The average feeding of total larval period on eggs of mealy bug and citrus soft scale were 5715 and 7694, respectively that, the difference was significant at 1%. The results showed that some of the biological characteristics of Cryptolaemus on two preys were significantly different. Since the predator feeding on the eggs of citrus soft scale was higher than the mealy bug, it seems that this beneficial insect could be used for biological control of the citrus soft scale.