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Showing 425 results for Oil

M. Shafaei Bajestan, M. Salimi Golshaikhi,
Volume 6, Issue 4 (1-2003)
Abstract

Downslope soil movement along riverbanks is a significant erosion process. Plant roots, particularly of woody vegetation, apparently stabilize soil on slopes because in most areas where the vegetation is removed, frequent bank failure occurs. Plant roots increase soil-shearing resistance both directly by mechanical reinforcement and indirectly through removal of pore water by transpiration. In this study, the effects of two plant species on the stability of the Karoon River has been investigated. To determine the In-situ shear strength of soil, a special device was designed and manufactured. This device is capable of measuring the shear strength of soil blocks as large as two cubic meters. In this study, twelve soil blocks, four blocks with roots of each tree and four blocks of root permitted were measured. Comparison of the soil shear strength with roots and root permitted soil shows that tree roots can significantly increase the shear strength of the soil. The amount of increase depends on the type of plant, the age of plant, the diameter of the roots and the percentage of roots in the block. In this study, the amount of increase varied between 20-66%. From the analysis of the data, two equations were developed to determine the increased shear strength.
A. Ahmadi Iikhchi, M. A. Hajabbassi, A. Jalalian,
Volume 6, Issue 4 (1-2003)
Abstract

Cultivating rangeland to be shifted to crop land farms commonly causes soil degradation and runoff generation. This study was conducted to evaluate the cultivation effects on runoff generation and soil quality. The experiment was performed in a rangeland and a 40-year cultivated land located at two slope positions (back slope and shoulder) of a hillside in Dorahan, Chaharmahal & Bakhtiari Province. A 60±5 mm.hr-1 rainfall intensity was simulated by a rainulator. Organic matter, mean weighted diameter, saturated hydraulic conductivity, collected runoff and sediments were measured. The differences between the means were tested using T-test. Results showed 35, 53 and 8% increases in the organic matter, mean weighted diameter, and saturated hydraulic conductivity in back slope, respectively. The increases in these parameters in shoulder position were 39, 60 and 33%. The values for runoff and sediments in back slope were 3 and 8 times greater than in other similar positions while the values in the shoulder position were 11 and 55 times greater than the same values in other positions.
K. Yussefi Kelaricolaii, A. Kamyab, M. Rezaei,
Volume 6, Issue 4 (1-2003)
Abstract

In a completely randomized design (CRD) experiment with 6 treatments and 4 replicates per treatment, the effect of energy and protein dilution during 6-12 days of age was tested in broilers. Rice hull was used for dilution of energy and protein at levels of 0.05 (control), 10, 15, 20, 25 and 30 percent. The metabolizable energy and crude protein content of the experimental diets were 3089, 2780, 2626, 2472, 2317 and 2163 KCal/kg and 21.45, 19.29, 18.23, 17.16, 16.10 and 15.02 percent, respectively. The results indicated that diet dilution at early age (6-12 days) increased feed consumption but energy and protein intake and weight gain were reduced. No significant differences in body weight were observed due to compensatory growth among treatments. After feed restriction, feed intake was lower for the birds receiving diluted diets as compared to control. Meanwhile feed efficiency somewhat improved among the birds fed with diluted diets, but the differences were not significant. Diet dilution had no significant effect on carcass and abdominal fat percentage at the slaughter age (45 days). Diet dilution with rice hull at 20 and 25 percent decreased feed consumption and improved feed efficiency and, therefore, its use can reduce feed cost.
A. Mojiri, A. Arzani,
Volume 7, Issue 2 (7-2003)
Abstract

In order to study the effects of different levels of nitrogen fertilizer and plant density on grain yield and its components in sunflower, an experiment was conducted using 'Record' cultivar at the Research Farm of College of Agriculture, Isfahan University of Technology in 1996. Four levels of nitrogen (0, 75, 150 and 225 kg/ha) and four plant densities (65000, 75000, 85000 and 95000 plants/ha) were used in a split plot arranged in a randomized complete block design with three replications. Developmental stages, plant height, stem diameter, head diameter, number of head per m2, grain yield, biological yield, harvest index, 1000-grain weight, number of grains per head, grain oil percentage, oil yield and grain protein content were measured. The results indicated that N fertilizer caused an extension of the growth period and means of days to physiological maturity. It also increased plant height, stem diameter and head diameter. While increasing plant density had an incremental effect on plant height, it negatively affected stem diameter and head diameter. N fertilizer up to 150 kg/ha increased the grain yield and biological yield, whereas higher levels of N fertilizer decreased both. Plant density of 85000 plants per hectare was observed as a suitable plant density, whereas the higher plant density had a negative effect on grain yield. N fertilizer via increasing the number of grains per head, and plant density via increasing the number of heads per unit area and also decreasing the number of grains per head influenced the grain yield. One-thousand grain weight was not affected by neither N fertilizer nor plant density. Considering the superiority of 150 kg/ha of N fertilizer and plant density of 85000 plants/ha for grain yield and oil yield, it appears that they could be recommended for producing desirable yield in the regions similar to the study region.
M. Toghiani, A. Samie, A. Gheisari,
Volume 7, Issue 2 (7-2003)
Abstract

This experiment was conducted to investigate the effects of intensity of restricted early feed and increased nutrient density after restriction period on performance and compensatory growth in broilers. Two hundred and eighty-eight broiler (Lohman) chicks in a completely randomized design in a 2*3 factorial arrangement of treatments were employed. Birds were fully fed from 7 to 13d or 100% of adlibitum intake on a daily basis. The diets were diluted at 25% and 50% levels with rice hulls. After the restriction period, birds were fed with a diet, formulated to meet all their nutrient needs, according to the NRC (1994) or 10% of protein, methionine and lysine above NRC in the realimentation diet from 13 to 21d. Body weight, feed intake, daily gain and feed conversion were determined weekly and selected carcass characteristics (liver, intestine and abdominal fat pad) were measured at 49 days of age. Feed restriction significantly (P<0.01) decreased body weight gain and feed conversion during the restriction period. Birds were able to fully recover body weight depression up to 49d. The intensity of restriction had no effect on body weight, feed intake, daily gain and feed efficiency at 49d (p>0.05). Increased nutrient density had no effect on growth rate, feed intake and feed efficiency for fully-fed birds and restricted birds at 49d. The results from this experiment showed that broiler chicks may be expected to gain weight similar to chicks with fully feeding even after restricted early feed if fed with NRC diets. The effect may be due to compensatory growth.
A. Gheisari, R. Bahadoran, S. S. Tadayonfar,
Volume 7, Issue 2 (7-2003)
Abstract

In order to study the nutritive value of grades 1 and 2 wheat feed screening and macaroni wastes as energy resources, 360 21-day-old broiler chicks were divided into 36 groups of 10. In this experiment, each experimental levels of feedstuff was used at 0 (control group), 15, 30 and 45%. In addition, their apparent metabolizable energy (AMEn) was determined by Sibbald’s method. Daily feed intake of chicks fed by diets containing macaroni wastes and grade 1 wheat feed screening for 21 to 56 days of age was significantly lower than that in the control group (P<0.05). In contrast, body weight, daily weight gain and feed conversion had no significant differences during this period. However, in the total experimental period, the best feed conversion belonged to chicks fed with various levels of macaroni wastes and grade 1 wheat feed screening. Apparent metabolizable energy (AMEn) of macaroni wastes and wheat feed screening were 3700, 3270, and 2870 kcal/kg, respectively. According to the results of this experiment, it is possible to use macaroni wastes and grade 1 wheat feed screening at 45% and 30% levels in the diet of broiler chicks, respectively, without any undesirable effects on their performance.
S. A. M. Mirmohammady Maibody, A. Amini, J. Khajeddin,
Volume 7, Issue 2 (7-2003)
Abstract

In this study, the potential use of halophyte grasses, Aleuropus littoralis and A. lagopoides, on soil desalinization and lowering soil salinity was investigated under glasshouse conditions. The seeds of two species were collected from Rodasht area in Isfahan and grown with four salinity treatments obtained from different types of collected soils and replicated three times. Electrical conductivities of the four soil treatments were 12.4, 29.5, 43, and 69 dS/m. The results of mean comparison showed that shoot and root dry weight, sodium contents of dry weight and Na/K ratio were significantly different in both salinized species. Both species effectively reduced soil electrical conductivity by 23 to 42.5%. This was mainly due to ion absorbtion, and consequently, from Na+, Cl-, Mg2+, Ca2+ ionic reduction. In general, considerable amounts of ionic absorbtion and total soluble salt secretion through their salt glands appeared to have the most pronounced effects on decreasing soil salinity. Regarding 50% salt excretion by these species, growing these grasses could be a possible way to decrease soil salinity by grazing or harvesting salt crusted foliage from the site.
M. H. Farpour, M. K. Eghbal, H. Khademi,
Volume 7, Issue 3 (10-2003)
Abstract

Gypsiferous Aridisols are of great importance and extent in arid and semi-arid environments. There is a close relationship between soil genesis and landscape positions. This study aimed to determine the genesis and classification of gypsiferous soils and to investigate the relationship between micro-and macro-morphology of gypsum crystals and geomorphic positions in Rafsanjan area. The study area is located in Nough, 30 km north of Rafsanjan with a mean annual precipitation of 60 mm. Seven representative pedons were selected on different geomorphic positions. Physico-chemical, micromorphological, XRD, and SEM observations were performed on soil samples. Rock pediment geomorphic surfaces, that are in fact peripheries of old closed water bodies in central Iran, are the source of gypsum in the area. Large gypsum pendants and microforms of lenticular, vermiform, platy, and interlocked gypsum plates are found in rock pediment. The amount of gypsum and the size of pendants decrease moving down the slope. Lenticular and interlocked gypsum plates are found in a transition of pediment and playa. Puffy ground is observed on the saline surface of playa. Lenticular and vermiform gypsum crystals increase down the slope, but alabastrine gypsum is the most dominant form of gypsum in the playa surface. Large amounts of soft gypsum nodules are found on the rock pediment surface (western slope). In addition, spindle form of gypsum and palycrete bundles are observed in this position. Wind action played a significant role in the genesis and development of soil in rock pediment. A close relationship was found between morphology of gypsum crystals and geomorphic positions.
S. Vaseghi, M. Afyuni, H. Shariatmadari, M. Mobli,
Volume 7, Issue 3 (10-2003)
Abstract

Excessive application of sewage sludge leads to the accumulation of potentially toxic elements in soils. The objective of this greenhouse study was to examine the DTPA–extractability of Fe, Zn, Cu, Mn, Pb, Cd, Ni, and Co in relation to soil pH and to investigate the concentrations of these metals in corn (Zea mayz). The study was conducted using a factorial experiment in a completely randomized design with three replications. Sewage sludge was applied at 0, 50, 100, and 200 t/ha on four soils [Langaroud (pH= 4.8), Lahijan (pH= 5.7), Rasht (pH= 6.8), and Isfahan (pH= 7.9)]. Application of sewage sludge significantly increased DTPA-extractable Fe, Zn, Cu, Pb, Cd, and Ni in all soils. The increasing effect was in accordance with sludge level. Langaroud soil had the highest and Isfahan soil the lowest levels of DTPA-extractable metals. Application of sewage sludge increased plant growth and metals in the foliage of corn plants. The metal concentration in corn tissues also increased with decreasing soil pH. Overall, the application of sewage sludge as an organic material, particularly in acid soils, may increase availability of heavy metals, which may, in turn, result in soil pollution. Therefore, addition of sewage sludge to soils should be managed on the basis of the changes in soil heavy metal concentration.
F. Nourbakhsh, A. Jalalian, H. Shariatmadari,
Volume 7, Issue 3 (10-2003)
Abstract

Cation exchange capacity (CEC) is one of the most important chemical characteristics which influences soil quality from different aspects. At the same time, CEC is an input parameter of many computer models being applied in soil science and agriculture. Methods of CEC determination are always time-consuming and laborious. Therefore, developing a model for CEC estimation from other soil properties is essential. The objective of this study was to understand the associations between CEC (as a dependent variable) and sand, silt, clay, organic matter and pH (as independent variables). In this study 464 soil samples from A, B, and C horizons of different soils were used. Results revealed that CEC is negatively correlated with sand (r=-0.389***) and is positively correlated with organic matter (r=0.772***), clay (r= 0.391***) and silt (r= 0.233***). No significant correlation was observed between CEC and pH. Stepwise regression analysis showed that both organic matter and clay enter the model and that coefficients of determination (r2) for the multiple models are higher than those of simple linear correlations. Other parameters could not increase the r2 considerably. Correlation analysis on data from A, B, and C horizons revealed that the CEC of organic matter in different horizons are not the same. Separation of Aridisols could not increase the r2 of the model and the accuracy of the estimations. Correlation studies in acid soils showed that the contribution of organic matter in CEC is much higher than that of clays.
Sh. Mahmood-Soltani, A. Samadi,
Volume 7, Issue 3 (10-2003)
Abstract

Knowledge of forms of phosphorus (P) is important in the evaluation of soil P status and also in understanding soil genesis and fertility. Amounts and distribution of P in different organic and inorganic fractions were examined in 39 soil samples of Fars province. Soils were sequentially extracted to determine organic P and inorganic P fractions. Total P with an average of 597 mg/kg soil was lower in the light soils (482 mg/kg) as compared with the heavy soils (690 mg/kg). Organic P constituted considerable portions of TP (27%). Calcium phosphates with an average of 424 mg/kg soil and being comprised of 71% inorganic P was the dominant form of soil phosphorus. Simple regression analysis indicated that there were significant positive relationships between organic P, Ca phosphates and Al-Fe phosphate values with CEC and clay content. Stepwise multiple regression analysis showed that a combination of soil properties such as clay content and equivalent calcium carbonate (CCE) increased correlation coefficients (R). Alghough multiple regression model was significant (at P<0.05), the relative contribution of each soil property in Al-Fe-P fraction was not considerable.
G. Saeidi,
Volume 7, Issue 3 (10-2003)
Abstract

In order to investigate agronomic traits and yield potential of edible-oil flax (Linum usitatissimum L.) as a second crop in Isfahan region, different genotypes were evaluated in separate experiments in early spring (April, 7) and summer (July, 16) planting dates, using a Randomized Complete Block Design with three replications. The experiments were conducted in agricultural research farm, Isfahan University of Technology in the year 2000. The results showed that summer planting considerably and significantly reduced number of seedlings per unit area, days to 50% flowering, and seed yield. However, maturity of the plants was delayed because of summer planting. According to overall average obtained for genotypes, seed yield was 1472 and 213 kg/ha in the first and second planting dates, respectively. There was a significant difference between genotypes for number of seedlings per unit area, days to 50% flowering and maturity in both planting dates. However, genotypes were significantly different for seed yield in the first planting date. Summer planting also non-significantly reduced yield/plant, capsules/plant and 100-seed weight and increased seeds/capsule. The differences between genotypes for these traits in both planting dates and for seeds/capsule in the first planting date were significant. The significant interaction between genotypes and planting dates on seeds/capsule and 100-seed weight was because of increasing or decreasing levels of these traits in some genotypes when planting date was delayed. The results of regression analysis, correlation coefficients and path analysis showed that in both planting dates, capsules/plant followed by seeds/capsule and 100-seed weight were the major components of yield/plant. Capsules/plant had the most (approximately 80%) contribution in variation of yield/plant in both planting dates. The number of plants per unit area affected yield/plant via indirect and negative effect of capsules/plant.
A. A. Gheisari, O. Saraeian, M. Toghiani, D. Asadian,
Volume 7, Issue 3 (10-2003)
Abstract

This experiment was conducted to study the effects of using cotton-seed meal on the performance and carcass yield of broiler chickens at different ages. The experiment was performed in a completely randomized design with four treatments and five replicates for each treatment. The experimental treatments included the levels of 0, 3.5, 7, and 10.5% of cotton-seed meal in diets fed to chickens at 7-21 days of age. Except for the control group, these levels for grower (21-42 d of age) was increased to 7, 14, and 21% and for finisher (42-56 d of age) to 14, 21, and 21%, respectively. For the purposes of this study, 260 day-old chicks were reared in litter until 7 days of age. On day 7, chicks were divided into 20 pens with 13 chicks in each pen. NRC (1994) recommendation was used in balancing experimental diets. The chickens were weighed weekly throughout the experimental period (7-56 d). The main observations were body gain, feed intake and feed conversion ratio. Physical analysis of carcass was done at the end of the experimental period and percentages of carcass, abdominal fat, liver, pancreas and digestive tract to live body weight were calculated. The use of cotton-seed meal and the increase of the levels through different ages had no significant effects on body weight, daily gain, feed intake, feed conversion ratio, and carcass components. Increasing the level of cotton-seed meal in the diet at 7-21 days of age had significant effects on feed intake (P<0.05) and increased it. The results of this experiment indicate that we can use cotton-seed meal at levels of 7,14, and 21% of diets, respectively, in starter, grower and finisher stages without having any adverse effects on the performance and carcass yield of broiler chickens.
S. Maghsoud Lou, A. Golian, F. Eftekhar Shahroudi, M. Nassiri Mahallati, H. Kermanshahi,
Volume 7, Issue 3 (10-2003)
Abstract

An experiment with a 33 factorial arrangement in a completely randomized design with 450 day-old broilers was conducted to study the effect of energy level and time of change from starter to finisher diets on performance and economic aspects of broilers. Broiler chickens were fed at three levels of dietary energy (2800, 3000, and 3200 KcalME/Kg) from 1-42 days of age. Starter and finisher diets were changed at 16, 21, and 26 days of age and then finisher diets were fed up to 42 days of age. The effect of diet energy and changing time from starter to finisher diets on body weight and feed conversion at 26 days of age were significant (P<0.01). By increasing energy level and changing time from starter to finisher diets, at this age, body weight increased and feed conversion ratio decreased. Energy conversion decreased by decreasing the levels of energy content of diet and increasing time of change from starter to finisher diets (P<0.01). At 42 days of age, the effects of diet energy and changing time from starter to finisher diets did not show any significant effects on feed and energy conversion in contrast, body weight at this age increased significantly as a result of increasing time of change from starter to finisher diets (P<0.05). From 1-42 days of age, the interaction between diet energy and changing time from starter to finisher diets on feed and energy conversion was significant (P<0.05). The results from this study suggest that increasing diet energy and changing time from starter to finisher diets for producing broilers with less than 2 kg will improve their general performance.
D. Ataii, M. A. Sahari, M. Hamedi,
Volume 7, Issue 3 (10-2003)
Abstract

In this study, some physico-chemical characteristics of Iranian tea-seed oil (Lahijan variety) were compared with those of the oils from sunflower seed (Fars variety) and olive (Gilezeytoon variety) in terms of fatty acid contents, peroxide value, iodine value, saponification value, and oil shelf-life. Also the shelf-life of sunflower and olive oils containing 5% and 10% tea-seed oil were compared against control sample shelf-life. Tea-seed oil was found to be suitable and stable in nutritional properties. At a temeprature of 63oC, tea-seed and olive oils were found to have identical shelf-life values, which was higher than that of sunflower oil. Furthermore, the shelf-life of sunflower oil increased when mixed with tea-seed oil.
M. Torki, J. Arshami, C. Douglass,
Volume 7, Issue 4 (1-2004)
Abstract

To evaluate the effect of broiler growth rate and feed intake on development of the inflammatory response‚ commercial broiler diets were fed to 275 day–old chicks of the modern 2000 strain and 1957 random-bred strain. Half of the chicks had free access to feed while others were restricted. Twelve birds from each strain and group pens were removed‚ weighed, and housed in individual cages by their original feeding programs on 0‚ 7‚ 14, and 28 days. Three feed-restricted and three ad libitum birds from each strain were injected with 1-3 ml of 100 μg/ml solution of Salmonella typhimurium lipopolysaccharide on days 6‚ 13‚ 27‚ and 41, and 3 birds of each group were not injected‚ serving as controls. All birds were weighed and the liver‚ breast muscle‚ thymus gastro intestinal tract and bursa were removed on the day after injection. The thymocytes of the control birds were subjected to an interleukin–1 responsiveness assay. The 1957 strain birds were more severely affected by an inflammatory challenge going almost completely off feed‚ but the 2000 strain birds experienced a more drastic decline in growth rate after injection. Thymocytes of ad libitum birds were more sensitive to IL-1 than the restricted birds in week 4 (P=0.056). Interleukin-1 stimulated thymocytes of 2000 birds more effectively than the 1957 strain ones. In conclusion‚ the broiler inflammatory response was affected by growth rate and feed intake.
J. Poureza, M. Mohammad Alipour,
Volume 7, Issue 4 (1-2004)
Abstract

This experiment was conducted to investigate the effect of whey via drinking water on broiler chicken performance. In a completely randomized design, whey at levels of zero, 10, 20, 40, 80, and 100% of drinking water was used for 47 days. 720 day-old broilers (Ross) were divided into 24 groups, 30 chicks per group and each experimental treatment was given to four replicates from 7-54 days of age. The whey was supplied freshly. The chicks had free access to feed and water during the experimental period. All chicks were fed with starter, grower, and finisher diets. Chicks in all pens were weighed and their feed consumption was calculated at 21, 42 and, 54 days of age. At the end of the experiment, two males and two females from each pen were selected, killed and their carcass, abdominal fat, pancreas, liver and ileum were weighed. Ileal contents of each pen were collected and pooled and kept at -20oC for bacterial count. The litter moisture content of each pen was determined at days 21 and 42. Body weight, daily body weight gain, feed conversion, and litter moisture were significantly (P<0.01) affected by whey levels. These indices decreased as a result of consuming whey by more than 40% feed conversion, however, increased. Percentage of carcass decreased significantly (P<0.01) due to consumption of 80 and 100% whey. Percentages of abdominal fat, liver, and pancrease were not significantly affected by the amount of whey. Percentage of ileum increased and showed significant (P<0.05) difference with control group. The regression equations for body weight and daily body weight gain, feed conversion and litter moisture in all ages, were significant (P<0.05) and polynomial. This indicated that consumption of whey by more than 40% caused reduction in broiler performance. No significant differences were observed in ileal contents of Lactobacillus and Entrobacteria due to different levels of whey, but total bacteria in ileum contents increased significantly (P<0.01) as whey content in drinking water increased.
F. Tajik,
Volume 8, Issue 1 (4-2004)
Abstract

Aggregation is an important temporal property of soil structure that is affected by intrinsic soil properties and also soil use and management. Aggregate stability has a strong influence on many processes in soil such as infiltration, aeration, strength, erosion, and soil’s ability to transmit liquids, solutes, gases, and heat. In this study, undisturbed soil specimens from 0-10 and 10-20 cm depths were sampled during summer 1999 from some regions in Iran including Golestan, Kermanshah, West Azerbaijan, and Mazendaran. After drying the samples in lab, the different sizes of aggregates were separated and the wet aggregate stability (WAS) and dispersible clay (DC) were determined on 2-2.8 mm aggregates according to Pojasok & Kay procedure (1990). The variance analysis of data showed significant differences among soils in all regions. The averages were compared by Duncan test to find the following order: Mazendaran > Golestan > Kermanshah > West Azerbaijan. Regression analysis of data of whole regions showed that the variability of aggregate stability was mainly explained by organic carbon content (R2=0.723 in P > 0.0001). The clay content had the greatest effect on aggregate stability in samples from Golestan while sand content had the greatest effect in samples from West Azerbaijan. The resulting equations from stepwise regression can be used to estimate aggregate stability from other soil variables in the study regions.
H. Khademi, H. Khayyer,
Volume 8, Issue 2 (7-2004)
Abstract

Understanding the variability of pedological properties as well as the soil quality attributes on different landscape positions in hummocky terrains would result in a better land management in such areas. Despite the importance of such studies, no research has been couducted on the landscape-scale variability of soil quality indices in Iran and most researchers have so far focussed on pedological aspects of soil variability. The objective of this study was to understand the variability of selected soil quality indices at different landscape positions. A systematic grid including 120 points (12x10) with a distance of 30m was laid out in a hummocky rangeland around the city of Semirom. Surface soil samples were taken from 120 points on grid nodes and their organic carbon, microbial respiration rate, phosphatase activity, pH and EC were measured. Also, the thickness of A horizon and the soil moisture content were measured on grid nodes in the field. The results indicated that the lower slope positions including footslope and toeslope had the highest amount of phosphatase activity, microbial respiration, A horizon thickness, organic carbon, and EC. In contrast, soils occuring on shoulder had the least amount of the above-mentioned properties. Soils on summit and backslope seem to have been moderately degraded. Soil pH showed the opposite trend, as compared to other properties studied. The presence of a great variability in soil quality attributes at the landscape scale can be attributed to differenes in effective moisture that various landscape positions receive, as well as the differenes in soil erosion and deposition rates. This can also be an indication of a severe land degradion due to poor management practices. Since applying different management practices on different landscape positions are practically impossible, to be on the safe side, it is highly recommended to plan conservation practices based on soil quality of the most degraded landscape positions.
R. Hajeeboland, N. Asgharzadeh, Z. Mehrfar,
Volume 8, Issue 2 (7-2004)
Abstract

A great number of studies have been conducted on the role of Azotobacter - a free living N2-fixer - in agricultural soils, but our information is lacking on the importance of Azotobacter in pasture soils. This research was conducted to study the relationship between ecological and edaphical factors with the population of the bacteria in soil. Soil samples were taken from two sites in Azarbaidjan (Mishou-dagh and Khadjeh), having different ecology, soils and vegetation types. We investigated also the effect of inoculation of rhizosphere with soil-isolated A. chroococcum on the growth of wheat (Triticum aestivum L. cv. Omid) during 56 days of growth in a pot experiment. The treatments included: 1) inoculation with A. chroococcum (+A), 2) N fertilizer at a rate of 35 mg/Kg as (NH4)2SO4 (+N), 3) +A+N and 4) –A–N as control with four replications. Results showed that the population of Azotobacter chroococcum correlated positively with organic carbon and negatively with soil pH. In soils covered with vegetation the population of bacteria was higher than that in uncovered soils and the highest population of bacteria was found in soil samples collected from the rhizosphere of grass species and the lowest in that of legume species. In inoculation experiment, shoot and, particularly root growth, increased in the order of +N, +A and +A+N in comparison with control (–A–N) plants. These treatments also stimulate uptake and transport of K into shoot. Interestingly, the effect of +A treatment was significantly higher than that of +N and +A+N treatments. The same results were obtained for nitrogen. A higher uptake and particularly transport of K into shoot was attributed to low molecular weight organic molecules produced in inoculated roots and transport K into shoot. Results showed that inoculation with Azotobacter not only enhanced growth and chlorophyll content, but also stimulated uptake and translocation of ions into shoot. The stimulatory impact of inoculation is most likely a general effect that could also be important in micronutrients translocation into shoot.

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