JWSS - Journal of Water and Soil Science

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Phytoremediation is a new, in-situ and emerging remediation technology for contaminated soils. This technology, compared to other methods, is a sustainable, natural, relatively cheap and applicable to large scale area. Modeling phytoremediation provides quantitative insight for the governing process as well as for managers to assess the remediated sites. The objective of this study was to introduce a macroscopic phytoremediation model for Ni and Cd- polluted soils. The proposed model assumes that relative transpiration reduction function can resemble total soilNi and Cd concentrations. Combining the related functions of soil and plant responses to soil Ni and Cd concentrations, the phytoremediation rate of Ni and Cd was predicted. In order to test the proposed model, large quantities of soil were thoroughly polluted with Ni and Cd. Upland Cress (Lepidum sativum) and Ornamental Kale (Brassica olerace var. Viridis) seeds were then germinated in the contaminated soils. The experimental pots were irrigated with fresh water to reach field capacity. Upland Cress and Ornamental Kale were harvested three and four times, respectively. At each harvest, relative transpiration, Ni and Cd contents of soil samples and plants were measured. Comparison of the maximum error, root mean square error, coefficient of determination, modeling efficiency and coefficient of residual mass indicated that the non-threshold non-linear model provide high efficiency to predict relative transpiration for Upland Cress and Ornamental Kale, respectively. The results also indicated that the proposed macroscopic model can well predict the phytoemediation rate of the Ni and Cd by Upland Cress (R2>0.83) and Ni by Ornamental Kale (R2=0.78).

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Most part of the applied phosphorus is fixed in the calcareous soil. Some part of residual phosphorus can be recovered by the plants of the following years. It is reported that organic matter is one of most important factors in phsphorus recovering. This expriment was designed in permanent plot to evaluate the effect of organic matter on residual phosphorus recovering in a calcareous soil. The expriment was conducted on randomized complete block design in a factorial manner with three replications in four years. Treatments were consisted of farm yard manure(FYM) in three levels ( 0, 15, and 30 ton ha-1) and three levels of phosphrus ( 0, 90 and 180 kg ha-1 as P2O5 from triple super phosphate (TSP). Phosphorus fertilizer was applied only in the first year of expriment, but FYM was used in all of the years. Onion, canola, bean and spinage were planted in the four years of experiment, respectively. Results showed that maximum yield, P concentratin in plant and soil phosphorus availability were followed by application of FYM and P(residual) together in all of the years. This increase was mainly by direct effect of P in the first year of expriment, but recovered phosphrus by FYM had maximum role in the later years. More than 53% of increase in phosphrus availability in soil and 21% of increase in yield resulted from recovered phosphprus by FYM in the treatment of 30 ton.ha-1 FYM and 180 kg.ha-1 P2O5 together in the last year.

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The Clark method is one of the most applicable techniques for development of instantaneous unit hydrograph whose efficacy depends upon the accuracy in estimating storage coefficient. The present study was conducted in Kasilian watershed in Mazandaran Province to determine the efficiency of developed hydrograph using Clark's method and to compare the Muskingum storage coefficients obtained through graphical, Clark, Linsley, Mitchell, Johnstone-Cross and Eaton methods. To this aim, the time-area histogram of the study watershed was initially developed. The 3h-unit hydrograph was then derived using the data collected in Sangedeh climatological and Valikbon hydrometric stations. The efficiency of Clark’s instantaneous unit hydrograph developed based on 6 methods for calculation of Muskingum storage coefficient was ultimately compared with the observed average 3h-unit hydrograph of the study area. The results of the study revealed that the Clark’s instantaneous unit hydrograph obtained from graphical method for estimation of storage coefficient with estimation error of less than 33.33% and efficiency coefficient of 83% could suitably simulate different components of the observed average unit hydrograph for the study watershed.

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In this research, logistic regression analysis was used to create a landslide hazard map for Sajaroud basin. At first, an inventory map of 95 landslides was used to preduce a dependent variable, which takes a value of 0 for absence and 1 for presence of landslides. Ten factors affecting landslide occurence such as elevation , slope gradient, slope aspect, slope curvature, rainfall, distance from fault, distance from drainage, distance from road , land use and geology were taken as independent parameters. The effect of each parameter on landslide occurrence was determined from the corresponding coefficient that appears in the logistic regression function. The interpretation of the coefficients showed that road network plays the most important role in determining landslide occurrence. Elevation, curvature, rainfall and distance from fault were excluded from the final analysis because these variables did not significantly add to the predictive power of the logistic regression. After transferring final probability function into Arc/view 3.2 software, landslide susceptibility map was prepared. The results of accuracy assessment showed that overall accuracy of produced map is 85.3 percent. Therefore, 53% of the area was located in very low hazard, 18.3% in low hazard, 21% in moderate hazard and 7.7 % residual area is located in high hazard regions. Model and then susceptibility map verity was assessed using -2LL, Cox and Snell R2, Nagelkerk R2, and was validated.

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Presence of petroleum contaminants in soil may be toxic to human and organisms and act as a source of ground water contamination hence, remediation of these compounds from environment is vital. In this study, first the feasibility of remediation of two petroleum-contaminated soil samples around Tehran Oil Refinery (oil refinery landfill and agricultural soils) was assessed using landfarming technique during a four month experimental period. The elimination of total petroleum hydrocarbons (TPHs) from soils treated through landfarming technique was then investigated in the rhizosphere of agropyron and fescue. The results showed that microbial respiration increased due to landfarming processes in both soils. Urease activity in the landfarming treatment for agricultural soil was 21, 45, 26, and 23% higher than the control at the end of first to the 4th months of experiment, respectively. However, no significant differences were observed between the landfarming and control treatments for landfill soil at the end of experiment. Furthermore, about 50 and 57% reduction in TPH-concentration was observed in the landfarming treatment for landfill and agricultural soils at the end of experiment, respectively. In the phytoremediation study, presence of TPHs in both landfarming and control treatments reduced dry matter yield of the studied plants. Urease activity in the rhizosphere of fescue and agropyron was higher than in the unplanted soil. Degradation of petroleum-compounds in the landfill soil under landfarming treatment was more than 20 and 40% in the presence of fescue and agropyron, respectively. The influence of agropyron on TPH-removal from agricultural soil under the landfarming treatment was also higher than fescue.

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In this study, the effects of enhancing synthetic chelators (HEDTA, EGTA) and low molecular weight organic acids (LMWOA) such as citric acid were compared on cadmium (Cd) solution in soils that were artificially contaminated. Also Cd phytoextraction capability by radish (Raphanus Sativus L.) was studied. The experiment was laid out in a randomized complete factorial design where each treatment was replicated three times. Concentration treatments of cadmium using CdCl2 were 0(control), 5, 20, 60 and 100 mg Cd kg-1. After complete growth of plants, 6, 20 and 20 mMkg-1 soil HEDTA, EGTA and Citric Acid were added per pot, keeping a control without any chelator application. In order to determine cadmium concentration ten days after adding chelates, samples were taken from the plants and soil of pots. The results showed that in all treatments the concentration of soluble Cd in soil was higher than the control. Also the results showed that synthetic chelators as compared with LMWOA (Citric Acid) have increased the solution remarkably. Among the other chelates, HEDTA had significant effects on Cd solution. In the current study, Cd concentration in shoot and root of (Raphanus Sativus L.) was increased with enhancement of Cd concentration in soil. Cd concentrations in shoots of radish were higher than those in roots. This could refer to higher bioavailability and solubility of Cd. In the current study, in all the treatments with HEDTA Cd concentrations in shoot and root of (Raphanus Sativus L.) were increased as compared with other chelates..

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Many experiments have been carried out to decrease the negative effect of drought stress and obtain suitable growth under water deficit conditions. Application of plant growth regulators (especially growth retardants) is one of the proposed methods. This study was conducted to investigate the effect of Cycocel application on growth of two olive cultivars (Shengeh and Roghani) under water stress condition. The design of experiment was completely randomized with six replications (Factorial arrangement). Treatments included irrigation intervals (2, 4, 6, 8 weeks) and Cycocel concentrations (0, 500,1000,2000,4000 mg per litter). Some indices such as height, chlorophyll, leaf area, root, and shoot weight (fresh and dry) were measured. Results indicated that interaction of C.C.C treatment and irrigation intervals on height of olive cultivars was not significant, probably due to the response of these cultivars to concentrations of C.C.C. Although drought stress decreased the number of leaves in both cultivars, the application of CCC (500 mg per litter) thwarted the adverse effects of drought stress. Cycocel increased chlorophyll content significantly (500 mg per litter in Roghani and Shengeh and 6 weeks irrigation interval). Cycocel application decreased leaf area, but in Shengeh cultivar increased leaf area in 6 week irrigation interval. Overall, the results indicated that the application of Cycocel with concentration of 500 mg per litter in 6 week irrigation intervals in both cultivars (Roghani and Shengeh) was the best treatment for controlling the adverse effects of drought. Also, results indicated that such changes were dependent on Cycocel concentration and drought level.

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In this research, based on the observed data of Class A pan evaporation and application of non-linear regression (NLR), artificial neural network (ANN), neuro-fuzzy (NF) as well as Stephens-Stewart (SS) methods daily evaporation of Kerman region was evaluated. In the cases of NLR, ANN and NF methods, the input variables were air temperature (T), air pressure, relative humidity (RH), solar radiation (SR) and wind speed (U2) which were used in various combinations to estimate daily pan evaporation (Ep) defined as output variable. Performance of the methods was evaluated by comparing the observed and estimated data, using determination coefficient (R2), root mean square error (RMSE) and mean absolute error (MAE). Based on the observed data at Kerman meteorological station, the monthly and annual average evaporation values of the region were 272 and 3263 mm, respectively. The results of this study indicated that NF method is the most suitable method to estimate daily Class A pan evaporation. The statistics criteria of this model which is constituted based on the 5 input parameters were R2 = 0.85, RMSE=1.61 and MAE= 1.24 mm day-1. The sensitivity analysis of NF model revealed that the estimated EP is more sensitive to T and U2 (as the input variables), respectively. Due to weak accuracy of SS method, a new modification step of the model was also developed based on the SR and T in order to have a more exact daily evaporation estimation of the region. However, the result of the modified model was not acceptable

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Organic matters are the most important factors that affect soil compactability and physical characteristics. In order to study the effect of pistachio waste on physical characteristics of two soils, a factorial experiment was conducted in a completely randomized design with three replications in a greenhouse. The treatments included pistachio waste at 4 levels (0, 3, 6 and 9 w/w %) and two types of soil texture (silty clay loam and sand).The results showed that the bulk density of sandy soil was decreased at high levels of waste application before compaction but had no significant effect on the bulk density of clay soil. The penetration resistance of both soil types was decreased by pistachio waste application. Soil water holding capacity increased and moisture curves shifted up for higher levels of organic matter application, while compaction curve reciprocally shifted into the lower levels by incorporation of wastes into the soils. At higher levels of organic matters, maximum bulk density was decreased and critical moisture was increased specially in fine texture soil. After compaction, the application of pistachio waste significantly reduced penetration resistance in silty clay loam soil relative to control but in sandy soil its effect on penetration resistance was only significant at maximum level (9 %).

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In order to study the effects of different land uses on soil development, a loess hillslope was selected in Agh-Su area, eastern Golestan Province. Six profiles in four land uses including pasture, Quercuse natural forest, Cupressus artificial forest and a cultivated land, were dug and studied. Samples from different horizons were collected for physico-chemical and microscopic analyses. Important physical and chemical attributes such as bulk density (Bd), mean weight diameter (MWD), Organic carbon (SOC), cation exchange capacity (CEC), soil calcium carbonate (CCE) and available P were compared in land uses. Organic matter, CEC and MWD were significantly lower in the cultivated land use. Organic matter content in the forest and pasture area was considerably higher than that of cultivated land use. Soil profile development studies revealed that forest soils were highly developed. Quercus natural forest soils were classified as Calcic Argixerolls. Unlike cultivated soils which showed the minimum development and were classified as Typic Calcixerepts, formation of argillic horizon with dominant speckled b-fabric in the natural forest indicated the high landscape stability. Crystallitic b-fabric of horizons showed the absence of enough leaching of carbonate and the subsequent migration of clay particles. Intense erosion of the surface horizons of cultivated land use resulted in the outcropping of the subsurface carbonate rich horizon preventing soil development. The soils of pasture and Cuprecuse soils had mollic epipedon and were classified as Typic Calcixerolls with moderate development. Micromorphological properties of soils can help consider changes in pedogenic processes occurring under different land covers.

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Phosphorus is often considered the most limiting soil nutrient for plant productivity. Several extractants have been typically used to measure available P. Organic and inorganic P fractions are not measured by common soil test, and thus fractionation schemes using various extractants have been developed over years to quantify the different chemical forms of P in soils. The objectives of this research were: i) to investigate the effect inorganic P fractions on Phosphorus availability ( Olsen procedure), and ii) to evaluate the relationships between these P fractions and Garlic (Allium sativum) plant indices. Soil inorganic P fractions were determined in 30 surface soil samples (0-30 cm) from various regions of Hamedan Province. Inorganic P fractions included dicalcium phosphate (Ca2-P), octa calcium phosphate (Ca8-P), P adsorbed by Al oxide (Al-P), P adsorbed by Fe oxide (Fe-P), P incorporated into Fe oxide (O-P), and apatite (Ca10-P). Total P and Organic P were measured in these soils. In order to evaluate the availability of different forms of P to plant, a pot experiment was conducted on Garlic plant. The treatments including two rates of P (zero and 150 mg kg-1) and 9 soils from 30 soil samples were arranged in a factorial experiment in a completely randomized design with three replicates. The results showed the total P with an average of 1501 mg kg-1 soil, and organic P comprised 19 % of the total P. The inorganic P fractions (Ca2-P), (Ca8-P), (Al-P), (Fe-P), (O-P), (Ca10-P) and residual phosphate (Res-P) comprised 4.7, 20, 8.7, 3.0, 2.8, 24 and 18 % of total P, respectively. Most of the inorganic P occurred as Ca-P and comprised 49% of total P. The correlation study between different P fractions showed that Ca2-P, Ca8-P, Ca10-P, and Al-P were significantly correlated with Olsen-P. This suggested that probably Olsen procedure essentially removed some P from these fractions. The results of pot experiment indicated that plant indices (relative yield and Yield efficiency) were significantly correlated with Ca2-P, Ca8-P and Olsen-P (P ≤ 0.05). P availability in the studied soils was influenced by Ca2-P and Ca8-P which could be used in a long period of time by plant.

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A greenhouse experiment was conducted in factorial experiment arranged as a completely randomized design (CRD) to evaluate the role of tree indigenous arbuscular mycorrhizal fungi (AMF) species originated from a polluted soil in phytoremediation of zinc polluted soils using maize as a host plant. The experiment consisted of plants inoculated with AMF (G1(Glomus intraradices), G2(Glomus mosseae) and G3(Glomus versiforme)) and G0 as non-inoculated plants and 5 levels of zinc (0, 10, 50, 100 and 500 mg kg -1) in non-sterilized sandy loam soil with three replications. According to the results of greenhouse experiment, the zinc and phosphorus uptake and also the biological yield of maize plants were significantly increased by inoculation with AMF in comparison with non-inoculated plants and also no zinc toxicity symptoms were observed. Uptake, translocation, and phytoextraction efficiency of plants inoculated with G. intraradices was more than the other treatments up to the level of 100 mg kg -1, but at the level of 50 mg kg -1 these amounts were highest in plants inoculated with G. mosseae. The efficicncy of three AMF in zinc uptake was highest at the low level of zinc. In general, under the high soil pollution (500 mg kg-1), G. mosseae was the most effective fungal species in Zn extraction and translocation while G. intraradices had the highest effectiveness for accumulation of Zn in the roots. The overall situation of G. versiforme was mostly between the two other fungal species.

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The positive and beneficial effects of mycorrhizal symbiosis on the growth of various plants have already been documented. Most arable crops can mutually establish symbiosis with endo-mycorrhizal fungi, however, mycorrhizal associations with almond (prunus amygdalus), particularly in calcareous soils of arid and semi-arid regions, have not been studied. Thus, to realize the symbiotic association between mycorrhiza fungi and economically important native almond genotypes in Chaharmahal va Bakhtiary province, an experiment consisting of a randomized complete factorial design with three factors: almond genotypes (Mamaei, Rabee, Talkh, Sefid), soil phosphorus levels (0 and 150 kg P ha-1) and mycorrhizal treatments (almond inoculated with Glomus intraradices, Glomus mosseae and without inoculation) with three replications was conducted under greenhouse conditions by 4 months. Results showed that physiological traits including chlorophyll concentration, net photosynthetic rate and water use efficiency, were significantly increased for 20%, 300% and 300%, respectively in mycorrhizal almond plants, while transpiration rate was decreased (8-10%). Although the genotype of almond and soil P were dependent on these factors, mycorrhiza species had the same effects. Increased available P in the soil enhanced the growth in all treatments and photosynthesis of nonmycorrhizal almonds.

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Many studies have been carried out on the effect of organic matter on soil physical, chemical, biological, and nutritional properties, including the effect of organic matter on the availability of such elements as P, N and heavy metals. There is, however, no information on the effect of organic matter on potassium uptake from micaceous minerals. The objective of this study was to investigate the effect of organic matter on potassium uptake from micaceous minerals released by alfalfa. An experiment was laid out in a completely randomized design with factorial combination and three replicates. Growth medium was a mixture of quartz sand, micaceous mineral (muscovite or phlogopite) and organic matter (0, 0.5 and 1 %). Rehnani cultivar of alfalfa was used in the experiment. During 120 days of cultivation, plants were irrigated with either complete or K-free nutrient solution and distilled water as needed. At the end of cultivation, plant shoots and roots were separately harvested and their K concentration was measured by flame photometer following dry ash extraction. Under the K-free nutrient solution, a significant increase in biomass occurred in pots containing phlogopite and organic matter as compared to those with no organic matter amendment. Also, under K-free condition, potassium concentration in shoot was above the threshold value only in phlogopite amended pots. There was no significant difference in K concentration among different levels of organic matter in control treatment as well as in muscovite added treatment. Under both nutrient solutions treatments, significant increase of K uptake occurred in pots containing phlogopite and organic matter, as compared to those without it. In contrast, under K free nutrient solution, organic matter amendment could not enhance the K uptake in pots containing dioctahedral mica (muscovite). Root activities and organic matter decomposition appear to have increased rhizosphere acidity which, in turn, facilitate the K release from trioctahedral mica (phlogopite) in K deficient medium. Thus, the effect of organic matter on K release greatly depends on the type of micaceous mineral.

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The petroleum refinery sludge is an important source of environmental pollution. Burning and burying of the sludge may have adverse effects on environment and human health. Thus, other mechanisms for decreasing the toxic effects of hydrocarbon substances in the sludge must be used. In this study, Isfahan refinery sludge was dewatered, air dried and mixed by 0, 10, 20, 30 and 40% w/w ratio with two calcareous soils, viz., Mahmoud Abad (Typic Haplocalcids with clay texture) and Bagh Parandegan (Anthropic Torrifluvents with silty loam texture). Different mixtures of soil and sludge were farmed for 21 days and irrigated on a daily basis to field capacity. Then, 100 seeds of Tallfescue (Festuca arundinacea) and Agropyron were planted in polluted soils with 3 replicates in 3 kg pots for 5 months. Result showed that Tallfescue and Agropyron yields decreased in sludge contaminated treatments. In the 40% sludge treatment, Tallfescue decreased the total petroleum hydrocarbons content by 65 percent. The highest degradation for agropyron was in the 30% sludge treatment which showed about 55% reduction in total petroleum hydrocarbons. The 40% sludge treatment resulted in the minimum yields of root and shoot plants. The highest degradation of TPHs occurred in the Tallfescue rhizospher of 40% sludge. Maximum degradation of TPHs on the Agropyron rhizospher was in 30% sludge mixed with Bage parandegan soil, but maximum yield of plant was in 20% sludge. Our study shows that Tallfescue rhizospher is most effective for decreasing TPHs, and that the phytoremediation in soils with more clay can adsorb and fix the toxic components and then at higher levels of pollutions can let the plants grow.

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Biological soil covers such as lichens have critical roles in soil stability and prevention of erosion. In order to study the effect of lichen biological covers on aggregate stability and soil conservation, loess hills covered with lichen and uncovered ones were selected in Northern Golestan Province. Five samples were taken from the depth 0 to 5 cm of both two areas for physico-chemical analyses. The undisturbed soil samples were taken for micromorphological studies, too. Analyses of soils revealed that soil organic carbon content increased markedly, compared to uncovered soils. Mean weight diameter also increased about three folds in soils covered with lichen. Fungal hyphae and polysaccharides excretions bind soil particles together and increase size of aggregates. Micromorphological study of thin sections showed that uncovered soils had a weak and massive structure, but soils covered with lichen had a crumb granular and also well -separated angular block and higher proportion of voids. Due to the effect of lichen on upward movement of calcium carbonate, crystallitic b-fabric appeared in the surface layer of covered soils and speckled b-fabric underneath. Excremental pedofeatures are the most common pedofeatures in the covered soils.

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Performance of proper deep tillage can reduce negative effects of soil compaction. The objectives of the research were to determine the effects of deep tillage application on soil physical properties and wheat yield. Therefore, in 2006-2007 an experiment was conducted at Tajarak Agricultural Research Station of Hamedan on a soil with clay loam texture. In this research, four tillage methods were used. These methods were: 1) subsoiling with 30cm soil depth + conventional tillage (moldboard plowing) 2) subsoiling with 50cm soil depth + conventional tillage, 3) plowing by combined plow (chisel plow + roller) with 30cm soil depth and 4) conventional tillage (plowing by moldboard plow to 25-30cm soil depth). Randomized complete block design with four treatments and replications was used to analyze data and compare the treatment means. Before and after the application of the tillage treatments, parameters of cone index, bulk density and infiltration rate were measured in soil. Also before yield harvesting, components of yield (grain number in panicle, panicle number in plant and 1000 grain weight) were measured. To measure grain yield in experimental plots, in each plot ten square meter area from the yield was harvested. Result indicated that effect of different tillage treatment on soil resistance was not significant. Effect of deep tillage methods on bulk density and infiltration rate was significant. Deep tillage methods increased water infiltration rate in the soil and decreased soil bulk density. Effect of different deep tillage methods on wheat yield was not significant. However, application of chisel plow increased the wheat yield by 25%. It may be concluded the subsoiling treatment does not affect the crop yield in intensive and fully irrigated field condition. The results of this study indicated that if there is a compacted layer at 30cm soil depth, and with the same climate and soil as those in the experimental site, the application of chisel plow due to economical consideration (low fuel consumption and high field efficiency) can be recommended for soil preparation and cultivation of irrigated wheat.

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Effect of drought stress and mycorrhizal symbiosis on growth and phosphorus (P) uptake by two sorghum cultivars different in root morphology was studied in a pot experiment. A factorial experiment with a randomized complete block design was performed. In this study, sorghum speed feed cultivar with high root proliferation and branching and sorghum KFS2 cultivar with low root proliferation and branching were colonized by Glomus intraradices. All plants were watered equally in the first three weeks to establish them. Drought stress was applied from the week four and the plants were watered when 40% (T1), 60% (T2) and 80% (T3) of the available water was consumed. Results showed that in both sorghum cultivars, plant dry weight decreased as drought stress was increased. However, dry weight of mycorrhizal plants was higher than that of non-mycorrhizal plants at all levels of drought stress. This increase for speed feed cultivar was greater than for KFS2 cultivar. Phosphorus concentration and total P uptake in shoot of both mycorrhizal plants were greater than those of non-mycorrhizal plants. P accumulation in both cultivars decreased by increasing drought stress due to the decline in plant biomass. However, P accumulation per unit length of colonized root increased by increasing drought stress. The greater percentage of root colonization and the increased total length of external hyphae per unit length of colonized root of KFS2 cultivar led to an increase in mycorrhizal growth response and improved P nutrition of KFS2 cultivar compared to those of speed feed cultivar.

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The growth and fluorescence parameters of chlorophyll in four corn hybrids including two sweet (K.S.C. 403 and K.S.C. 404) and two grain hybrids (S.C. 500 and S.C. 700) were evaluated in response to Fe and Zn nutrition in a nutrient solution culture. This study was conducted in a randomized complete block design with a factorial arrangement with three replications at the Soilless Culture Research Center (SCRC). Corn hybrids were exposed to two Fe levels (5 and 50 µM Fe in the form of FeEDTA) and two Zn levels (0 and 2 µM Zn in the form of ZnSO4). Increasing Fe concentration in the nutrient solution increased F0, Fm, and Fv/Fm values by 48, 96, and 123%, respectively. The fluorescence parameters of chlorophyll were affected by corn hybrid and there were significant differences in F0 and Fv/Fm at 1% level and Fm at 5% probability level among the studied hybrids. Iron deficiency significantly (P<0.05) reduced the root dry weight of all the studied hybrids. Zinc deficiency resulted in a significant decrease in the root dry weight of H403 and H500 hybrids while no significant decrease was found in the root dry weight of the H404 and H700 hybrids under Zn condition. The results showed that corn hybrids varied significantly in their tolerance to Fe and Zn deficiency. Based on the results of plant growth and fluorescence parameters of chlorophyll, the studied grain corn hybrids were more tolerant to Fe and Zn deficiency conditions in comparison with sweet corn hybrids. It seems fluorescence parameters of chlorophyll might be suitable parameters to screen corn hybrids in their tolerance to Zn and particularly Fe deficiency condition.

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Baseflow separation has long been an important topic in hydrology and has a crucial role in water resources management in arid and semi arid regions like Iran. In this paper, a comparison among commonly used automated techniques for hydrograph separation including theoretical method of local minimum and digital filter of one parameter with different filtering parameters of 0.9 to 0.975 and two parameter methods was done to estimate baseflow using baseflow index. For this purpose, daily flow data in some stream gauging stations in west Azarbaijan province were used. For comparison, in addition to baseflow index the graphical method based on the observed daily flow data and correlation coefficient among them was utilized. The main aim of this research is distinguishing the most suitable method in hydrograph separation and estimating the baseflow. Results showed that in different methods baseflow largely contributes to streamflow and also has high fluctuations. However, the results of the digital filter with two parameters appear to be hydrologically more plausible than those of the other methods, but the results of digital filter with proper parameter - in this region one parameter method with filter of 0.925- has proper estimation accuracy. Also, the baseflow index based on method of two parameter digital filtering varies from 0.54 to 0.78 in this study area.