JWSS - Journal of Water and Soil Science

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Floodwater Spreading (FS) plays an effective role in improving soil fertility, ground water recharge, vegetation cover, and desertification control. The soil fertility might increase as a result of a suitable suspended sediment material transferred to the downstream by flood events. To define a relevant FS method which increases the efficiency of the FS projects, it is necessary to study the quality and quantity of transported sediment material, spatially and temporarily. In this research, this subject was investigated by taking soil samples throughout 13 FS stations for physical and chemical analysis over 5 years. Within each of the 13 selected stations in the three first flooded dikes, soil sampling was carried out using random-systematic method. The total Nitrogen, absorbed Phosphorous and Potassium, and Organic Carbon of each sample were analyzed. Because of the abnormality of data, nonparametric test was adopted to compare means. All stations were classified into three groups using cluster analysis method. Based on the results, the variations of fertility factors are irregular between the dikes and amongst years. This could have been affected by several factors such as the quality and quantity of diverted flood, the characteristic of FS sites, and irregularity of sediment material deposited on the sites. Despite the low quality of soil fertility prior to the construction of these stations, in general, FS has a considerable role in improving the soil fertility. However, desirable objectives may be achieved in long term through occurrence of diverse flood events and suitable maintenance of the stations.

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Azotobacter chroococcum can improve mineral nutrition of plants through N2 fixation and plant growth promoting capabilities. Fourteen strains of A. chroococcum were isolated from rhizosphere of wheat plants grown in different field conditions around Tabriz, northwest of Iran. In a pot culture experiment with sterile soil, wheat plants (Triticum aestivum cv. Falat) were inoculated with 14 bacterial strains. Positive control received nitrogen fertilizer without bacterial inoculation and the negative control was left un-inoculated and without N- fertilizer. Totally, 16 trearments with four replications were arranged in a completely randomized design. The plant growth indices and N and P concentrations of shoot and root were determined at the harvest time. Results showed that the inoculation with Azotobacter strains caused a significant increase in shoot and root dry weights. Bacterial inoculation significantly enhanced the concentration and content of N in shoot and root. Phosphorus content was only enhanced (p<0.05) in the root. Translocations of N and P from root to shoot were markedly increased in bacterial treatments compared to the positive and negative controls. Moreover, strains 1 and 48 which showed relatively higher phosphate solubilizing capability and phosphatase activity in in-vitro assay also brought about higher P content and concentration in shoot and its translocation from root to shoot.

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Estimation of flood hydrograph is of necessities in hydrological studies such as flood mitigation projects. This estimation in un-gauged watersheds is usually taken place using geomorphological characteristics of watersheds. The objective of this research is to estimate synthetic unit hydrograph using regional flood frequency analysis and geomorphological parameters of watersheds. 1-hour and 2-hour hydrographs of two watersheds, Kanisavaran and Maranj Watersheds, were generated using maximum discharge data based on regional flood frequency analysis. Estimated hydrographs were compared with observed data and the efficiency of the model was evaluated using Nash-Sutcliffe coefficient, absolute and bias errors. The results showed that multiple regression models give more acceptable results among others for the computation of synthetic unit hydrograph (higher coefficient of determination). The Nash-Sutcliffe coefficient was 0.98 for 1-hour hydrograph while it was 0.93 for the 2-hour hydrograph. The absolute error in 1-hour hydrograph and 2-hour hydrograph was 0.13 and 1.2, respectively. The bias error was close to zero for both hydrographs, indicating that the proposed model is efficient. The model may be used for estimation of synthetic unit hydrograph in similar un-gauged watersheds.

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In order to investigate the effect of vermicompost on physical and chemical properties of soil, an experiment was carried out in split plot based on complete randomized block design in three replications in Sari Agricultural Sciences and Natural Resources University. The physical and chemical properties of soil included bulk density, particle density, total porosity, water holding capacity, field capacity, permanent wilting point, available water capacity, pH, organic carbon and electrical conductivity in soil. Six levels of fertilizer treatments (T1= control, T2= chemical fertilizer, T3= 20 tons vermicompost + 1/2 T2, T4 = 20 tons / hac vermicompost + 1/2 T2 , T5= 40 tons vermicompost + 1/2 T2 and T6= 40 tons / hac vermicompost) and three levels of application years, one year of fertilization (1385), two consecutive years of fertilization (1385 and 1386) and three consecutive years of fertilization (1385, 1386, 1387). The results of the study showed that the application of these treatments in soil were significantly effective in increasing the total porosity, water holding capacity, field capacity, permanent wilting point, available water capacity, organic carbon electrical conductivity and in decreasing the bulk density, particle density and pH compared to control. In Contrast years of consumption of fertilizer did not have any significant effect on the physical properties of the soil except for FC, PWP, AWC, pH, OC and EC. The interaction between years of consumption of fertilizers were significantly different only in particle density and field capacity.

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Arbuscular mycorrhizae (AM) and Plant Growth Promoting Rhizobacteria (PGPR) associations are integral and functioning parts of plant roots. These associations have a basic role in root uptake efficiency as well as improvement of plant growth in degraded environments including heavy metals contaminated soils. This study was conducted to evaluate the effects of heavy metal-resistant soil microbe's inoculation on bio-availability of Pb and Cd in soil, plant growth as well as metal uptake by Millet (Pennisetum glaucum), Couch grass (Triticum repens) and wild alfalfa (Medicago sativa). A soil sample was treated by different levels of Pb and Cd (soil 1). Native microbial inoculums were obtained from alfalfa rhizosphere soils adjacent to Pb and Cd mines in Zanjan region (soil 2), then added with weight ratio of 1:5 (w/w) to soil 1. Host plants including millet, couch grass, and alfalfa were grown in pots and kept in greenhouse conditions. At the end of growing period, shoot dry matter and Pb and Cd concentrations in plant and soil were measured. Results indicated that plants yield and Pb uptake were significantly higher in non-inoculated treatments (p ≤ 0.05). However, Cd uptake by plants was greater in inoculated treatments (p ≤ 0.05). Couch grass showed the most accumulation potential of Cd and Pb among the studied plants.

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Paleosols provide invaluable data on paleoclimatic conditions of the area. These soils widely exist in central Iran. Micromorphology and clay mineralogy are among valuable techniques which are useful for interpretation and identification of these soils. The present research was performed to compare the micromorphology and clay mineralogy of paleosols and modern soils of Jiroft area. After field studies, 4 pedons (located on different geomorphic surfaces including stable mantled pediment, stable and unstable transitional surfaces of pediment and alluvial plain, and stable surface of alluvial plain) were sampled for physicochemical, micromorphological, and clay mineralogical analyses. Clay coatings in argillic horizons of paleosols were found during micromorphology observations. On the other hand, clay coatings in present soils were only found in natric horizons, which were attributed to high amounts of Na in these soils. Moreover, smectite, palygorskite, illite, chlorite, and kaolinite clay minerals were recognized in paleosols, but chlorite was not detected in modern soils. The presence of palygorskite in the soils under study was related to the stability of geomorphic surface. Results of the present research showed that a more humid climate was present at the time of paleosols formation.

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Contamination of agricultural soils by heavy metals is a serious threat from both agricultural and environmental standpoints. Among heavy metals, cadmium (Cd) toxicity for humans and plants is of great concern due to its high mobility and phytoavailability in soil even at low concentrations. Opposite to Cd, Phosphorus (P) is an essential nutrient for plant growth. A greenhouse experiment was carried out in a completely randomized design to examine the influence of Cd, P and their interaction on the growth and chemical composition of spinach grown on a calcareous soil. Treatments consisted of four Cd levels (5, 10, 20 and 40 mg/kg soil as cadmium sulfate) and four P levels (0, 20, 40 and 80 mg/kg soil as mono-calcium phosphate) in three replicates. The results indicated that 40 mg Cd significantly decreased spinach dry weight by 47% but P application decreased detrimental effect of Cd on spinach dry weight. Also, phosphorus application significantly decreased Cd concentration in spinach aerial parts by 78%. Increasing Cd levels increased concentration of this element in spinach shoots. Addition of 40 mg Cd decreased P concentration by 21.5% in aerial parts. Zinc (Zn), manganese (Mn), calcium (Ca), and nitrogen (N) concentration significantly decreased with P application but increased concentration of sodium (Na). Cadmium application decreased Zn concentration but increased Ca, Na and N concentrations in spinach. Our tentative conclusion is that P application in P-deficient soils is probably effective in decreasing Cd concentration and the detrimental effect of Cd on spinach growth, indicating a negative interaction between these elements. Prior to any fertilizer recommendation, the results of this experiment should be verified under field conditions

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Waste tire rubbers are considered one of the environment pollutants. Increased production of these pollutants has led to more serious consideration of ways to reduce the harms caused by their accumulation in the environment. Therefore, the effects of incorporation of waste rubber crushed particles in two sizes of 1-2 and 3-5 mm and the amounts of 0, 5, 10 and 20 Mg ha-1 in a calcareous soil (0-30 cm depth) on some chemical properties of soil was investigated in Isfahan University of Technology research field (Lavark). This experiment was performed using seven treatments along with a non amended control treatment in a randomized complete block design with three replications in 1387. Eight months after incorporation, soil samples were collected for laboratory analyses. Results of ANOVA tables show that soil pH, electrical conductivity, percentage of total nitrogen and DTPA-extractable concentrations of cadmium, lead, copper and iron were not significantly affected by application of rubber particles. Incorporation of rubber particles into the soil significantly increased soil organic carbon and carbon to nitrogen. Increases in the DTPA-extractable Zn in soils treated with 10 and 20 Mg ha rubber particles in fine and coarse sizes were significant in comparison with the control soil. DTPA-extractable Zn content in the soil treated with 10 and 20 Mg ha 3-5 mm waste tire rubbers particles was about two and three times higher than that in the control treatment, respectively. The results of this study showed that in short-time, incorporation of crushed tire rubbers particles had no significant effect on most chemical properties of the soil but increased the available Zn content. In this regard, further studies to monitor the effects of adding waste rubber crushed particles on organic matter mineralization, plant toxicity and physical properties of soil in long-term are recommended.

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The application of herbicides as organic chemical compounds to control pest and weeds may affect the population and activity of microorganisms, and this may have an influence on biochemical processes that are important for soil fertility and plant growth. The primary objective of this study was to evaluate different loading rates of eradican (EPTC) on soil microbial biomass C and N, microbial biomass C/N ratio and the activities of urease and arylsulphatase under field conditions. In this experiment, loading rates of 6 and 9 L ha-1 eradican were applied to a calcareous soil cultivated with corn (Zea mays L.) and left uncultivated using split-plots arranged in a completely randomized block design with three replications. The experiment was conducted in the Kabootarabad’s Agricultural Research Center, Isfahan. Soil microbial biomass C and N were determined at 30th and 90th days after the onset of experiment and the activities of urease and arylsulphatase were assayed at 30th, 60th and 90th days. Results showed that in soils cultivated with corn microbial biomass C increased with increasing eradican levels and in both cultivated and uncultivated soils microbial biomass N and microbial biomass C/N ratios were increased over the control. At 30th day, urease activity at 6 L ha-1 level reduced, while at 9 L ha-1 level it increased compared with the control soils. At 60 day, there was no significant difference in the urease activity between the treatments. At 90th day, the activity of urease showed slight fluctuations. There was a reduction in arylsulphatase activity of the cultivated soils by increasing the loading rates of eradican during the experiment, and in uncultivated soils no trend was observed. Briefly, the use of eradican can cause either reduced or increased microbial biomass sizes and enzyme activities in calcareous soils These changes, however, depend largely upon the application rate of eradican, time elapsed since eradican application (i.e., sampling date) and the presence or absence of plant

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To evaluate the effect of nitrogen slowly released from pellet, composed of manure and urea fertilizer on the Nitrogen efficiency and morphological Characteristics and grain yield of corn hybrid (S.C704), a field experiment was carried out in Aboureihan research farm of Tehran University in 2009. The factorial design of the study comprised a randomized complete block with three replications. The application rates of N at four levels (46, 92, 138 and 184 kg N. ha-1) and two levels by methods of N distribution (pellet and mixed with soil) were applied. In this research, a Screw Extruder setup was designed and manufactured. Statistical analysis indicated that NUE, as well as agronomic efficiency (AE) was reduced while physiological efficiency (PE) increased with increasing N rates. Also, most plant length and stem diagonal and cob diagonal pellet belonged to the treatment. But, the number of leaves per plant did not affect the distribution method of fertilizer.The results showed significant differences among various rates of nitrogen and methods of N distribution considering grain yield and grain protein. The higher rates of N increased grain protein, grain yield and yield components (except for number of rows per ear). Maximum grain yield (11.1 t. ha-1) was obtained with 184 kg N. Ha-1 treatment.

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Vegetation is the most important factor in sustainable and dynamic equilibrium of natural ecosystems. Considering the relation between environmental factors and vegetation is an essential step in order to identify the effective factors in the habitats. The purpose of this study was to evaluate the relations between vegetation and soil characteristics in rangelands of Ferydounshahr (Sardab-Sibak watershed). The flora and vegetation types were studied using Physionomic-floristic method in the field and eight vegetation types were identified. Vegetation was sampled by Systematic–randomized method in 1× 2 m plots. The canopy cover and species composition percentage were estimated in each plot. After digging a soil profile in vegetation types, the physical and chemical factors were measured. The influences of edaphic factors on vegetation were analyzed using RDA ordination technique. Results showed that based on soil characteristics the eight vegetation types can be classified in to three main range habitats including, Ferula ovina, Astragalus adscendens and Astragalus brachycalyx. The most important factors which cause the separation in these three habitat ranges were clay percentage, organic matter, soil depth, CaCO3, pavement and barren soil.

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Soil contamination with heavy metals, including Cd and Pb, is of serious concern. The aim of this study was to investigate the efficiency of Atriplex verucifera, Salicornia europaea and Chenopodium album for simultaneous remediation of soil exchangeable sodium percentage and Cd and Pb contamination in two soils with different properties. Two soils, including a saline-sodic-calcareous (S1) and calcareous (S2) soil, were selected. Different concentrations of Pb and Cd were then added to the soils. The contaminated soils were incubated under a wetting-drying cycle for nearly seven months. The plants, seeds were grown in pots containing different treatments of polluted soils and in control treatment (no Cd and Pb contaminations). The plant yields and concentrations of Pb, Cd and Na in the soil and plant samples were measured. A considerable accumulation of soil Cd by Salicornia and Pb accumulation by Atriplex and Salicornia was observed under unsuitable conditions of the saline-sodic soil, whereas Atriplex and Chenopodium had high capability for Cd in the soil S2. Also these plants caused the reduction of ESP in soil S1. The results revealed that these plants could be used for remediation of Pb and Cd contaminated soils. In this study, Salicornia with lower rate of yield reduction had the highest tolerance to Cd-stress. Understanding the complex plant and soil (salinity-sodicity and soil metal concentration) factors controlling the metals concentrations in the plants will help to design phytoextraction technology for arid, salt-affected regions.

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Studies on the soils of western Golestan province show that regardless of increased rain and presence of clay minerals with high cation exchange capacity, potassium extractable with ammonium acetate is low. In order to find the reason for this low amount of available K, clay minerals and micromorphology of the soil porosity were studied. Twenty disturbed and undisturbed samples from each horizon were taken for physicochemical properties, mineralogy and micromorphological studies. Four selected profiles included Gypsic Aquisalids, Typic Endoaquepts, Typic Calcixerolls and Typic Hapludalfs. The results showed that in addition to the clay content and type of clay minerals in soils that can affect soil available K (Kava.), it seems soil porosity can also affect Kava. mainly through their effects on extension of roots, water and nutrients transmission. Favorable content of clay and dominance of smectites in Mollisols and also higher porosity and dominance of channel porosities caused the presence of higher Kava, in these soils. presence of HIS. poor soil drainage, reduction of Fe3+ in smectite crystal lattice in Inceptisols, and also less amount of porosities caused the presence of higher potassium fixation and reduction of Kava. in these soils.

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In order to study land degradation from the soil genesis and micromorphological perspective, ten soil profiles were dug and described on five slope positions in both forest and deforested cultivated land. The soil samples were taken from all horizons for physico-chemical analysis and micromorphological studies. Forest soils had a well developed argillic and calcic horizons and also mollic epipedon. These soils were classified as Alfisols and Mollisols. Carbonate leaching into the depth of soil profile and translocation of clays to lower layers and formation of developed soils are by no means related closely with the dense forest cover and its subsequent landscape stability and favorable leaching conditions. Dominant soil orders in this area were Inceptisols and Mollisols. In cultivated landuse, no argillic horizon was observed except in toeslope position. Absence of argillic horizon or its elimination following deforestation is one of the most important and obvious pedogenetic evidences of land degradation after land use change. Outcropping of high carbonatic layers (calcic horizon), disappearance of mollic and formation of ochric epipedon, presence of redoximorphic features attributed to runoff in lowland, decrease of solum thickness, and change of soil color were the other pedogenic indicators for land degradation in the study area. Microscopic observations showed that granular and crumb microstructures with high porosity were converted to massive and compact ones with low porosity in the deforested area. Disappearance of clay coatings besides absence of excremental pedofeatures were the other important micromorphological evidences of erosion and land degradation.

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Hardsetting phenomenon is an indicator of poor soil physical quality. Hardsetting soils are soils with high rate of mechanical strength increase upon drying and are hardened and/or compacted when dry out. It is difficult to till such soils. Hardsetting soils have additional limitations such as poor aeration at wet conditions, low infiltrability and high runoff and erosion. Most of Iran soils have low organic matter content and it is expected that hardsetting phenomenon occurs in some of these soils. This study was conducted to investigate the hardsetting phenomenon on 9 soil series collected from Hamadan province. Three types of mechanical strength consisting tensile strength (ITS), unconfined compressive strength (UCS), and penetration resistance (PR) were measured on the repacked soil samples prepared in the lab. The ITS, UCS and PR tests were done on the soil cores which had been prepared at bulk density (BD) equal to 90% of critical BD for root growth (0.9BDcritical). The effects of intrinsic properties on the hardsetting phenomenon were studied, too. Based on the suggested definition in “International Symposium on Sealing, Crusting and Hardsetting Soils” to International :::union::: of Soil Science, in which a hardsetting soil has air-dry tensile strength ≥ 90 kPa, one soil (medium-textured) out of the studied soils showed the hardsetting phenomenon at 0.9BDcritical. It might be concluded that medium-textured soils are more susceptible to hardsetting. For all of the studied soils, the ITS increased with the increase in clay content. The increasing impacts of clay and carbonate contents were also observed for the UCS and PR, respectively. Calcium carbonate could act as a cementing agent in between the soil particles and brings about the soil susceptibility to hardsetting. Moreover, the decreasing trend of all soil mechanical strengths was observed with water content increase. Slope (b) of the exponential model (fitted to the soil mechanical strength characteristic curve), as an index of hardsetting, had positive correlation with the sand content and negative correlation with the silt content. Overall, texture and calcium carbonate content are major and effective properties in terms of hardsetting phenomenon in Hamadan soils.

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Southern Oscillation Index (SOI) and Sea Surface Temperature (SST) patterns affect rainfall in many parts of the world. This study aimed to investigate the relationship between monthly and seasonal rainfall of Iran versus SOI and Pacific and Indian sea surface temperature. Monthly rainfall data, from 50 synoptic stations with at least 30 years of records up to the end of 2007, were used. Monthly and seasonal time series of each station were divided to several groups by four methods (Average SOI, SOI Phases, Indian SST Phases and Pacific SST Phases) using Rainman software and with regard to 0-3 months lead-time. Significant differences among rainfall groups in each method were assessed by the non-parametric Kruskal-Wallis and Kolmogorov-Smirnov tests, and the significant relationship was validated using Linear Error in Probability Space (LEPS) test. The results showed that SOI during summer (July-September) was related to autumn (October-December) and October rainfall in the west and northwest of Iran and the west Caspian Sea coast. The El Niño (negative) phase was associated with an increase in rainfall and the La Niña (positive) phase was associated with a decrease in rainfall in these regions. Average SOI is a useful index for rainfall forecasting in the above-mentioned areas. However, Indian and Pacific SST phases are not suggested for rainfall forecasting in Iran, duo to weak or non-persistence relationships. In conclusion, Iran rainfall is not limited to SOI, Pacific and Indian SST therefore, Rainman could not be used as an aid to water resources management over a year in Iran. It is suggested that we study the teleconnection between Iran rainfall and other ocean-atmospheric oscillations developing a model similar to Rainman in order to that we investigating the variation in Iran rainfall with aid of other effective ocean-atmospheric indicators

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Due to soil and plant zinc (Zn) deficiency and its effect on reducing yield and quality of agricultural products, application of Zn fertilizers has been intensified in recent years. To achieve optimum fertilizer management, knowledge of factors affecting Zn availability in soil and its uptake by plant is required. Therefore, this study was carried out to investigate the effect of certain soil physiochemical properties on Zn availability in soil and its uptake by corn. The experiment was laid out in a completely randomized design with factorial combination and three replicates in the research greenhouse of Isfahan University of Technology, in winter 2009. In this greenhouse experiment, 11 soil series were exposed to two Zn levels (0 and 15 mg Zn kg−1 in the form of zinc sulfate). The results indicated that Zn application significantly increased the dry matter weight and shoot and root Zn concentration of corn, although the magnitude of this increase varied depending on the soil type. There was no significant correlation between the DTPA-extractable Zn and the uptake of this nutrient element by corn. There was a negative significant correlation (P < 0.05) between the equivalent calcium carbonate content and available P with the DTPA-extractable Zn. A significant linear relationship (R2 = 0.31) was found between the buffer capacity of soil for Zn and clay content. According to the results obtained from the stepwise regression analysis, the DTPA-extractable Zn and buffer capacity of soil for Zn were not correlated with other measured soil properties.

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Organic acids can affect phosphorus (P) sorption. In order to evaluate the effect of citric acid and oxalic acid on phosphorus sorption characteristics in calcareous soils, five different soil samples (0-30 cm) were collected in West Azerbaijan province. Soil samples (2.5 g) were equilibrated with solutions of KH2PO4 containing 0 to 20 mg P l-1. Five mM organic acids were added to each tube and sorbed P was calculated. The sorption data were fitted to Langmuir, Freundlich and linear equations. The results showed phosphorus sorption data were well described by the Freundlich and linear equations. The average values of Kf (distribution coefficient), n and equilibrium buffering capacity (EBC) of linear equation in citric acid treatment were reduced to 29, 12 and 17 percent, respectively and for oxalic acid reduced to 43, 27 and 14 percent, respectively. Maximum sorption was reduced significantly by oxalic as compared to citric acid. Standard phosphorus requirement (SPR) was decreased by 57 % as a result of organic acids additions compared to control condition. It is concluded that organic acids additions to soils containing more calcium carbonate and high amount of clay can decrease P sorption.

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Iron and manganese oxides as well as hydroxide minerals are among active constituents in soils because they are sensitive to environmental changes and often move frequently along soil profile. Therefore, their chemical forms content and their ratios are used as a soil developmental criterion. The present study was conducted in order to evaluate the effects of topography and drainage conditions on chemical forms of Fe and Mn along a soil catena in Dasht-e- Roum plain, in Kohgilouye Province. According to the results, maximum pedogenic Fe and Mn (Fed and Mnd) was found in more stable geomorphic surfaces. Higher values of Fed and Mnd were mostly observed in surface horizons compared to soil depth. Aquic soils exhibited higher contents of poorly crystalline Fe and Mn (Feo and Mno) and higher contents of Fed and Mnd. A significant correlation between clay content and Feo, Mno, Fet and Mnt contents was found. In addition, aquic condition increased Feo/Fed, Mno/Mnd and Mnd/Fed, 3.1, 4.3 and 1.9 times respectively but decreased the Fe crystallinity index 2.6 times. Aquic soils seem to have more favorable conditions for the formation of pedogenic Mn compared to pedogenic Fe, hence higher content of Mnd/Fed was observed in these soils

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In order to investigate the effects of municipal solid waste enriched with mineral fertilizers on some soil physical properties and lead (Pb) and chromium (Cr) concentration in plant organs of spinach, a field experiment was carried out in a factorial arrangement based on the randomized complete block design with three replications in 2008. The main plot included four levels of fertilizer, control without fertilizer, 20 tons of multiple solid waste + 50% chemical fertilizers and 40 tons of multiple solid waste + 50% chemical fertilizers per hectare and sub-plot consisted of a period of application in 3 levels of time (one, two and three years). The results showed that application of enriched municipal compost increased field capacity, soil porosity and moisture holding capacity, but particle and bulk density of soil decreased compared to the control. Also, the physical parameters in the two levels of emriched waste compost were higher than chemical fertilizer treatment. On the other hand, using for three-years the urban waste compost enriched fertilizer at all levels increased significantly (p 0.05) lead and chromium concentrations in spinach. The Pb and Cr accumulated in roots and shoots of spinach in urban using 40 tons of waste compost per hectare with 50 percent of chemical fertilizer for a period of three years and showed a significant increase. The amounts of fertilizer in all three levels were higher than the root element shoot.