JWSS - Journal of Water and Soil Science

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Any mechanical force exerted on a potato tuber during the harvesting may result in mechanical damage. A few weeks prior to harvesting, haulm destruction could improve the rheological properties of the tuber and reduce its susceptibility to mechanical damage. The effects of elevator and oscillatory sieve diggers and haulm destruction on potato loss and mechanical damage were studied on a farmer field in Fereidan region of Isfahan. Two haulm management treatments (destruction and non-destruction) along with five potato diggers (elevator digger with a long main web and with agitator, elevator digger with primary and secondary webs and with agitator, elevator digger with a main web and with/without agitator, and reciprocating riddle digger) were laid out in a strip block design within a randomized complete block design with four replications. The main crop variety, namely Marfona, was planted in the field.

The results showed that the percentages of undigged and soil-covered tubers were significantly different among the potato diggers tested. These losses were due to the dices located to the sides of shares or gage wheels and wrong adjustments on the diggers. Comparing the performance of reciprocating riddle with elevator diggers, its sieving capacity was low and the severity of the mechanical damage and the numbers of tubers covered with soil were high. Haulm destruction was effective in reducing the peeler type damage (the less than 3 mm deep damage to flesh). Although the elevator digger with a main web (elevator chain) and without agitator had the least mechanical damage index, the differences among the elevator diggers tested were not significant (5%).

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Muteh wildlife refuge, with an area of about 220,000 ha, is situated at 95 km north of Isfahan. In this refuge, 511 species of vascular plants belonging to 284 genera and 59 families live along with 117 species of vertebrate animals belonging to 84 genera and 37 families.

 Rare plants of the area were classified into two categories of “endangered” and “vulnerable” according to IUCN, 1996 categories. The endangered species include Astragalus chaetopodus Bunge. Astragalus eriostomus Bornm. Astragalus indistinctus Podl. and Maassoumi Cousinia phyllocephala Bornm. and Gauba Cousinia rhaphiocephala Rech. f. Cousinia straussii Hausskn. and C. Winkl. Ferula kashanica Rech. f. Onobrychis psoraleifolia Boiss. var. Psoraleifolia and var. pleiophylla Bornm. The vulnerable species include Bunium caroides (Boiss.) Hausskn. ex Bornm. Dielsiocharis kotschyi (Boiss.) O. E. Schultz Dionysia caespitosa (Duby) Boiss. Dorema ammoniacum D. Don. Dorema aucheri Boiss. Dracocephalum kotschyi Boiss. Echinophora platyloba DC. Hymenocrater bituminosus Fisch. and C. A. Mey. Pimpinella deverroides (Boiss.) Boiss. Prangos cheilanthifolia Boiss. and Thymus daenensis Celak subsp. daenensis.

 The rare animal species of the region were classified into four categories: “critically endangered”, “endangered”, “vulnerable”, and “conservation-dependent species”. The critically endangered species are Felis silvestris Schreber F. caracal Schreber Jaculus blanfordi Murray (mammals) Gypaetus barbatus Falco pelegrinoides F. cherrug and F. peregrinus (birds). The endangered species include Vulpes cana Blanford Ochotona rufescens Gray (mammals) Falco naumanni F. columbarius Chlamydotis undulata (birds) and Varanus griseus (reptilian). The vulnerable species are Gazella subgutturosa Guldensteadt Canis lupus Linnaeus Meles meles Linnaeus Hyaena hyaena Linnaeus (mammals) and Aquila chrysaetos (bird). And, finally, the conservation-dependent species include Ovis orientalis Linnaeus Capra aegagrus Elxleben Panthera pardus Linnaeus (mammals) and Ammoperdix griseogularis (bird). The results from this survey were compared with the findings from the survey of some other protected areas in Iran.

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In this study, the optimum irrigation depths for corn grain production under different conditions, i.e. maximum grain yield production and maximum benefit under limited land and water conditions, were determined under sprinkler irrigation in Bajgah (15 km. north of Shiraz).

 The results showed that, the optimum depth of irrigation for maximum grain yield production was 77.0 cm. Because of low price of irrigation water and sensitivity of corn crop to water deficit, the optimum depths of water were 76.8 and 73.4 cm under land and water limitations, respectively. Therefore, under limited water conditions, only 4.7 percent of the full irrigation water (maximum corn grain production) can be saved for maximum profits.

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A field survey was carried out in Firoozabad Beneh (Pistacia mutica) Forest Station covering about 12000 hectares. Data were collected using combined random and systematic sampling methods to study the effect of altitude, dominant slope direction, sex and age of the host and plant canopy on rust severity caused by Pileolaria terebinthi.

The rust severity decreased with plant age and elevations above 2000 meters. Female trees were more susceptible to the pathogen than males. The slope direction and plant canopy had no effect on disease severity. There was a significant correlation between plant age and elevation with disease severity but not with plant canopy. Regression analyses indicated a linear regression between disease severity variables and dependent variables of age, sex, and elevation.

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In the Islamic Republic of Iran, the occurrence of chilling and freezing stresses have frequently caused great damages to crops and horticultural products. In southern Fars Province (south Iran) the cultivation of citrus orchards is popular and the economic losses due to injury from chilling and freezing stresses may exceed billions of Rials annually. The drop of ambient air temperature (above zero) reduces the ordinary metabolism activity of plants and causes chilling stress. If the temperature drops below zero and remains there for a considerable time, intercellular freezing may occur. This process always kills the cells and provokes tissue injury. In the present study, the possibility of predicting daily minimum temperature using the dew point of a previous day measured at 18:30 was examined.

 It was found that the prediction of minimum temperature is possible if the dew points are modified on the basis of the air relative humidity. For the episodes that relative humidity varies from 45% to 55%, minimum temperature at day i+1 was found to be almost equal to the dew point on the previous day (day i). For the periods that relative humidity is above (below) this range, the minimum temperature on day i+1 was observed to be greater (lower) than the estimated dew point on day i.

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Over the past decades, numerous regime formulations, describing channel width, average flow depth and channel slope, have been proposed for gravel-bed rivers. Using the downstream hydraulic geometry measurements from 280 field channels, nine gravel-bed predictive formulations were compared. They are attributed to Bray, Chang, Hey and Thorne, Kellerhals, Neil, Parker and Simons, and Albertson. The comparison showed that the dominant discharge and the median grain size are not sufficient hydraulic variables to predict the channel geometry (width, depth, and especially channel slope). Seeking more complex formulations with the measured parameters, it was not possible to improve the existing formulations significantly without spurious influences of common variables.

 A second database is thus gathered from 19 laboratory-based boundary-layer measurements published in Kironoto and Graf (13) and Song et al. (19). In fact, the boundary-layer theory will respond to a fundamental question: are the existing available variables in literature not sufficient or the methods of their estimations are not appropriate? Based on the present study, the methods of their estimations should be changed. The second database allows proving the usefulness of the Shields parameter for the prediction of average flow depth and channel slope. Hence, using boundary-layer theory for prediction of Shields parameter and stable channel parameters is recommended.

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The effects of three kinds of organic matter on the physical characteristics of two soil series (Coarse Loamy, Mesic, Typic, Xerofluvents and Fine Mixed, Calcixerollic, Xerochrepts) were studied along with the measurement of maximum dry bulk density (MDBD) and the corresponding critical moisture content (CMC). Soil samples were taken from a depth of 0-20 cm and the percentage of organic matter and soil texture were determined. Three kinds of organic matters (peat, farmyard manure and filter press apple) were mixed with the soils at four levels (0, 4, 8 and 12% by mass), and were compacted using 10, 20 and 30 proctor hammer blows. The compaction tests on soils were carried out at different moisture contents. The present experiment, using factorial design, was randomly conducted in three replications. The results of this study showed that while the mean MDBD declined significantly (P<0.01) from 1.53 to 1.28 gr.cm^-3, the mean CMC increased from 23.32 to 33.28% as the organic matter increased from 0.12%. In all the experiments, CMC decreased with increasing compaction efforts, so that the effect of mixed organic matter and soil in terms of MDBD reduction and CMC increase was decreasing in the order, peat, farmyard manure and filter press apple. The clay loam soil showed lower values of MDBD but higher values of CMC as compared to the sandy loam. The significant interaction effect observed between incorporation of organic matter and soil compaction were meaningful. Finally an equation as to the relationship between MDBD and CMC was proposed for the purpose of mixing the three organic materials with soil.

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Nitrogen fertilizer application for corn (Zea mays L.) based on available N of soil can decrease the need to N fertilizer and, consequently, reduce the risk of environmental pollution, especially that of groundwater and drinking water. The objectives of this study were i) determining soil N0₃-N critical level at 4 to 6 leaf stage, ii) to study soil nitrate distribution, iii) determining N rate required for maximum corn grain yield, iv) measuring soil residual nitrate nitrogen after harvesting, and v) using hand chlorophyll meter to evaluate N status of corn. The experiment was conducted under field conditions in Bajgah and Kooshkak research stations (Fars Province) in 1996 using a split plot randomized complete block design with four replications. Main plots were control, 60, 120, and 180 kg N ha^-1 as urea. At 4 to 6 leaf stage each main plot was divided into two sub-plots and N was applied at 60 kg N ha^-1 rate to one sub-plot and the other sub-plot was left as control.

 Results showed that critical soil N0₃-N level at 4 to 6 leaf stage in 0-30 cm depth for 90 percent relative yield in Bajgah was 12-14 and for Kooshkak was 8-10 mg kg^-1 soil. The highest coefficient of determination between corn grain yield and soil N0₃-N in 0-30 cm depth for Bajgah was in furrow sample and for Kooshkak was in furrow and shoulder composite sample. In both sites, maximum corn grain yield, with 15.5% moisture content, was about 14 mg ha^-1 that was obtained through the application of 120 kg N ha^-1 as preplant plus 60 kg N ha^-1 as sidedress which is about 1/2 of the application rate used by local farmers. The highest residual soil N0₃-N after harvesting was 24 and 18 and the lowest was 2.6 and 3 mg kg^-1 soil for Bajgah and Kooshkak soils, respectively. When maximum corn grain yield was obtained, chlorophyll meter reading at the middle of leaf blade at dough stage, was 49 for both sites. It seems that N fertilizer application rate by local farmers is excessive.

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Potassium fixation and release by phillosilicate clay minerals in soils are very important processes influencing the availability of K to plants. This investigation was conducted to determine the potassium fixation capacity and charge characteristics of soil clays of 15 surface soils (0-30 cm) from central and northern Iran. After clay particle separation, both total and tetrahedral cation exchange capacity of soil clays were determined. Tetrahedral CEC was measured after saturation with Li and heating at 300 C to reduce octahedral charge to near zero. Potassium fixation was obtained in both wet (1:10 soil:solution, 16 h on a shaker) and dry conditions (after drying for 24 h at 70°C) using three different levels of added K

The total CEC in soil clays of Isfahan, Char-Mahal and Gilan provinces ranged from 22.1-36.0, 33.0-55.8 and 31.3-47.9 cmol kg^-1, respectively. Tetrahedral CEC in soil clays of Isfahan, Char-Mahal and Gilan provinces ranged from 17.9-4504, 26.2-32.5 and 8.3-23.8 cmol kg^-1, respectively, which consisted of 81.0-98.4, 58.5-95.8 and 24.7-72.5% of their total charge, respectively. The amount of K fixation increased with drying and the level of k added. Mean potassium fixation in soil clays of Isfahan, Char-Mahal and Gilan ranged from 5.42-9.13, 6.63-14.67 and 8.87-10.36, respectively. Mean potassium fixation by soil clays (except for soil clays of Gilan) best correlated with total CEC. In the soil clays of Isfahan, mean potassium fixation correlated with tetrahedral CEC, whereas no correlation was observed in soil clays from other places. The average amount of potassium fixation in clay fractions was in the order: Gilan clays > Char-Mahal clays > Isfahan clays.

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The urease enzyme plays an important role in the efficient use of urea fertilizer and some environmental risk assessment. Urease activities in 20 different soil samples of arid to semi-arid regions of Isfahan Province were determined and their correlations with some soil physical, chemical and biological characteristics were studied. Urease activities range from 5.3 to 79.2 µg NH4⁺ g^-1 soil 2hr^-1.

Results indicated that soil organic carbon was significantly correlated with urease activity (r=0.899***). None of sand, silt and clay percentages were significantly correlated with urease activity. Total nitrogen was significantly correlated with urease activity (r=0.797***). Electrical conductivity of saturated paste extracts were also negatively correlated (r=-0.499*) but sodium adsorption ratio (SAR), pH, equivalent calcium carbonate and cation exchange capacity failed to be correlated significantly with urease activity. No significant correlations were found between urease activity and total bacteria (on nutrient agar) or total fungi (on potato dextrose agar), but the bacteria that could colonize urea-agar media were significantly correlated with urease activity (r=0.47*). Multiple stepwise regression analysis showed that organic carbon accounted for most of the variation in urease activity.

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To determine the relationships between yield and its components, and to find the direct and indirect effects of yield-related traits on soybean yield, 240 genotypes were grown in the Research Station, College of Agriculture, Karaj, Iran, in 1997-98 using an augmented design. The study of correlation analysis showed that number of seeds per plant and seed yield per plant had the highest significant correlation coefficient (r=0.92). Other significant correlation coefficients were found between biomass per plant and yield (r=0.86) and between number of pods per plant and yield (r=0.67). Results of stepwise regression analysis revealed that number of seeds per plant, seed weight, and number of seeds per pod were the three major traits affecting seed yield in soybean. However, path analysis showed that only two of the three above-mentioned traits, namely, number of seeds per plant and seed weight, are quite important for soybean selection programs. Stepwise regression analysis was used again, omitting number of seeds per plant, which showed that number of pods per plant, seed weight, plant height and number of days to 90% maturity, are important contributors to yield. Path analysis, this time, revealed that the correlation effects to plant height and number of days to 90% maturity on yield is due to the indirect effects exerted through other traits. It is concluded that three traits, namely, number of seeds per plant, seed weight and number of pods per plant have notable effects on soybean seed yield.

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The effect of N levels and planting densities on quantitative and qualitative characteristics of grain yield in grain sorghum (cv. Kimia) was studied in summer 1998 at Kushkak Agricultural Research Station, Shiraz University. The experiment was a spilt plot with 4 replications in which 4 levels of planting densities (7.7, 10, 15.4, 20 seeds m^-2) were applied as main plots and 4 levels of N (0, 40, 80, 120 Kg N ha^-1) as subplots.

 N application rates up to 120 kg N ha^-1 increased grain yield (7240 kg ha^-1), but due to increased planting density (5336 kg ha^-1) the highest yield obtained from 15 seeds m^-2. Interaction of N and planting density was also significant. Application of 80 kg N ha^-1 and highest planting density produced 8702 kg ha^-1 of grain. Different grain yields in different levels of N and planting densities were due to increased number of grains per basic branches of panicle and increased number of panicle per m^-2. The trend of variation of crop growth rates (CGR) was similar to the trend of grain yield. Nitrogen application increased grain protein contents but planting density had no significant effect on grain protein. Phenolic compounds (tannins) decreased with increasing N application and with decreasing planting density. A negative correlation was observed between increased N content and increased phenolic compounds.

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In order to investigate the genetic diversity of morphological traits in 121 genotypes of lima, red and pinto beans, and to study the relation between characters, and to get better understanding of factors affecting the interaction between characters by multivariate analysis, an experiment was conducted in 1997 as a simple lattice design at the Research Station of Agricultural College, Isfahan University of Technology.

High variability was observed among genotypes for all characters studied. The phenotypic coefficients of variability were greater than the genotypic ones for all the traits. Among the characters studied seed yield, number of pods per lateral branches and main stem, length of lateral branches and main stem, number of nodes per lateral branches and main stem, 100-seed weight and number of lateral branches had the highest genotypic and phenotypic variability, while days to maturity had the lowest variability. Traits relative to lateral branches were more variable than the same traits in the main stem. Factor analysis for all the genotypes and, separately, for lima, red, pinto, determinate and indeterminate genotypes revealed 4 factors which justified more than 78.4 percent of the total variation. The results of the analysis based on all the genotypes showed that the first factor was mostly correlated to days to maturity, length of main stem and lateral branches, number of nodes per main stem and lateral branches, which was named “vegetative factor”. The second and third factors had the highest correlations with number of pods per main stem and lateral branches, number of seeds per pod in the main stem and branches and 100-seed weight, which were named “yield component factors”. The fourth factor showed the highest correlation with number of lateral branches and was named for this trait. The first and fourth factors were related to vegetative growth characteristics and physiological source. The second and third factors were related to physiological sink. On the basis of stepwise regression analysis, number of pods per lateral branches was the most important component of yield, and number of pods per lateral branches and main stem had the next following ranks. Number of pods per lateral branches and main stem had the highest relationships with yield.

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An experiment was carried out on 384 7d old commercial broiler chicks (Lohman) to study the effect of changes in source and level of dietary calcium and limestone particle size on the performance and tibial growth and calcification of broiler chicks. From day 1 to day 7, the chicks were fed with the commercial basal diet. On day 7, the chicks were weighted and randomly assigned to 64 groups, six chicks of similar body weight (82.2±5.4) per group. Each of the eight experimental diets was fed to eight groups of chicks for an experimental period of 49 days. Two calcium sources (oyster shell and limestone), two calcium levels (100% NRC and 85% NRC) and three particle sizes of limestone [ground (less than 0.15 mm), medium (0.6 to 1.18 mm) and coarse (1.18 to 4.75 mm)] were added to diets with the same energy based on a Randomized Complete Design, in which levels of calcium were nested into calcium sources. Particle sizes were nested into calcium sources and levels.

Limestone significantly increased phosphorus retention (in percentage of phosphorus ingestion) at the age of 21 and 56 days and increased tibial length, dry weight and ash weight at 56 days. Calcium level of 85% NRC significantly decreased feed intake and feed conversion, especially during the starter, and increased calcium retention (in percentage of calcium ingestion) at 21 and 42 days. Phosphorus retention and cortical thickness of tibia at 21 and 42 days, tibial dry weight, ash weight and ash percentage at 42 day, were significantly increased with calcium level of 100% NRC. Coarse and medium particle sizes of limestone as compared to ground particle size, significantly improved feed intake and feed conversion, especially during the starter. Medium particle size of limestone with calcium level of 85% NRC and coarse particle size of limestone with calcium level of 100% NRC, significantly improved tibia ossification characteristics and increased calcium and phosphorus retention. By using calcium level of 85% NRC, coarse and medium particle sizes of limestone compared to ground particle size, significantly diminished percentage of leg abnormalities.

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Cooperative have always been considered institutions capable of solving major problems in the process of development in different countries. These institutions has evolved along with theories of development from government dependent organization to independent, people centered with the objective of being equal-opportunity systems for all members in order to be responsive to current goals of development. The aim of the present research was to determine the level of success in mechanized companies in Fars Province (12 Mechanized Companies, 24 Rural Cooperatives and 8 Production Cooperatives) in achieving development goals through a survey research.

The findings indicated that production cooperatives were more successful in promoting farmers' income and participation of members in community activities, increasing equity and farmers' work related welfare while mechanized companies had more achievements in flexibility and participation of members in making decisions regarding farming activities and in terms of payment to farmers. In other words, production cooperatives have been more successful in achieving development goals (increasing income, participation, and work related welfare and decreasing inequity). Mechanized companies rank a position in the middle of the success scale, but rural cooperatives had the lowest success level in achieving development goals.

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Effects of the β-adrenergic agonist metaproterenol were studied daily gain, carcass composition and feed consumption of Varamini female lambs were studied. Thirty lambs were randomly assigned to a randomized complete block design with three groups (treatments) and two blocks (live weight). The ration was similar for all groups (ME: 2.6 MCal/kg, CP: 14.9%) and lambs were fed ad libitum. Metaproterenol was injected subcutaneously at 0 (control), 7 (low dose) and 14 (high dose) µg kg^-1 w⁷⁵. Lambs were weighed at 20-day intervals.

Mean daily weight gain and feed conversion ratio improved significantly by metaproterenol (P<0.05), but feed intake was not affected. Hot carcass weight, carcass weight minus fat-tail, dressing percentage and longissimus muscle characteristics were different among the three groups (P<0.01). Physically separated fat from several depots and fat-tail weight significantly reduced in the metaproterenol groups. Carcass fat content decreased but carcass protein content increased in metaproterenol treated lambs.

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Three levels of urea (0, 0.5 and 0.75% on fresh weight basis) were added to whole corn plants to investigate their effects on the chemical composition, and digestibility in 16 Ghezel male lambs using a completely randomized design. Rumen degradability and nutritive values were also evaluated.

 pH, total nitrogen and ammonia-N concentration of silages increased (P<0.05) with the addition of urea. Total nitrogen apparent digestibility was higher (P<0.05) with the urea addition. Nitrogen balance in ruminal fluid increased (P<0.05) with the addition of 0.5% urea. pH and ammonia-N concentration increased (P<0.05) with the addition of urea until 1.5 hr post feeding. Blood serum urea nitrogen concentration was higher (P<0.05) with the addition of urea until 6 hr post feeding. Potential degradability of dry matter and organic matter was higher at 0.5% urea level. Average daily gain increased with the urea addition and was highest (212.88 gr) at 0.75% urea. Subcutaneous fat depth was significantly (P<0.0l) lower for the sheep fed with 0.5% urea-treated silage as compared to other treatments. The results indicated that feed efficiency may be improved by urea addition and that the addition of urea (0.5% on fresh weight basis) may be recommended in order to increase the feeding value of whole corn plants.

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The quality of Iranian breads has intensively decreased during recent years due to socio-economic factors and poor quality of flour. The purpose of this study is to investigate the effects of mono- and diglycerides and ascorbic acid as improvers on Iranian bread (Taftoon) quality. Three wheat type flours (Ghods, Mahdavi and Roshan wheat varieties produced in Isfahan region) were used. The effects of ascorbic acid at three levels (20, 40 and 60 ppm flour basis) and esters of mono- and diglycerides at three levels (0.5, 1.0 and 1.5 percent) on the dough baking and bread quality were measured. The Taftoon bread was baked in a semi-traditional oven.

 Loaves of bread were scored after baking and also on the first and second days of storage at room temperature. Analysis of variances indicated that ascorbic acid and mono- and diglycerides have significant effects on the improvement of the rheological properties of dough. However, ascorbic acid had higher effects than mono- and diglycerides. The results of the tests showed that dough resistance to mixing and tensile stress increased with addition of the improvers. Both improvers used in this experiment have an anti-stabling effect on bread. However, the ascorbic acid effect is much less than that of mono- and diglyceride. The low concentration of 0.5 percent of mono- and diglyceride and 60 ppm of ascorbic acid with flours of moderate protein contents (10-11%) resulted in good quality Taftoon bread with good organoleptic and tearing qualities after two days.

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In this study, the control method of greater wax moth using male sterilization technique with gamma ray and chemical control methods were examined and compared. In order to determine the safe and effective dosage of gamma-ray to sterilize male pupae of the greater wax moth, an experiment was conducted in a complete randomized design with 5 treatments and 3 replicates with 50 male pupae in each replicate. Treatments included different levels of gamma-ray dosages with zero, 250, 300, 350 and 400 gray.

Release ratio of sterile to normal males was also studied in a similar experiment. Treatments included sterile-males, normal males and virgin females with the ratios of 1:1:1, 2:1:1, 3:1:1, 4:1:1 and 5:1:1. In this study, male pupae were irradiated with 350 grays of gamma-ray. The possible parthenogenetic reproduction of this pest was also studied. In order to compare the results from the male-sterile technique and the chemical control of this pest, another experiment was conducted in wax stores using 3 treatments with 3 replicates. Phostoxin was used as the most common chemical pesticide with a dosage of 1 tablet of 3 grams of 56% per 1 m3 space for the control of this pest.

The results showed that the best dosage to sterilize male pupae of the greater wax moth was 350 grays of gamma-ray. Also the best release ratio was four sterile male, one normal male for each normal female (4:1:1). Our results indicated that the females of the greater wax moth were not able to reproduce through parthenogenesis. There was no significant difference between the chemical and male-sterile methods for the control of this pest.

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Regarding the importance and high potential of Encarsia formosa Gahan (Hymenoptera: Aphelinidae) for control of the greenhouse whitefly, Trialeurodes vaporariorum Westwood (Homoptera: Aleyrodidae), the effect of different diets and storage of the parasitoid at low temperatures on its longevity and efficiency was studied. The average longevity of the parasitoid on different diets including honey-syrup 15%, honey-syrup 10%, sucrose syrup 15%, honeydew of whitefly were significantly different at 1% from distilled water and control (without water and food) treatments.

Change in honey-syrup concentration at a range of 5% did not affect the longevity of the parasitoid. The honeydew and sucrose-syrup treatments were not significantly different. This was probably due to the relative similarity of food quality of these two diets. Storage of host parasitized nymphs containing pupae of 1-2 days-old of E. formosa at 8±1°C affected the emergence rate and efficiency of adult parasitoids.

Four treatments including the pupae of parasitoids in 4th nymphal instars of the greenhouse whitefly were stored at 8±1°C for 5, 15, 25 and 35 days. Control treatment included pupae of parasitoid that were held at room temperature, 24±4°C. Host nymphs were parasitized by the parasitoids emerging from all treatments. The average parasitized nymphs by parasitoids emerging from 5, 15 days and control treatments were not significantly different at 1 %.