JWSS - Journal of Water and Soil Science

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This study was conducted to evaluate the effect of salinity on concentration of potassium (K), sodium (Na) and chloride (Cl) ions, in sweet lime budded on five citrus rootstocks, including Bakraii (mandarin x sweet lime), Volcameriana, Sour orange, Sweet lime and Mexican lime in a glasshouse, using a randomized completely design with factorial arrangement and four replications. Rootstocks had great effect on the concentration of ions in scion. Concentration and distribution of ions were significantly different in control and other treatments. Salinity increased Na and Cl ions in shoots and roots, but the rate of increase varied among rootstocks and treatments. Lowest concentration of Na and Cl ions were in shoot of scion on Volkameriana. Under salinity stress, K concentration increased in shoots of scion on Sour orange and Bakraei and decreased it on other rootstocks. Salinity increased K concentration in roots of all rootstocks except for Mexican lime.

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In order to study the physiological responses of two barley cultivars to salinity stress, a 4-replicate CRD greenhouse experiment was conducted during at the College of Agriculture, Shiraz University, Iran2003-2004. The treatments consisted of two barley cultivars: Afzal and Reyhan and five salinity levels: 0, 4, 8, 12 and 14 dS/m. Seedling emergence, number of tillers and leaves per plant, leaf area and dry matter decreased with increasing salinity levels. The reductions were lower in Afzal (salt tolerant) than Reyhan (salt sensitive). A lower Na⁺ and higher K⁺Na⁺ in Afzal compared to Reyhan were observed under high level of salinity. The results of the present experiment also showed that the tolerance to salinity was associated with a greater stomatal conductivity, as well as transpiration and photosynthesis rate. Sub-stomatal CO2 concentration in both cultivars decreased with an increase in salinity, with the exception of 12dS/m, where it led to an increase in sub-stomatal CO2 concentration. Leaf temperature increased with an increase in salinity level in both cultivars, whereas a more pronounced decrease for number of fertile tillers, ears per plant, kernels per ear, thousand grain weight and harvest index was observed in Reyhan compared to Afzal .Since a higher tolerance to salinity in Afzal was apparently associated with a lower concentration of Na⁺ and a higher K⁺/Na⁺ ratio of the shoots, this trait could probably be used for yield improvement of barley cultivars under saline conditions.

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Inheritance of physiologicaly related salt tolerance traits including Na⁺ and K⁺ contents, K⁺/Na⁺ ratio of young leaves and biological yield (BY) in six basic generations (P₁, P₂, F₁, F₂, Bc₁ and Bc₂) and their reciprocal crosses derived from crosses between Kharchia × Niknejad and Shorawaki × Niknejad were studied in sand culture under high salinity treatment (EC = 22.5 dS m^-1). Generation means analysis indicated that a simple genetic model (including additive and dominance effects) is sufficient for Na⁺ and BY in Kharchia × Niknejad cross and for Na⁺, K⁺/Na⁺ ratio and BY in Shorawaki × Niknejad cross but, for other traits digenic interactions (additive × additive and dominance × dominance) were important parameters in the expression of salt tolerance of the various generations. Dominance genetic effects were predominant genetic components in most of the models. Weighted generation variances analysis suggested that dominance variance component was more important for Na⁺ and K⁺ content in both crosses. This result was confirmed by significant differences between back cross generations. Results also showed negligible dominance for K⁺/Na⁺ ratio in both crosses, multidirectional dominance for BY in Kharchia × Niknejad cross and absence of dominance gene action in Shorawaki × Niknejad. Kharchia × Niknejad cross showed, in general, more genetic variation, broad-and narrow sense heritabilities than Shorawaki × Niknejad cross, indicating the important role of primary differences between parents. These results revealed that recurrent selection followed by pedigree breeding or a selective diallel mating system may prove useful in improving salinity tolerance of wheat plants. The involvement of dominance × dominance interactions for some traits indicates that it is necessary to postpone selection for salt tolerance of wheat to advanced generations, when sufficient epistatic interactions have become fixed.

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The influence of Nitrogen (N) rates on mineral nutrient uptake in stem and seeds, proline and carbohydrate in flag leaves of Wheat (Triticum aestivum L . Var Chamran) under saline conditions was studied in a field experiment in 2003 and 2004. The experiment was conducted using a split plot design with three replications. The treatments comprised five levels of salinity: 1.5, 5, 10, 15 and 20 ds/m in main plot and three nitrogen levels: 50 , 100 and 150 kg N/ha in sub plot. Salinity treatments were applied in a clay–loam soil by water with NaCl and CaCl₂ (5:1 by wt ). The results showed, the nutrient uptake was influenced by both salinity and N treatments. With the Exception of magnesium in seed, salinity increased nitrogen, calcium and magnesium concentrations in seed and stem in both years. By increasing salinity levels, the concentration of potassium in stem and seed decreased and Sodium concentration increased. In the stem the concentration of Sodium in the 20 ds/m was about 17 and 22 times more in the first and second year, respectively. In these experiments, by increasing salinity and nitrogen treatments, proline concentration in flag leaves increased in the two stages (flowering and milky stages) in both years. Salinity had similar effect on carbohydrate accumulation in both stages, but nitrogen treatment had two different effects on carohydrate concentration. In flowering stage, by increasing nitrogen application, carohydrate concentration increased but in milky stage decreased.

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In order to study the effects of salinity on germination, vegetative growth and some physiological characteristics of canola cultivars, two experiments were conducted at Research Station of Agricultural College of University of Kerman in 2004. The experimental design was a Completely Randomized Block with four replications. Treatments comprised all combinations of different levels of three factors including variety (Kobra × Regent, Ceres and Okapi), salt type (NaCl, CaCl₂) and electrical conductivity of saline solution (0, 4, 8, 12 dS/m). Results of variance analysis showed that salinity has a very significant effect on germination percentage, germination homogeneity, germination rate, rootlet and polomul length at the end of the germination stage. Shoot length, dry weight, diameter and number of nodes per shoot were significantly affected also by salinity at vegetative growth. Regarding the two types of salt, none of the measured traits were significantly different. All the mentioned traits decreased with increasing salinity the highest and the lowest values were recorded at control and at 12 dS/m salinity levels. Membrane ion leakage was the only trait which increased significantly. Comparison of means showed germination percentage and germination homogeneity at germination stage and the number of nodes at vegetative growth stage were affected less than other traits by salinity, and they only decreased significantly with increasing salinity to 12dS/m. The effect of cultivar on these traits was also significant (P<0.001). In both growth stages, cultivars response to salinity was different. At germination stage, Ceres and Kobra * Regent cultivars were the most sensitive and tolerant ones in terms of all traits and there was no significant difference between Okapi and Kobra *Regent. At vegetative growth stage, however, Okapi showed less growth than Kobra * Regent and their difference was significant. It seems that evaluation of traits response at germination stage can not be effective to determine salt tolerance of canola cultivars.

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  Sugarcane is one of the most important sugar crops in the world. Because of semi-arid climate and salinity of its cultivation area in our country, increasing salt tolerance of sugarcane is signifying. To achieve this goal determining salt tolerant cultivars and understanding salinity mechanisms in sugarcane are very important. This study was conducted to evaluate 8 commercial and promising sugarcane cultivars at early stage of growth. A complete randomized design with three replicates and four salinity treatments (0, 0.25, 0.5, 0.75 % NaCl) was used in a hydroponics system. The effect of salinity on absorption, transport and accumulation of Na⁺, Cl^- , K⁺ and Ca²⁺ ions in shoot and root was determined. At high level salt concentration, Cl^- content in shoot and root increased. Result showed that sodium accumulation in sugarcane plants was more than potassium. By increasing salinity level, sodium uptake and its translocation to shoots increased reducing growth and dry matter yield of plants. With rising salt concentration from medium (0.5%) to high (0.75%), content of chloride in shoot and root of NCO-310 was constant showed that this cultivar had genetic ability to avoid Cl^- uptake. CP82-1592 with lowest ratio of shoot / root chloride had minimum transport of Cl^- to shoots. Also this cultivar had high content of Ca²⁺ in shoot and low Na⁺/Ca²⁺ ratio at all salinity levels. CP48-103 had low sodium in shoot and relatively low sodium in root. Thus it probably has genetic potential to avoid sodium uptake. At last, exclusion of Na⁺ and Cl^- to older leaves and tillers was seen in CP82-1592 and CP72-2086 cultivars. According to results, to avoid once of absorption and transport, and exclusion of harmful Na⁺ and Cl^- ions were mechanisms that could be used in salinity tolerance of sugarcane.

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  This research was carried out to study the impacts of geomorphologic characteristics of claypan on land use and land degradation. Databank of the study area was constructed and digital terrain model of claypan was prepared. By using GIS techniques spatial distributions of the subsurface drainage network, sediment transportation index and wetness index were calculated. The results indicate that the depth to the claypan is between 0 (where the pan is exposed at the surface) to 605 cm. There are several depressions in the claypan which are filled by new sediments. Each depression has a catchment which is charged by the drainage water of its attributed lands. While a depression drains naturally or synthetically, the attributed soils over the depression are in non saline or moderate salinity condition otherwise, a marshland, a waterlogging area or a salt crust zone develops over there. The results also indicated that soil surface salinity is a function of depth to claypan and drainage condition of area. The trends of salinity extension are different for closed and open catchments and the depth to the claypan could be estimated using electrical conductivity. The results also show that analysis of microtopography of soil surface and soil stratification should be considered for designing irrigation and drainage networks.

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  Effect of change from the conventional (flooding) to subsurface irrigation system was studied on mature pistachio trees (Cultivar Ohadi) in Kerman pistachio research station for four years. This experiment was carried out in a randomized complete block design including 7 treatments and 3 replications. The experiment also used two irrigation intervals (7&14 days) and three water rates according to pan coefficient (20%, 40%& 60% evaporation from surface of class A pan) for subsurface irrigation system with 6 treatments the conventional (flooding) irrigation system was tested as control. The pipes of subsurface irrigation system were installed at 90cm distance from trees trunk at each side of trees, and 50cm from soil surface. Mean of pressure and discharge in the pipes was 0.8 to 1 atmosphere and 5.4 Lh^-1m^-1, respectively. Different factors of quantity and quality pistachio yield, current vegetative growth, water use efficiency and soil electrical conductivity were measured. The results showed that change of conventional (flooding) to subsurface irrigation system is possible and the best treatment can have water rate of 7174 m³ha^-1y^-1 with 14 days irrigation interval. In comparison with other treatments, this treatment has better quantity and quality pistachio yield. Water use efficiency (WUE) of this treatment was 164g dry yield per cubic meter. Nevertheless, application of water rate of 4783 m³ha^-1y^-1 can result in the deficiency of irrigation water. At this water rate, WUE was 177g dry yield per cubic meter. However, it was found that the application of lower water rate (<4783 m³ha^-1y^-1) produced severe effects on the treated pistachio trees, e.g. decreasing the growth rate, yield and increasing the early splitting in pistachio fruits. Finally, it is concluded that 14-day interval irrigation is the preferred treatment for pistachio trees. Also, soil salinity showed considerable change in subsurface irrigation treatments after irrigation system was changed, especially in the first year.

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To study the effect of salt stress on quantitative and qualitative parameters of canola (Brassica napuse. L) cultivars, and determine the possible mechanisms of salt tolerance and the best salt tolerance indices, an experiment was conducted under a rain-shelter at experimental field of university of Kerman in 2004. The experimental design was a complete randomized block (RCOD) with four replications. Treatments consisted of all combinations of different levels of three factors including variety (Kobra×Regent, Ceres and Okapi), salt types (NaCl and CaCl₂) and electrical conductivity of saline solution (0, 4, 8 and 12 dS/m). All treatments were randomly distributed in each replication. Results of the analysis of variance showed that grain yield, yield components, oil and protein contents were significantly affected by salinity (P< 0.001). All the mentioned traits reduced with increasing salinity. It was also found that there were significant differences among genotypes for the mentioned traits. Cultivar of Kobra×Regent showed the highest values of the above traits except for the oil content. Proline, protein and sugar contents were also affected significantly by salinity (P<0.001). The highest and lowest sugar concentrations and protein contents were recorded for control and 12dS/m treatments, respectively. Proline content showed a reverse response and increased with increasing salinity. Mean productivity (MP), geometric mean productivity (GMP) and stress tolerance index (STI) are suggested to be the most suitable indices in order to select genotypes of high productivity under both stress and normal conditions. Based on these results, Kobra*Regent was selected as the most tolerant cultivar.

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Canola (Brassica napus L.) in response to salinity represents various resistances with respect to its phonologic stages. Most plants such as Canola are resistant at germination stage. However, at seedling or earlier growth stages, plants become more sensitive to salinity but their tolerance increases with age. Salt tolerance of various plants has been extensively studied however, the results have either been qualitative or expressed as average values over root zone salinity for the whole growth season. Thus, developing appropriate models for quantitative characterization of plant response to salinity at different growth stages is essential. Canola which is considered as high economic value plant was selected for this study. Two productive stages for canola are recognized as flowering and ripening. To determine the effect of salinity on canola at vegetative growth stages, a greenhouse experiment was conducted on a natural saline loamy sand soil, using salinity treatment including one non-saline water (tap water) and 8 saline waters of 3 to 17 dS.m^-1. The canola plants were irrigated with tap water before the desired stage and then salinity treatments were imposed. The Maas and Hoffman (1977), van Genuchten and Hoffman (1984), Dirksen et al., (1993), and Homaee et al., (2002b) models were used to predict relative transpiration (T_a/T_p ) and relative yield ( Y/Y_m) as a function of soil salinity. The maximum error (ME), root mean square error (RMSE), coefficient of determination (CD), modeling efficiency (EF) and coefficient of residual mass (CRM) statistics were calculated to compare the models and their efficiencies. The results indicated that the van Genuchten and Hoffman (1984) model provides best prediction at flowering stage. However the Homaee et al. (2002b) model offers better prediction at ripening growth stage.

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Soil salinity is an increasing environmental stress on crops in most areas of Iran since farmers use underground saline water for irrigation. In order to investigate the effects of salt stress on germination and seedling growth of safflower (Carthamus tinctorius L.), an experiment was conducted at two stages (germination seedling growth), using four levels of NaCl salinity (0, 5, 10, 15 ds/m), and four cultivars of spiny and non-spiny safflower ( a landrace from Isfahan bred cultivars of IL, LRV and PI). The design was factorial and completely randomized based on 3- replicates (CRD). Germination percentage, germination velocity index, root and shoot length, root and shoot dry weight, root to shoot dry weight ratio and salt tolerance index were measured at germination stage. In seedling growth stage, traits such as, stem length, number of leaves, chlorophyll index, root and shoot dry weight, root to shoot dry weight ratio and salt tolerance index were also measured. Results for both growth stages showed that salt stress decreased all the above mentioned traits, significantly, in all cultivars. Salinity × variety interaction at germination stage was also significant, compared to seedling growth stage. Salt tolerance index decreased significantly with increasing salinity at both stages, but differences between cultivars were not significant. Cultivars PI and IL were characterized as the tolerant ones at germination and seedling growth stages, respectively. It could be concluded that safflower cultivars responded differently to salinity in germination phase compared to seedling growth phase, and that safflower suffers more from salinity during germination stage.

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Salinization and alkalization are considered spatiotemporal dynamic soil degradation processes. In order to investigate the effects of agricultural activities on land degradation and soil salinity, Shamsabad area in Qom province was selected. Aerial photos (1955) and satellite images (1990-2002) were used to examine the changes in land use. Soil samples were collected from 25 locations in the study area from 0-50 cm and 51-100 cm depth at each location. For comparative purposes, sampling locations in this study were similar to locations used for salinity study in 1983. For each sample, pH, electrical conductivity (ECe), base saturation percentage, exchangeable sodium, lime and texture were measured. Land use and salinity maps were created by using geographic information system (GIS) softwares. Results revealed an increase of 9.5 times in cultivated lands in 47 years. Increase in agricultural activities in the study area has also intensified the pressure on water resource in the area, lowering ground water tables and degrading water quality. In the 0-50 cm soil depth, the average soil ECe was 6.5 dS/m in 1983, which increased to 10.7 dS/m in 2005. If soil salinity trend and pressure on water resources continue, large part of Shamsabad area is expected to change to desert in near future years.

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Modeling plant response to salinity and nitrogen deficiency is very important for estimating optimum yield in arid and semi-arid regions. For this purpose, the models of Leibig-Sprengel (LS) and Mitscherlich-Baule (MB) originally proposed to explain plant response to nutrients only were modified to evaluate plant yield response to combined nitrogen and salinity stress conditions. Afterwards, in order to model canola (Brassica napus L.) response to combined salinity and nitrogen stress, an experiment was designed with different nitrogen and salinity levels. The water salinity treatments consisted of non-saline water, 3, 6, 9 and 12 dS m-1. The nitrogen treatments were 0, 75, 150 and 300 mg kg soil-1 added as ammonium nitrate. The results indicated that both modified models can satisfactorily predict canola yield. However, the modified MB model (R2=0.94) provided better estimation than the modified LS model (R2=0.87). The calculated statistics including Maximum Error, Root Mean Square Error, Modeling Efficiency, Coefficient of Determination and Coefficient of Residual Mass for the modified models indicated that the estimated relative grain yield for soil nitrogen, salinity and each rate of soil nitrogen in salinity levels by modified MB model compared with those by modified LS model is closer to the measured relative yield. Therefore, the use of modified MB model for estimating canola relative grain yield in salinity and nitrogen stresses is recommended. Using modified LS model showed that the salinity threshold value changes with the applied nitrogen. In this case, by application of each 75 mgN kg-1 soil, the salinity threshold value decreased 4 dS m-1 in saline conditions. Application of nitrogen decreased chloride concentration in grains under saline conditions. Nitrogen uptake also augmented with increasing canola transpiration, because nitrogen was carried over from soil to the uptake sites mainly by mass flow.

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To evaluate effects of different salinity levels on activity of antioxidant enzymes (CAT, APX and GPX) and osmotic components (carbohydrate and proline) in two sorghum genotypes, an experiment was conducted as completely randomized factorial design with three replications in Zabol university in 2007. Three levels of salinity control (0), 100 and 200 mM NaCl and two sorghum genotypes (Payam and Sistan) were compared. Results showed that by increasing salinity levels from 0 to 200 mM NaCl, the activities of these three antioxidant enzymes were significantly increased and the highest level of activity was observed in 200 mM salinity. Payam cultivar had the highest level of APX and GPX enzyme activity in these experiments. salinity significantly changed two osmotic adjustment concentrations (Carbohydrate and proline) and increased their concentrations. The highest proline and carbohydrate were showN in Sistan and Payam, respectively. In this study we found positive correlation between osmotic components and antioxidant enzyme activity. The results showed that in these two sorghum cultivars the two mechanisms help with salinity tolerance. A direct relationship between activity of the antioxidant- enzymes and accumulation of osmotic regulators (carbohydrate and proline) was observed. In Payam genotype, as the activity of the enzymes and accumulation of osmotic regulators increased, the weight of the plants decreased. The decrease in weight could mean some of plant energy is allocated to the production of antioxidant enzymes and osmotic regulators.

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In order to study the effect of NaCl priming on some agronomical and physiological traits of corn (single cross 704), an experiment was conducted at Agricultural Research Station of Bahonar University of Kerman in 2005. The experimental design was completely randomized designs with 3 replications. Treatments were a combination of all different levels of two factors including salinity levels (1, 4, 8, 12, 16 dS/m) and salinity solution for NaCl priming (1, 4, 8, 12, 16 dS/m). Results showed that salinity and NaCl priming had significant effects on total emergence, mean time to emergence, root and stem length, number of leaf, relative water content, ion leakage and K+/Na+. Mean comparison showed that increasing salinity decreased all plant characteristics (with the exception of mean time to emergence and ion leakage) by %49.5, %33.49, %23.97, %18.64, %14.05 and %40.20, respectively. However, increasing salinity led to 1.2 and 1.3 increase in mean time to emergence and ion leakage of leaves, respectively. Mean comparison also showed that NaCl priming decreased negative effects of salt stress, and all mentioned traits under NaCl priming increased as compared with control. The results showed that NaCl priming is a useful method for increasing salt tolerance in corn plant.

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In order to study the effects of salt stress and leaf feeding by ascorbic acid on antioxidant enzyme activity, proline accumulation and lipid peroxidation on leaves and roots of Brassica napus L. cvs. Okapi, an experiment was conducted as a factorial in a completely random design with three replications. Plants were exposed to salt stresses by NaCl solution (200 mM) and foliar were treated by ascorbic acid solution (25mM). Catalase, Superoxide dismutase and peroxidase activity were assayed in shoot and root. Also, malondialdehyde content and proline and chlorophyll in leaves were determined. The results showed that the activity of all antioxidant enzymes (except for SOD in roots) were increased significantly in plants under salt stress, and ascorbic acid application, as a antioxidant decreased their activity in leaves, but in roots it was not effective. Total protein content in leaves and roots decreased significantly under salinity condition. Ascorbic acid treatment increased total protein content under salt stress in both roots and shoots. Measurement of malondialdehyde content in leaves and roots showed that lipid peroxidation was increased by active oxygen species due to salt stress, and ascorbic acid reduced lipid peroxidation only in leaves. Chlorophyll content was also decreased by salinity compared to control. According to the result of present study, ascorbic acid application decreases deleterious effect of salinity.

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To study the effect of nitrogen, salinity and organic matter on growth and root morphology of pistachio (Cv. Badami), a greenhouse experiment was conducted. Treatments consisted of four nitrogen (N) levels (0, 60, 120 and 180 mg kg-1 soil as urea), four salinity levels (0, 800, 1600 and 2400 mg NaCl kg-1 soil) and three organic matter (OM) levels (0, 2 and 4% soil as farmyard manure). Treatments were arranged in a factorial manner in a completely randomized design with three replications. As the salinity levels increased, leaf, stem and root dry weights and root density, were significantly reduced. Addition of N up to 120 mg kg-1 soil, had no significant effect on growth, but the highest N level (180 mg kg-1), due to nutrient imbalance, reduced abovementioned plant parameters. In low salinity levels, N application improved the growth of pistachio seedlings, but at the highest salinity level, N addition didn’t suppress the adverse effects of soil salinity. Due to useful physical and nutritional properties of soil organic matter, addition of OM significantly increased leaf, stem and root dry weights, stem height, shoot/root ratio and root density.

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Chlorine and sulfate toxicity in water and soils are the main factors limiting growth and yield of most plants. Tolerance and sensitive indexes related to Cl and SO4 in irrigation water and effect of nitrogen fertilizer on barley were evaluated in a completely randomized design with three replications, under greenhouse conditions. Quantity salinity tolerance and susceptibility indices such as Mean Productivity (MP), Tolerance Index (TOL), Geometric Mean Productivity (GMP), Stress Susceptibility Index (SSI), Reduction Yield Ratio (Yr) and Stress Tolerance Index (STI) on the basis of plant yield with stress (Ys) and without stress (Yp) conditions were determined. Results showed that STI had a positive and highest significant correlation with grain and straw yields, compared to other indexes. Study of Standard Beta contents in grain and straw with STI index showed that the impact of Cl had a greater effect on reduction of salinity tolerance than SO4. Also Cl/SO4 ratios of 1:3 and 1:2 for grain and 1:2 and 1:1 for plant straw brought about highest tolerance to salinity, compared to non stress conditions. The scatter plot also confirmed such findings.

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Similar to plants, soil salinity may reduce microbial growth and activities in different ways. The aim of this study was to determine the effects of different levels of salinity on some microbial indices in the presence and absence of plant's living roots. In this study, five levels of salinity using NaCl, CaCl2, MgCl2 and KCl and three soil media (soil with no plant, soil cultivated with wheat and clover) replicated three times consisted our factorial experiment arranged in a completely randomized design. Results show that salinity caused a significant reduction in accumulated microbial respiration, microbial biomass carbon, substrate-induced respiration, and carbon availability index in uncultivated soil and in the soils planted with clover and wheat. Results also show that salinity caused a significant increase in metabolic quotient (qCO2) in uncultivated soil, and soils planted with clover and wheat. Microbial activity of cultivated soils at high salinity levels was almost similar to that of the uncultivated soils. We observed a small difference in soil microbial activity among the three media at high salinity levels, indicating the role of indirect effects of salinity might be less important with increasing salinity levels. We also found out that at low salinity levels, the available carbon was not a limiting factor for soil microflora, while at high salinity levels the activity of soil microbes might be carbon-limited. The lower values of qCO2 in cultivated soils compared with the uncultivated soil support the positive influence of root and its exudates on soil microbial activity in saline soils. The existence of plants in saline environments may help in alleviating the detrimental influence of low to medium salinity on most soil microbial activities, likely via the added root exudates and root turnover.