JWSS - Journal of Water and Soil Science

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In 1998 and 1999, adult population density of pistachio psylla and its sex ratio were determined weekly in two orchards with yellow sticky board traps and limb jarring. Also egg and nymphal population was determined on the leaves. By studying the seasonal population fluctuaions, the efficiencies of the two methods were compared. Meanwhile, using yellow sticky boards, diurnal activity of adults was investigated. Adult pistachio psylla became active during flower and leaf bud opening. Adult capture with both methods showed a high correlation with egg and unwinged nymphal density counts, with at least five peaks (maximum six). Two peaks occurred during spring and densities were much lower in comparison with those of later generations. First adult capture was observed on yellow traps. Yellow traps were found to be a better method than the limb jarring method for the study of seasonal population from adult captures. Sex ratio was equal through out the growing season and during the warmer or cooler months of the year. However, more males were attracted to traps and more females were captured with limb jarring early in the season. Peak activites during day occurred through 9-16 p. m.

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Color sticky board traps are used in pest management for capture of adult insect, to estimate and predict population densities in later stages. Yellow sticky board traps showed high attraction for pistachio psylla. It is important to study these relationships for the right placement of traps. For each of three repetition in one year, four pistachio trees were selected randomly in one orchard. Eight traps were located on two heights and four cardinal directions of each tree (32 traps in each repetition). After one week the adult psylla captured were counted. One and two weeks 1ater, eggs and nymph densities were counted on five leaves in one of the eight positions of each tree on four other randomly selected trees (total 160 leaves each time). Data were subjected to analysis of variance. Coefficient of variation was determined between adults captured in each position and eggs and nymph density counts. There were significant differences for adult capture and egg density among directions, and in most cases significant correlations were observed between adults captured on each position and total egg and nymph density count on trees. It was concluded that for egg and nymphal density estimation, we can place traps on either of two heights and four directions except on lower east part, but preferably on either height in south direction of the trees . Also suggestions for egg and nymph sampling are given in the text.

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Pistachio oyster shell scale is one of the important pests of pistachio in Iran whose females are reported to exist in two or three forms, each form developing on a specific part of the pistachio tree. In this study, abundance of the pest is investigated on leaves and branches for different ages, two heights and four cardinal directions of tree crown. Paralelly seasonal population fluctuations of three forms were studied on leaves, and one-and two-year-old branches separately. This insect overwintered as female mostly on one and two-year-old branches. It had two separable periods of activity, through the season. In the first period, eggs mostly hatched in April and crawlers mostly settled on leaves, fruits and newly grown twigs but rarely on older branches.These populations are male and female. In the second period, eggs mostly hatched in June, and crawlers settled mostly on leaves and one and two-year-old branches, but all individuals settled on leaves are males and those on branches are females. Population density is significantly higher on upper surface of leaves than on the lower surface. Analysis of variance showed that only significant differences existed in densities observed between trees in both generations, and also between two heights of tree crown for populations settled on wood. Two predatory species from Cybocephalidae and Coccinellidae, one ectoparasitoid from Aphelinidae and two endoparasitoids from Encyrtidae were collected as natural enemies of this pest in Isfahan.

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High attraction is reported for yellow sticky traps to capture pistachio psylla. In pest management, it is important to forecast from the adult population density the density of other developmental stages in order to gain an estimate of the density in damaging stages. In 1998 and 1999, yellow sticky boards with 10150.15 cm were installed in two pistachio orchards in Borkhar district of Isfahan and egg and nymphal densities were simultaneously counted on leaves. Collected data were used to determine regression relationships between two weeks’ average egg density, first and second nymphal instar densities and the sum of egg and first and second instar nymphal densities, one week after the average adult capture in two previous weeks. A low correlation was found between egg count and adult capture, but higher coefficients were obtained between other stages. It was possible to estimate first and second instar nymphal populations from the following equations:

 For high adult densities (X):

Y= 58.6+0.4762X-(7*10^-5)X²                                    R²=0.82                             commercial orchard

 Y= 27.68+0.5092X-(5*10^-5)X²                       R²=0.86                              abandoned orchard

and for low adult densities (X)

Y= 1.7162X-17.454                                     R²=0.97                              commercial orchard

 Y= 1.1117X-4.9841                                    R2=0.90                              abandoned orchard

The application of this method is recommended for the management of pistachio psylla.

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The coccinellid Cryptolaemus montrouzieri Muls. is an oligophagous predator that is used to control mealy bugs in the orchards and greenhouses. Citrus soft scale, Pulvinaria aurantii, as an active pest on citrus plants in North of Iran is also attacked by this predator. This research was conducted to study biological characteristics of C. montrouzieri on citrus soft scale and mealy bug, Planococcus citri. Initially, Cryptolaemus eggs were collected from colonies of two preys and reared in laboratory (14 L: 10 D, 25±1˚C, and RH 70 ±5%). The incubation period of eggs, 1^st, 2^nd, 3^rd and, 4^th larval instars, and total larval, prepupal, and pupal stages were studied on two preys. The developmental stages on two preys were not significantly different, except for the 4^th larval instar at 1% and total larval stages at 5%. The sex ratio of male to female on two preys were not significantly different at 1% and was 1:1. Except for pre-oviposition period, other parameters including female longevity, average number of eggs laid per day, average total number of eggs laid per female and percentage of hatching were significantly different at 1% on the two preys. The average feeding of total larval period on eggs of mealy bug and citrus soft scale were 5715 and 7694, respectively that, the difference was significant at 1%. The results showed that some of the biological characteristics of Cryptolaemus on two preys were significantly different. Since the predator feeding on the eggs of citrus soft scale was higher than the mealy bug, it seems that this beneficial insect could be used for biological control of the citrus soft scale.

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Elm leaf beetle, Xanthogaleruca luteola (Müller), life and fertility tables were investigated under laboratory conditions (25±2°C, 70±5%R.H. and 16L: 8D), on Ulmus carpinifolia, U.c.var.umbraculifera, U. glabra var. pendula and Celtis caucasica, in spring and summer. Since the experiments were conducted under controlled conditions, feeding on hosts with different nutritional qualities was considered to be the only cause of mortality. The aim of this study was to determine the susceptible hosts to the pest. The initial population for each life table was 100 first larval instar on 20 cm foliage which continued to the end of adult longevity. For larval and adult feeding, the foliage was replaced regularly. Larval and adult mortality and eggs number per female were recorded on a daily basis. Results showed that the net reproductive rate (R₀) and intrinsic rate of increase (r_m) were higher on U. carpinifolia than the other hosts in both seasons. The net reproductive rate was less than one on Celtis caucasica in spring and on U. c. var. umbraculifera in summer, which represented the negative population growth on these hosts. Therefore, U. carpinifolia was the most susceptible host to Elm leaf beetle, the other susceptible hosts being U. glabra var. pendula and U. c. var. umbraculifera, in a descending order and Celtis caucasica was the least susceptible one. .

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Populations of Arboridia kermanshah Dlabola is increasing in Isfahan vineyards. In this study, some morphological characteristics of the immature stages, over wintering sites, number of generations per year, and seasonal population fluctuations of this pest were studied in vineyards in Isfahan Province. Eggs showed apparently four distinct embryonic developmental stages. At 25.8 ±2˚C, development of eggs was completed in 10.9 ± 0.7 days. There were five nymphal developmental stages. Nymphal stages can be distinguished with wing pad development and width of head capsule and width of pronotum. The grape leafhopper overwintered as adults under leaves and crop residues. Rearing studies showed that the grape leafhopper had three generations per year, each lasting 33.9±0.6 (without overwintering period), 46.3±0.7 and 37.3±0.6 days, respectively. Seasonal monitoring of adults, nymphs and eggs showed three or four overlapping periods of activity in the field. Four periods of activities of adults were recorded through season from early May – mid June, mid June – mid July, mid July - late August and early September – mid October.

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Elm leaf beetle were reared under controlled conditions (25± 2 ^oC, 70± 5%R.H. and 16L: 8D) to determine relationship between biological traits and the number of eggs per female on different hosts and to evaluate correlation between traits. U. carpinifolia, U .c. var. umbraculifera, U. glabra var. pendula and Celtis caucasica were examined as hosts. The biological traits consisted of 1^st, 2^nd and 3^rd larval developmental times first, second, and third larval percent mortality rates prepupal and pupal developmental times male and female longevity and pre-ovipositional period recorded during experiments. The relationships between traits and the number of eggs per female were determined by multiple regression (Foreward selection and stepwise). The correlation between traits was evaluated, too. The results showed that the number of eggs per female were mostly affected by certain special traits such as 2^nd and 3^rd larval developmental time, pre-ovipositional period and male longevity which are distinct in different hosts and seasons.

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Some bioecological aspects of grape leafhopper, Arboridia kermanshah Dlabola, including: the egg distribution under the leaf surface, eggs and nymphs spatial distribution on the vine arms, the adult distribution on three strata of the vine canopy, the adult diel activities and the geographical distribution of this species in Isfahan province were studied. Samplings were done in the 1997. The egg distribution under the leaf surface was determined by repeated sampling during the season and the egg density was estimated on three regions such as the main and secondary vein, the leaf surface margins and others. Eggs and nymphs spatial distribution along the vine arms were studied weekly for six weeks by sampling an arm of five vines during the second generation, the leafhopper population was the highest. The adult spatial distribution on the vine canopy was determined by sampling three strata of the vine canopy using the D-vac apparatus. Diel activities were studied by sweeping nets fifty times per vineyard at the two hour intervals from 8 a.m. to 20 p.m. Temperature and the relative humidity were also measured in the shade once per hour. The presence and the abundance of this species in 87 vineyards in the different climatic conditions were studied by a sweeping net. The leaf margin and around of the secondary vein were preferred for the oviposition of the leafhopper. Adults, nymphs and eggs were more abundant in the middle strata of the vine canopy than others. The mean pattern of the daily activity of the adult indicated one peak at 8 a.m. and the other one at 20 p.m. Maximum temperature thereshold for the adult activities was 28º C. The study of the grape leafhopper distribution in isfahan province indicated that this species was distributed in the most vineyards, but was highly abundant in more humid conditions.

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In 1997, in two vineyards of Isfahan university of technology and Zobeahan in Isfahan, the density fluctuations of Arboridia kermanshah Dlabola and their injury symptoms were studied. In each vineyard three leaves of each of ten randomly selected vines were sampled weekly. Nymphs in different instars were counted directly and rate of injury symptom (leaf discoloration) was determined with planimeter. Special cages were used to determine rate of injury symptom for each nymphal instar in comparison to each other. Rate of injury symptoms and nymphal density indicated two peaks through growing season until harvest. Tolerance threshold for A. kermanshah nymphs was determined 80 first instar nymphal days or presence of 11-12 first instar nymphs and feeding for seven days. With increase of nymph densities, damage symptom increased rapidly.

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Fecundity and sex ratio are the important factors in the quality control of parasitoid, Trichogramma. In this research, the effect of the host egg deprivation on these factors in Trichogramma brassicae Bezdenko was evaluated. The experiment was conducted with four treatments, each in five replications in a completely randomized design. Twenty mated females (24 h old) were transferred into the glass tubes (1.5×10 cm) separately and were fed by honey 20% daily. In one treatment, parasitoids were provided with the 150 host eggs (Sitotroga cerealella) in first day, but in other treatments the 150 host eggs were offered after 2, 4 and 6 days. In order to obtain fecundity, black eggs were counted after 3 or 4 days. After emergence, adult parasitoids were recognized as male and female by their antennae and sex ratio was obtained. Results indicated that as parasitoids, Trichogramma sp., were deprived from the host eggs longer, more decrease was observed in fecundity and female ratio. Therefore, parasitoid quality was decreased by the host egg deprivation.

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The predatory beetle Cybocephalus fodori minor has recently been reported in Iran. Also few studies have been reported on life cycle of Cybocephalus beetles, especially on that of C. f. minor. Thus, the life cycle and feeding rate of this predatory beetle was studied on pistachio oyster shell scale, Lepidosaphes pistaciae under laboratory conditions (temperature: 25 ± 2 °c relative humidity: 65 ± 5% and photoperiod: 16:8 L: D/h). Life cycle of C. f. minor was completed at six stages including egg, three larval instars, pupa and adult. Duration of the development for one generation (not considering preoviposition period) was 43-46 days for males and 38- 42 days for females. The developmental time length for the egg, larval instars1-3, pupa, male and female adults longevity, preoviposition and postoviposition periods was 8.8 ± 0.1, 5.2 ± 0.1, 34 ± 0.1, 6.8 ± 0.1, 17.3 ± 0.3, 59.2 ± 2.2, 65.8 ± 3.1, 6.8 ± 1.6 and 3.5 ± 0.5, respectively. Average fecundidity (number of eggs laid by a female per her life time) was 64.7 ± 6.2. Sex ratio in the laboratory and in the field was 1:1. Daily feeding rate for the female and male adults, larval instars feeding on adult female scale was 7.1 ± 3.5, 6.9 ± 2.2, 1.8 ± 0.2, 3.7 ± 0.2 and 5.9 ± 0.3, respectively. These parameters were also determined when those stages were fed on the eggs and second nymphal stages of pistachio oyster shell scale. Adult beetles over 60 days exhibited the highest feeding on this scale.