JWSS - Journal of Water and Soil Science

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In order to produce Tristeza virus-free plants from Satsuma mandarin (Citrus unshiu) cultivars (Ishikawa, Sugiyama and Myogawa), unfertilized ovules of plants suspected of CTV (Citrus Tristeza virus), from Mazandaran Mahdasht orchard were cultured on 4 media.

 Embryogenesis of nucellus tissue of ovules from Ishikawa and Sugiyama cultivars on MS medium were 31% and 25% and on MT medium from Ishikawa was 40.5%. Unfertilized ovules of Myogawa did not germinate on all media.

Nucellar embryos of Ishikawa and Sugiyama developed on MT+GA3+ME were 83% and 95%, respectively. Embryo differentiation, leaf and plantlet formation were observed on MT+GA3+ADE+ME medium. In the stage of two leaves, the plantlets’ growth under 1500 lux illumination was twice its growth under 800 lux illumination. Budding of in vitro plantlets from both cultivars on susceptible to Tristeza and virus-free seedlings of Key Lime did not show any infection by Tristeza.

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The general characteristics of salt-affected soils and soil structure degradation process are partially known, but the effects of saline and sodic conditions on mechanical properties of soils are not well recognized. In this study, the effects of electrical conductivity (EC) and sodium adsorption ratio (SAR) on tensile strength of soils with different organic carbon contents were assessed under laboratory conditions. The soil samples were collected from Dasht-E-Naz, at Sari region in the North of Iran. The samples had the same clay mineral (Illitic) and the main difference between them was the organic carbon content, subjected to different cropping systems. The tensile strength was determined on soil samples which had been treated by solutions having defined EC (0.5 and 4 dS/m) and SAR (0, 5, 15). The tensile strength was positively related to organic carbon content, but negatively to SAR. With increasing SAR, tensile strength decreased, and at a given SAR, the treatments with higher EC showed higher tensile strength. The analysis of variance showed significant differences (at 0.01) between soil samples (four levels), soil sampling depth (two levels), EC (two levels), and SAR (three levels) for all variables under investigation. For soil factor, the order of averages were: Virgin soil > Permanent pasture (Festuca) > Intensive cropping > Permanent pasture (Agropyron).

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Whey powder, due to its lactose and protein contents, can be used as an important flour improver to decrease the rate of staling. In the present investigation, whey powder was added at 5 levels (zero to 5%, W/W) to the flour of Sangak bread. Bread was baked according to the formula and procedure used by traditional Sangak bakeries. Bread samples were kept at room temperature or under refrigeration for 36 hours. They were then evaluated for staling by a taste panel. They were also subjected to proximate analysis. The dough was examined for farinographic, amylographic, extensographic and fermentographic properties. The data of staling tests including color, texture, flavor and also proximate analysis data were analyzed by ANOVA. Finally, differences among treatments were examined by Ducan’s multiple range procedure. The results indicated that the best treatment was the use of 3% whey powder, as far as staling and texture of bread are concerned. However, the best color of bread was achieved with 5% whey powder so was true of the protein, ash and fat contents. Viscosity, as tested by amylography, and also parameters of farinography reached their highest levels in the dough prepared from flour having 5% whey powder in the formula. The best extensibility was observed with the flours containing 4% whey powder and the highest dough energy was associated with the dough prepared from flour having 5% whey powder. As for the loaf volume, best results were obtained with the dough prepared from flour having 3% whey powder. Overall, it is concluded that the use of 3% whey powder is the best treatment with regard to the delay in the rate of staling, increase in loaf volume, and color appeal of Sangak bread, and rheological properties of the dough.

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An experiment was carried out to estimate the potential activity of rumen anaerobic fungi in the degradation of dry matter and fiber of feeds. Samples of wheat bran, bagasse, cotton seed, alfalfa and corn silage were used as the substrates to culture rumen fungi which were isolated from a fistulated Shal sheep. Loss percentages of dry matter (DML), neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent (ADL), cellulose, and hemicellulose of samples were measured after 0, 3, 6 and 9 days of incubation. Dry matter and NDF loss of substrates varied from 10.6 % to 29.4% and 11.7% to 48.7% after 9 days of fungi growth. The highest and lowest DML and NDF were related to alfalfa and bagasses, respectively. The highest values for the ADF loss (39%), hemicellulose loss (65.6%) and cellulose loss (55.6%) were measured from alfalfa. The results indicated that rumen anaerobic fungi have the ability of degrading dry matter and fiber from different types of feed.

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In order to study the nectar quantity and quality of some onion cultivars and honeybees foraging activity on them, a complete randomized block design with three replications was conducted. Ten selected onion cultivars consisting of Ghom, Kashan, Azarshahr, Dorcheh, Tarom, Kazeroon 1, Kavar, Abarkooh, Hurand, and Yellow Sweet Spanish were used. Sixty mm mother bulbs of different onion cultivars were planted in 3×4 m plots on 6 lines. The distance between rows was 35 cm and plants were spaced 25 cm apart on each row. At flowering time, bees’ activity on the inflorescences was studied and flower nectar was collected in 10 micro-liter capillary tubes. Nectar volume was determined and analyzed. Mean numbers of honeybees visiting on inflorescence every 10 minutes and the mean residing time of each honeybee on a seed stalk (inflorescence) were significantly different in different cultivars. Hurand flowers had the least number of visitors and Dorche flowers had the longest visiting time. The amount of nectar varied in different cultivars, so did glucose (G), fructose (F), and sucrose (S) contents. Except for the Abarkuh cultivar that had more glucose than it had other sugars, all the cultivars had higher quantities of fructose than they had other sugars while sucrose had the least quantities among the sugars. Nectar sugar ratio in most of the cultivars was hexose dominant, which was identified by the low S/(F+G) ratio. Potassium, calcium, and sodium contents in nectar were different in different cultivars, too. Finally, the results showed that foraging activity of honeybees on onion inflorescences depends on the interactions of several factors such as nectar quantity and, more importantly, on sugar quantity and ratio as well as potassium content. .

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A 3×4 factorial experiment was conducted for 45 days to determine the effects of diets containing different levels of protein and fat on growth of fingerlings Beluga (Huso huso L.1758). Twelve different diets were formulated with 3 levels of protein (45,50 and 55) and 4 levels of oil including fish oil and sunflower oil (0.5 ,4 , 8 and 12). 1260 Beluga fingerlings with an initial body weight (IBW) of 2.2 gram, after adaptation to the experimental conditions, were selected and stocked randomly into 36 fiberglass tanks (100 water volume) at 35 fish per tank, and fed on experimental diets for 45 days. Two ways analysis of variance showed that the percentage of body weight increase (%BWI), specific growth rate (SGR),protein efficiency ratio (PER), food concumpsion ratio, and productive protein value (PPV) content were significantly (P<0.05) affected by different protein and fat levels. Fishes fed on diets No.3 (containing 45% protein and 14 % lipid) and No.8 (containing 50% protein and 17 % lipid) showed the highest increase in body weight percentage, the best specific growth rate, protein efficiency ratio, and lowest food concumption ratio. Besides, the highest productive protein value was observed in diet No.2 (45% protein and 10.5% lipid) and diet No.3 (45% protein and 14 % lipid).Comparison of chemical composition of examined fish corps indicated a significant difference in their protein and lipid contents (P<0.05). However, the composition of the diet, revealed no significant changes on the dry matter and ash content of the body. Based on the findings of the present study 45-50% dietary crude protein is recommended for the optimum growth of Beluga fingerling. Moreover, the rate of needed lipid for optimum growth of fingerling range is from 14 to17 percents.

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The fire blight with the bacterial causal agent, Erwinia amylovora (Burrill) winslow etal. is one of the most important diseases of the pome fruits that causes the economical losses to quince, pear and apple productions, respectively, in some parts of country. To determine the infection severity of the 43 raturally infected pear cultivars in collection of karaj horticulture research division and also for studing the reaction of these cultivars against disease, the evaluation was performed by the USDA standard system for these cultivars in collection. In this study, although the most of cultivars had been infected on the natural conditions, but disease severity was significantly different between them. By the USDA system, pear cultivars were divided in the 2 classes. Percentage of pear cultivars in very susceptible and moderately susceptible classes were 81.4 and 18.6 respectively. Also for dividing the pear cultivars, beside of USDA method, SPSS software and the cluster analysis were also by the UPGMA method and cultivars were divided into 3 clusters, but it seems that the classification of cluster analysis did not conform with USDA system. Correlation of I.V.S in the artificial tests and the disease severity by natural infection was very significant (r= -0.83).

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Increasing the water use efficiency through promotion of sprinkler irrigation system, in order to increase production, has been the aim of the Ministry of Agricultural-Jihad in the past decade. Considerable amount of investment and efforts have been devoted to this end. Therefore, investigation of these efforts and development of models to predict the adoption behavior of farmers are of considerable importance. A survey research was conducted in four provinces (Fars, Bushehr, Kohkiloye-va-Boyerahmad, and Chaharmahal-va-Bakhteyari). A stratified random sample of 422 farmers was interviewed including 124 and 298 adopters and non-adopters, respectively. The findings indicated that the discriminant model developed based on the multiplicity model is a better predictor of farmers’ adoption behavior than diffusion and farm structure model. Application of multiplicity model in extension of sprinkler irrigation can increase the adoption rate and as result the efficiency of extension efforts.

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This study was conducted in a sugar beet field at Collage of Agriculture Experimental Station, Ferdowsi University of Mashhad, Iran. In order to describe the pattern of spatial variations and density of Chenopodium album, Solanum nigrum, Amaranthus sp., Portulaca oleracea, Echinochla crus-galli, and Convulvulus arvense as the main prevalent annual and perennial weeds of sugar beet fields, geostatistic methods were used. Samples were taken by systematic method from the corners of (7m × 7m) grids, using (0.5m × 0.5m) quadrates in three stages (before application of herbicides, after herbicide treatment, and before harvesting sugar beets). The integrity of spatial variation of variables was determined by using variogram functions and distribution maps of species. The variograms indicated that variations of all variables did not happen by chance. The maximum and minimum ranges of variation were observed in Solanum nigrum (by 142.7m) and Portulaca oleracea (by 1.5m), respectively. Both maximum and minimum ranges of variations were related to pre herbicide application. The highest and the lowest spatial correlations were related to Amaranthus sp. (in the third sampling treatment) and Solanum nigrum (in the first stage of sampling), respectively. The spatial distribution maps confirmed the patchiness distribution of the weeds. The patch of weed was constructed from a dense point at the center, gradually tapering toward the edges. The patches were skewed across the rows and irrigation channels. The structure of patches altered during the growing season. Any information on the distribution of weeds in the fields can be useful to improve decision makings in relation to applying the herbicides, selecting the herbicide type or applying the amount of herbicide. Also it can be useful to better design of weed control programs.

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The present experiment was aimed to evaluate the freezing tolerance of two cold tolerant (MCC426 and MCC252) and a cold susceptible (MCC505) chickpea genotypes. The study was carried out in a split-plot factorial design with three replications. Factorial arrangement of genotype and acclimation (acclimation and non acclimation) were imposed as main plot and temperatures (0, -4, -8, -12, 16, -20ºC) as subplot. The effect of freezing temperature (FT) on plant survival was significantly different among genotypes (p<0.05). According to the average effects of acclimation and FT, the plant survival in MCC426 and MCC252 was 40% and 31% respectively more than in MCC505. Lethal temperature for 50% response (LT50) and temperature resulting in 50% lower dry matter (DMT50) in MCC426 were –10.8ºC and –8.4ºC, respectively and were lower than the other genotypes. Acclimation increased the freezing tolerance such that MCC426 tolerated up to –12ºC without any mortality, however, at this temperature, plant mortality rates in MCC252 and MCC505 were 25.7% and 67.7%, respectively. Plant regrowth was affected by the intensity of FT, such that plant dry weight (PDW) and stem height (SH) in –12ºC decreased about 63% and 50%, respectively, compared with non - frozen control plants. The most freezing damage was observed in MCC505, -12ºC treatment caused 90% decreases in PDW and SH, but at this temperature, PDW and SH in MCC425 decreased 55% and 49% and in MCC252, the reduction was about 60%and 54%, respectively. It seems that the use of controlled experiments would contribute to the evaluation of freezing tolerance and screening programs in chickpea germplasm for the estimation of LT50 and DMT50 .

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The effect of Rathayibacter tritici on the movement of Anguina tritici larva and nematode function as vector of ear rot bacterium was conducted in the laboratory (Agarose plates) and greenhouse conditions. The results showed that the contact of nematode larva with high concentration of bacterium or long duration of nematode-bacteria contact can decrease the movement and the efficiency of nematode function as the vector of the disease, and in some cases it resulted in the mortality of the nematode. No differences were detected in the mobility of larva in the concentrations less than 10² CFU and less than 0.5 hour of nematode-bacteria contact times and their controls (exposed to water alone). Movement of the nematodes appeared to be random under these conditions. It can be concluded that Rathayibacter tritici did not act as an attractant to Anguina tritici larva. These results suggest that the attachment of a large number of bacteria to nematode (as an essential vector of the bacterium) would induce nematode weakness and mortality. So it is possible that ear rot bacterium can parasite ear cockle nematode, or the nematode is a host for this bacterium .

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Genetic male sterility is controlled by one pair of ressesive allele (aa) in sugar beet. This trait is used in most breeding programes. The exsistance of the character in a line or population facilitates transfer of important trait to the breeding material (for example resistance to plant disease). Also, it is possible to increase genetic diversity of monogerm populations by using genetic male sterility. The time and cost of transferring of this gene will be decreased, if the character is tagged with a molecular marker. Bulked segregant analysis using 302 RAPD primers in two F2 populations (231 and 261 population) was performed for the the identification of RAPD markers linked to the genetic male sterility gene. DNA preparation from 8 male fertile and male sterile plants were separately mixed. At first, the primers were tested on bulks. The primers with polymorphic bands were tested on individual plants of the bulks. Only if the polymorphism of the primers was confirmed, they were tested on the other individual plants. Finally, 10 and 6 markers were identified in 231 and 261 populations, respectively, which their distances to male sterility gene were lower than 50 cM. AB-8-18-600r marker was the nearest marker to male sterility gene. This marker showed only 3 and 1 recombination in 231 and 261 populations, respectively. The distance of this marker and genetic male sterility locus was estimated as 5.3 cM in combined F₂ populations.

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A 4x4 factorial experiment with a completely randomized design with three replications was conducted separately on two major pistachio varieties, Ohady and Kaleghoochy, to investigate the effects of shaking amplitude and frequency on pistachio nut and cluster detachment. Four levels of shaking frequencies used for Ohady were 5, 7.5, 10 and 12.5 Hz, while those of Kaleghoochy were 7.5, 10, 12.5 and 15 Hz. Four levels of shaking amplitude for Ohady were 20, 40, 60 and 80 mm, while those of the Kaleghoochy were 40, 60, 80 and 100 mm. Analysis of variance and mean comparison showed that the effects of shaking amplitude and frequency on pistachio nut and cluster detachment were significant on both varieties. It was found that in Ohady, at frequency of 12.5 Hz and amplitude of 80 mm, the maximum effective nut detachment occurred. But considering the need to limit both yield loss and limb damage to 5% and minimizing the power requirement of the limb shaker, applying shaking amplitude of 60 mm and frequency of 10 Hz, which detaches 95% of the nuts, is considered as the most suitable combination of shaking amplitude and frequency. Also, at amplitude of 80 mm and frequency of 12.5 Hz, the highest percentage of cluster detachment occurred. Here again, by considering limb damage and power requirement, applying shaking frequency of 12.5 Hz at 60 mm amplitude with 69% cluster detachment was selected as the most suitable combination. In Kaleghoochi, at amplitude of 100 mm and frequency of 15 Hz, nut detachment was 100%, while the need to limit limb damage and power consumption, the combination of 60 mm amplitude and 15 Hz frequency with 97.2% nut detachment was selected as the most suitable one. For cluster harvesting of Kaleghoochy, shaking amplitudes of 60 and 40 mm and frequency of 12.5 Hz with cluster detachment of 75 and 69 percent, respectively were selected as the most suitable ones. The average ratio of tensile force required for removing pistachio nut to its weight (F/W), and the ratio of tensile force required for removing pistachio cluster to its weight (V/W), which could be considered as an index of ease of detachment, were measured 6.2 and 37 for Ohady and 9 and 37.8 for Kaleghoochy, respectively. Finally, we may conclude that by applying the suitable combination of shaking amplitude and frequency, shake harvesting of pistachio could be practiced successfully.

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In order to detect genetic variation of native fowls in Mazandaran native Fowls breeding station, blood samples were collected from 100 male and female of birds (1:11). The DNA of the blood samples was extracted according to an optimized salting out protocol. The extracted DNA was amplified through polymerase chain reaction (PCR). Of the twenty random primers (10 mer) were used in this study, fourteen yielded satisfactory PCR. The total 63 polymorphic and 77 monomorphic bands were detected for the 14 primers. The number of bands displayed for each primer ranged from 4 to 16 with 200-2100 base pairs. The highest and lowest percentages of polymorphism band were observed for primer 9 (72%) and primer 14 (16%) respectively. The band sharing frequency was calculated for each primer, which ranged from 79 to 96. The genetic similarity within population and genetic variation were estimated as 89 and 11 percentage respectively. In conclusion, the existence of high level of polymorphism after ten generation of selection may indicate the accuracy of genetic evaluation program, suitable selection strategies and also large enough effective population size in this breeding flock.

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  Citrus stubborn disease is caused by the helical mollicute Spiroplasma citri. The pathogen is naturally transmitted by sap feeding leafhopper vectors. In the present study attempts were made to detect S. citri in presumptive vectors. Leafhoppers were collected from various wild and cultivated plants in citrus growing areas in Jirfot. Fifteen leafhopper species were identified and 12 species were analyzed for the possibility of carrying S. citri with an antiserum prepared against a citrus strain of S. citri by indirect ELISA. S. citri was detected in leafhopper species by ELISA in Austroagallia sinuata, Psammotettix alienus, Circulifer haematoceps, Orosius albicinctus and Psamotettix striatus. However, S. citri was isolated only from the last three leafhopper species in LD10 medium. These leafhoppers were primarily captured from sesame fields. Therefore, these potential vectors and sesame farms may play a key role in the epidemiology of S. citri in the surveyed area.

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  Air temperature prediction models using satellite data are based on two variables of land surface temperature and vegetation cover index. These variables are obtained by atmospheric corrections in the values for the above data. Water vapor, ozone, and atmospheric aerosol optical depth are required for the atmospheric correction of visible bands. However, no measurements are available for these parameters in most locations of Iran. Using the common methods, land surface temperature can be measured accurately at 2 ° C. Given these limitations, efforts are made in this study to evaluate the accuracy of predicting maximum air temperature when uncorrected atmospheric data from the NOAA Satellite are used by a neural network. For this purpose, various neural network models were constructed from different combinations of data from 4 bands of NOAA satellite and 3 different geographical variables as inputs to the model in order to select the best model. The results showed that the best neural network was the one consisting of 6 neurons as the input layer (including 4 bands of NOAA satellite, day of the year, and altitude) and 19 neurons in the hidden layer. In this structure, about 91.4% of the results were found to be accurate at 3 ° C and the statistical criteria of R², RMSE, and MBE were found to be 0.62, 1.7 ° C, and -0.01 ° C, respectively.

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Six rice cultivars were crossed in half diallel design to evaluate their GCA and SCA in 2005. In the following year, parents and their progenies were grown in a randomized complete block design with three replications, and 10 traits were measured. The analysis of variance showed significant differences (p≤0.01) between cultivars. Furthermore, general combining ability (GCA) and specific combining ability (SCA) for parents and hybrids were significant. Therefore, additive and non-additive gene effects on controlling traits were demonstrated. According to the analysis based on the second and fourth Griffing methods, additive gene effects were more than non-additive gene effects on controlling growth period, plant height, panicle length, number of panicles/plant and brown rice length, while other studied traits were more controlled by non-additive gene effects. Comparison of the second and fourth Griffing methods showed that the proportions of additive and non-additive variances in two methods were different. Moreover, GCA and SCA resulting from the two methods for several traits such as vegetative and productive growth period, plant height, number of filled grains/panicle and grain yield were significant. Therefore, it could be concluded that using parental generations in the second Griffing method may cause biased estimate of the GCA and SCA variances. Thus, using the fourth Griffing method is more suitable than the other methods in providing time, cost and facilities, and is recommended as an applicable method.

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Root depth is one of the main parameters affecting soil moisture availability held within the plant root zone. This study was conducted to evaluate the trend of corn root development during the growing season in different irrigation methods with two water qualities, and distribution of roots in soil profile. The experiment was a split plot design with three main treatments (subsurface drip(SSD), surface drip(SD) and furrow irrigation(FI)) and two sub main treatments (municipal effluent and fresh water) in two consecutive years conducted in Korbal plain, Fars province. Root monitoring was carried out with the observation of soil profile root depth measurement. Samples were taken during the growing season 16 times, each with a 7 day interval. Measurement of root weight in different soil layers was carried out using monoliths of soil washed with water pressure using 50 mesh sieves. Although the longest roots were observed in plots irrigated with SSD and the shortest in FI, the final root length was not significantly affected by irrigation methods and water qualities. In this research, a model showing the trend of root length average growth was developed and tested in the experiment conditions. The average final main root depth for all treatments was 77 cm. Irrigation volume using root depth monitoring could be reduced compared to linear model, Borg & Grimes and CROPWAT model for root depth estimation. Irrigation water on the base of soil moisture deficit within the root zone in each irrigation event could be reduced when the real root depth is considered compared with the conventional general models giving a final root depth. Results showed the total dry root weight in SSD was more than that in SD and FI methods. However, water quality did not show a significant effect on dry root weight. The most root concentration in SSD was measured in depth of 20-40 cm, while in SD and FI the most concentration was in surface layer (0-20 cm). In the SSD, the roots were distributed more evenly compared with SD and FI methods. The concentration of roots in all treatments below 65 cm was not significant.

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The present study discusses a method used to produce updated information about vegetation cover in arid and semi-arid zones, using RS data and GIS technique. In this method, Landsat ETM⁺ data in 2002 was collected in an area of about 60000 ha in Nodoushan basin, Yazd, Iran. To collect the necessary ground data, 50 sites of different vegetation types were selected and the percentage of vegetation cover in each one was determined. Also, different vegetation and soil indices were derived and crossed with located sampling points using ILWIS software capabilities. To get the best fitted curve, the relationship between vegetation cover, as a dependent variable, and satellite data bands, vegetation indices and environmental factors, as independent variables were assessed. Therefore, a multiple linear regression model was established for the prediction of vegetation cover percentage in the studied area. Finally, a vegetation cover map with high a precision was produced. As a conclusion, it can be said that mapping of vegetation cover via remote sensing is possible even if its vegetation cover is sparse.

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In order to study agronomic and morphological traits in maize hybrids in Hammedan, two early (108 and 301), three medium (604, 647 and TWC647) and two late maturing (704 and 711) hybrids were evaluated in a randomized complete block design with three replications in Agricultural and Natural Resources Research Station in Hammedan in 2005. 33 morphological and phonological traits were recorded from 10 plants randomly selected from two central rows of each plot. The maximum and minimum grain yield was obtained from SC647 and SC301, respectively. The grain yield had the highest correlation with dehusked ear weight. Factor analysis of data after varimax rotation identified four factors that accounted for 98.03% of total variance. The scatter plot of hybrids based on the two first factors (the seed yield factor and phenological structure factor) showed that SC704 had the maximum forage yield and the best physiological characteristics and SC647 had the maximum grain yield, cob diameter and number of seed in row.