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Showing 14 results for Sepehr

M. Sepehri, N. Saleh Rastin, H. Asadi Rahmani, H. Alikhani,
Volume 10, Issue 1 (spring 2006)
Abstract

Heavy metals have deleterious the effects on nodulation and N2 fixation of Rhizobium- Legume symbiosis, due to their inhibitory effects on the growth and activity of both symbionts. This research has been undertaken to evaluate the effect of Cd tolerance of native rhizobial strains on diminution of the Cd detrimental effects on Sinorhizobium meliloti-alfalfa symbiosis. For this purpose, a greenhouse experiment was conducted based on Randomized Complete Block Design. The treatments in this experiment included: plants inoculation with 6 bacterial strains (sensitive, partially tolerant and tolerant to Cd), 5 levels of Cadmium (0, 2, 5, 10, 20 mg/kg soil) and non-inoculated control. In different levels of Cd, the effects of bacterial inoculation on root nodule number and total amount of nitrogen in plant shoot were compared. The results indicated that soil pollution by Cd even at 2 mg/kg had significant effect on symbiotic properties of rhizobial strains, and according to Cd tolerance of various strains, the mentioned effect was different. Decreasing effect of Cd concentration on root nodules and nitrogen concentration in plants that were inoculated with sensitive strains in comparison with plants inoculated with tolerant strains was 68.31% and 40.8%, respectively. In this research, R95m was introduced as the best strain because of its ability for nodulation and nitrogen fixation.
R. Sepehri, Gh. Tahmasebi, M. J. Jalali Zonoz,
Volume 11, Issue 41 (fall 2007)
Abstract

During the honeybee breeding project in central Iran, sex alleles homozygoty and sex alleles number in the third generation of 364 colonies were studied in 2003. Sex alleles homozygoty was measured based on Ruttner (1988) and Tarpy and Page (2002) methods. The total area of worker brood area, stored pollen, and drone brood area were measured on the combs. The whole extracted honey weight and remaining honey in the combs were evaluated as honey production of colonies. Adult population was evaluated based on the number of full combs of adult honeybees. The results showed that sex alleles, average homozygoty and number in the colonies were 18.83% and 5.32 respectively. The results showed no significant correlation between homozygoty of sex alleles or sex alleles number and stored pollen ( P > 0.05 ) , but significant negative correlation between sex alleles homozygoty and colony population, total brood area and honey yield ( P < 0.01 ) was observed. So introduction of new sire colonies in the mating Isolated area is essential to prevent sex alleles homozygoty and also performance of honeybee colonies from decreasing.
M. Karimi Kakhaki , A. Sepehri,
Volume 13, Issue 50 (winter 2010)
Abstract

In order to study the effect of deficit irrigation at reproductive growth stages on water use efficiency and drought tolerance of four sunflower cultivars, including Azargol, Allstar, Alison and Euroflor, an experiment was conducted during 2007 growing season at experimental field of Agricultural Faculty of Bu-Ali Sina University, Hamadan, Iran. The experiment was a split plot based on randomized complete block design with three replications. The irrigation levels included full irrigation, deficit irrigation at heading, deficit irrigation at flowering, deficit irrigation at seed filling, deficit irrigation at heading and seed filling and deficit irrigation at flowering and seed filling stages. The biological and economic yield (BY and EY), harvest index (HI), water used, water use efficiency (WUE) and water stress indexes were measured. The results indicated that the highest BY (11681.7 kg.ha-1), EY (4854.0 kg.ha-1) and HI (42%) were obtained from full irrigation treatment. The lowest negative effects in cultivars belonged to deficit irrigation at seed filling stage. Euroflor obtained the highest of these parameters with 10127.1 Kg.ha-1, 4081.5 Kg.ha-1 and 40% respectively. Highest WUE was related to twice cutoff of irrigation in flowering and seed formation stages and then without irrigation in seed formation stage by 1.09 and 0.96 Kg.m-3. Allstar and then Euroflor had highest WUE among sunflower cultivars by 1.01 and 0.94 Kg.m-3 respectively. Results also indicated that Euroflor was a tolerant cultivar and STI (Stress Tolerant Index) and GMP (Geometric Mean Productivity) were acceptable indexes for selection. Finally, deficit irrigation at seed formation stage had the lowest negative effect on yield and HI with suitable WUE. In addition, Euroflor showed the highest yield, drought tolerance and HI with suitable WUE.
N. Moradi, M. H. Rasouli-Sadaghiani, E. Sepehr, B. Abdolahi,
Volume 16, Issue 62 (Winte - 2013 2013)
Abstract

Organic acids can affect phosphorus (P) sorption. In order to evaluate the effect of citric acid and oxalic acid on phosphorus sorption characteristics in calcareous soils, five different soil samples (0-30 cm) were collected in West Azerbaijan province. Soil samples (2.5 g) were equilibrated with solutions of KH2PO4 containing 0 to 20 mg P l-1. Five mM organic acids were added to each tube and sorbed P was calculated. The sorption data were fitted to Langmuir, Freundlich and linear equations. The results showed phosphorus sorption data were well described by the Freundlich and linear equations. The average values of Kf (distribution coefficient), n and equilibrium buffering capacity (EBC) of linear equation in citric acid treatment were reduced to 29, 12 and 17 percent, respectively and for oxalic acid reduced to 43, 27 and 14 percent, respectively. Maximum sorption was reduced significantly by oxalic as compared to citric acid. Standard phosphorus requirement (SPR) was decreased by 57 % as a result of organic acids additions compared to control condition. It is concluded that organic acids additions to soils containing more calcium carbonate and high amount of clay can decrease P sorption.
A. Samadi, E. Sepehr,
Volume 17, Issue 65 (fall 2013)
Abstract

In order to determine optimum equilibrium solution phosphorus (P) concentration using P adsorption isotherm and obtain model(s) by integrating soil solution P concentration, physicochemical properties, and soil P test (available P) which predict standard P requirements to achieve maximum yield, laboratory and glasshouse experiments were conducted on 36 soil samples belonging to 15 soil series and 14 soil samples, respectively. Using wheat as a test crop, the glasshouse experiment was laid out with five P levels in a completely randomized design with three replications. Concentrations of P in solution established by adding P in the pots estimated from the sorption curve ranged from 0.2 to 1.2 mg P/L including check treatment (no P). The results showed that equilibrium solution P concentration (EPC) was almost low in comparison with the requirement for most crops (<0.2 mg/L). The amount of P adsorbed by the soils at 0.2 mg/L EPC ranged from 5 to 114 mg/kg soil. The phosphate adsorption was well described by Freundlich (R2 = 0.96) and Langmuir (R2 = 0.88) isotherms. Langmuir maximum adsorption (Xm) and Freundlich coefficient (aF) estimated from Langmuir and Freundlich equations ranged from 127 to 238 mg P /kg soil and from 43 to 211 mg P/kg, respectively. Yield of wheat in all soils approached maximum as adjusted P levels were increased to 0.4 mg P/L. The results showed that some soils studied were adequate in available P by the NaHCO3 test, but required an amount of P fertilizer by the isotherm P requirement test to obtain maximum biomass production. Soil clay content was significantly related to the soil P sorption indices, P0.4 (P sorbed at 0.4 mg P/L EPC) (R = 0.40, P<0.01), PBC (P buffering capacity) (R = 0.54, P<0.001), aF (R = 0.48, P<0.01), and Xm (R = 0.40, P<0.01). Total CaCO3 and Active CaCO3 were found to be less important factors affecting P adsorption. Using stepwise regression analysis resulted in a useful regression model including the combination of Olsen P and clay content for the prediction of standard P requirement (P0.4).
V. Jahandideh Mahjen Abadi, M. Sepehri, A.h. Khoshgoftarmanesh, H. R. Eshghizadeh, D. Rahmani Iranshahi,
Volume 19, Issue 71 (spring 2015)
Abstract

Zinc deficiency is the most widespread micronutrient disorder in the production of wheat (Triticum aestivum L.) and other cereal crops. An experiment was conducted in greenhouse, in 2013, using the sterile sand-perlite (2:1 v/v), to study the effects of two beneficial microorganisms on growth and nutritional status of wheat (Nicknejad cultivar). The study was arranged as factorial in a completely randomized design with three replications. The experimental factors consisted of Piriformospora indica (E0: Uninoculated E1: Inoculated), Pseudomonas putida (E0: Uninoculated E1: Inoculated) and Zinc (Zn0: 0 Zn1: 2µM ZnSO4 ). The results showed that inoculation by P. putida increased shoot dry weight at both levels of zinc, but this increase was observed for root dry weight only without zinc application. The iron concentration of shoot was decreased as a result of inoculation by P. putida at both levels of zinc. However, P. indica inoculation increased iron concentration in zinc application, but had no significant effect without zinc application. At both levels of zinc, the highest P, Zn, chlorophyll a and b concentrations were achieved by inoculation with P. indica. Inoculation by P. putida reduced P concentration at both levels of zinc but it reduced Zn, chlorophyll a and b concentrations only with zinc application. The results of this research showed that despite negative effect of P. putida on nutrient uptake, inoculation by P. putida and/or P. indica plays an important role in the promotion of wheat growth in zinc deficiency conditions.


F. Karimi, M. Sepehri, M. Afuni, M. A. Hajabbasi,
Volume 19, Issue 71 (spring 2015)
Abstract

By modifying plants at genetical, physiological and ecological levels, entophytic fungi as the most important soil microorganisms have a pronounced growth-promoting activity and also increase plant resistance to biotic and abiotic stresses. This research was undertaken to evaluate the potential of P. indica to increase barley (Hordeumvulgare L.) resistance to lead (pb). Therefore, a greenhouse experiment with two fungus treatments (non-inoculated and P. indica inoculated) and five levels of pb (0, 25, 50, 100 and 500 mg/kg) with three replications was conducted based on a factorial design. Measurement of shoot and root dry weight showed that the growth of P. indica-colonized plants at all levels of pb treatments was higher (P < 0.05) than that of the corresponding controls. Also, chlorophyll concentration of inoculated plants with P. indica was superior to non-inoculated plants. In addition, the results showed that in contrast to the plant shoot, lead concentration in the root of P. indica-colonized plants was higher than the non-inoculated controls.


M. Piri, E. Sepehr,
Volume 19, Issue 72 (summer 2015)
Abstract

Humic substances are the most important organic fractions in soils and have affinity towards trace metals. In order to evaluate the effect of humic acid on zinc (Zn) sorption and desorption by soil,‌ a batch experiment was conducted with two soil samples which were different in clay and calcium carbonate contents. Three levels of humic acid (0,‌ 200, 500 mg/L) and various Zn concentrations (0 to 450 mg/‌L) were applied at constant ionic strength (0.05 M NaCl). Adsorption data were fitted to Langmuir, Freundlich and Temkin equations. Freundlich equation fitted relatively better (R2= 0.86-0.98). Results showed that the application ofhumic acid increased both ‌sorption and desorption of Zn in the two sample soils. Increasing of humic acid concentration increased maximum adsorption of Langmuir (qmax) up to 8-21% and Freundlich sorption capacity (Kf) up to 73-95%.‌ All sorption parameters including‌ Langmuir binding energy (KL), Freundlich intensity factor (n) and coefficients of Temkin equation (A, KT) were increased by adding humic acid, so that the above mentioned sorption parameters of S1 (with low clay and calcium carbonate) were lower than those of S2 (with high clay and calcium carbonate). Application ‌of humic acid (500 mg/L) increased desorption of Zn (DTPA-Zn) ‌from 511 to 711 mg/kg for S1 and 499 to 609 for S2‌.
Mh. Rasouli Sadaghiani, S. Sadeghi, M. Barin, E. Sepehr, B. Dovlati,
Volume 20, Issue 78 (Winter 2017)
Abstract

Potassium is the most abundant nutrition element in the surface soil but most of the potassium is unavailable to the plants. The present study was conducted with the aim of isolation of potassium solubilizing bacteria from rhizosphere soil and evaluation of quantitative ability of released potassium from different sources of silicate by strains. For this propose, laboratory and greenhouse evaluations were carried out on corn (Zea mays L. Cv. single cross 640 (as a factorial in a completely randomized design with three replications. Laboratory factors were potassium sources (four levels), incubation time (seven levels) and microbial inoculation (six strains) and greenhouse factors were potassium sources (five levels) and microbial inoculation (four strains). The results showed that among the bacterial strains KSB13 had maximum dissolution diameter (25 mm) and solubilisation index (SI=3). The highest potassium content (3/32 µg/mL) was released from biotite by strains of KSB10 after ten days incubation. The microbial inoculation increased root dry weight and plant height for 30 and 25 percent, respectively, compared to control treatments. Also the mean shoot dry weight and K content in microbial treatments of silicate minerals were respectively increased 3/75 and 1/57 times higher than control treatment. It can be concluded that microbial inoculation causes potassium release from silicate minerals and improved plant growth.


Z. Sepehri, Z. Jafarian, A. Kavian, Gh. Heydari,
Volume 21, Issue 1 (Spring 2017)
Abstract

Ash and coal produced from fire influence the soil and few studies about these effects are available. For this purpose, this study was performed to investigate the effect of ash and coal on hydrological, physical and chemical properties of soil in Charat rangeland that has a history of fire. Systematic-random sampling was implemented in two plant types Astragalus gossypinus and Artemisia aucheri using 6 transects and 60 4m2 quadrates and plant and soil samples were obtained. Experimental treatments including control soil, composition soil and ash were prepared manually and also with artificial rain, composition soil and coal manually and also with artificial rain in the laboratory and characteristics of the texture, saturation moisture, pH, organic matter, field capacity, wilting point, available water and retention capacity were measured. ANOVA results for mean comparison soil, ash and coal properties showed that ash had more percent of silt and pH than soil and organic matter and lower bulk density than soil. In addition, multivariate analysis to show the effect of two types of treatments showed that manual composition of ash and coal with soil had increased field capacity and available water while composition of soil and coal with artificial rain had no significant effect on them. According to the results of this study, the presence of ash and coal resulted from burning vegetation caused great changes in soil properties, especially water retention.
 

F. Hosseini, M. R. Mosaddeghi, M. A. Hajabbasi, M. R. Sabzalian, M. Soleimani, M. Sepehri,
Volume 21, Issue 2 (Summer 2017)
Abstract

Soil water repellency can affect several soil properties such as aggregate stability. Soil texture and organic matter are two main internal factors responsible for the variability of soil water repellency. Major sources of organic matter in soil include plant residues, and exudates of plant roots and soil microorganisms. Tall fescue (Festuca arundinacea Schreb.) as an important cool-season perennial forage grass is usually infected by a fungal endophyte (Epichloë coenophiala) which often enhances resistance to biotic and abiotic stresses as well as altering the litter decomposition rate and soil properties. In this study, the effects of endophyte-infected (E+) and endophyte-free (E−) tall fescue residues (in three different levels of 0, 1 and 2%) on soil organic carbon, basal microbial respiration, water-dispersible clay and water repellency index (determined by intrinsic sorptivity method) were investigated in four texturally-different soils in the laboratory. E+ and E− tall fescue residues were completely mixed with moist soil samples and then were incubated at 25 °C. During two months of incubation period, the amended soil samples were subjected to 10 wetting and drying cycles and then, the above-mentioned soil properties were measured. The results indicated that soil organic carbon and water-dispersible clay were greater, while basal soil respiration and repellency index were lower in fine-textured soils. Water repellency index was increased by production of hydrophobic substances (for the rate of 1%) and was reduced by induced greater soil porosity (for the rate of 2%). Presence of endophyte in plant residues had no significant effect on water sorptivity, ethanol sorptivity and water repellency index; nevertheless, E+ residues increased soil organic carbon and decreased water-dispersible clay significantly. Overall, it is concluded that tall fescue residues, especially those with E+, can improve soil physical quality due to improving soil organic carbon storage and water repellency index and decreasing water-dispersible clay (as an index for aggregate instability). These E+ species and the residues have great potential to be used in sustainable soil conservational managements.
 


M. J. Asadollahzade, A. H. Khoshgoftarmanesh, M. Sepehri,
Volume 21, Issue 4 (Winter 2018)
Abstract

Iron (Fe) and zinc (Zn) deficiency is common in wheat growing areas of the world particularly in calcareous soils. Soil application of chemical fertilizers is considered as a cost-effective and easy approach to combat micronutrient deficiency. However, due to economic, environmental, and agronomic constrains, efficiency of soil fertilization is low in most calcareous soils. Therefore, finding proper and effective approaches to improve fertilizer use efficiency and/or soil availability of metal nutrients is of great importance. This research was performed to investigate the effect of endophyte fungus Piriformospora indica and Zn-sulfate application on root and shoot dry matter yield and uptake of Fe and Zn by wheat. The experiment was set up in a completely randomized factorial design; each treatment contained three replicates. Two wheat cultivars (Triticum aestivum L. cvs. Durum and Rushan) were exposed to two Zn fertilizer rates (0 and 15 mg/kg ZnSO4.7H2O) and were inoculated with and without P. indica. Results showed that inoculation with P. indica increased root and shoot uptake of Fe (25 and 27%, respectively) and Zn (46 and 26%, respectively). In general, inoculation of roots with P. indica resulted in significant increase of shoot and root dry matter yield and uptake of Fe and Zn by both studied wheat cultivars. Infection of wheat roots with P. indica seems to be an effective and environment-friendly approach to improve Fe and Zn uptake in calcareous soil; although further research is needed to clarify all aspects of this approach

J. Bayazzadeh, E. Sepehr, H. Momtaz,
Volume 23, Issue 2 (Summer 2019)
Abstract

To study the behaviour of phosphorus (P) sorption in the agricultural soils of Khoy region and the effect of long-term cultural management with the application of poultry manure on the P sorption parameters, bath experiments were carried out with 16 soil samples (8 cultivated and 8 virgin soils) and 9 initial P concentrations from 0 to 30 mg L-1 in 0.01M CaCl2 as a background solution. After equilibrium, the remaining amount of P in solution was measured and the experimental sorption data were fitted to the Langmuir (R2=0.93-0.99) and Freundlich (R2=0.87-0.99) models. The results showed that P sorption was increased with enhancing the initial P concentration, eventually reaching the steady-state plateau. Based on the coefficient of determination (R2) and the standard error of estimate (SE), both isotherms models, Langmuir and Freundlich, showed a relatively good fit to the experimental data. The maximum mono layer sorption of Langmuir (qmax) varied from 233 to 486 and from 340 to540 mg kg-1, and the energy parameter of Langmuir (KL) ranged from 0.12 to 0.50 and from 0.22 to 0.71 for the cultivated and virgin soils, respectively. Freundlich sorption capacity (KF) and intensity (n) parameters showed the same trends and KF varied from 36.4 to 123 and 59.3 to 145.2; also n varied from 1.18 to 1.50 and 1.47 to 1.71 in the cultivated and virgin soils, respectively. Consequently, all sorption parameters and the buffering indices showed a decreasing trend in the cultivated soils, as compared to the corresponding virgin soils and the cultural and fertilization management; especially, the application of the poultry manure in this region reduced phosphorus sorption by soil and then increased phosphorus availability to plants. Hence, less fertilizer would be needed to maintain a favourable P concentration in the soil solution for the optimum plant growth.

R. Mousavai, M. Rasouli Sadaghiani, E. Sepehr, M. Barin,
Volume 27, Issue 1 (Spring 2023)
Abstract

can provide useful information about P adsorption and the factors affecting it. A batch experiment was performed with phosphorus concentrations (0 to 35 mg/L) in two soils with different electrical conductivity (EC) (2 and 15 dSm-1) by a variety of biochar treatments including simple apple-grape biochar (BC), rock phosphate- biochar (BC-RP), enriched-biochar (BC-H3PO4-RP), enriched-biochar (BC-HCl-RP), triple superphosphate (TSP), and control (Cont). The results indicated that phosphorus sorption capacity varied between the soils. Biochar treatments were effective in reducing the phosphorus adsorption of both soils. Due to BC-H3PO4-RP and BC-HCl-RP treatments, the maximum phosphorus adsorption of soils decreased in S1 soil by 14 and 23 % and in S2 soil by 26 and 19%, respectively. Also, the use of these treatments decreased the parameters of Langmuir absorption intensity (KL) of S1 soil to 0.085 and 0.066, respectively and S2 soil to 0.11 and 0.15, L/mg respectively, and Freundlich absorption capacity (KF) of S1 soil decreased to 19.2 and 22.5 and S2 soil to 28.2 and 28.1 L/kg, respectively. Enriched biochars significantly reduced the buffering indices of both soils indicating phosphorus adsorption decreased and increased the availability of phosphorus for the plant. The standard phosphorus requirement of S2 soil was lower than S1 soil by both equations. Therefore, enriched biochar can be an effective strategy to increase phosphorus availability and reduce the use of chemical fertilizers in saline and non-saline conditions; however, more field studies are needed for a clear understanding of the potential of P-enriched biochar as a fertilizer alternative.


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