Showing 2 results for Chickpea.
H. Fallahi, M. Motallebi, M.r. Zamani,
Volume 10, Issue 4 (1-2007)
Abstract
Ascochyta blight caused by Ascochyta rabiei is one of the major diseases of chickpea (Cicer arientinum) in Iran. Many phytopathogenic microorganisms, incuding A .rabiei, attack their host plant by secreting pectic enzymes including polygalacturonase (PG) which causes modification of cell-wall structure, increasing accessibility of cell-wall components for degradation by other enzymes. Polygalacturonase is the major factor in the initiation of Ascochyta blight disease, therefore in this study, the enzyme was purified from a virulent isolate of A .rabiei (IK06). Fungi were cultured in PZ medium culture media were harvested and after dialysis used for purification. Purification was achieved by Carboxy Methyl Sepharose Fast Flow ion exchange column equilibrated to pH= 5.5. Zero to one molar NaCl gradient was used for elution of the proteins from the column. Determination of protein content and enzyme activity of each fraction showed that PG was eluted from the column in 0.3 to 0.4 M salt. The purity of the protein and the MW of the enzyme were determined using SDS-PAGE technique. The MW was found to be around 27 KDa. The activity of the purified protein was also evaluated using polyacrylamide gel containing pectin as substrate (zymogram gel). Optimum pH for the purified enzyme was 7.5.
S. Fallah,
Volume 12, Issue 45 (10-2008)
Abstract
To study the effects of planting date and density on yield and its components in chickpea (Cicer arietinum L.) genotypes under dryland conditions of Khorram-Abad, an experiment was conducted during 2005-2006 growing season at the Agricultural Research Station of Lorestan Weather Department. Three sowing dates (March 6, March 21, and April 5) and two chickpea genotypes (Greet and Flip 93-93) with four plant densities (18, 24, 30 and 36 plants m-2 ) were evaluated using a randomized complete block design with split-factorial design in three replications. Dates of planting were considered as the main plots, genotypes and plant densities were randomly distributed in sub-plots with factorial arrangement. The result showed that delay in planting from March 6 to April 5 significantly reduced number of pods per plant, number of grains per pod, 100-grain weight, grain yield and dry matter. Increasing plant density led to a significant decrease in number of pods per plant, number of grains per pod and 100-grain weight. However, grain yield and dry matter increased firstly with increasing plant density and then decreased. Planting date and density had no significant effect on harvest index, while genotype of Greet produced a larger number of pods per plant, grain yield as well as final dry matter. Also Flip93-93 produced a bigger number of grains per pod, greater grain weight and harvest index. The maximum grain yield was obtained with genotype of Greet at the first planting date in 30 plants m-2 density. It could be concluded that due to thermal and drought stress occurring in late spring,, early planting of Greet genotype at 24-30 plants/m2 may lead to a suitable increase in grain yield.
