JWSS - Journal of Water and Soil Science

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This study was conducted at the Research Farm of Isfahan University of Technology in 2003 to evaluate drought tolerance potential of 20 oat (Avena sativa L.) genotypes including 14 cultivars released in Canada, two cultivars released in the United States of America and Switzerland and four Turkish breeding lines. A randomized complete block design with three replications was used in each irrigation treatment (i.e., irrigation after 70±3 and 130±3 mm evaporation from class A pan). Results of analysis of variance revealed significant differences among cultivars for all studied traits in both irrigation treatments, with the exception of plant height at heading stage and biological yield in stress condition. Stress condition significantly reduced all the traits. Also, the interaction of genotypes by irrigation treatments was significant for days to heading, grain and biological yields and 1000-grain weight. To evaluate drought tolerance of the studied cultivars, Rosielle and Hamblin tolerance index, mean productivity (MP), Fischer and Maurer stress susceptibility index and Fernandez geometric mean productivity and stress tolerance index were calculated based on yield data in stress and non- stress conditions. According to the obtained results, stress tolerance index seems to be the most effective index to identify tolerant genotypes. Rank correlations of stress tolerance index and grain yields in stress and non-stress conditions were 0.93 and 0.78, respectively. Based on this index, Boyer and Paisley were found to be the most tolerant cultivars.

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Many experiments have been carried out to alleviate the negative effect of drought stress and to obtain suitable growth under water deficient condition. Application of plant growth regulators (especially growth retardants) is one of the proposed methods. In a greenhouse experiment using a completely randomized block design with 4 replications at Kerman University Agricultural Department, the effect of 4 drought levels 25, 45, 65, 85 percent of field capacity and 3 different concentrations of chlormequat chloride 0, 1500, and 3000 mg/liter on some factors of winter barley cultivar Valfajr was investigated. Results indicated that increasing the cycocel concentration from 0 to 3000 milligram per liter leads to significant decrease in plant height. Relative water content affected by growth retardants was increased. Numbers of tillers and root and shoot dry weight, were also decreased because of applying cycocel in dry condition. The result also indicated that the root/shoot dry weight ratio, grain number per ear and ear number in dry condition without stress would increase using cycocel treatment.

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Generation mean analysis (GMA) was used to study the type of gene action and inheritance of grain yield and its components. Generation mean analysis with joint scaling test was performed. This research was conducted at research farm of college of Agriculture at the University of Tehran. The parents and their progenies ( F1, F2, BC1 & BC2) in three mating groups ( Sardari × 7007, Sardari × 7107& 7107 × 5593) were produced and planted using a randomized complete block design with four replications for each mating group. Of four replications in each experiment, two replications for drought and two replications for non-drought condition were used. The eight traits which were evaluated included grain yield, plant height, plant weight, tiller number, spike length, grains per spikes and 100 grain weight. Most of the genetic parameters including mean (m), additive (d), dominant (h), additive × additive [i], additive × dominant [j], and dominant × dominant[l] effects were significant. However, all gene effects were not significant in all traits.The dominant gene effect was the most contributor factor to inheritance of the majority of traits. For the majority of the traits, additive gene effect was significant, but its magnitude was less than dominant gene effect. Also the dominant × dominant[l] epistasis was more important than additive× additive [i] epistasis. The degree of dominance in most of traits indicated the predominance of dominant gene effects.

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In order to examine the responses of proso millet (Panicum miliaceum) to drought stress at different growth stages, four breeded genotypes and a local one of proso millet were selected and planted in a split-plot design with five irrigation treatments and three replications.This experiment was conducted in two locations, Birjand and Sarbisheh. Irrigation treatments included well - watered, drought stress at vegetative stage, ear emergence stage, seed filling stage and vegetative and seed filling stages which were considered as main- plots. The first five mentioned genotypes were considered as sub-plots. Drought stress. caused a great reduction in grain yield and WUE at ear emergence stage. This reduction represented itself in the number of seed per ear and the weight of seeds, but it didn’t have any effects on the number of ear. At ear emergence stage, the drought stress increased the floret death and loss of weight of seeds which resulted in the reduction in the harvest index of both ear per plant and seed per plant. Comparison of genotypes indicated that since K-C-M.4 had a greater number of ears and K-C-M.9 had heavier seeds that had higher grain yield. These two genotypes had the highest WUE and their harvest indices were relatively higher. Due to the salinity of water and infertility of soil in Birjand, the grain yield was lower compared with Sarbisheh. In summary, it can be said that genotype K-C-M.4 proved to be more suitable for both areas.

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Drought stress is one of the major limiting factors of crop yield. A field experiment was conducted to study the effects of drought stress on yield and yield components of nine bread wheat and one durum wheat genotypes during 2003-2004 growing season. The experiment was designed as two separate randomized complete blocks design with three replicates under well-watered and unwatered conditions during the post-anthesis period. Post-anthesis drought stress reduced the grain yield and yield components in all genotypes. Mean of each trait significantly(p≤%5) decreased under drought stress conditions, except for spikeletes number per spike and ear number per square meter. The highest yield loss was caused by the grain number per ear and 1000- grain weight reduction under drought stress conditions.“Gahar” genotype had the highest grain yield (4149 kg/ha) under drought stress conditions, whereas the highest grain yield (6674 kg/ha) was obtained for “Nikenejad” genotype under optimum conditions. Based on stress susceptibility index, “Koohdasht” and “Boholh-15” genotypes showed the lowest and the highest yield loss in response to drought stress, respectively. Correlation analysis of yield and yield components indicated that, under drought stress conditions, ear number per square meter (r=0.751) and biological yield (r=0.707) had the highest correlation with the grain yield(p≤%5), whereas these traits were grains number per ear(r=0.864) and biological yield (r=0.848) for the well-watered conditions. Therefore, it might be possible to select genotypes for greater grain yield with the means of the traits which have the highest correlation coeficient with the grain yield under each moisture regime. Generally, it appeared that “Gahar”and “Nikenejad” genotypes with greater yield potential might be recommended for agronomic conditions similar to the present investigation.

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To improve a complex character such as yield with low heritability, the use of indirect selection through other characters and a selection index based on different effective traits is recommended. This study was conducted to evaluate different selection methods by using 23 F2:4 wheat lines derived from the cross of Virmarin (susceptible cultivar) and Sardari (tolerant cultivar) at Research Farm of Isfahan University of Technology. A randomized complete block design with three replications was used in two irrigation treatments (i.e. irrigation after 70±3 and 120±3 mm evaporation from class A pan). For each irrigation treatment, different selection indices were constructed based on the number of spike per m², days to heading, number of grain per spike and 1000 – kernel weight. Also direct and correlated responses were calculated for these traits. The results of the response to selection and correlated response indicated that in the same conditions of this study, early heading cultivars with high number of spikes and low number of grain per spike with high 1000-kernel weight will have more yield potential. Number of spike per m² and 1000-kernel weight exhibited positive response in most of the indices and both irrigation treatments, while days to heading and number of grain per spike revealed negative responses. Thus selection based on these indices would result in early heading cultivars with high spike per m2, low number of grain per spike and high 1000–kernel weight. It was also found that Smith-Hazel and Brim-Williams indices had the highest efficiencies.

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Drought is a major factor limiting growth and development of crops such as mung bean (Vigna radiate (L.) wilczek) in arid and semi-arid regions of the world. This study was conducted to investigate the effects of different timing and severity of drought stress on physiological traits of mung bean and its relation to grain yield. A field experiment was carried out during 2004 growing season at Experimental Farm of Agriculture College, University of Tehran, Karaj, Iran. The treatments were laid out in a Randomized Complete Block Design (RCBD) with three replications. Plants were exposed to moderate and severe water stresses at either vegetative (VS) or reproductive stages (RS). Physiological traits were measured at the end of vegetative and the middle of pod formation. Generally water stress reduced leaf net photosynthesis rate, stomatal conductance and leaf relative water content at different growth stages. The effects of RS treatments were more severe than that of VS one. Severe VS treatment increased photosynthetic water use efficiency, whereas RS treatments decreased it significantly. However, leaf area index and total dry matter were more responsive in VS compared to RS treatments. VS treatments did not affect harvest index, while RS treatments reduced it significantly. Drought stress also reduced grain yield by 9 and 49 % (relative to control plants) in severe VS and severe RS treatments, respectively. Therefore irrigation is critical during reproductive stage mainly because of the higher demand for photoassimilate. It is concluded that to maximize mung bean grain yield in arid and semi-arid areas, appropriate watering should be practiced across all phenological stages in general, and during reproductive stage in particular.

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The increasing cultivation of corn in Ahwaz and the direct relation between the increase of the summer corn yield and its perfect irrigation in sowing period all together have made the research regarding this crop necessary. Deciding over the suitable irrigation scheduling of corn (hybrid SC.704) in summer by utilization of class A evaporation pan is the focal point of this research. Accordingly, a project was conducted in the form of stadistical perfect accidendal block in four repetitions and four treatments in 1383 on Ahwaz Azad university research land situated in Choneibeh. The irrigation treatment had four levels of T1,T2,T3 and T4 in class A evaporation pan containing of four levels of 50,75,100 and 150 mm, carried out in split plot. The suitable irrigation period was chosen according to the best treatment performance and its components, and the total amount of water in the taking period defines the depth of irrigation. The soil texture is clay lomy silty and the internal soil is silty clay. According to the amount of the accumulated evaporation from class A evaporation pan and taking the plant coefficient (Kc) into consideration, the amount of needed evapotrans piration was calculated and the amount of needed water for plot was measured by water counter. Then a comparison between the means by the use of Donken multi domains test was made and in this way the superior treatment was selected. Accordingly, the most suitable time for irrigation of summer corn in Ahwaz was after 50 mm of accumulated evaporation from class A evaporation pan which equals ten irrigation in the growing period. So in order to acquire 12 tons of seed yield in each hectare, at least 9600 m^3 of water for every hectar is needed. However, given the equal amount of water, with 3 times decrease in irrigation in treatment T2 in comparison with T1, a performance near 11 tons and with five times decrease in irrigation and ten tons in treatment T3 a performance near 10 tons can be attained.

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The effects of irrigation, dry farming and drought treatments on the activities of antioxidant enzymes including superoxide dismutase, ascorbate peroxidase, catalase and peroxidase in barley leaves at different generative growth stages under field conditions were investigated. Three senescence parameters including chlorophyll, total soluble protein and rubisco large subunit protein loss, were also studied in order to compare our results to those reported by other researchers. The results showed that leaf relative water content (RWC), chlorophyll and total soluble protein and rubisco large subunit protein content declined with leaf age and the effect of water deficit. The activity of superoxide dismutase declined with the progress of the leaf age on all treatments but ascorbate peroxidase activity declined with leaf age only in irrigated (control) plants. There were no significant differences among developmental stages in catalase activity in control plants, while catalase activity declined in the water dry farming and drought stress conditions. Peroxidase activity increased with the progress of senescence for all of treatments in such conditions. Water deficit stress triggered increases in antioxidant enzymes activities. Results showed that among all studied enzymes, peroxidase has a key role in increasing resistance to oxidative stress on both the senescence stages and drought stress condition in Hordeum vulgare.

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The objective of this study was to evaluate some morphological characteristics associated with grain yield potential on 20 bread wheat cultivars under two irrigation and drought stress conditions. The experiments were conducted at Research Farm of Agriculture College, University of Tehran, Karaj during 2001-2003. Twenty bread wheat cultivars were planted as subplots within each irrigation main plots in randomized complete block design with 3 replications. Drought stress was imposed at the end of flowering, and plants were re-watered when they showed signs of wilting, particularly during the morning. The results showed that the interactions between cultivars × year and cultivars × irrigation were significant for the grain yield. The cultivars showed significant differences in grain yield under drought stress condition in 2001-2003. Roshan, M-79-4 and Alvand in 2001-2002 and Azadi, Marvdasht and M-79-17 in 2002-2003 were most productive under drought stress condition, whereas C-79-12, MV-17 and Shahpasand in 2001-2002 and M-79-4, Roshan and Nicknajad in 2002-2003 performed visa versa. Correlation between grain yield and measured traits under irrigation condition was different from drought stress condition, suggesting that grain yield controlling mechanisms operate differently under these conditions. Drought stress caused a reduction in 1000 seed weight of cultivars. High 1000 seed weight and high grain number per ear were accompanied by high grain yield in drought stress and irrigation conditions, respectively. In the present study, clear relationship between grain yield and ear length was not observed.

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Canola (Brassica napus L.) as one of the oilseed crops has recently received lots of attention due to its suitable oil quality and high oil percentage. To evaluate the effect of water deficit stress on canola genotypes with regard to yield, drought tolerance quantitative indices and identify drought tolerant genotypes, an experiment was conducted in research farm of seed and plant improvement Institute (Karaj) using completely randomized block design with four replications, and 25 genotypes under two stress and non stress conditions. Genotypes were evaluated regarding drought tolerance. Drought tolerance quantitative indices include MP, GMP, STI, SSI and TOL. Result of variance analysis showed significant differences among genotypes as regards all indices and seed yield of genotypes in both conditions at 1% probability level. MP, GMP and STI indices were selected as the best indices for selection of tolerant genotypes in regard to analysis of correlation between seed yield in two stress and non stress conditions and drought tolerance indices. Results indicated that Genotype Vectra was the best genotype, because of its proper mean yield in both stress and non stress conditions. Multivariate biplot figure display showed that MP, GMP and STI indices had high correlation coefficient with each other, and tolerant genotypes were located near tolerance indices. Also results of three dimensional scatter plot and cluster analysis indicated that genotypes Modena, Jura, Eshydromel, Vectra, Dante, Zarfam, Esbetty and Olano were tolerant to drought stress, and genotypes Olpop, GKHelena, RG4504 and Olphi were sensitive genotypes.

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Osmotic adjustment is generally regarded as an important adaptation of wheat to drought. Because it helps to maintain turgor and cell volume, osmotic adjustment is often thought to promote growth, yield, or survival, of plants in dry soils. However, a physiological rationale for such views is lacking. This study was conducted to determine the effect of osmotic adjustment and some of its components on the wheat yield. Ten bread wheat cultivars were grown in pots under different watering levels using a split plot design. During grain filling stage, RWC, leaf sugar content, proline content and osmotic regulation were estimated by sampling flag leaf under normal and stress conditions. Main stem was used for measuring sugar content of different parts of stem. Although there were some differences in osmotic regulation in wheat lines, there were no relationships between yield and osmotic regulation. It was shown that these components probably have a little share in wheat osmotic regulation.

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Effect of drought stress and mycorrhizal symbiosis on growth and phosphorus (P) uptake by two sorghum cultivars different in root morphology was studied in a pot experiment. A factorial experiment with a randomized complete block design was performed. In this study, sorghum speed feed cultivar with high root proliferation and branching and sorghum KFS2 cultivar with low root proliferation and branching were colonized by Glomus intraradices. All plants were watered equally in the first three weeks to establish them. Drought stress was applied from the week four and the plants were watered when 40% (T1), 60% (T2) and 80% (T3) of the available water was consumed. Results showed that in both sorghum cultivars, plant dry weight decreased as drought stress was increased. However, dry weight of mycorrhizal plants was higher than that of non-mycorrhizal plants at all levels of drought stress. This increase for speed feed cultivar was greater than for KFS2 cultivar. Phosphorus concentration and total P uptake in shoot of both mycorrhizal plants were greater than those of non-mycorrhizal plants. P accumulation in both cultivars decreased by increasing drought stress due to the decline in plant biomass. However, P accumulation per unit length of colonized root increased by increasing drought stress. The greater percentage of root colonization and the increased total length of external hyphae per unit length of colonized root of KFS2 cultivar led to an increase in mycorrhizal growth response and improved P nutrition of KFS2 cultivar compared to those of speed feed cultivar.

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